Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,401,103	G→A	T151T (ACC→ACT)	nuoF ←	NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,401,103	0	G	A	100.0%	184.2 / NA	53	T151T (ACC→ACT)	nuoF	NADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/27);  total (26/27)

CACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCGGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGC  >  NC_000913/2400973‑2401232                                                                                                                                   |                                                                                                                                   cACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTc                                                                                                                           >  2:1337310/1‑139 (MQ=255)      cgTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCaa                                                                                                                      >  1:979088/1‑139 (MQ=255)                 ggAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGgcgc                                                                                                           <  1:873478/139‑1 (MQ=255)                   aGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAg                                                                                                         >  1:895140/1‑139 (MQ=255)                              gCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTGCCATAATGTTTTTGCCAAGCAGAACCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCATATTAACGGCCg                                                                                              <  1:958100/139‑1 (MQ=255)                                tGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCt                                                                                            <  1:1337310/139‑1 (MQ=255)                                  tttCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTc                                                                                          >  1:518148/1‑139 (MQ=255)                                  tttCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTc                                                                                          <  1:1592056/139‑1 (MQ=255)                                            gCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCg                                                                                <  2:166792/139‑1 (MQ=255)                                             cAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                               <  2:1638690/139‑1 (MQ=255)                                             cAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                               <  1:1117869/139‑1 (MQ=255)                                                     gcgcCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       <  2:363844/139‑1 (MQ=255)                                                      cgcCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGa                                                                      <  1:615671/139‑1 (MQ=255)                                                                       gACGCACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAA‑CAAGCCCGCTTCAGTGGCCTTCGG‑AATGGCACGGCGCAGGTTCACTGCCGCTTCGATCTAATCGACACCCAAGGAGTTTGAGCCACGGttaa                                                    >  1:1400438/1‑136 (MQ=255)                                                                       gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTaa                                                     >  1:1191761/1‑139 (MQ=255)                                                                       gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTaa                                                     >  1:765381/1‑139 (MQ=255)                                                                            aCAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCttt                                                >  1:1447481/1‑139 (MQ=255)                                                                            aCAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCttt                                                >  1:387885/1‑139 (MQ=255)                                                                             cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       >  1:1203481/1‑115 (MQ=255)                                                                             cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       <  2:1203481/115‑1 (MQ=255)                                                                             cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTc                                               >  2:151375/1‑139 (MQ=255)                                                                               gTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       >  2:931231/1‑113 (MQ=255)                                                                               gTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       <  1:931231/113‑1 (MQ=255)                                                                               gTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       <  1:1208349/113‑1 (MQ=255)                                                                               gTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       >  2:1208349/1‑113 (MQ=255)                                                                                  cgaaatcgcaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAgcgc                                          <  1:1002497/139‑1 (MQ=255)                                                                                         gaaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAAcgcg                                   <  1:1248227/139‑1 (MQ=255)                                                                                          aaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACg                                                                           >  2:315715/1‑98 (MQ=255)                                                                                          aaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACg                                                                           <  1:315715/98‑1 (MQ=255)                                                                                          aaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCgg                                  >  1:828962/1‑139 (MQ=255)                                                                                           aaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGga                                 <  1:70145/139‑1 (MQ=255)                                                                                               tGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCt                                                                                            <  1:487240/76‑1 (MQ=255)                                                                                               tGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCt                                                                                            >  2:487240/1‑76 (MQ=255)                                                                                                 ttCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       >  2:219484/1‑95 (MQ=255)                                                                                                 ttCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       <  1:219484/95‑1 (MQ=255)                                                                                                 ttCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAg                           >  2:1455098/1‑139 (MQ=255)                                                                                                   cccATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCa                         <  2:883054/139‑1 (MQ=255)                                                                                                   cccATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCa                         <  2:518148/139‑1 (MQ=255)                                                                                                   cccATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCa                         <  2:377522/139‑1 (MQ=255)                                                                                                   cccATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCa                         <  2:979088/139‑1 (MQ=255)                                                                                                      aTAATGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATAc                      >  2:642655/1‑139 (MQ=255)                                                                                                         aTGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCg                                                                                <  2:1142859/78‑1 (MQ=255)                                                                                                         aTGTTTTTGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCg                                                                                >  1:1142859/1‑78 (MQ=255)                                                                                                              tttGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCAc                                                          >  1:57076/1‑95 (MQ=255)                                                                                                              tttGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCAc                                                          <  2:57076/95‑1 (MQ=255)                                                                                                              tttGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACG‑‑GCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCAc                                                          >  1:515464/1‑93 (MQ=255)                                                                                                              tttGCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACG‑‑GCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCAc                                                          <  2:515464/93‑1 (MQ=255)                                                                                                                 gCCAAGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACcagc           >  1:1073346/1‑139 (MQ=255)                                                                                                                    aaGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                               <  2:1372009/68‑1 (MQ=255)                                                                                                                    aaGCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                               >  1:1372009/1‑68 (MQ=255)                                                                                                                      gCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGa                                                                                        <  2:1128971/57‑1 (MQ=255)                                                                                                                      gCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGa                                                                                        >  1:1128971/1‑57 (MQ=255)                                                                                                                      gCAGACCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTg      >  1:231909/1‑139 (MQ=255)                                                                                                                          aCCCGCTTCAGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGc  >  2:759116/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                   CACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCGGTGG‑CTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGC  >  NC_000913/2400973‑2401232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑