| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,330 | (G)8→9 | noncoding (72/87 nt) | leuQ ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,322 | 1 | . | G | 95.8% | 157.7 / 0.2 | 48 | noncoding (80/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (25/21); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-01 | |||||||||||
TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGT > NC_000913/4606184‑4606453 | ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑Ga > 2:822210/1‑139 (MQ=255) cGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑ggggggg > 1:1322828/1‑139 (MQ=255) gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAAcccc > 1:1484784/1‑139 (MQ=255) ggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTc < 1:822210/139‑1 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGt > 2:1132787/1‑139 (MQ=255) gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTaa < 1:195600/139‑1 (MQ=255) cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGTATTGGACCCCCACGCCCGTCAgg > 2:1040750/1‑139 (MQ=21) ttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTa > 2:389187/1‑135 (MQ=255) ttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTa < 1:389187/135‑1 (MQ=255) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGaa < 1:831406/119‑1 (MQ=255) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGaa > 2:831406/1‑119 (MQ=255) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCa > 2:1369569/1‑125 (MQ=255) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCa < 1:1369569/125‑1 (MQ=255) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGaca > 1:399502/1‑139 (MQ=255) aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt < 1:1043206/139‑1 (MQ=255) aGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 1:1132787/139‑1 (MQ=255) acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc > 1:681621/1‑139 (MQ=255) acacCAACACCTGAAGCTAGAATGTGAACCAATTCTGAAACCTCAGGCCAGTACCAACAATGGCAACATGGTTTTTGGTGCGGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc < 2:1085303/139‑1 (MQ=9) acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa < 1:1219985/139‑1 (MQ=255) gCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGGGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:681621/139‑1 (MQ=255) tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa > 2:1381349/1‑139 (MQ=255) aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc > 2:1205725/1‑139 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 1:310338/1‑139 (MQ=255) tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt < 2:1322828/139‑1 (MQ=255) tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt < 1:1205725/139‑1 (MQ=255) aTTCCGCCACGTCCGCCCAGTGCGAGCAAAATCAACGGGGTTTTTTGGGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt < 2:1484784/139‑1 (MQ=18) tCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gggggg < 2:1481345/56‑1 (MQ=255) tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAAccccc < 2:973508/71‑1 (MQ=255) tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAAccccc > 1:973508/1‑71 (MQ=255) tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gggggg > 1:1481345/1‑56 (MQ=255) cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt < 2:51114/106‑1 (MQ=255) cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt > 1:51114/1‑106 (MQ=255) gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt > 2:1315492/1‑139 (MQ=255) ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc < 2:708934/120‑1 (MQ=255) ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAAAACCTGAAGCTAGCGCTTCTACCAATTCCGCCAc > 1:708934/1‑120 (MQ=21) tGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGc > 2:775301/1‑139 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gggg > 1:793828/1‑36 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gggg < 2:793828/36‑1 (MQ=255) gAGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc > 2:761378/1‑139 (MQ=255) aGCAATATCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc > 1:507844/1‑139 (MQ=255) atatCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACgg < 1:775301/139‑1 (MQ=255) atCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCa > 1:1082822/1‑44 (MQ=18) atCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCa < 2:1082822/44‑1 (MQ=18) atCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg > 1:1219776/1‑139 (MQ=255) atCAACGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg < 1:1040750/139‑1 (MQ=255) aCGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 2:1244849/138‑1 (MQ=255) aCGTGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg > 1:1244849/1‑138 (MQ=255) tGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTa > 2:439216/1‑45 (MQ=14) tGGTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTa < 1:439216/45‑1 (MQ=14) gTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt > 2:751311/1‑139 (MQ=255) gTTTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt > 2:1200219/1‑139 (MQ=255) ttttGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg < 1:751311/139‑1 (MQ=255) tttGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa > 2:723246/1‑82 (MQ=31) tttGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa < 1:723246/82‑1 (MQ=31) ggTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGTCACTAACAACTGAAGCTAACGCGTCTACCAATGCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCAGTCCAGTgga > 2:181707/1‑138 (MQ=21) ggTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg > 2:1185897/1‑124 (MQ=255) ggTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg < 1:1185897/124‑1 (MQ=255) | TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGT > NC_000913/4606184‑4606453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |