| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 281,050 | (G)5→6 | coding (934/1155 nt) | yagA ← | CP4‑6 prophage, putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 281,045 | 1 | . | G | 98.0% | 186.1 / ‑3.6 | 51 | coding (939/1155 nt) | yagA | CP4‑6 prophage, putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (23/27); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑C‑GGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGT > NC_000913/280911‑281177 | cTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑C‑gggg > 1:77718/1‑139 (MQ=255) gCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑C‑gggggg > 1:1413589/1‑138 (MQ=255) ccTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTgtgt < 1:859167/139‑1 (MQ=255) gCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGc < 1:335494/139‑1 (MQ=255) aTCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCg < 1:942767/139‑1 (MQ=255) cGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCcgcg < 1:550205/139‑1 (MQ=255) aCCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCt > 2:827574/1‑139 (MQ=255) aCCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCt > 1:210608/1‑139 (MQ=255) aCCCGTTCCCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCt > 1:980901/1‑139 (MQ=255) ttcGCCTTTCCTGCGCTCACACTTACCCCTTTCACGCTCATCTTCCCGCTGATCTCATCTTTCTTAACCATCACCCCTTCACCGTATTCTG‑GGGGGCG‑TTGTGCTGCCCCTGTACTGCCGCGCTGACGGCTGATACcgc < 2:76279/137‑1 (MQ=255) gCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAg < 2:77718/139‑1 (MQ=255) gCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAg < 2:607428/139‑1 (MQ=255) cTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGTTGATATCCACTTTCCTGACCATCACCCCTTCTTCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGcc < 2:980901/139‑1 (MQ=255) cTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTa < 2:696712/139‑1 (MQ=255) cTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTa < 1:867630/139‑1 (MQ=255) cgcTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCg > 2:964355/1‑139 (MQ=255) aGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTgtgt < 1:141/86‑1 (MQ=255) aGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTgtgt > 2:141/1‑86 (MQ=255) aGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCata > 1:1101545/1‑139 (MQ=255) cTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATcc < 2:1101545/139‑1 (MQ=255) ccccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcg < 1:1140371/139‑1 (MQ=255) ttCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCg > 1:778787/1‑139 (MQ=255) ttCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCg > 1:273792/1‑139 (MQ=255) cGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCg > 1:1199452/1‑139 (MQ=255) gCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCgg > 2:550441/1‑139 (MQ=255) cTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCggg > 2:694401/1‑139 (MQ=255) aGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGt > 2:1341100/1‑139 (MQ=255) tttCCGCTGATATCCACTTTCCTGACCGTCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa < 2:1177171/139‑1 (MQ=255) tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa < 1:1228624/139‑1 (MQ=255) tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa < 2:1413589/139‑1 (MQ=255) tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa < 2:1199452/139‑1 (MQ=255) tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa < 2:1242807/139‑1 (MQ=255) tCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGtt < 1:783100/135‑1 (MQ=255) tCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGtt > 2:783100/1‑135 (MQ=255) atatCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAg > 2:1369069/1‑139 (MQ=255) atatCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAg > 1:745366/1‑139 (MQ=255) tatCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGa < 2:778787/139‑1 (MQ=255) ccTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTg < 2:1380310/109‑1 (MQ=255) ccTGACCATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTg > 1:1380310/1‑109 (MQ=255) cATCACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa > 1:970217/1‑113 (MQ=255) cATCACCCCTTCATCGTATCC‑CGGGGGGCG‑TTGTGTTACCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGATCa < 2:970217/113‑1 (MQ=255) cgccccTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGaa > 2:740775/3‑139 (MQ=255) cACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGaa > 1:1491778/1‑139 (MQ=255) cACCCCTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGaa > 2:54894/1‑139 (MQ=255) ccTTCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGgcg < 1:827574/139‑1 (MQ=255) ttCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGgcgcg < 1:1341100/139‑1 (MQ=255) tCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa > 1:1061557/1‑103 (MQ=255) tCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa < 2:1061557/103‑1 (MQ=255) tCATCGTATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGgcgcgc < 1:54894/139‑1 (MQ=255) ttctatttC‑C‑GGGGGGGTTTGTTTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGAGAGCCCGGTTCACCCATATCCAGCGCTTCGTGCGGGCGTTCAAGGTTATAGGCCGTCCGCCAGTTGTCGAAGGCGCGCTg < 2:761869/133‑1 (MQ=255) tATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa > 2:784930/1‑97 (MQ=255) tATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa < 1:784930/97‑1 (MQ=255) tATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGt < 1:1201459/139‑1 (MQ=255) tATTC‑CGGGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGt > 2:1178332/1‑139 (MQ=255) | CTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTC‑C‑GGGGGCG‑TTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGT > NC_000913/280911‑281177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |