| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,984,452 | (C)5→4 | coding (1141/1182 nt) | yqeF ← | short chain acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,984,448 | 0 | C | . | 96.2% | 101.2 / ‑2.5 | 26 | coding (1145/1182 nt) | yqeF | short chain acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (13/12); minor base T (0/1); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACA > NC_000913/2984319‑2984579 | gggAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGAt > 1:349191/1‑139 (MQ=255) gAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATAc < 1:220768/139‑1 (MQ=255) ccagccaCTATTATTCAACGGATTATTGACGGTAGATATTTATCGGTAAGGAGGAAATGCAGAGCTTTTCGTCACGTTAAATGGTAAATGCCACACCC‑GGTCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCa < 1:74315/139‑1 (MQ=255) cagctaTAATTATTCAACAGATTAAGGAGGGTGAAAATTTTACGGGAAGGAGAAAATGCACAGCTATTCGTCACGTTAAATGTTCAATGCCAAACCCTAG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt < 1:576277/139‑1 (MQ=255) cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa > 1:427966/1‑139 (MQ=255) aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGa > 1:120605/1‑139 (MQ=255) tGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCag > 1:85376/1‑139 (MQ=255) atatTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa > 2:508410/1‑122 (MQ=255) atatTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa < 1:508410/122‑1 (MQ=255) aTCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGccccg > 2:211613/1‑64 (MQ=255) aTCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCg < 2:255087/139‑1 (MQ=255) aTCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTAAATGGTCAATGCCACACCCTGGccccg < 1:211613/65‑2 (MQ=255) aggaggAAATGCAGAGCTATTCGTCACTTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCaga < 1:106623/118‑1 (MQ=255) aggaggAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCaga > 2:106623/1‑118 (MQ=255) ggaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg < 2:85376/139‑1 (MQ=255) ggaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg < 2:119599/139‑1 (MQ=255) aaTGCAGAGCTATTCGTCACGTTCAATGGTCAAAGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcgc > 1:553777/1‑139 (MQ=255) aTGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGcc > 2:258406/1‑139 (MQ=255) aTTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGa < 1:258406/139‑1 (MQ=255) ttCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGa < 1:72532/138‑1 (MQ=255) ttCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGa > 2:72532/1‑138 (MQ=255) ttCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAc > 1:59412/1‑139 (MQ=255) ttCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAc < 2:59412/139‑1 (MQ=255) cAATGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATc > 1:422147/1‑139 (MQ=255) tgttaaaTGCCCCACCCT‑GACACCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATACGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcgcg < 1:226258/134‑1 (MQ=255) tGGTCAATGCCACACCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcgcg < 2:376397/139‑1 (MQ=255) cacCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgaca > 1:440936/1‑139 (MQ=255) acCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg > 1:595472/1‑97 (MQ=255) acCCTGG‑CCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg < 2:595472/97‑1 (MQ=255) | GGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACA > NC_000913/2984319‑2984579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |