| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,351,196 | (G)7→8 | intergenic (‑157/+211) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,351,189 | 1 | . | G | 94.2% | 176.9 / 3.0 | 52 | intergenic (‑150/+218) | fabI/ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (24/25); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
GATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA > NC_000913/1351059‑1351319 | gATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gggggggg > 1:949852/1‑138 (MQ=255) aTGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGgcg > 1:848929/1‑139 (MQ=255) aTGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGgcg > 2:784773/1‑139 (MQ=255) gCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATTAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑ggggggggcgc > 1:1680028/1‑135 (MQ=255) cTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCtt < 1:784773/139‑1 (MQ=255) ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gggggggg > 2:441731/1‑130 (MQ=255) ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gggggggg < 1:441731/131‑2 (MQ=255) cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCctct < 1:586933/139‑1 (MQ=255) gATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct < 2:244251/136‑1 (MQ=255) gATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct > 1:244251/1‑136 (MQ=255) tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCg > 2:1440330/1‑139 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAccc > 1:346840/1‑116 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAccc < 2:346840/116‑1 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt > 2:716631/1‑102 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt > 1:1672930/1‑102 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt < 1:716631/102‑1 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt < 2:1672930/102‑1 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGAGAGGGGACCGt > 2:269457/1‑139 (MQ=255) aCGAACAGATAAACGGTTATTATAATTAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa < 2:1680028/139‑1 (MQ=255) aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa < 2:1281223/139‑1 (MQ=255) cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct < 2:736846/112‑1 (MQ=255) cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct > 1:736846/1‑112 (MQ=255) cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt > 1:816539/1‑139 (MQ=255) ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc > 2:456262/1‑111 (MQ=255) ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc < 1:456262/111‑1 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTcccc > 2:1007428/1‑82 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTcccc < 1:1007428/82‑1 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa > 1:1697731/1‑111 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa < 2:1697731/111‑1 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc > 1:793705/1‑139 (MQ=255) tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt < 1:1640380/76‑1 (MQ=255) tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt > 2:1640380/1‑76 (MQ=255) cAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaa < 2:793705/139‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa > 1:662953/1‑110 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa < 2:662953/110‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCCCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa > 1:245838/1‑139 (MQ=255) cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa < 2:797507/139‑1 (MQ=255) ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACGCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc > 2:1083909/1‑127 (MQ=255) ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc < 1:1083909/127‑1 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt < 1:1232058/68‑1 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt > 2:1232058/1‑68 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt > 2:355425/1‑68 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt < 1:355425/68‑1 (MQ=255) cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg < 1:535415/139‑1 (MQ=255) gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa > 2:311946/1‑120 (MQ=255) gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa < 1:311946/120‑1 (MQ=255) cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAcc < 2:610097/57‑1 (MQ=38) cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAcc > 1:610097/1‑57 (MQ=38) aCCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc > 2:427133/1‑139 (MQ=255) aGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga < 2:667112/55‑1 (MQ=21) aGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga > 1:667112/1‑55 (MQ=21) gCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGc < 1:1444625/139‑1 (MQ=255) gCTGCGGCAGGGGGGGGGCGCTTTTCCCCTCACCCTAAcc > 1:1244139/1‑40 (MQ=18) gCTGCGGCAGGGGGGGGGCGCTTTTCCCCTCACCCTAAcc < 2:1244139/40‑1 (MQ=18) gCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTggg > 2:871422/1‑139 (MQ=255) cTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa < 2:949852/139‑1 (MQ=255) | GATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA > NC_000913/1351059‑1351319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |