Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	752,402	(T)5→4	coding (394/567 nt)	ybgD ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	752,398	0	T	.	100.0%	165.1 / NA	39	coding (398/567 nt)	ybgD	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/19);  total (20/19)

TACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCG  >  NC_000913/752265‑752532                                                                                                                                      |                                                                                                                                       tACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCAtt                                                                                                                                       <  2:285360/135‑1 (MQ=255) tACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCAtt                                                                                                                                       >  1:285360/1‑135 (MQ=255)     tCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCAt                                                                                                                              <  1:124738/139‑1 (MQ=255)                         tGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAg                                                                                                          >  1:687010/1‑139 (MQ=255)                                     ttGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTg                                                                                              >  2:680304/1‑139 (MQ=255)                                          ccatccaGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGTTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGGCCATCAGTCGTACACCGACCCCAGTTGCTTCGcc                                                                                                <  1:685705/132‑1 (MQ=255)                                          ccatccaGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGcc                                                                                                >  2:685705/1‑132 (MQ=255)                                             tccaGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGgtg                                                                                      >  1:380302/1‑139 (MQ=255)                                                  gCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGAc                                                                                                               <  2:396897/109‑1 (MQ=255)                                                  gCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGAc                                                                                                               >  1:396897/1‑109 (MQ=255)                                                       aaaGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTacac                                                                                                                   >  1:869778/1‑100 (MQ=255)                                                       aaaGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTacac                                                                                                                   <  2:869778/100‑1 (MQ=255)                                                             cAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCg                                                                      >  2:430035/1‑139 (MQ=255)                                                                     gTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCa                                                              <  1:680304/139‑1 (MQ=255)                                                                                cTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTaca                                                                                                                    >  1:236049/1‑74 (MQ=255)                                                                                cTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTaca                                                                                                                    <  2:236049/74‑1 (MQ=255)                                                                                cTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGgtg                                                                                      >  1:152337/1‑104 (MQ=255)                                                                                cTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGgtg                                                                                      <  2:152337/104‑1 (MQ=255)                                                                                cTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCa                                                              <  1:497314/128‑1 (MQ=255)                                                                                cTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCa                                                              >  2:497314/1‑128 (MQ=255)                                                                                cTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGc                                                   >  1:617556/1‑139 (MQ=255)                                                                                  tGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTg                                                                                   <  2:685855/105‑1 (MQ=255)                                                                                  tGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTg                                                                                   >  1:685855/1‑105 (MQ=255)                                                                                   gCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGc                                                                                                      <  2:409519/85‑1 (MQ=255)                                                                                   gCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGc                                                                                                      >  1:409519/1‑85 (MQ=255)                                                                                    cATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTg                                               <  2:507439/139‑1 (MQ=255)                                                                                          ggTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCg                                         <  1:430035/139‑1 (MQ=255)                                                                                              aaGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGt                                                                     >  2:23614/1‑107 (MQ=255)                                                                                              aaGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGt                                                                     <  1:23614/107‑1 (MQ=255)                                                                                               aGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAgg                                                                        <  2:257656/103‑1 (MQ=255)                                                                                               aGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAgg                                                                        >  1:257656/1‑103 (MQ=255)                                                                                                     ggCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCa                                                              >  2:236936/1‑107 (MQ=255)                                                                                                     ggCGCGGCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCa                                                              <  1:236936/107‑1 (MQ=255)                                                                                                           gCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCAt                                                                                                                              >  2:347185/1‑37 (MQ=255)                                                                                                           gCTGAACCTAATACGATGTTACCGTCA‑TTTTGTCCAt                                                                                                                              <  1:347185/37‑1 (MQ=255)                                                                                                              gAACCTAATACGATGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGa                     <  1:97043/139‑1 (MQ=255)                                                                                                                           tGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCAc                                                                                           <  2:135646/56‑1 (MQ=255)                                                                                                                           tGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCAc                                                                                           >  1:135646/1‑56 (MQ=255)                                                                                                                           tGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCg        >  2:397656/1‑139 (MQ=255)                                                                                                                           tGTTACCGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTG‑‑TCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCt      >  2:467585/1‑139 (MQ=255)                                                                                                                                 cGTCA‑TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCg  <  1:397656/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCG  >  NC_000913/752265‑752532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑