| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 34,120 | (T)9→8 | intergenic (+82/‑180) | carB → / → caiF | carbamoyl‑phosphate synthase large subunit/cai operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 34,112 | 0 | T | . | 94.4% | 206.8 / 6.1 | 54 | intergenic (+74/‑188) | carB/caiF | carbamoyl‑phosphate synthase large subunit/cai operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base . (29/22); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGG > NC_000913/33976‑34236 | cTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttt > 1:48433/1‑139 (MQ=255) cGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTa > 2:37389/1‑139 (MQ=255) aaTTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTg > 1:217991/1‑139 (MQ=255) gATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAg < 1:37389/139‑1 (MQ=255) aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGc > 2:243417/1‑139 (MQ=255) aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGc > 2:388402/1‑139 (MQ=255) aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGc > 1:47558/1‑139 (MQ=255) cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGata < 2:432438/110‑1 (MQ=255) cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGata > 1:432438/1‑110 (MQ=255) cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa > 2:436229/1‑128 (MQ=255) cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa < 1:436229/128‑1 (MQ=255) cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTc > 2:581488/1‑139 (MQ=255) tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCa < 1:69741/81‑1 (MQ=255) tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCa > 2:69741/1‑81 (MQ=255) tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGAc > 1:15981/1‑119 (MQ=255) tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGAc < 2:15981/119‑1 (MQ=255) tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcac > 2:245252/1‑139 (MQ=255) tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcac > 2:164064/1‑139 (MQ=255) tCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCacac > 1:513699/1‑139 (MQ=255) atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACg > 2:617036/1‑139 (MQ=255) aTCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTg < 2:435196/139‑1 (MQ=255) tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt < 1:313485/110‑1 (MQ=255) tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt > 2:313485/1‑110 (MQ=255) ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa > 1:361838/1‑139 (MQ=255) ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa < 1:581488/139‑1 (MQ=255) ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa < 1:617036/139‑1 (MQ=255) ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa < 2:349111/139‑1 (MQ=255) gCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATa > 1:414972/1‑139 (MQ=255) gCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATa < 2:361838/139‑1 (MQ=255) tttGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATc < 2:422872/139‑1 (MQ=255) gATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGat < 2:217991/139‑1 (MQ=255) tCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg < 2:276638/122‑1 (MQ=255) tCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg > 1:276638/1‑122 (MQ=255) cGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca > 1:216005/1‑90 (MQ=255) cGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca < 2:216005/90‑1 (MQ=255) ttCTCTGCCGACGACCGTGC‑TTTTTCCACATTAAGTAAATCATTTGGGGAAACGATATTTTTGATGTCATAAGCAGGCATTAGATCACGCCTATCGACGGTGAAGTTGCATACTATGTATATATCCACACTTTTAatat > 1:472008/1‑139 (MQ=255) ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACt < 1:372819/114‑1 (MQ=255) ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACt > 2:372819/1‑114 (MQ=255) ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAatat > 2:240457/1‑139 (MQ=255) ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAatat > 2:23940/1‑139 (MQ=255) ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAatat > 2:140341/1‑139 (MQ=255) ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAatat > 1:218503/1‑139 (MQ=255) ctctGATGAGGACCG‑TTTTTTTTGCCCATTCAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATgg < 2:47558/139‑1 (MQ=255) ctctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca < 2:498563/85‑1 (MQ=255) ctctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca > 1:498563/1‑85 (MQ=255) ctctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa < 2:1830/108‑1 (MQ=255) ctctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa > 1:1830/1‑108 (MQ=255) aGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt < 2:172635/75‑1 (MQ=255) aGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt > 1:172635/1‑75 (MQ=255) gACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt > 2:509326/1‑73 (MQ=255) gACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt < 1:509326/73‑1 (MQ=255) gACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAata > 1:515303/1‑126 (MQ=255) gACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAata < 2:515303/126‑1 (MQ=255) aCCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAat < 2:466353/125‑1 (MQ=255) aCCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAat > 1:466353/1‑125 (MQ=255) tttttttttGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt > 2:166164/1‑69 (MQ=255) tttttttttGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt < 1:166164/69‑1 (MQ=255) | CTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGG > NC_000913/33976‑34236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |