| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,993,464 | Δ1 bp | coding (2312/2547 nt) | cyaA → | adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,993,464 | 0 | A | . | 89.7% | 90.7 / 8.8 | 30 | coding (2312/2547 nt) | cyaA | adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base . (13/13); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGA > NC_000913/3993326‑3993589 | aTCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTGTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATcacca > 1:370416/1‑139 (MQ=255) ttGCCTGCTAAGGGATCATCCTGTTCTTTTTCGAAGAAACGCAAGACGAGATTGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGAc < 1:611913/139‑1 (MQ=255) tGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACg > 1:399454/1‑139 (MQ=255) gAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCg > 2:97582/1‑139 (MQ=255) gAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCg < 2:399454/139‑1 (MQ=255) taatcctattCTTTCACGAAGAAACACAAGACGAGAATGGCTTTAATATCTACATTCGCGACGAAAGCAACCGGGTAGAGGTAAATCACC‑CTGCGAAGGCAGCAAAGACGAGCTAGTACGTGACGGCAGTCGCTTCTAc < 1:220942/131‑1 (MQ=255) ccAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGt < 1:504690/139‑1 (MQ=255) aGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGTAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCa < 2:421122/139‑1 (MQ=255) ttCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATc > 1:516893/1‑139 (MQ=255) ttCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATg < 2:370416/139‑1 (MQ=255) tCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGa < 2:394023/139‑1 (MQ=255) aCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAgg < 1:293813/76‑1 (MQ=255) aCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAgg > 2:293813/1‑76 (MQ=255) cAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCt < 2:208620/139‑1 (MQ=255) cAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTa < 1:346530/139‑1 (MQ=255) gACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACgg > 1:516162/1‑139 (MQ=255) cGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCt > 1:157375/1‑139 (MQ=255) aaTGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAg > 1:488446/1‑139 (MQ=255) cTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGa < 1:22724/113‑1 (MQ=255) cTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGa > 2:22724/1‑113 (MQ=255) cTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGt < 2:352023/81‑1 (MQ=255) cTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGt > 1:352023/1‑81 (MQ=255) cTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCa > 2:494978/1‑139 (MQ=255) cTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCa > 2:575656/1‑139 (MQ=255) tcGACGAATGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCg > 2:400053/1‑139 (MQ=255) acgaAAGCAACCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCTAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAg < 1:400053/139‑1 (MQ=255) aaCCGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCa < 1:494978/139‑1 (MQ=255) ccGGGTTGAGGTATATCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGa < 2:516893/139‑1 (MQ=255) tGAGGTAAATC‑CCCCTGCGAAGGCAGCAAAGAAGAGCTGGTACGGTACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGa < 2:563064/139‑1 (MQ=255) tatCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCa > 1:459745/1‑89 (MQ=255) tatCACC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCa < 2:459745/89‑1 (MQ=255) | ATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGA > NC_000913/3993326‑3993589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |