| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,288,554 | G→C | pseudogene (361/2525 nt) | yejO ← | pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,288,554 | 0 | G | C | 97.0% | 226.7 / ‑0.4 | 67 | pseudogene (361/2525 nt) | yejO | pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base C (32/33); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 | |||||||||||
GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG > NC_000913/2288420‑2288688 | gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑Ccccc > 1:220115/1‑139 (MQ=255) aCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑cccccc < 1:969110/139‑1 (MQ=255) aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑‑GCCCCC‑CCATCTTTTTCAAc > 2:501486/1‑139 (MQ=255) cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAAG‑CCCCCC‑CCATCTTTTTCCCCGAtt > 2:97520/1‑139 (MQ=255) gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc < 1:550302/139‑1 (MQ=255) cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCt > 2:1142134/1‑139 (MQ=255) gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCtt > 2:840002/1‑139 (MQ=255) tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATc < 1:97520/139‑1 (MQ=255) cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAtt > 1:208463/1‑139 (MQ=255) cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAtt > 2:236691/1‑139 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc > 2:259591/1‑139 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc > 2:803447/1‑139 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc > 2:198442/1‑139 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc > 2:1003719/1‑139 (MQ=255) ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc > 1:998157/1‑115 (MQ=255) ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc < 2:998157/115‑1 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCGGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGCATCATTCGCTg < 2:874428/139‑1 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 1:406213/1‑139 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg < 1:1003719/139‑1 (MQ=255) gCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcgc < 1:840002/139‑1 (MQ=255) cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc > 2:827269/1‑108 (MQ=255) cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc < 1:827269/108‑1 (MQ=255) tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa > 1:1045194/1‑139 (MQ=255) ttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt < 2:208463/139‑1 (MQ=255) ttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt < 2:1206686/139‑1 (MQ=255) tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATa < 1:621413/139‑1 (MQ=255) cTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa < 1:803447/139‑1 (MQ=255) cTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTg < 2:220115/139‑1 (MQ=255) tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGCCCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTg > 2:1031063/1‑139 (MQ=255) aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTa > 2:1431434/1‑139 (MQ=255) ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg > 1:642343/1‑108 (MQ=255) ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg < 2:642343/108‑1 (MQ=255) ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg < 2:406213/139‑1 (MQ=255) aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGCCCCCCC‑CCATCTTTTTCAACGATTAACCCCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTTTAGCACACCatta > 1:1474534/1‑139 (MQ=255) tctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAg < 1:229742/139‑1 (MQ=255) ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc > 1:1229142/1‑66 (MQ=255) ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc < 2:1229142/66‑1 (MQ=255) ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc > 2:1389589/1‑66 (MQ=255) ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgcc < 1:1389589/66‑1 (MQ=255) ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt > 1:273612/1‑76 (MQ=255) ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt < 2:273612/76‑1 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGttt < 2:72174/136‑1 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGttt > 1:72174/1‑136 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc > 1:1137752/1‑139 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc > 2:520920/1‑139 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca < 2:320488/111‑1 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca > 1:320488/1‑111 (MQ=255) tcctccCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa < 1:146629/139‑1 (MQ=255) ctccCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc > 1:1461790/1‑139 (MQ=255) ctccCTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATAATTCGCTGCGCCATAAAGCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc > 2:970476/1‑139 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgccc > 1:1451771/1‑56 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGcgccc < 2:1451771/56‑1 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 2:1442688/1‑82 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg < 1:1442688/82‑1 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATa < 1:780328/89‑1 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATa > 2:780328/1‑89 (MQ=255) ccTTTTCGATAGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt < 2:1461790/139‑1 (MQ=255) tCGATAGCGACAAAGAC‑‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc < 1:501486/139‑1 (MQ=255) cGATAGCGACAAGGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc > 2:188458/1‑139 (MQ=255) cGATAGCCGCAAAGAAG‑CCCCCCACCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa < 2:1474534/139‑1 (MQ=255) aGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 1:359850/1‑71 (MQ=255) aGCGACAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg < 2:359850/71‑1 (MQ=255) aCAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAt < 1:1431434/139‑1 (MQ=255) cAAAGACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATa < 1:246089/139‑1 (MQ=255) gACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg < 2:672746/139‑1 (MQ=255) gACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg < 2:452610/139‑1 (MQ=255) gACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg < 2:1137752/139‑1 (MQ=255) gACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg < 1:495174/139‑1 (MQ=255) gACG‑CCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg < 1:236691/139‑1 (MQ=255) | GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG > NC_000913/2288420‑2288688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |