Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,022,508	A→G	V459A (GTG→GCG)	yccS ←	putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,022,508	0	A	G	100.0%	217.4 / NA	58	V459A (GTG→GCG)	yccS	putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/31);  total (27/31)

TCACCCAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGA  >  NC_000913/1022373‑1022642                                                                                                                                         |                                                                                                                                       tCACCCAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGcgca                                                                                                                                      >  2:634448/1‑139 (MQ=255)     ccAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca                                                                                                                                 <  2:689820/139‑1 (MQ=255)       aGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACcaca                                                                                                                               >  1:696166/1‑139 (MQ=255)         tAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACcacagc                                                                                                                             >  1:252148/1‑139 (MQ=255)          aaGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACcacagca                                                                                                                            <  2:696166/139‑1 (MQ=255)              tAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATGGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAgg                                                                                                                        <  2:703848/139‑1 (MQ=255)               aaaaCACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGa                                                                                                                       >  2:969880/1‑139 (MQ=255)                       gTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc                                                                                                                  <  1:142133/136‑1 (MQ=255)                       gTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc                                                                                                                  >  2:142133/1‑136 (MQ=255)                       gTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAAt                                                                                                               >  2:1088772/1‑139 (MQ=255)                           cACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg                                                                                                           <  2:93177/139‑1 (MQ=255)                           cACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg                                                                                                           <  2:252148/139‑1 (MQ=255)                               aaaaGTGTGATGAACATCGTTGCATGAGTGTATTGCACGTTACGGAAGTCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTGCCAGTGATGGCGCAAATCACAGCACAGGGATGCCAAAGGCGAtac                                                                                                       <  1:254855/139‑1 (MQ=255)                                      tgatgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg                                                                                                           <  1:843018/128‑1 (MQ=255)                                      tgatgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg                                                                                                           >  2:843018/1‑128 (MQ=255)                                       gatgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAg                                                                                                <  1:634448/139‑1 (MQ=255)                                        atgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCTAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg                                                                                              <  2:676970/139‑1 (MQ=255)                                        atgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg                                                                                              >  1:1008418/1‑139 (MQ=255)                                        atgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg                                                                                              >  2:625570/1‑139 (MQ=255)                                         tgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGt                                                                                             <  1:17195/139‑1 (MQ=255)                                           aaCATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTAc                                                                                           >  1:591138/1‑139 (MQ=255)                                                cGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCaata                                                                                      <  1:664265/139‑1 (MQ=255)                                                     cATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAA‑CAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca                                                                                                                                 >  1:760109/1‑90 (MQ=255)                                                     cATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAA‑CAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca                                                                                                                                 <  2:760109/90‑1 (MQ=255)                                                       tGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGc                                                                                                            <  1:97159/110‑1 (MQ=255)                                                       tGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGc                                                                                                            >  2:97159/1‑110 (MQ=255)                                                          gCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCg                                                                                              >  1:762768/1‑121 (MQ=255)                                                          gCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg                                                                                              <  2:762768/121‑1 (MQ=255)                                                           cGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATgg                                                                                                             >  1:433258/1‑105 (MQ=255)                                                           cGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATgg                                                                                                             <  2:433258/105‑1 (MQ=255)                                                                 gCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCg                                                                     <  2:284591/139‑1 (MQ=255)                                                                  cACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCaat                                                                                       >  1:987732/1‑120 (MQ=255)                                                                  cACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCaat                                                                                       <  2:987732/120‑1 (MQ=255)                                                                    cGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTg                                                                  >  2:722112/1‑139 (MQ=255)                                                                       tACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGgcg                                                               >  1:994035/1‑139 (MQ=255)                                                                         cGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGcgcg                                                             <  2:335616/139‑1 (MQ=255)                                                                          ggAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATgg                                                                                                             <  1:299583/90‑1 (MQ=255)                                                                          ggAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATgg                                                                                                             >  2:299583/1‑90 (MQ=255)                                                                              ggCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca                                                                                                                                 <  1:984125/66‑1 (MQ=255)                                                                              ggCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca                                                                                                                                 >  2:984125/1‑66 (MQ=255)                                                                                 aaaaaaGAGGACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGACGGTGGCGCGTGGCGTta                                                     >  2:745712/1‑139 (MQ=255)                                                                                               c‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCa                                       <  2:1080612/139‑1 (MQ=255)                                                                                                        aCCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGacaaaca                               >  2:1057319/1‑139 (MQ=255)                                                                                                        aCCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGacaaaca                               <  1:1057319/139‑1 (MQ=255)                                                                                                            gcagcaCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACt                           >  2:294486/1‑139 (MQ=255)                                                                                                             cagcaCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATCGTTTGGCTGGCAGACAAACAAACtt                          >  1:1107437/1‑139 (MQ=255)                                                                                                             cagcaCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACtt                          >  1:101628/1‑139 (MQ=255)                                                                                                                 aCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCa                      <  2:870736/139‑1 (MQ=255)                                                                                                                   cAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGc                    <  2:591138/139‑1 (MQ=255)                                                                                                                    aGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCTAATGGCGATACCTACCAGCGTACCAATAATCGTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCa                   <  1:722112/139‑1 (MQ=255)                                                                                                                    aGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCa                   <  1:294486/139‑1 (MQ=255)                                                                                                                      cTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAg                 <  2:303140/139‑1 (MQ=255)                                                                                                                       tGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGa                >  2:347093/1‑139 (MQ=255)                                                                                                                       tGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGa                >  1:537553/1‑139 (MQ=255)                                                                                                                                   gATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGt    >  1:745265/1‑139 (MQ=255)                                                                                                                                    aTGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCtt                                                                                >  1:148429/1‑62 (MQ=255)                                                                                                                                    aTGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCtt                                                                                <  2:148429/62‑1 (MQ=255)                                                                                                                                    aTGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTg   <  1:577943/139‑1 (MQ=255)                                                                                                                                     tGGCGCAAACCTCAGCACAGGCATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCATACAAACAAACTTGTCATCAAGATCCAATACCCGTGa  <  1:1055884/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TCACCCAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCC‑GGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGA  >  NC_000913/1022373‑1022642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑