| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,366,415 | A→G | Y163C (TAC→TGC) | arnB → | uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,366,415 | 0 | A | G | 98.2% | 198.2 / NA | 54 | Y163C (TAC→TGC) | arnB | uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/30); minor base C (1/0); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGC > NC_000913/2366280‑2366543 | ttACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCaa < 1:1078032/139‑1 (MQ=255)ttACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCaa < 1:268974/139‑1 (MQ=255) aCGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGa > 2:1111513/1‑139 (MQ=255) atcatTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGGTATTGCAAAGGGCGACATATTGGCGCa < 1:845506/139‑1 (MQ=255) tcatTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCaaa > 1:1304292/1‑139 (MQ=255) tcatTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGCTAC‑GTATTGCAAAGGGCGACATATTGgcg > 2:933786/1‑135 (MQ=255) tcatTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGCTAC‑GTATTGCAAAGGGCGACATATTGgcg < 1:933786/135‑1 (MQ=255) tGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGc > 2:1107535/1‑139 (MQ=255) cGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTtttca > 2:436431/1‑139 (MQ=255) gTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTtttcatt > 1:728164/1‑139 (MQ=255) gTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTtttcatt > 1:377177/1‑139 (MQ=255) tGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTtttcattt > 1:131191/1‑139 (MQ=255) cgcCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTtttcatttca < 1:1111513/139‑1 (MQ=255) ccAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATg > 1:825071/1‑139 (MQ=255) ccAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATg < 1:571144/139‑1 (MQ=255) agATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCAtt < 2:994659/139‑1 (MQ=255) aCGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAatat > 2:1021201/1‑139 (MQ=255) ccATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTAc < 1:1092984/139‑1 (MQ=255) cATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTAcc > 2:983668/1‑139 (MQ=255) aTTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTCTCGAAGATGCCGCCCATGGCGTCGGTAC‑GTATTCCTAAGGGCGACGTATTGGCGCAAGAGGCACAGATATTTTTGACGTTCATGCCGAAAAAAATAGTACCt > 1:680822/1‑139 (MQ=255) ttCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCtg > 1:132501/1‑139 (MQ=255) gcgcCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGc < 1:436431/139‑1 (MQ=255) cgcCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCt > 1:780690/1‑139 (MQ=255) cGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGc < 1:1001553/139‑1 (MQ=255) cGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGc < 2:470276/139‑1 (MQ=255) cGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGc < 2:728164/139‑1 (MQ=255) aaCGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCt < 2:780690/139‑1 (MQ=255) aTCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTg < 2:377177/139‑1 (MQ=255) tCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAatat > 2:796529/1‑106 (MQ=255) tCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAatat < 1:796529/106‑1 (MQ=255) tCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGa < 1:1021201/139‑1 (MQ=255) cGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGAt < 1:849740/139‑1 (MQ=255) gCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATa > 2:853053/1‑139 (MQ=255) cAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGgcg > 2:777475/1‑60 (MQ=255) cAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGgcg < 1:777475/60‑1 (MQ=255) cAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGc < 2:124649/73‑1 (MQ=255) cAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGc > 1:124649/1‑73 (MQ=255) tATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACGGATAATGaa > 2:1062401/1‑139 (MQ=255) aTCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAgg < 2:981417/113‑1 (MQ=255) aTCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAgg > 1:981417/1‑113 (MQ=255) tCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGaaaa < 2:731806/139‑1 (MQ=255) tgctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGcc < 2:1304292/139‑1 (MQ=255) tgctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGcc > 2:1077066/1‑139 (MQ=255) gctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGccc < 2:574942/139‑1 (MQ=255) ctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGgc > 1:783222/1‑44 (MQ=255) ctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGgc < 2:783222/44‑1 (MQ=255) ctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTa > 1:1025129/1‑81 (MQ=255) ctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTa < 2:1025129/81‑1 (MQ=255) ctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCg < 1:1062401/139‑1 (MQ=255) ctgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCg < 2:825071/139‑1 (MQ=255) tgcCCATGCCGTCGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGc < 1:1077066/139‑1 (MQ=255) cGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTAc < 2:658012/78‑1 (MQ=255) cGGTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTAc > 1:658012/1‑78 (MQ=255) ggTAC‑GTATTGCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGc < 1:1107535/139‑1 (MQ=255) | TTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTAC‑GTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGC > NC_000913/2366280‑2366543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |