| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,104,949 | C→T | intergenic (+47/‑12) | csgA → / → csgC | curlin subunit, amyloid curli fibers, cryptic/curli assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,104,949 | 0 | C | T | 97.8% | 156.4 / ‑3.3 | 46 | intergenic (+47/‑12) | csgA/csgC | curlin subunit, amyloid curli fibers, cryptic/curli assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (20/25); minor base . (0/1); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGC > NC_000913/1104816‑1105077 | gctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgag < 1:227553/139‑1 (MQ=255) ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgaga < 1:460480/139‑1 (MQ=255) gACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATg > 2:507250/1‑139 (MQ=255) cAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgag > 1:226580/1‑127 (MQ=255) cAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgag < 2:226580/127‑1 (MQ=255) ctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGc > 2:206095/1‑139 (MQ=255) tccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCg < 2:358119/139‑1 (MQ=255) ccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg > 2:457661/1‑139 (MQ=255) ccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg < 1:461257/139‑1 (MQ=255) aCTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGt > 2:531608/1‑139 (MQ=255) gTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTT‑GGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATAc < 1:216964/139‑1 (MQ=255) caacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGAt > 1:89381/1‑124 (MQ=255) caacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGAt < 2:89381/124‑1 (MQ=255) aacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGAcc < 1:531608/139‑1 (MQ=255) gCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg < 1:206095/139‑1 (MQ=255) agaaGTACTTATTCATCATTTGTATTACAGAAACTGGGCGCAAGCCCTGTTTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCTTTTTCCAGTCAGATAACCTTTAATACGACCCTGCAAGGGGAt < 1:429581/136‑1 (MQ=255) tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTgg < 2:484416/52‑1 (MQ=255) tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc > 1:201475/1‑139 (MQ=255) aTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCat > 1:175600/1‑139 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgag < 1:524954/48‑1 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgag > 2:524954/1‑48 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa < 1:474073/122‑1 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa > 2:474073/1‑122 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc > 2:69803/1‑139 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc > 1:423089/1‑139 (MQ=255) atTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgag < 1:264870/46‑1 (MQ=255) atTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGgag > 2:264870/1‑46 (MQ=255) tGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc > 1:168151/1‑75 (MQ=255) tGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc < 2:168151/75‑1 (MQ=255) tATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGt < 2:175600/139‑1 (MQ=255) ttACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg > 1:249093/1‑112 (MQ=255) ttACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg < 2:249093/112‑1 (MQ=255) gAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg > 2:284885/1‑107 (MQ=255) gAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg < 1:284885/107‑1 (MQ=255) gAAACAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAAGGGATGTGTATACCATTATTCCTGAAGTCACTCtt < 1:507250/139‑1 (MQ=255) aaCAGGGCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTAc < 2:201475/139‑1 (MQ=255) gggCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATa < 2:239938/76‑1 (MQ=255) gggCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATa > 1:239938/1‑76 (MQ=255) gggCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg > 1:81413/1‑98 (MQ=255) gggCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg < 2:81413/98‑1 (MQ=255) ggCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACt < 2:205578/50‑1 (MQ=255) ggCGCAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACt > 1:205578/1‑50 (MQ=255) cAAGCCCTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGGGGCACTTTCCAGTTAGATAACCTTTAATACGAACCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTg > 2:273590/1‑139 (MQ=255) cTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg < 2:423089/139‑1 (MQ=255) cTG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg < 1:457661/139‑1 (MQ=255) tG‑TTTTTTTTTGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc < 1:69803/139‑1 (MQ=255) | GCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGC > NC_000913/1104816‑1105077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |