| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,580,930 | (A)9→8 | noncoding (87/90 nt) | ryhB ← | sRNA antisense regulator mediating positive Fur regulon response, Hfq‑dependent, global iron regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,580,922 | 0 | A | . | 94.8% | 213.7 / 7.5 | 59 | intergenic (‑117/+5) | yhhX/ryhB | putative oxidoreductase/sRNA antisense regulator mediating positive Fur regulon response, Hfq‑dependent, global iron regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base . (30/25); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCAAAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCAC > NC_000913/3580792‑3581045 | cAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCaaaaaaaag > 1:62049/1‑138 (MQ=255) aGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCaaaaaaaagc < 2:769272/139‑3 (MQ=255) atgaCCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCa > 1:957851/1‑139 (MQ=255) cATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCgg < 1:858885/139‑1 (MQ=255) tGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCggct > 1:748326/1‑139 (MQ=255) tGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCgg > 2:606805/1‑137 (MQ=255) tGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCgg < 1:606805/137‑1 (MQ=255) aTTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCggctgg < 2:726276/139‑1 (MQ=255) aTTTCGTCCTTATTAAGGTGTTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATATATTGAGATCGAAAGAT‑AAAAAAAAAGCCAGCTCCCggctgg < 2:475420/139‑1 (MQ=255) ccTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTa < 2:748326/139‑1 (MQ=255) ttttAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCaaaaaaaagc < 2:551977/115‑3 (MQ=255) ttttAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCaaaaaaaagc > 1:551977/1‑113 (MQ=255) taaataaccacCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCaa > 2:1152104/9‑139 (MQ=255) gtTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCaa > 2:1024159/1‑139 (MQ=255) ccTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAt > 2:698469/1‑130 (MQ=255) ccTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAt < 1:698469/130‑1 (MQ=255) ccTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAAt > 1:344137/1‑139 (MQ=255) ccTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAAt > 2:823521/1‑139 (MQ=255) tAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTc > 1:1078651/1‑139 (MQ=255) tAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTc > 2:818565/1‑139 (MQ=255) aGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCggctggc < 1:677705/106‑1 (MQ=255) aGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCggctggc > 2:677705/1‑106 (MQ=255) cgcAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTg < 1:895792/139‑1 (MQ=255) aGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCAAAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCttt > 2:337419/1‑139 (MQ=255) aGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCAAAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCttt < 1:337419/139‑1 (MQ=255) aGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCg > 1:22263/1‑93 (MQ=255) aGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCg < 2:22263/93‑1 (MQ=255) aGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCg > 1:457902/1‑132 (MQ=255) aGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCg < 2:457902/132‑1 (MQ=255) aCTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAggg > 2:167883/1‑139 (MQ=255) gAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAg > 1:533539/1‑83 (MQ=255) gAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAg < 2:533539/83‑1 (MQ=255) tGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCt > 1:748972/1‑139 (MQ=255) cgcATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGA‑CCAAAAAAATGCCAGCACCCGGCTGGCCAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGa > 2:1171939/1‑139 (MQ=255) aTAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATcg < 2:62049/139‑1 (MQ=255) cGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTg < 1:709504/102‑1 (MQ=255) cGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTg > 2:709504/1‑102 (MQ=255) gAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCgaga < 2:748972/139‑1 (MQ=255) gCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAgg > 2:317213/1‑113 (MQ=255) gCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAgg < 1:317213/113‑1 (MQ=255) cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCggc > 2:403702/1‑51 (MQ=255) cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCggc < 1:403702/51‑1 (MQ=255) cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGa > 2:244321/1‑108 (MQ=255) cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGa < 1:244321/108‑1 (MQ=255) cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAgg < 2:278714/110‑1 (MQ=255) cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAgg > 1:278714/1‑110 (MQ=255) cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAAtc > 1:842351/1‑139 (MQ=255) tCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAt < 2:521056/74‑1 (MQ=255) tCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAt > 1:521056/1‑74 (MQ=255) tCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCga < 2:97338/125‑1 (MQ=255) tCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCga > 1:97338/1‑125 (MQ=255) ccGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAAtcatt > 2:424360/1‑139 (MQ=255) ccGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAAtcatt < 1:1148950/139‑1 (MQ=255) aTAAATTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAAtcattctcatt < 1:424360/139‑1 (MQ=255) aaaTTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCg > 2:495600/1‑139 (MQ=255) aTTGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCa > 2:447096/1‑139 (MQ=255) ttGAGAACGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCAc < 1:1191537/139‑1 (MQ=255) aaCGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAgg < 2:753970/91‑1 (MQ=255) aaCGAAAGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGCAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAgg > 1:753970/1‑91 (MQ=255) aaGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCttt < 2:582824/70‑1 (MQ=255) aaGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCttt > 1:582824/1‑70 (MQ=255) aaGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCgaga > 2:279114/1‑104 (MQ=255) aaGATC‑AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCgaga < 1:279114/104‑1 (MQ=255) | CAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATCAAAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCAC > NC_000913/3580792‑3581045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |