Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,902,316	(T)8→7	coding (1386/1413 nt)	bglB ←	cryptic phospho‑beta‑glucosidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,902,309	0	T	.	100.0%	170.8 / NA	40	coding (1393/1413 nt)	bglB	cryptic phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/23);  total (17/23)

CATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA  >  NC_000913/3902179‑3902436                                                                                                                                   |                                                                                                                                cATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTAtttttttaa                                                                                                                         >  2:231698/1‑137 (MQ=255)            tGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGccccc                                                                                                             <  2:343308/139‑1 (MQ=255)             ggCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCcg                                                                                                            >  1:99852/1‑139 (MQ=255)               cAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCcgcg                                                                                                          <  2:299167/139‑1 (MQ=255)               cAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCcgcg                                                                                                          >  1:898118/1‑139 (MQ=255)                        taaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCa                                                                                                 <  2:854264/139‑1 (MQ=255)                         aaGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCAc                                                                                                <  1:231698/139‑1 (MQ=255)                          aGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCAcc                                                                                               <  1:682710/139‑1 (MQ=255)                               tCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGc                                                                                          >  2:386022/1‑139 (MQ=255)                                   cTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATAc                                                                                      <  1:884746/139‑1 (MQ=255)                                          taataTCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGa                                                                               <  2:898118/139‑1 (MQ=255)                                             tatCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTAtt                                                                                                                                >  2:848746/1‑88 (MQ=255)                                             tatCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTAtt                                                                                                                                <  1:848746/88‑1 (MQ=255)                                                     tATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTCATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCtttt                                                                        <  1:435935/135‑1 (MQ=255)                                                     tATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTCATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCtttt                                                                        >  2:435935/1‑135 (MQ=255)                                                      aTGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGc                                                                   <  2:223178/139‑1 (MQ=255)                                                          tGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGTGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGAc                                                                                                                    >  1:646409/1‑86 (MQ=255)                                                          tGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGAc                                                                                                                    <  2:646409/86‑1 (MQ=255)                                                          tGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCgtgt                                                               >  1:353015/1‑139 (MQ=255)                                                            cAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTAttttttta                                                                                                                          <  1:297864/79‑2 (MQ=255)                                                            cAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTAttttttta                                                                                                                          >  2:297864/1‑78 (MQ=255)                                                            cAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTc                                                             >  2:477545/1‑139 (MQ=255)                                                               agaaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                                          >  1:115609/1‑139 (MQ=255)                                                                 aaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGa                                                                               >  1:586104/1‑116 (MQ=255)                                                                 aaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGa                                                                               <  2:586104/116‑1 (MQ=255)                                                                           cgcatcgcatCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                                              <  2:99852/139‑1 (MQ=255)                                                                              atcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGc                                           >  2:807970/1‑139 (MQ=255)                                                                               tcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGcc                                          >  1:751466/1‑139 (MQ=255)                                                                                        gACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatcatc                                 <  1:104647/139‑1 (MQ=255)                                                                                        gACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatcatc                                 <  1:807970/139‑1 (MQ=255)                                                                                            gTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGa                             <  1:477545/139‑1 (MQ=255)                                                                                                 gAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                        <  2:353015/139‑1 (MQ=255)                                                                                                 gAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                        <  1:386022/139‑1 (MQ=255)                                                                                                  aaTGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcaca                       <  2:174659/139‑1 (MQ=255)                                                                                                       gacgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAgg                                                     >  2:157634/1‑104 (MQ=255)                                                                                                       gacgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAgg                                                     <  1:157634/104‑1 (MQ=255)                                                                                                            tAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAtcatca                                <  2:430409/120‑1 (MQ=255)                                                                                                            tAATTAAGGTGCTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAtcatca                                >  1:430409/1‑120 (MQ=255)                                                                                                                       cTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAgg                                                     <  2:237408/88‑1 (MQ=255)                                                                                                                       cTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAgg                                                     >  1:237408/1‑88 (MQ=255)                                                                                                                       cTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATc                           >  2:443960/1‑114 (MQ=255)                                                                                                                       cTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATc                           <  1:443960/114‑1 (MQ=255)                                                                                                                       cTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCtt   >  1:2538/1‑138 (MQ=255)                                                                                                                       cTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCtt   <  2:2538/138‑1 (MQ=255)                                                                                                                       cTTTAATGGTTA‑TTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTa  >  2:899104/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                CATTAATAAGATGGCAGAGGTAATAAGATTTCGTCTAAACATAATATCCCTTTATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTA  >  NC_000913/3902179‑3902436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑