| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,532,523 | (A)7→8 | intergenic (‑84/‑295) | yhgE ← / → pck | DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP] |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,532,516 | 1 | . | A | 98.2% | 195.0 / ‑3.7 | 56 | intergenic (‑77/‑302) | yhgE/pck | DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP] |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (26/29); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
ATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCAC > NC_000913/3532385‑3532646 | aTCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑aaa‑aaaa > 1:1026368/1‑139 (MQ=255)aTCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑aaa‑aaaa < 1:1275394/139‑1 (MQ=255) cGGTAGCAATCATCCCCCAGCGTTTTGCGGGTGAAAGTTCAACGTTATCCATGTTAGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑aaa‑aaaaagt < 1:1086186/139‑4 (MQ=255) gTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑aaa‑aaaaagtta > 2:142483/1‑134 (MQ=255) tAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAg < 2:49034/139‑1 (MQ=255) tAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAg > 1:1318292/1‑139 (MQ=255) atcatcCCCCAGCGTGTTGCGGATGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCgtgg < 2:845411/139‑1 (MQ=255) tcatcCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCgtggt < 1:553553/139‑1 (MQ=255) cccccAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAAt < 2:1067773/139‑1 (MQ=255) cccccAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑aaa‑aaaaagt < 2:492652/126‑4 (MQ=255) cccccAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑aaa‑aaaaagt > 1:492652/1‑123 (MQ=255) cccAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCg > 2:821570/1‑139 (MQ=255) ccAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGa > 2:464711/1‑139 (MQ=255) cAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGAt < 2:1318292/139‑1 (MQ=255) gtTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGGAGATAAACAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTa < 1:1176251/139‑1 (MQ=255) gtTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTa > 2:1176251/1‑139 (MQ=255) gTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTg > 2:1259023/1‑139 (MQ=255) cAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCAt < 2:356603/139‑1 (MQ=255) cAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCAt < 2:615910/139‑1 (MQ=255) tCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATttca > 1:274991/1‑139 (MQ=255) tCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATttca > 1:874450/1‑139 (MQ=255) gTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAAtt < 2:158518/131‑1 (MQ=255) gTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAAtt > 1:158518/1‑131 (MQ=255) ttGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAgg > 1:1031389/1‑139 (MQ=255) aTCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCg > 1:112393/1‑139 (MQ=255) gAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTcc < 2:759754/139‑1 (MQ=255) aTCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCAc < 2:960812/139‑1 (MQ=255) tCAAAAGGTTGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt < 1:649352/139‑1 (MQ=255) tCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt < 1:1259023/139‑1 (MQ=255) tCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt < 1:946771/139‑1 (MQ=255) aaaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTcac > 2:480502/1‑139 (MQ=255) ggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGt < 2:828099/90‑1 (MQ=255) ggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGt > 1:828099/1‑90 (MQ=255) ggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTc > 2:1107983/1‑139 (MQ=255) ttAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTccc < 1:464711/139‑1 (MQ=255) ttATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCa > 2:769275/1‑139 (MQ=255) cGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGaa < 1:950108/107‑1 (MQ=255) cGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGaa > 2:950108/1‑107 (MQ=255) cATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt < 2:1237692/117‑1 (MQ=255) cATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt > 1:1237692/1‑117 (MQ=255) tCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAAct < 1:821570/139‑2 (MQ=255) tCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAAcc < 2:274991/139‑1 (MQ=255) tCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAAcc > 2:372421/1‑139 (MQ=255) ccGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAAt < 1:682352/60‑1 (MQ=255) ccGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAAt > 2:682352/1‑60 (MQ=255) ccGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCa < 1:1107983/139‑1 (MQ=255) ccGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCa > 1:1234430/1‑139 (MQ=255) gtaTTTTGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAAcc > 2:347578/1‑139 (MQ=255) ttttGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAttt > 1:129224/1‑132 (MQ=255) ttttGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAttt < 2:129224/132‑1 (MQ=255) ttttGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCttt > 1:842397/1‑139 (MQ=255) tGCTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATGTGCATCAATttcatt < 1:878530/80‑1 (MQ=255) tGCTTTTTTCAGAAAATAAGCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATttcatt > 2:878530/1‑80 (MQ=255) cTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAAcc > 2:765380/1‑120 (MQ=255) cTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAAcc < 1:765380/120‑1 (MQ=255) cTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTgaga < 1:142483/139‑1 (MQ=255) cTTTTTTCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTgaga > 2:1064862/1‑139 (MQ=255) cTTTTTTCAGAAAATAATC‑AAAGAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTgaga > 1:525938/1‑139 (MQ=255) tttttCAGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAAc > 2:1075828/1‑139 (MQ=255) aGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTc < 2:112393/139‑1 (MQ=255) aGAAAATAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTc < 2:842397/139‑1 (MQ=255) aaaTAATCAAAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCcac < 1:372421/139‑1 (MQ=255) | ATCCGGTAGCAATCATCCCCCAGCGTGTTGCGGGTGAAAGTTCAACGTTATCCATGTTGGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAA‑AAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCAC > NC_000913/3532385‑3532646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |