| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,763,869 | (T)7→8 | coding (1285/1617 nt) | yfjL ← | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,763,862 | 1 | . | T | 97.9% | 155.8 / ‑3.6 | 47 | coding (1292/1617 nt) | yfjL | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (24/22); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG > NC_000913/2763737‑2763995 | tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg > 1:508769/1‑139 (MQ=255) agagTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcgc < 1:258614/139‑1 (MQ=255) tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTa > 2:260168/1‑139 (MQ=255) tAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGGCTTTCATGTCGAGGATTTT‑TTTTGCGCGCGGTATCg > 2:545547/1‑139 (MQ=255) gCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGAt > 2:219095/1‑139 (MQ=255) aacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttt‑tttttg > 2:82502/1‑111 (MQ=255) aacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttt‑tttttg < 1:82502/113‑3 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:219095/139‑1 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg > 2:717082/1‑107 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg < 1:717082/107‑1 (MQ=255) gATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACt < 2:508769/139‑1 (MQ=255) aTCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:757338/128‑1 (MQ=255) aTCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 1:757338/1‑128 (MQ=255) aGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg < 2:60258/98‑1 (MQ=255) aGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg > 1:60258/1‑98 (MQ=255) aGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg > 1:775195/1‑93 (MQ=255) aGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAt > 2:318389/1‑139 (MQ=255) aGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCAAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg < 2:775195/93‑1 (MQ=255) aGCATCGAAAGGTTGGCTACCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 2:495640/1‑119 (MQ=255) aGCATCGAAAGGTTGGCTACCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:495640/119‑1 (MQ=255) gAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAt > 2:242885/1‑133 (MQ=255) gAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAt < 1:242885/133‑1 (MQ=255) aaaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTAt < 2:654807/92‑1 (MQ=255) aaaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTATTTTGCGCGCGGTAt > 1:654807/1‑92 (MQ=255) ggCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGa > 1:574948/1‑139 (MQ=255) gCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGAc > 2:507347/1‑139 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 1:318389/139‑1 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttt‑tttttgcg < 1:264488/73‑5 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttt‑tttttgcg > 2:264488/1‑69 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:607228/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:725703/139‑1 (MQ=255) gAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCTATCTGACGGCAATGTTaa < 1:507347/139‑1 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCg > 1:379517/1‑90 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCg < 2:379517/90‑1 (MQ=255) tCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATccc > 2:274854/1‑101 (MQ=255) tCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATccc < 1:274854/101‑1 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCg > 1:30028/1‑139 (MQ=255) aTCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGtt < 2:445523/103‑1 (MQ=255) aTCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGtt > 1:445523/1‑103 (MQ=255) tCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGt > 2:429119/1‑111 (MQ=255) tCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGt < 1:429119/111‑1 (MQ=255) tCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa > 2:484135/1‑139 (MQ=255) cTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGt > 2:174329/1‑52 (MQ=39) cTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGt < 1:174329/52‑1 (MQ=39) tttAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGaa > 2:331836/1‑47 (MQ=38) tttAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGaa < 1:331836/47‑1 (MQ=38) tttAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAAc > 1:735956/1‑113 (MQ=255) tttAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAAc < 2:735956/113‑1 (MQ=255) tttAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATc < 1:484135/139‑1 (MQ=255) tAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACAAACTCGCTGAAGCAAATCCAAAGCTGATCAc > 2:90722/1‑139 (MQ=255) gAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTgg > 2:715769/1‑139 (MQ=255) | TAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG > NC_000913/2763737‑2763995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |