Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,401,103	G→A	T151T (ACC→ACT)	nuoF ←	NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,401,103	0	G	A	100.0%	210.9 / NA	60	T151T (ACC→ACT)	nuoF	NADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/31);  total (29/31)

GTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCGGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTG  >  NC_000913/2400968‑2401237                                                                                                                                         |                                                                                                                                       gTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTg                                                                                                                                     <  1:364240/139‑1 (MQ=255)    aGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCt                                                                                                                                  <  1:751083/139‑1 (MQ=255)      cACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTc                                                                                                                                >  2:526262/1‑139 (MQ=255)           cgTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCaa                                                                                                                           >  2:1427209/1‑139 (MQ=255)                ttCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      <  1:526262/139‑1 (MQ=255)                ttCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      <  1:1427209/139‑1 (MQ=255)                      ggAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGgcgc                                                                                                                <  2:977703/139‑1 (MQ=255)                         gTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      >  1:1092063/1‑130 (MQ=255)                         gTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      <  2:1092063/130‑1 (MQ=255)                                  cGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGcc                                                                                                    >  1:1040209/1‑139 (MQ=255)                                       tttCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTc                                                                                               <  1:1193068/139‑1 (MQ=255)                                               ccGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATAtt                                                                                       >  1:1278773/1‑139 (MQ=255)                                                  cAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                                    <  2:616427/139‑1 (MQ=255)                                                  cAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                                    <  1:433053/139‑1 (MQ=255)                                                    gATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCa                                                                                  <  1:1004498/139‑1 (MQ=255)                                                     aTGTAGTGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCAc                                                                                 >  2:1045083/1‑139 (MQ=255)                                                      tGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACg                                                                                <  2:304970/139‑1 (MQ=255)                                                       gTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGc                                                                               >  2:239969/1‑139 (MQ=255)                                                         aGCGCCCTGCCCCGGTATGGAGGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAg                                                                             >  1:731564/1‑139 (MQ=255)                                                          gcgcCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      <  2:184406/97‑1 (MQ=255)                                                          gcgcCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      >  1:184406/1‑97 (MQ=255)                                                               cTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGgcgc                                                                                                                <  1:436887/98‑1 (MQ=255)                                                               cTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGgcgc                                                                                                                >  2:436887/1‑98 (MQ=255)                                                               cTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGAt                                                                       >  2:668804/1‑139 (MQ=255)                                                                tGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATg                                                                      >  1:1400419/1‑139 (MQ=255)                                                                  ccccGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTa                                                                    >  1:89266/1‑139 (MQ=255)                                                                        tATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCAc                                                               >  2:444401/1‑139 (MQ=255)                                                                          tGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGt                                                            <  2:89266/139‑1 (MQ=255)                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                            <  2:648336/121‑1 (MQ=255)                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                            >  1:648336/1‑121 (MQ=255)                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTaa                                                          >  2:535013/1‑139 (MQ=255)                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTaa                                                          >  1:991003/1‑139 (MQ=255)                                                                                              gaaaCCTGTTCCCATAATG‑TTTTTGTCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAAcgcg                                        <  1:1401069/139‑1 (MQ=255)                                                                                                aaCCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGga                                      <  2:991003/139‑1 (MQ=255)                                                                                                aaCCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGga                                      <  2:1161075/139‑1 (MQ=255)                                                                                                 aCCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGgag                                     <  2:1278773/139‑1 (MQ=255)                                                                                                       tCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGc                               >  1:744806/1‑139 (MQ=255)                                                                                                       tCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGc                               <  1:1001762/139‑1 (MQ=255)                                                                                                        cccATAATG‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCa                              <  1:239969/139‑1 (MQ=255)                                                                                                                g‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                                    >  1:63750/1‑77 (MQ=255)                                                                                                                g‑TTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                                    <  2:63750/77‑1 (MQ=255)                                                                                                                    tttGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      >  1:620940/1‑40 (MQ=255)                                                                                                                    tttGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      <  2:620940/40‑1 (MQ=255)                                                                                                                     ttGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCg                                                                                              <  2:1284165/63‑1 (MQ=255)                                                                                                                     ttGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCg                                                                                              >  1:1284165/1‑63 (MQ=255)                                                                                                                      tGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACcag                 >  1:659817/1‑139 (MQ=255)                                                                                                                            gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGcc                                                                                   <  1:407776/67‑1 (MQ=255)                                                                                                                            gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGcc                                                                                   >  2:407776/1‑67 (MQ=255)                                                                                                                            gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGc                                                                  <  2:184343/84‑1 (MQ=255)                                                                                                                            gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGc                                                                  >  1:184343/1‑84 (MQ=255)                                                                                                                            gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAAcg                                          <  1:1158282/108‑1 (MQ=255)                                                                                                                            gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAAcg                                          >  2:1158282/1‑108 (MQ=255)                                                                                                                                aCCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCg                                                                                                   <  1:1396156/47‑1 (MQ=255)                                                                                                                                aCCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCg                                                                                                   >  2:1396156/1‑47 (MQ=255)                                                                                                                                   cGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGt    <  1:668804/139‑1 (MQ=255)                                                                                                                                     cTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCg                                                                                                   <  1:821071/42‑1 (MQ=255)                                                                                                                                     cTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCg                                                                                                   >  2:821071/1‑42 (MQ=255)                                                                                                                                     cTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATAc                           >  2:1038084/1‑114 (MQ=255)                                                                                                                                     cTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATAc                           <  1:1038084/114‑1 (MQ=255)                                                                                                                                     cTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTg  >  2:592920/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       GTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATG‑TTTTTGCCAAGCAGACCCGCTTCGGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTG  >  NC_000913/2400968‑2401237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑