| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.3% | 132.7 / ‑3.0 | 37 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (19/17); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01 | |||||||||||
TGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTG > NC_000913/1399840‑1400096 | tGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGt < 2:638194/139‑1 (MQ=255) aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccc < 2:617729/139‑1 (MQ=255) tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccccc > 2:179057/1‑139 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccccc‑ccg > 1:946170/1‑137 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAg < 2:1114034/139‑1 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAg > 2:957851/1‑139 (MQ=255) aaCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGt < 1:179057/139‑1 (MQ=255) aaCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGt < 1:82820/139‑1 (MQ=255) cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCaa < 2:1048389/139‑1 (MQ=255) aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCaat > 1:693240/1‑139 (MQ=255) cgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCaataat > 2:118426/1‑139 (MQ=255) gACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTAc > 1:327043/1‑139 (MQ=255) gCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCaa < 2:1095336/120‑1 (MQ=255) gCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCaa > 1:1095336/1‑120 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGa < 2:994842/109‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGa > 1:994842/1‑109 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 1:548614/1‑139 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 1:507928/1‑139 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa < 1:787986/139‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGT‑GGAAGCGTATCAATAATGCTAAGATTACCGGCaa > 1:142991/1‑139 (MQ=255) tCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCa < 2:656536/139‑1 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 2:693240/139‑1 (MQ=255) aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 1:668691/139‑1 (MQ=255) aTTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGt > 2:1120018/1‑128 (MQ=255) aTTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGt < 1:1120018/128‑1 (MQ=255) aTTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg > 1:622281/1‑139 (MQ=255) tGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGa < 1:434923/139‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt < 2:548614/139‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 1:589171/1‑139 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 1:584478/1‑139 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 1:258581/1‑139 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc < 2:507928/139‑1 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcg > 2:47748/1‑139 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGT‑GGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 2:142991/139‑1 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCt < 2:380301/139‑1 (MQ=255) ttttCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGc > 1:962386/1‑139 (MQ=255) tttCCAGACTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGcc > 1:55553/1‑139 (MQ=255) aCTTCGCGCTGAAATGGCGTC‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTg > 1:614099/1‑139 (MQ=255) aCTTCGCGCTGAAATGGCGT‑‑CCCCCCTCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTg > 2:1021050/1‑139 (MQ=255) cgcgCTGAAATGGCGTCCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 2:437103/118‑1 (MQ=255) cgcgCTGAAATGGCGTCCCCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg > 1:437103/1‑118 (MQ=255) | TGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCCC‑CGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTG > NC_000913/1399840‑1400096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |