Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,419,868	T→C	16.7%	pseudogene (32/81 nt)	EK12AG100_RS25785 →	paraslipin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,419,868	0	T	C	16.7%	168.0 / 30.0	78	pseudogene (32/81 nt)	EK12AG100_RS25785	paraslipin
Reads supporting (aligned to +/- strand):  ref base T (29/36);  new base C (2/11);  total (31/47)
Fisher's exact test for biased strand distribution p-value = 6.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01

GCACGAACCGTCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCAT  >  NZ_LN832404/3419575‑3420161                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       gtaggAACCGTCGGTGAACGGGGAATTTTTATGGAGCGGGGGGAAATAGTGGAGCAAGCTGCATCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGTA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCGGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAgtg                                                                                                                                                                                                                                                                                                       <  2:601782/291‑1 (MQ=255)   aCGAACTGTCGTTTACCGGGTAATTTTTGTGTGTCGTGGGGAAATAGTAGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGGGCGTACGAGGGCA‑TTTTGATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGtt                                                                                                                                                                                                                                                                                                     <  2:485809/295‑1 (MQ=255)     acgcgtactgtgagccagagttgcccggcgctctccggctgcggtagttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGtt                                                                                                                                                                                                                                                                                                     <  1:574743‑M2/101‑1 (MQ=255)      aaCCGTCGCTTACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGTATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCAc                                                                                                                                                                                                                                                                                                  <  1:737519/295‑1 (MQ=255)      aaCCGTCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCAc                                                                                                                                                                                                                                                                                                  <  1:119595/295‑1 (MQ=255)         ggTCGCTGACCGGGTAATTTTGATGGATCGTGGGGAAATAGGGGGGCAAGCGGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGa                                                                                                                                                                                                                                                                                               <  2:159562/294‑1 (MQ=255)           tCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCACATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCCCCGacc                                                                                                                                                                                                                                                                                             >  1:429321/1‑294 (MQ=255)            cGCTGACCTGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGacaa                                                                                                                                                                                                                                                                                            <  1:230413/295‑1 (MQ=255)                  ccGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTTGGGAGAGTTTTCACCGACAAacaaca                                                                                                                                                                                                                                                                                      >  1:620133/1‑295 (MQ=255)                     ggTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTTCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACCGAt                                                                                                                                                                                                                                                                                   >  1:8479/1‑295 (MQ=255)                           gcccggcgctctcctgctgcggtagttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcactgcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa                                                                                                                                                                                                                                                                               <  1:334588‑M2/123‑1 (MQ=255)                            cccggcgctctccggctgcggtagttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAAc                                                                                                                                                                                                                                                                              <  1:68146‑M2/124‑1 (MQ=255)                              cggcgtctcccggcttcggtacgtcaggcattgcaatcaactgtttaccttggggggcgacagccagaggcacttcaccgcttgccagccgcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAaacga                                                                                                                                                                                                                                                                            <  2:469840‑M2/126‑3 (MQ=255)                                 tgctctccggctggggtagttcaggcaggtcaatcaactgtttaccttgtggagcgacatccagagtcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg                                                                                                                                                                                                                                                                         <  1:696922‑M2/129‑1 (MQ=255)                                   aTCGTTGGGAAATAGTGGTGATAGCTGCACCTGATGAATGTTTTGCGCATCTGAAATCAGAGGTTACGAGGTTG‑TGTTTATCGAAGGTAATCCCTTAAGTGAATGTTATTTCGAAAAGCAAAAAGGTCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGccc                                                                                                                                                                                                                                                                     <  2:667459/295‑1 (MQ=21)                                      tgggggAAATAGTGGAGCAAGCTGTAACTGATGAATTTTTTGCGAATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATGGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGt                                                                                                                                                                                                                                                                  <  1:136108/293‑1 (MQ=255)                                       tGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCCGGCCGTTGCTTCGCAACGTTCCACTCCGCTCCCGGCGGATTTGTCCGACTCGGGAGAGTGTTCACCGACCAACAACCAATAAAACACACAGCCCAgcc                                                                                                                                                                                                                                                                 >  2:326377/1‑293 (MQ=255)                                         gggAAATATTGGAGCAAGCTGCACCTGATGAATTTTTTGCTCATCCTATATCAGAGCGTACGAGGGCA‑TTTTTATCGCATGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGTCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGTCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCtt                                                                                                                                                                                                                                                               <  1:82863/295‑1 (MQ=255)                                         gggAAATAGTGGAGCAAGCTGCACCGGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCtt                                                                                                                                                                                                                                                               <  1:182401/295‑1 (MQ=255)                                           gAAATCGTGGAGCAAGTGGCACCTGATGAATTTTTTTGGCATCCTTAATCAGGGCTTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATTTTATTGCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTcc                                                                                                                                                                                                                                                             <  2:273227/295‑1 (MQ=21)                                           gAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCACCGTTCAAATCCCCCCCCGGCGCGTTTGTCCTTCCCCGGAGCGTGTTCCCCCTCCAACCACCGACAAAACCAAAGTCCCCGtcctcc                                                                                                                                                                                                                                                             >  2:91161/1‑295 (MQ=255)                                             gcgggagttcaggcagttcaatcaactgtttacctggtggagcgacatccaggggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGtctttc                                                                                                                                                                                                                                                             <  1:257718‑M2/141‑3 (MQ=255)                                               tattGGATCAAGGTGATCCTGATGAATGTTTGGCGGAGCCTACATCTGAGCGTACGAGGGCATTTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTGGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                                                                                                                                                                                                                                                          <  2:425807/292‑1 (MQ=21)                                               tAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACt                                                                                                                                                                                                                                                         <  1:683150/295‑1 (MQ=255)                                                   gttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa                                                                                                                                                                                                                                                       <  1:753732‑M2/147‑1 (MQ=255)                                                         gcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTAACCGACCAACAAACGGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCatt                                                                                                                                                                                                                                                 >  1:333934‑M2/143‑295 (MQ=255)                                                            gttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGt                                                                                                                                                                                                                                              >  1:315417‑M2/140‑295 (MQ=255)                                                               caatctaccgctttccttgttgctcgtcagctagcggcacgtcaccgcttgtcagcggcttacaatccggcgccaccatccagtgcaggagctccgatgctaagctggcaaacatgagaattccgatccccaattcCTGGCAGTGTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGTGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTTTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtttt                                                                                                                                                                                                                                           <  2:749760‑M2/159‑1 (MQ=255)                                                                 atcaactgtttcccttgtggagcgacatccagaggcccttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAt                                                                                                                                                                                                                                         <  1:293050‑M2/161‑1 (MQ=255)                                                                  ttatgaATTTTTTGCGCATCCTAAATCATGGCGTACGAGGGCA‑TTTTTATCGCAGTTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTg                                                                                                                                                                                                                                      <  1:125322/293‑1 (MQ=255)                                                                   gatgaATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGa                                                                                                                                                                                                                                     >  1:294844/1‑295 (MQ=255)                                                                        gtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGAtgc                                                                                                                                                                                                                                  <  1:129515‑M2/168‑2 (MQ=255)                                                                        aTTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt                                                                                                                                                                                                                                >  1:410132/1‑295 (MQ=255)                                                                             ccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCtggg                                                                                                                                                                                                                             >  1:135069‑M2/123‑294 (MQ=255)                                                                              cttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctggcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCctgggg                                                                                                                                                                                                                            <  2:86226‑M2/174‑3 (MQ=255)                                                                               gcgcATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAACGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGtt                                                                                                                                                                                                                         >  1:587636/1‑295 (MQ=255)                                                                                    tCCTAAATAAGGGCGTCCGAGGCCA‑TTGTTAGCCAAGGTAATCCATTATGTAAATTGTAGTTCGAAAAGAAAAAAGGCCT‑TCTTGCCGGTTGGCGTTTCGCTTGATTTGATGTCTGGCAGTTCATGGCGGGC‑GTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTa                                                                                                                                                                                                                    <  2:271673/295‑1 (MQ=17)                                                                                        gacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCCGCGGATTTGTCCTACTCCTGAGAGCGTTCACCGACAAACAACAGCTAAAACGAAACGCCCAAGCTCTCGTATAACCCTTTCGTTTtattttatgcctgggggatttatttc                                                                                                                                                                                                                  >  2:162709‑M2/112‑283 (MQ=255)                                                                                             agagCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCCCCCGGCGGATTTGTCCTACCCGGGAGAGTGTTCACCGACAAACCACAGGTAAAACAAAAAGCCCAGTCTTCCGGACGAACCTTTTGGTTTATTTGATTGCTGGCCTTTACCTtctccccgct                                                                                                                                                                                                           >  2:276315/1‑289 (MQ=18)                                                                                                   tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCCACGTTCCAATCCCCTCCCGGCGGATTTGGCCTACTCGGGAGAGTGTTCACCGACCACCAACCCATAAAACAAAAGTCCCAACCTTCCGACTGCGCCTTTTGCTTTATTTTATGTCTAGCAGTTCCCTTCTCTCCCCTGGGgac                                                                                                                                                                                                     >  2:243085/1‑294 (MQ=255)                                                                                                   tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCCTCTCGCATGGGgag                                                                                                                                                                                                     >  1:404774/1‑295 (MQ=255)                                                                                                   tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATggggcg                                                                                                                                                                                                     >  1:546715/1‑293 (MQ=255)                                                                                                                tttAACGCAGTGAATCCATAAATTGAATGTTCTGTCGAAAAGCAAAATGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGAGTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTAc                                                                                                                                                                                        <  2:241987/295‑1 (MQ=255)                                                                                                                    tCGCAGGTAATCCATTAATTGAATGTTATTTCGAAAGGCAAAACGGCCT‑TCCTTTCGGATGGCCTTTCGCTTGATTTTATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATc                                                                                                                                                                                    <  1:63530/295‑1 (MQ=255)                                                                                                                             aTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACC‑ATACCATCGGCGCTACg                                                                                                                                                                           >  1:701122/1‑295 (MQ=255)                                                                                                                               caaTTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCTTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGc                                                                                                                                                                         <  1:685384/293‑1 (MQ=255)                                                                                                                                   tAATTGAAAGTTAGTTCAAAAAGAAAAAAGGGCT‑GGCTTTCGGATGGTCTTTGGCTTGATTTGCTTGTTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGttt                                                                                                                                                                     <  2:427046/295‑1 (MQ=14)                                                                                                                                      ttGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTAACCACGGCGCTACGGCGGTTCAc                                                                                                                                                                  >  1:186248/1‑295 (MQ=255)                                                                                                                                              cggTTAGAAAAGAAAAAAGGCAC‑TCCTTTCGGTGGGCCTTTGGCTTGATTTGATGTCTGGCAGTGTACGGCGGGC‑GTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                                                                                                                                                          <  2:8479/293‑1 (MQ=14)                                                                                                                                               aGTTCGACAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                                                                                                                                                         >  1:447743/1‑295 (MQ=255)                                                                                                                                               aGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCCCTTCTGAGtt                                                                                                                                                         >  1:692308/1‑295 (MQ=255)                                                                                                                                                 gtCGCAAAGCCAAAAGGGCACTCCTTTGTGGTGGCTTTGTGCGTGATTTGATGTCGGGCGGGTTATGCCGGGC‑GTCCTGCCCGCAACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                                                                                                                                        <  2:547051/294‑1 (MQ=14)                                                                                                                                                              aaGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTTTGGCAGTTCCCTACTCTCGCCAGGTGACACCCCACC‑ATACCCTCGGCACCACGGCCATTTCCTTCTGTGTTCGGctacgggccagg                                                                                                                                          >  2:214584/1‑284 (MQ=14)                                                                                                                                                                  gcctgccTTTCGGCGGGCCTTTCGCTGGATTGGATGGCGGGGAGTGTATTGCGGGC‑GGCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                                                                                                                       <  2:699705/290‑1 (MQ=14)                                                                                                                                                                    a‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCCGGAGGGTTTTCCCCGACAAACCACAGATAAAACCAAAGGCCCAATCTTCCGACCGAGCCTTTTGTTTTTTTTGATGTCTGGCAGTTCCCTACCCTCTCATGGGG‑GTCCCCCCACCTACCATCGGCCCTACCGCCGTTCACTTCTGGCTTTGGCCTCGGGTCAGGTgggtc                                                                                                                                    >  2:153646/1‑293 (MQ=14)                                                                                                                                                                    a‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGAGCCGTTGCTTCGCAACGTTCCAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTCTTTGATGTCTGGCAGTTCCCTACTCTCGCCTGGGGAGGCCCCACC‑CTACCACCCGCCCTACCGGCTTTCCCTTCTTGTTTAGCCCTTGGGTCCGGTgggtc                                                                                                                                    >  2:88110/1‑293 (MQ=14)                                                                                                                                                                       ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCGGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTCTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGacca                                                                                                                                  <  1:579000/295‑1 (MQ=255)                                                                                                                                                                         tttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc                                                                                                                                >  1:279436/1‑295 (MQ=255)                                                                                                                                                                            cGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCCGGTCCCTACCCTCGCATGGGCATCCCCCACA‑CTAACCTCCGCCCTACGGCGCTTCCCTTCTTCGTTCCGCCCGGGGACCGGTggccgcaccggc                                                                                                                             >  2:304641/1‑285 (MQ=14)                                                                                                                                                                               aTGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACCCGGGAGAGTGTTCACCGACAAACCACCGATAAAACAAAAGGCCCAGTCTTCCGACCGAACCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCCCATGGGGAGACCCCACC‑CTACCCACCGCGCTACGGCGGTTCCCTTCTTGCTTCGGCCTTGGGCCCAGTGGGACCACCGCGCtc                                                                                                                          >  2:534826/1‑294 (MQ=14)                                                                                                                                                                                         cctggttttgatGTCGGGCCGTTT‑TGGTGCGCGGCCCTGCCCGCCACCCTCCGTGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTAACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAg                                                                                                                <  2:607689/289‑1 (MQ=11)                                                                                                                                                                                          cTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGa                                                                                                               >  1:331319/1‑295 (MQ=39)                                                                                                                                                                                           ttgatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACC‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTTGGCATGGGGTCAGGTGGGACCACCGCCGACCTGCCGCCAgcg                                                                                                              >  2:52161/1‑293 (MQ=255)                                                                                                                                                                                             gatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaa                                                                                                            <  1:52161/295‑1 (MQ=39)                                                                                                                                                                                                      tCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                                                                                                   >  1:701685/1‑295 (MQ=39)                                                                                                                                                                                                       cTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                                                                                                  <  1:721909/295‑1 (MQ=39)                                                                                                                                                                                                                  ttgcgtgc‑gtCCTTCCCGCCGCCCTCCTGGCCGTTGCTTCGCCACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa                                                                                       <  1:292071/293‑1 (MQ=255)                                                                                                                                                                                                                     cggtc‑gTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACGCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt                                                                                    <  1:180857/295‑1 (MQ=255)                                                                                                                                                                                                                       ggc‑gTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa                                                                                  <  1:534826/295‑1 (MQ=255)                                                                                                                                                                                                                            tCCTGCCCGCCACCCTCCCGGCCGTTGCTTCTCAACGTTCAAATCCGCTCCCTGCGGATTTGTCCTACGCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGTCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCt                                                                              <  1:436220/295‑1 (MQ=255)                                                                                                                                                                                                                                    gCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg                                                                      >  1:326789/1‑295 (MQ=255)                                                                                                                                                                                                                                       ccccTCCGGGCCTTTGCCTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtggtg                                                                   <  2:385103/294‑1 (MQ=255)                                                                                                                                                                                                                                             ccggCCGTGCCCTCGCAACGTTCAAATCCGCTCCCGGCGGATTGTCCCTCATCGGGATAGTGTTCACCGACAAACAACAGATAAACCAAAAGGCCCAGTCGTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATa                                                             <  2:186248/293‑1 (MQ=17)                                                                                                                                                                                                                                                    ttGCTTCGCAACGTTCACATCCGCTCCCGGCGGATTTGTCCTACACGGGCGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGTGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCagag                                                      <  2:294844/295‑1 (MQ=255)                                                                                                                                                                                                                                                    ttGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCagag                                                      <  1:8127/295‑1 (MQ=255)                                                                                                                                                                                                                                                       cTTCGCAACGTTCAAATCAGCGCCCGTCGGATTTGTGCTACTCGGGAGATTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCg                                                   <  2:492054/295‑1 (MQ=255)                                                                                                                                                                                                                                                        ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGa                                                  <  1:276315/295‑1 (MQ=255)                                                                                                                                                                                                                                                          cGCAACGTTAAAATCCGCTCCCGGCGGATGTGTCCTACGTGGGAGAGTGTTCACCGACAAACAACAGAGAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGGTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAAc                                                <  2:701685/295‑1 (MQ=255)                                                                                                                                                                                                                                                                    aaaCCCGCTCCCGGCGGATTGTTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGGTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTc                                      <  2:69806/295‑1 (MQ=255)                                                                                                                                                                                                                                                                      aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAATGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAc                                    >  1:532632/1‑295 (MQ=255)                                                                                                                                                                                                                                                                      aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAACGTGGTTCTGCTACCCAGAGTCGAGCTGGGGACCtacc                                    >  1:317479/1‑292 (MQ=255)                                                                                                                                                                                                                                                                       tCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACCAATTCTTTTCTCATCTGCCGAACTTTAACCTAAAACGTGGTGCTGAGTCCCAGAGTCGGCCTGGGGAccccccc                                   >  2:288965/1‑290 (MQ=255)                                                                                                                                                                                                                                                                           cTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTACAAAGTGGTGCTGATACCCAAAGTCGAACTGGGGAACtacccactt                               >  2:694088/1‑287 (MQ=255)                                                                                                                                                                                                                                                                               cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAACAGTGGTGCTGATACCCAGAGTAGAACTGGGGCCCTCACCCTTACCaa                           >  2:728836/1‑295 (MQ=255)                                                                                                                                                                                                                                                                               cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGACGAACTGGGGACCTCACCCTTACaaa                           >  1:550088/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                  cggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                        >  1:66107/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                   ggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTACAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACTCTTACCACgggg                       >  2:376360/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                                 cGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACt         <  1:288965/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        gtgtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCat  <  1:78277/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       GCACGAACCGTCGCTGACCGGGTAATTTTTATGGATCGTGGGGAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCAT  >  NZ_LN832404/3419575‑3420161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑