| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,756,910 | (A)6→5 | coding (1213/1413 nt) | pykF → | pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,756,905 | 0 | A | . | 100.0% | 147.2 / NA | 35 | coding (1208/1413 nt) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (20/15); total (20/15) | |||||||||||
TGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGG > NC_000913/1756772‑1757034 | tGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGTCACCATCCTGGCACTGACCACCAACGaaaaac > 2:446453/1‑138 (MQ=255) ggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGTCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca < 1:446453/139‑1 (MQ=255) tgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtcatc < 2:305948/139‑1 (MQ=255) aCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACt > 2:73248/1‑139 (MQ=255) tCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGtt < 2:250553/139‑1 (MQ=255) ggTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAag > 1:897555/1‑139 (MQ=255) aCTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAc > 1:370899/1‑139 (MQ=255) aGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag < 1:147396/130‑1 (MQ=255) aGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag > 2:147396/1‑130 (MQ=255) cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTc < 1:73248/139‑1 (MQ=255) cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAGAACGTCTCATCAGTTGGTACTGAGCAAAGGCg < 1:542950/73‑1 (MQ=255) cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCg > 2:542950/1‑73 (MQ=255) tCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGc > 2:873847/1‑83 (MQ=255) tCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGc < 1:873847/83‑1 (MQ=255) tCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTaaa > 2:298268/1‑91 (MQ=255) tCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTaaa < 1:298268/91‑1 (MQ=255) tCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAc < 2:904580/99‑1 (MQ=255) tCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAc > 1:904580/1‑99 (MQ=255) cccGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca > 2:444993/1‑47 (MQ=39) cccGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca < 1:444993/47‑1 (MQ=39) cccGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctc > 2:371925/1‑139 (MQ=255) ggCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa > 1:391081/1‑62 (MQ=255) ggCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa < 2:391081/62‑1 (MQ=255) cACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGcacac < 1:378199/139‑1 (MQ=255) tGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACaaa > 2:196144/1‑139 (MQ=255) gACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAg > 1:770268/1‑139 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag < 2:488615/68‑1 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag > 1:488615/1‑68 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAt < 1:23914/70‑1 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAt > 2:23914/1‑70 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCt > 1:53254/1‑90 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCt < 2:53254/90‑1 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg > 2:62307/1‑139 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg > 1:833231/1‑139 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg > 1:351842/1‑139 (MQ=255) accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACAGTCTGGGTAAAGAACTGGCTCTGAAGAGCGGTCTGGCACACAAAgg > 1:903762/1‑139 (MQ=255) | TGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGG > NC_000913/1756772‑1757034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |