| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,763,869 | (T)7→8 | coding (1285/1617 nt) | yfjL ← | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,763,862 | 1 | . | T | 96.6% | 195.6 / ‑0.8 | 59 | coding (1292/1617 nt) | yfjL | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (29/28); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
ATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTT > NC_000913/2763735‑2763991 | aTTAGAGTCGTAGCCCACGAACAGAACAAACGTTAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttttt‑t‑ttgcg < 2:113746/139‑5 (MQ=255)aTTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttttt‑t‑ttgcg < 1:353207/139‑5 (MQ=255) tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑Tgcgcg > 1:984868/1‑139 (MQ=255) tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑Tgcgcg < 2:771482/139‑1 (MQ=255) tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTa > 2:873867/1‑139 (MQ=255) gTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATc > 2:139598/1‑139 (MQ=255) aaaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTc < 1:873867/139‑1 (MQ=255) aaaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTTTGT‑TGCGCGCGGTATCGATGCAATCCGAAATGTc < 1:536582/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:984868/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:261117/139‑1 (MQ=255) aTCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTg > 2:1112539/1‑139 (MQ=255) cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGAt < 1:139598/139‑1 (MQ=255) cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTTT‑TGTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGAt > 2:404035/1‑139 (MQ=255) gCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGt > 2:855865/1‑122 (MQ=255) gCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGt < 1:855865/122‑1 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAtct > 1:415742/1‑139 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAtct > 1:773189/1‑139 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATCCAATCCGAAATGTCCTCGTACAACTGATCCCGTAtct > 2:789664/1‑139 (MQ=255) cGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCg > 1:1101389/1‑139 (MQ=255) gAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCgg > 2:565210/1‑139 (MQ=255) aaaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑Tgcgcg < 2:463909/86‑1 (MQ=255) aaaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑Tgcgcg > 1:463909/1‑86 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 1:999133/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:1127600/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 1:1189229/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:1226558/139‑1 (MQ=255) aTCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTc < 2:235588/97‑1 (MQ=255) aTCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTc > 1:235588/1‑97 (MQ=255) aTCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCATATCTCCGGCAATCTGACGGCAAt > 2:593014/1‑139 (MQ=255) tCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGa < 2:569146/122‑1 (MQ=255) tCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGa > 1:569146/1‑122 (MQ=255) tCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTc > 1:69849/1‑139 (MQ=255) aTGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑Tgcgcg < 2:1002633/56‑1 (MQ=255) aTGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑Tgcgcg > 1:1002633/1‑56 (MQ=255) tGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCa > 1:182518/1‑139 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCg < 2:1199123/68‑1 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCg > 1:1199123/1‑68 (MQ=255) aaGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGa < 2:674561/54‑1 (MQ=255) aaGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGa > 1:674561/1‑54 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc < 2:1101389/139‑1 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc < 2:773189/139‑1 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc < 1:404035/139‑1 (MQ=255) gTTATCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCa < 2:415742/139‑1 (MQ=255) tCGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCATATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGc < 1:593014/139‑1 (MQ=255) cGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAAt < 2:316215/46‑1 (MQ=255) cGTCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAAt > 1:316215/1‑46 (MQ=255) tCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCaa < 2:373239/133‑1 (MQ=255) tCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCaa > 1:373239/1‑133 (MQ=255) tCTTTAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa > 2:57956/1‑139 (MQ=255) tttAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:170831/92‑1 (MQ=255) tttAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa > 1:170831/1‑92 (MQ=255) tttAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATc < 1:57956/139‑1 (MQ=255) tttAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATc > 2:1140875/1‑139 (MQ=255) ttAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACgg > 1:120318/1‑89 (MQ=255) ttAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACgg < 2:120318/89‑1 (MQ=255) tAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTcc > 1:540659/1‑51 (MQ=255) tAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:540659/51‑1 (MQ=39) tAATGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCAc > 1:117253/1‑139 (MQ=255) tGTCGAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCtt > 2:1232166/1‑139 (MQ=255) gAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa > 1:1041152/1‑83 (MQ=255) gAGGATTTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:1041152/83‑1 (MQ=255) | ATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTTT‑T‑TGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTT > NC_000913/2763735‑2763991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |