| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,304,862 | G→A | A319V (GCT→GTT) | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,304,862 | 0 | G | A | 95.7% | 70.0 / ‑6.0 | 23 | A319V (GCT→GTT) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/8); minor base C (1/0); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAGCCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCA > NC_000913/3304728‑3304988 | ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCg > 2:449307/1‑139 (MQ=255)ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCg > 1:7138/1‑139 (MQ=255) aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAg < 1:328627/138‑1 (MQ=255) aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAg > 2:328627/1‑138 (MQ=255) gCTAAACCCGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACACCAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGa < 2:374926/139‑1 (MQ=255) gCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGa < 2:7138/139‑1 (MQ=255) ggCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGt > 1:16616/1‑139 (MQ=255) aGGAATCCGTTCGGGAACAACAG‑CCCCCCCCCCACTGGCCGGGCAAAGGGCAGCCATGCCGGTTTCAAGCCGCCCCAAACCCGAG‑CGGCGAAACCAAACCAGACTTCCAAGTTAG‑CAAACAAAccccaccgttagccga > 2:637648/1‑126 (MQ=255) tCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCTATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaa > 2:123370/1‑139 (MQ=255) cGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacg > 1:447075/1‑139 (MQ=255) cagccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGc < 2:421372/135‑1 (MQ=255) cgccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCa < 1:637648/136‑1 (MQ=255) cccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTg > 2:257962/1‑139 (MQ=255) aCTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAaca > 2:513546/1‑139 (MQ=255) gCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACg > 2:361783/1‑137 (MQ=255) gCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACg < 1:361783/137‑1 (MQ=255) gCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGAc > 2:550014/1‑139 (MQ=255) gCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCg > 2:363769/1‑115 (MQ=255) gCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCg < 1:363769/115‑1 (MQ=255) cAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACcagcag < 1:329114/139‑1 (MQ=255) aGCAATGCCGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGGCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCa > 1:433047/1‑139 (MQ=255) ccGTTTTCAAG‑CGGCTCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGTACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACg > 1:439253/1‑139 (MQ=255) ccGTTTTCAAG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACg > 2:39722/1‑139 (MQ=255) aaG‑CGGCCCACAACCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCa > 1:112617/1‑139 (MQ=255) | CCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAG‑CGGCCCACAGCCGAGTCGTCGAAACCAAA‑CAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCA > NC_000913/3304728‑3304988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |