Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,935	A→G	L69L (TTG→CTG)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,935	0	A	G	100.0%	379.0 / NA	99	L69L (TTG→CTG)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/55);  total (44/55)

ATCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAG  >  NC_000913/4490802‑4491059                                                                                                                                      |                                                                                                                              aTCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAc                                                                                                                          >  2:1435964/1‑139 (MQ=255)  tCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         <  1:872339/139‑1 (MQ=255)    aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       <  2:317771/139‑1 (MQ=255)    aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       <  2:1025214/139‑1 (MQ=255)    aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       <  2:321182/139‑1 (MQ=255)        aCTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGt                                                                                                                  >  1:891637/1‑139 (MQ=255)            ttcttcGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAGTTATGCCCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGACGAGTTGAGTTTGACATGACGGAATCAGGATACCGA‑GGGTATaa                                                                                                              >  1:240337/1‑139 (MQ=255)             tcttcGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAAc                                                                                                             >  2:1430626/1‑139 (MQ=255)              cttcGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACt                                                                                                            <  2:891637/139‑1 (MQ=255)                  ggAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTtgt                                                                                                        <  2:1573248/139‑1 (MQ=255)                     aTCGCGATCGTCATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCa                                                                                                     <  2:1158893/139‑1 (MQ=255)                      tCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGGATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCAt                                                                                                    >  1:1707557/1‑139 (MQ=255)                      tCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         >  2:414157/1‑119 (MQ=255)                      tCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         <  1:414157/119‑1 (MQ=255)                             cGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTcaacaa                                                                                             <  1:335890/139‑1 (MQ=255)                             cGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTcaacaa                                                                                             <  1:1140101/139‑1 (MQ=255)                                      aattAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCggg                                                                                    >  2:195025/1‑139 (MQ=255)                                        ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCGGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  <  1:1540089/139‑1 (MQ=255)                                          aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         <  1:11618/99‑1 (MQ=255)                                          aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         >  2:11618/1‑99 (MQ=255)                                          aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAgcg                                                                                <  2:758957/139‑1 (MQ=255)                                          aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAgcg                                                                                >  1:115574/1‑139 (MQ=255)                                               tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTcaacaa                                                                                             >  2:961272/1‑121 (MQ=255)                                               tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTcaacaa                                                                                             <  1:961272/121‑1 (MQ=255)                                               tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  2:1758902/139‑1 (MQ=255)                                               tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  1:1430626/139‑1 (MQ=255)                                               tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  1:100060/139‑1 (MQ=255)                                                    gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:232921/139‑1 (MQ=255)                                                    gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:143198/139‑1 (MQ=255)                                                    gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:1004854/139‑1 (MQ=255)                                                    gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:115574/139‑1 (MQ=255)                                                     tCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      >  1:1078934/1‑138 (MQ=255)                                                     tCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:1078934/138‑1 (MQ=255)                                                      cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTtgtg                                                                                                       <  1:1302329/104‑1 (MQ=255)                                                      cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTtgtg                                                                                                       >  2:1302329/1‑104 (MQ=255)                                                       tGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           >  2:821008/1‑131 (MQ=255)                                                       tGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  1:821008/131‑1 (MQ=255)                                                       tGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGatat                                                                   >  1:1069543/1‑139 (MQ=255)                                                       tGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGatat                                                                   >  1:460711/1‑139 (MQ=255)                                                         aTAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         <  1:1645034/84‑1 (MQ=255)                                                         aTAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         >  2:1645034/1‑84 (MQ=255)                                                         aTAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  1:1067787/129‑1 (MQ=255)                                                         aTAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           >  2:1067787/1‑129 (MQ=255)                                                              tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         >  1:1674617/1‑79 (MQ=255)                                                              tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                         <  2:1674617/79‑1 (MQ=255)                                                              tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       <  1:1392859/81‑1 (MQ=255)                                                              tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       >  2:1392859/1‑81 (MQ=255)                                                              tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  <  1:71314/117‑1 (MQ=255)                                                              tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  >  2:71314/1‑117 (MQ=255)                                                              tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                            <  1:943033/139‑1 (MQ=255)                                                               aTGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  1:695366/128‑1 (MQ=255)                                                               aTGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      >  2:695366/1‑128 (MQ=255)                                                                  cTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGta                                                                                                                 <  2:1447151/82‑1 (MQ=255)                                                                  cTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGta                                                                                                                 >  1:1447151/1‑82 (MQ=255)                                                                   tCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  1:1767192/124‑1 (MQ=255)                                                                   tCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      >  2:1767192/1‑124 (MQ=255)                                                                     agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACttt                                                                                                          <  1:660467/86‑1 (MQ=255)                                                                     agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACttt                                                                                                          >  2:660467/1‑86 (MQ=255)                                                                     agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  1:306179/117‑1 (MQ=255)                                                                     agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           >  2:306179/1‑117 (MQ=255)                                                                     agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTcc                                                     >  2:990761/1‑139 (MQ=255)                                                                     agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTcc                                                     >  1:515135/1‑139 (MQ=255)                                                                      gagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCa                                                    <  1:1496845/139‑1 (MQ=255)                                                                       agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                   >  2:1203425/1‑139 (MQ=255)                                                                       agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                   >  2:1362957/1‑139 (MQ=255)                                                                          tGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCAtt                                                                                                   <  2:569485/88‑1 (MQ=255)                                                                          tGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCAtt                                                                                                   >  1:569485/1‑88 (MQ=255)                                                                          tGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAAt                                                >  1:1571410/1‑139 (MQ=255)                                                                            aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  <  2:989723/103‑1 (MQ=255)                                                                            aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  >  1:989723/1‑103 (MQ=255)                                                                            aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAAttt                                              <  1:1736557/139‑1 (MQ=255)                                                                                 ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  <  2:1328262/98‑1 (MQ=255)                                                                                 ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  >  1:1328262/1‑98 (MQ=255)                                                                                 ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAtct                                                          >  2:352979/1‑122 (MQ=255)                                                                                 ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAtct                                                          <  1:352979/122‑1 (MQ=255)                                                                                 ttCTATTCCCACGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       >  2:817668/1‑62 (MQ=255)                                                                                 ttCTATTCCCACGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       <  1:817668/62‑1 (MQ=255)                                                                                   cTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGAt                                       >  1:1776444/1‑139 (MQ=255)                                                                                      ttCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTcaac                                                                                               >  1:1169868/1‑80 (MQ=255)                                                                                      ttCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTcaac                                                                                               <  2:1169868/80‑1 (MQ=255)                                                                                      ttCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTg                                                              >  1:511/1‑113 (MQ=255)                                                                                      ttCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTg                                                              <  2:511/113‑1 (MQ=255)                                                                                         ccGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       >  1:1019185/1‑54 (MQ=255)                                                                                         ccGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                       <  2:1019185/54‑1 (MQ=255)                                                                                          cgcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      >  2:1593089/1‑101 (MQ=255)                                                                                          cgcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  1:1593089/101‑1 (MQ=255)                                                                                           gcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCg                                >  2:339088/1‑139 (MQ=255)                                                                                           gcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTtgtg                                                                                                       >  2:1195831/1‑67 (MQ=255)                                                                                           gcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTtgtg                                                                                                       <  1:1195831/67‑1 (MQ=255)                                                                                           gcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGc                               <  2:460711/139‑1 (MQ=255)                                                                                           gcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGc                               <  2:1069543/139‑1 (MQ=255)                                                                                            cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCg                                         >  1:910746/1‑128 (MQ=255)                                                                                            cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCg                                         <  2:910746/128‑1 (MQ=255)                                                                                                   gTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTtgtg                                                                                                       <  2:1014973/59‑1 (MQ=255)                                                                                                   gTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTtgtg                                                                                                       >  1:1014973/1‑59 (MQ=255)                                                                                                               ttGATTTTGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCttt           >  1:1256690/1‑139 (MQ=255)                                                                                                                      tGAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGACTAGGGATATAATTGATCTCTTCCATAATTTTAGCGATGCGCTCGCCTGTTTCCTTTGGCACCTTTTTCGGCg    <  2:1086593/139‑1 (MQ=255)                                                                                                                       gAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGa   <  1:563144/139‑1 (MQ=255)                                                                                                                       gAAATGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGa   <  1:1203425/139‑1 (MQ=255)                                                                                                                        aaaTGACGGAATCAGGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAg  <  2:1571410/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                              ATCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGA‑GGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAG  >  NC_000913/4490802‑4491059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑