Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,419,861	G→A	18.6%	pseudogene (25/81 nt)	EK12AG100_RS25785 →	paraslipin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,419,861	0	G	A	18.6%	165.9 / 32.4	83	pseudogene (25/81 nt)	EK12AG100_RS25785	paraslipin
Reads supporting (aligned to +/- strand):  ref base G (28/39);  new base A (3/11);  total (33/50)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

GGGGTTTGCACGAACCGTCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACT  >  NZ_LN832404/3419568‑3420154                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       ggggTTTGCACGAACCGTCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTAgg                                                                                                                                                                                                                                                                                                       >  1:428724/1‑295 (MQ=255)   ggTTTGCACGAACCGTCGCTGACCGGGTAATTGTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCTTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGga                                                                                                                                                                                                                                                                                                     <  1:571564/295‑1 (MQ=255)     gttGCACGAACCGTGGCTTAGCGGTGAATTTTTATGGAGCGTGGGGAAATAGTGGAGCAAGCGGCACCTGATGAATTGTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGgaga                                                                                                                                                                                                                                                                                                   <  2:172592/294‑1 (MQ=255)        gtaggAACCGTCGGTGAACGGGGAATTTTTATGGAGCGGGGGGAAATAGTGGAGCAAGCTGCATCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGTA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCGGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAgtg                                                                                                                                                                                                                                                                                                <  2:601782/291‑1 (MQ=255)         actacgcgtactgtgagccagagttgcccggcgctctccggctgcggtagttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAGTTGTCCTACTCCGgagagc                                                                                                                                                                                                                                                                                                 >  1:112630‑M2/198‑294 (MQ=255)          aCGAACTGTCGTTTACCGGGTAATTTTTGTGTGTCGTGGGGAAATAGTAGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGGGCGTACGAGGGCA‑TTTTGATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGtt                                                                                                                                                                                                                                                                                              <  2:485809/295‑1 (MQ=255)            acgcgtactgtgagccagagttgcccggcgctctccggctgcggtagttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGtt                                                                                                                                                                                                                                                                                              <  1:574743‑M2/101‑1 (MQ=255)             aaCCGTCGCTTACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGTATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCAc                                                                                                                                                                                                                                                                                           <  1:737519/295‑1 (MQ=255)             aaCCGTCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCAc                                                                                                                                                                                                                                                                                           <  1:119595/295‑1 (MQ=255)                ggTCGCTGACCGGGTAATTTTGATGGATCGTGGGGAAATAGGGGGGCAAGCGGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGa                                                                                                                                                                                                                                                                                        <  2:159562/294‑1 (MQ=255)                  tCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCACATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCCCCGacc                                                                                                                                                                                                                                                                                      >  1:429321/1‑294 (MQ=255)                   cGCTGACCTGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGacaa                                                                                                                                                                                                                                                                                     <  1:230413/295‑1 (MQ=255)                         ccGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTTGGGAGAGTTTTCACCGACAAacaaca                                                                                                                                                                                                                                                                               >  1:620133/1‑295 (MQ=255)                            ggTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTTCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACCGAt                                                                                                                                                                                                                                                                            >  1:8479/1‑295 (MQ=255)                                  gcccggcgctctcctgctgcggtagttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcactgcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa                                                                                                                                                                                                                                                                        <  1:334588‑M2/123‑1 (MQ=255)                                   cccggcgctctccggctgcggtagttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAAc                                                                                                                                                                                                                                                                       <  1:68146‑M2/124‑1 (MQ=255)                                     cggcgtctcccggcttcggtacgtcaggcattgcaatcaactgtttaccttggggggcgacagccagaggcacttcaccgcttgccagccgcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAaacga                                                                                                                                                                                                                                                                     <  2:469840‑M2/126‑3 (MQ=255)                                        tgctctccggctggggtagttcaggcaggtcaatcaactgtttaccttgtggagcgacatccagagtcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg                                                                                                                                                                                                                                                                  <  1:696922‑M2/129‑1 (MQ=255)                                          aTCGTTGGGAAATAGTGGTGATAGCTGCACCTGATGAATGTTTTGCGCATCTGAAATCAGAGGTTACGAGGTTG‑TGTTTATCGAAGGTAATCCCTTAAGTGAATGTTATTTCGAAAAGCAAAAAGGTCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGccc                                                                                                                                                                                                                                                              <  2:667459/295‑1 (MQ=21)                                             tgggggAAATAGTGGAGCAAGCTGTAACTGATGAATTTTTTGCGAATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATGGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGt                                                                                                                                                                                                                                                           <  1:136108/293‑1 (MQ=255)                                              tGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCCGGCCGTTGCTTCGCAACGTTCCACTCCGCTCCCGGCGGATTTGTCCGACTCGGGAGAGTGTTCACCGACCAACAACCAATAAAACACACAGCCCAgcc                                                                                                                                                                                                                                                          >  2:326377/1‑293 (MQ=255)                                                gggAAATATTGGAGCAAGCTGCACCTGATGAATTTTTTGCTCATCCTATATCAGAGCGTACGAGGGCA‑TTTTTATCGCATGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGTCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGTCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCtt                                                                                                                                                                                                                                                        <  1:82863/295‑1 (MQ=255)                                                gggAAATAGTGGAGCAAGCTGCACCGGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCtt                                                                                                                                                                                                                                                        <  1:182401/295‑1 (MQ=255)                                                  gAAATCGTGGAGCAAGTGGCACCTGATGAATTTTTTTGGCATCCTTAATCAGGGCTTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATTTTATTGCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTcc                                                                                                                                                                                                                                                      <  2:273227/295‑1 (MQ=21)                                                  gAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCACCGTTCAAATCCCCCCCCGGCGCGTTTGTCCTTCCCCGGAGCGTGTTCCCCCTCCAACCACCGACAAAACCAAAGTCCCCGtcctcc                                                                                                                                                                                                                                                      >  2:91161/1‑295 (MQ=255)                                                    gcgggagttcaggcagttcaatcaactgtttacctggtggagcgacatccaggggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGtctttc                                                                                                                                                                                                                                                      <  1:257718‑M2/141‑3 (MQ=255)                                                      tattGGATCAAGGTGATCCTGATGAATGTTTGGCGGAGCCTACATCTGAGCGTACGAGGGCATTTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTGGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                                                                                                                                                                                                                                                   <  2:425807/292‑1 (MQ=21)                                                      tAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACt                                                                                                                                                                                                                                                  <  1:683150/295‑1 (MQ=255)                                                          gttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa                                                                                                                                                                                                                                                <  1:753732‑M2/147‑1 (MQ=255)                                                                gcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTAACCGACCAACAAACGGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCatt                                                                                                                                                                                                                                          >  1:333934‑M2/143‑295 (MQ=255)                                                                   gttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGt                                                                                                                                                                                                                                       >  1:315417‑M2/140‑295 (MQ=255)                                                                      caatctaccgctttccttgttgctcgtcagctagcggcacgtcaccgcttgtcagcggcttacaatccggcgccaccatccagtgcaggagctccgatgctaagctggcaaacatgagaattccgatccccaattcCTGGCAGTGTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGTGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTTTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtttt                                                                                                                                                                                                                                    <  2:749760‑M2/159‑1 (MQ=255)                                                                        atcaactgtttcccttgtggagcgacatccagaggcccttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAt                                                                                                                                                                                                                                  <  1:293050‑M2/161‑1 (MQ=255)                                                                         ttatgaATTTTTTGCGCATCCTAAATCATGGCGTACGAGGGCA‑TTTTTATCGCAGTTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTg                                                                                                                                                                                                                               <  1:125322/293‑1 (MQ=255)                                                                          gatgaATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGa                                                                                                                                                                                                                              >  1:294844/1‑295 (MQ=255)                                                                               gtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGAtgc                                                                                                                                                                                                                           <  1:129515‑M2/168‑2 (MQ=255)                                                                               aTTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt                                                                                                                                                                                                                         >  1:410132/1‑295 (MQ=255)                                                                                    ccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCtggg                                                                                                                                                                                                                      >  1:135069‑M2/123‑294 (MQ=255)                                                                                     cttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctggcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCctgggg                                                                                                                                                                                                                     <  2:86226‑M2/174‑3 (MQ=255)                                                                                      gcgcATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAACGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGtt                                                                                                                                                                                                                  >  1:587636/1‑295 (MQ=255)                                                                                           tCCTAAATAAGGGCGTCCGAGGCCA‑TTGTTAGCCAAGGTAATCCATTATGTAAATTGTAGTTCGAAAAGAAAAAAGGCCT‑TCTTGCCGGTTGGCGTTTCGCTTGATTTGATGTCTGGCAGTTCATGGCGGGC‑GTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTa                                                                                                                                                                                                             <  2:271673/295‑1 (MQ=17)                                                                                               gacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCCGCGGATTTGTCCTACTCCTGAGAGCGTTCACCGACAAACAACAGCTAAAACGAAACGCCCAAGCTCTCGTATAACCCTTTCGTTTtattttatgcctgggggatttatttc                                                                                                                                                                                                           >  2:162709‑M2/112‑283 (MQ=255)                                                                                                    agagCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCCCCCGGCGGATTTGTCCTACCCGGGAGAGTGTTCACCGACAAACCACAGGTAAAACAAAAAGCCCAGTCTTCCGGACGAACCTTTTGGTTTATTTGATTGCTGGCCTTTACCTtctccccgct                                                                                                                                                                                                    >  2:276315/1‑289 (MQ=18)                                                                                                          tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCCACGTTCCAATCCCCTCCCGGCGGATTTGGCCTACTCGGGAGAGTGTTCACCGACCACCAACCCATAAAACAAAAGTCCCAACCTTCCGACTGCGCCTTTTGCTTTATTTTATGTCTAGCAGTTCCCTTCTCTCCCCTGGGgac                                                                                                                                                                                              >  2:243085/1‑294 (MQ=255)                                                                                                          tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCCTCTCGCATGGGgag                                                                                                                                                                                              >  1:404774/1‑295 (MQ=255)                                                                                                          tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATggggcg                                                                                                                                                                                              >  1:546715/1‑293 (MQ=255)                                                                                                                       tttAACGCAGTGAATCCATAAATTGAATGTTCTGTCGAAAAGCAAAATGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGAGTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTAc                                                                                                                                                                                 <  2:241987/295‑1 (MQ=255)                                                                                                                           tCGCAGGTAATCCATTAATTGAATGTTATTTCGAAAGGCAAAACGGCCT‑TCCTTTCGGATGGCCTTTCGCTTGATTTTATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATc                                                                                                                                                                             <  1:63530/295‑1 (MQ=255)                                                                                                                                    aTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACC‑ATACCATCGGCGCTACg                                                                                                                                                                    >  1:701122/1‑295 (MQ=255)                                                                                                                                      caaTTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCTTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGc                                                                                                                                                                  <  1:685384/293‑1 (MQ=255)                                                                                                                                          tAATTGAAAGTTAGTTCAAAAAGAAAAAAGGGCT‑GGCTTTCGGATGGTCTTTGGCTTGATTTGCTTGTTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGttt                                                                                                                                                              <  2:427046/295‑1 (MQ=14)                                                                                                                                             ttGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTAACCACGGCGCTACGGCGGTTCAc                                                                                                                                                           >  1:186248/1‑295 (MQ=255)                                                                                                                                                     cggTTAGAAAAGAAAAAAGGCAC‑TCCTTTCGGTGGGCCTTTGGCTTGATTTGATGTCTGGCAGTGTACGGCGGGC‑GTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                                                                                                                                                   <  2:8479/293‑1 (MQ=14)                                                                                                                                                      aGTTCGACAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                                                                                                                                                  >  1:447743/1‑295 (MQ=255)                                                                                                                                                      aGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCCCTTCTGAGtt                                                                                                                                                  >  1:692308/1‑295 (MQ=255)                                                                                                                                                        gtCGCAAAGCCAAAAGGGCACTCCTTTGTGGTGGCTTTGTGCGTGATTTGATGTCGGGCGGGTTATGCCGGGC‑GTCCTGCCCGCAACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                                                                                                                                 <  2:547051/294‑1 (MQ=14)                                                                                                                                                                     aaGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTTTGGCAGTTCCCTACTCTCGCCAGGTGACACCCCACC‑ATACCCTCGGCACCACGGCCATTTCCTTCTGTGTTCGGctacgggccagg                                                                                                                                   >  2:214584/1‑284 (MQ=14)                                                                                                                                                                         gcctgccTTTCGGCGGGCCTTTCGCTGGATTGGATGGCGGGGAGTGTATTGCGGGC‑GGCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                                                                                                                <  2:699705/290‑1 (MQ=14)                                                                                                                                                                           a‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCCGGAGGGTTTTCCCCGACAAACCACAGATAAAACCAAAGGCCCAATCTTCCGACCGAGCCTTTTGTTTTTTTTGATGTCTGGCAGTTCCCTACCCTCTCATGGGG‑GTCCCCCCACCTACCATCGGCCCTACCGCCGTTCACTTCTGGCTTTGGCCTCGGGTCAGGTgggtc                                                                                                                             >  2:153646/1‑293 (MQ=14)                                                                                                                                                                           a‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGAGCCGTTGCTTCGCAACGTTCCAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTCTTTGATGTCTGGCAGTTCCCTACTCTCGCCTGGGGAGGCCCCACC‑CTACCACCCGCCCTACCGGCTTTCCCTTCTTGTTTAGCCCTTGGGTCCGGTgggtc                                                                                                                             >  2:88110/1‑293 (MQ=14)                                                                                                                                                                              ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCGGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTCTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGacca                                                                                                                           <  1:579000/295‑1 (MQ=255)                                                                                                                                                                                tttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc                                                                                                                         >  1:279436/1‑295 (MQ=255)                                                                                                                                                                                   cGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCCGGTCCCTACCCTCGCATGGGCATCCCCCACA‑CTAACCTCCGCCCTACGGCGCTTCCCTTCTTCGTTCCGCCCGGGGACCGGTggccgcaccggc                                                                                                                      >  2:304641/1‑285 (MQ=14)                                                                                                                                                                                      aTGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACCCGGGAGAGTGTTCACCGACAAACCACCGATAAAACAAAAGGCCCAGTCTTCCGACCGAACCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCCCATGGGGAGACCCCACC‑CTACCCACCGCGCTACGGCGGTTCCCTTCTTGCTTCGGCCTTGGGCCCAGTGGGACCACCGCGCtc                                                                                                                   >  2:534826/1‑294 (MQ=14)                                                                                                                                                                                                cctggttttgatGTCGGGCCGTTT‑TGGTGCGCGGCCCTGCCCGCCACCCTCCGTGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTAACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAg                                                                                                         <  2:607689/289‑1 (MQ=11)                                                                                                                                                                                                 cTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGa                                                                                                        >  1:331319/1‑295 (MQ=39)                                                                                                                                                                                                  ttgatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACC‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTTGGCATGGGGTCAGGTGGGACCACCGCCGACCTGCCGCCAgcg                                                                                                       >  2:52161/1‑293 (MQ=255)                                                                                                                                                                                                    gatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaa                                                                                                     <  1:52161/295‑1 (MQ=39)                                                                                                                                                                                                             tCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                                                                                            >  1:701685/1‑295 (MQ=39)                                                                                                                                                                                                              cTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                                                                                           <  1:721909/295‑1 (MQ=39)                                                                                                                                                                                                                         ttgcgtgc‑gtCCTTCCCGCCGCCCTCCTGGCCGTTGCTTCGCCACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa                                                                                <  1:292071/293‑1 (MQ=255)                                                                                                                                                                                                                            cggtc‑gTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACGCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt                                                                             <  1:180857/295‑1 (MQ=255)                                                                                                                                                                                                                              ggc‑gTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa                                                                           <  1:534826/295‑1 (MQ=255)                                                                                                                                                                                                                                   tCCTGCCCGCCACCCTCCCGGCCGTTGCTTCTCAACGTTCAAATCCGCTCCCTGCGGATTTGTCCTACGCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGTCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCt                                                                       <  1:436220/295‑1 (MQ=255)                                                                                                                                                                                                                                           gCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg                                                               >  1:326789/1‑295 (MQ=255)                                                                                                                                                                                                                                              ccccTCCGGGCCTTTGCCTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtggtg                                                            <  2:385103/294‑1 (MQ=255)                                                                                                                                                                                                                                                    ccggCCGTGCCCTCGCAACGTTCAAATCCGCTCCCGGCGGATTGTCCCTCATCGGGATAGTGTTCACCGACAAACAACAGATAAACCAAAAGGCCCAGTCGTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATa                                                      <  2:186248/293‑1 (MQ=17)                                                                                                                                                                                                                                                           ttGCTTCGCAACGTTCACATCCGCTCCCGGCGGATTTGTCCTACACGGGCGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGTGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCagag                                               <  2:294844/295‑1 (MQ=255)                                                                                                                                                                                                                                                           ttGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCagag                                               <  1:8127/295‑1 (MQ=255)                                                                                                                                                                                                                                                              cTTCGCAACGTTCAAATCAGCGCCCGTCGGATTTGTGCTACTCGGGAGATTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCg                                            <  2:492054/295‑1 (MQ=255)                                                                                                                                                                                                                                                               ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGa                                           <  1:276315/295‑1 (MQ=255)                                                                                                                                                                                                                                                                 cGCAACGTTAAAATCCGCTCCCGGCGGATGTGTCCTACGTGGGAGAGTGTTCACCGACAAACAACAGAGAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGGTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAAc                                         <  2:701685/295‑1 (MQ=255)                                                                                                                                                                                                                                                                           aaaCCCGCTCCCGGCGGATTGTTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGGTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTc                               <  2:69806/295‑1 (MQ=255)                                                                                                                                                                                                                                                                             aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAATGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAc                             >  1:532632/1‑295 (MQ=255)                                                                                                                                                                                                                                                                             aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAACGTGGTTCTGCTACCCAGAGTCGAGCTGGGGACCtacc                             >  1:317479/1‑292 (MQ=255)                                                                                                                                                                                                                                                                              tCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACCAATTCTTTTCTCATCTGCCGAACTTTAACCTAAAACGTGGTGCTGAGTCCCAGAGTCGGCCTGGGGAccccccc                            >  2:288965/1‑290 (MQ=255)                                                                                                                                                                                                                                                                                  cTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTACAAAGTGGTGCTGATACCCAAAGTCGAACTGGGGAACtacccactt                        >  2:694088/1‑287 (MQ=255)                                                                                                                                                                                                                                                                                      cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAACAGTGGTGCTGATACCCAGAGTAGAACTGGGGCCCTCACCCTTACCaa                    >  2:728836/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                      cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGACGAACTGGGGACCTCACCCTTACaaa                    >  1:550088/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                         cggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                 >  1:66107/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                          ggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTACAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACTCTTACCACgggg                >  2:376360/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                                        cGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACt  <  1:288965/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       GGGGTTTGCACGAACCGTCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACT  >  NZ_LN832404/3419568‑3420154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑