Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,493,618	A→G	89.4%	D35G (GAC→GGC)	nirC →	nitrite transporter NirC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,493,618	0	A	G	89.4%	134.6 / 7.0	47	D35G (GAC→GGC)	nirC	nitrite transporter NirC
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base G (20/22);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TTTCCGTCAAACATTACGAAGCGCGAGTGAAAGACGGCGTGGTGCAGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGACGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTG  >  NZ_LN832404/3493331‑3493907                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                   tttCCGTCAAACATTACGAAGCGCGAGTGAAAGACGGCGTGGTGCAGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTAt                                                                                                                                                                                                                                                                                             <  1:50288/295‑1 (MQ=255)     cGTCAAACAGTTCCAAGGGCGAGTGAAAGACGGCGTGGGTAATCGGGTCTGTTAATTGTTTAACGGTAGGCGCAATGCCTCTCCTTTTTGCATGGTCCGGTAATAATCTTCGGTATATTGCAGGACATTTGTTAAACTTTTTGTTTTATTTTTTGTTTGTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTgg                                                                                                                                                                                                                                                                                         <  2:101026/295‑1 (MQ=255)         aaaCATTACGAAGCGCGAGTGAAAGACGGCGTGGTGCAGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCt                                                                                                                                                                                                                                                                                     >  1:691606/1‑295 (MQ=255)             ttgtgaaggagggggTGAAAGATGGAGTGGGGCGGTTGCGCGGTTAATGTTTTAACGGCAGGCGCAATGCCTCCCTTTTTTGCATGGTCCTGTAATAATGTTCGGTATATTGCAGGACATTTTTTAAACGTTTTGTTTTATTTTTTGTTTTTATGTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTggg                                                                                                                                                                                                                                                                                 <  2:738446/281‑1 (MQ=255)               tACGAAGCGCGAGTGAAAGACGGCGTGGTGCAGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGat                                                                                                                                                                                                                                                                               <  1:323436/295‑1 (MQ=255)                  gAAGCGCGAGTGAAAGACGGCGTGGTGCAGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGatcat                                                                                                                                                                                                                                                                            >  1:748504/1‑295 (MQ=255)                     gcgcGAGTGAAAGACGGCGTGGTGCAGCTGCGCGGTTAATGGTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCt                                                                                                                                                                                                                                                                         <  1:226239/295‑1 (MQ=255)                                  aCGGCGTGGTGCAGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTc                                                                                                                                                                                                                                                            <  1:113805/295‑1 (MQ=255)                                              tgCTGTGCGTTTATTTTTTTCACGTGAGGCGCAATTCTTCCCCTTTTTGCATGTTCCTTTAATATTCTTCGTTATATTGCATGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTc                                                                                                                                                                                                                                               <  2:717316/294‑1 (MQ=255)                                              aGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCtg                                                                                                                                                                                                                                               >  1:678375/1‑294 (MQ=255)                                                          aaTGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTATTGGGCTCATCATGATTTTCAAGCCAGGTAA‑TTTGCTCGAACCCTCCGTa                                                                                                                                                                                                                                   >  1:622503/1‑295 (MQ=255)                                                                 tAACGGTAGGGGCAAGTCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTGTTAATCTTTTTGTTTTATTTGTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACACTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCtt                                                                                                                                                                                                                            <  2:424964/295‑1 (MQ=255)                                                                     ggggtgtgcAATGCATCTCCTTTTTGCATGGTCCGGTAATAAGTTTGGGTATATTGCAGGACATTTTTTAAACTTTTTGTTGTATTGTTTGTTTGTATTTTTTAAAGGAGAATCAAATGTTGACAGACACGATTAATAAG‑TGTGCGGCTAACGCGGCTCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGt                                                                                                                                                                                                                        <  2:512153/288‑1 (MQ=255)                                                                        agtcgcATTGCCCTCCGTTTTTGCATGGTCCTGTATTATTTTTCGGTTTATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTAGTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGAt                                                                                                                                                                                                                     <  2:55818/292‑1 (MQ=255)                                                                         ggCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATg                                                                                                                                                                                                                    >  1:666832/1‑295 (MQ=255)                                                                                   cTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGCTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCCTCCTGATTTTCACGTTCGGTCT‑TTTGATCGATCCACCCTTAAGCTCCTTGGTGacttgctacgcct                                                                                                                                                                                                          >  2:670991/1‑283 (MQ=255)                                                                                       ccTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGCCAGCTCCGCCACGGCGGACGCGTATGGGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTCGATAGATACATCCGTACGCTCTTTGGTGATGGGCGCGACCTTTgg                                                                                                                                                                                                      >  1:428130/1‑295 (MQ=255)                                                                                              gCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGGGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCCCGGTAA‑TTTGCCAGATCCATCCGTACGCCCTTTGGTGATGGGCGAGGCCTTTGGTATCgac                                                                                                                                                                                               >  2:97650/1‑293 (MQ=255)                                                                                              gCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGcc                                                                                                                                                                                               >  1:224164/1‑295 (MQ=255)                                                                                                aTGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCtt                                                                                                                                                                                             >  1:359813/1‑295 (MQ=255)                                                                                                aTGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCtt                                                                                                                                                                                             <  1:664420/295‑1 (MQ=255)                                                                                                     ccTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGACGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGc                                                                                                                                                                                        >  1:247783/1‑295 (MQ=255)                                                                                                         aaataacTTTCGGTGTTTTACAGGAAATTTTTTAAGTTTTTTGTTTAATTTTTTGTTTTAATTTTTTAAAGTATAATGAAATGTTTACAGAAACTATTAGTAAG‑TGTGCGGCTAACGCTGCACGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTACTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGt                                                                                                                                                                                    <  2:306050/294‑1 (MQ=255)                                                                                                           gttgtCTTCGTTTTATTTCAGGATATTTTTTCTACTTTTTGTTTTATTTTTTGTTTTTTGTTTTTAAAGGATAATCAAATGGTTACAGACGCTATTAATTAG‑TGTGCGGCTAACGCTTCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGACGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGa                                                                                                                                                                                  <  2:85521/291‑1 (MQ=255)                                                                                                           ataatCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGa                                                                                                                                                                                  >  1:46449/1‑295 (MQ=255)                                                                                                              atCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTa                                                                                                                                                                               >  1:352132/1‑295 (MQ=255)                                                                                                                     gattatgtattgtcATTTTTTAAACTTTTTGGTTGTGTTTTTGTTTTTATTTTTGAAAGGATAATAGAACGTGTACAGCCACTATTAAGAAG‑TGTGGGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCgg                                                                                                                                                                        <  2:342216/282‑1 (MQ=255)                                                                                                                        tatTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTc                                                                                                                                                                     <  1:539911/295‑1 (MQ=255)                                                                                                                           tGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGa                                                                                                                                                                  <  1:71392/295‑1 (MQ=255)                                                                                                                             cAGGTCATTTTTTAAACTTTTTGTTTTATTTGTTGTTTTTATTTTATAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAAc                                                                                                                                                                <  1:534101/295‑1 (MQ=255)                                                                                                                              aGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTTATTATCGCCGGTTCTGAACt                                                                                                                                                               >  1:102383/1‑295 (MQ=255)                                                                                                                              aGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCCCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGCTCCTCCTGATTTTCACGCTCGGTAA‑TTTTCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCTACCTTTGGTTTCGCCTTTACGCTGTTCATTATCGCCGGTTCTGAACt                                                                                                                                                               >  2:235015/1‑295 (MQ=255)                                                                                                                                 aCATTTTTTATACTTTTTGTTTTATTTTTTGTTTTTATTTTGTAAAGGATAATCAAATGTTTACAGACACTATGAATAAG‑TGTGCGGCTAACGCTTCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGACGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGtt                                                                                                                                                            <  1:716632/295‑1 (MQ=255)                                                                                                                                 aCATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTTTTTTTTAAAGAAAATTCAATTTTTTA‑AGGCACTTTTTATTAGTTTTCCGCCTAACGTTGCCCCCATTCCACCCCTGCCGTCAATTACCCGCCTCGCCTTTTGGGTCGTCCCCGCCATGCCGGCCGCGATGGTGGGTCTTGGGATCTCCCTGATTTTCCCTCT‑GGTAATTTTGCTCAATCCACCCTTACACCTTTTGGTGTGGGGCTCTCCTTTTGTTATCTCCTTACCTCGTGTGTTCAT‑GCCGGTTCttcaacgttc                                                                                                                                                           >  2:94955/1‑286 (MQ=255)                                                                                                                                                  tGTTTTAGTTGTTGGTGTTATTGTGTAAGGGGTAATCAAATGTTTACAGGAATTCGTATGATG‑GGTGCGGCTAGCGCTGCGCGCATTGCACGCCTGTAGGCAAATAAGCCGCTCGGGTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTGCTGAACTGTTCACCGGACACACCATGt                                                                                                                                           <  2:522108/295‑1 (MQ=255)                                                                                                                                                      ttATTTTTTTTTTTTATTTTATAAGGGATAATCAAATGTTCACGGTAACGATGAATAAG‑TTGGCGGCTATCGCTGCGCGGATTGCACGTCTGTCGGCAAATAACCCGCTCTGCTTTTGGGTCAGCTCCGCCATGGCGGACGCGTATGTGGGTCTTGTGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCt                                                                                                                                       <  2:487241/295‑1 (MQ=255)                                                                                                                                                            tttGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTACCGCTGGTTATTATCGCCGGTTCTGAACGTTTCACCGGTCACACCCTGTTCCTctcatt                                                                                                                                 >  2:106871/1‑290 (MQ=255)                                                                                                                                                                tttttATTTTTTAAATGATAATCAAATGTTTACAGAAACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTggg                                                                                                                             <  1:589315/295‑1 (MQ=255)                                                                                                                                                                   ttATTTGTTAAAGGATAAGCAAATGTTTACAGACACAATTACTCAG‑CTGGCGGCTAACGCTGCGGGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTa                                                                                                                          <  2:382452/295‑1 (MQ=255)                                                                                                                                                                                gATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGgcagca                                                                                                             >  1:209124/1‑295 (MQ=255)                                                                                                                                                                                          tGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAACTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACgg                                                                                                   <  1:239086/295‑1 (MQ=255)                                                                                                                                                                                                  gACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGAGTATGTGGGTCTTGGGATCATCCTGATTCTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACCCCCTTTGGTTATGGGCGCGACCTTTGGTATCGCCTTAACGCTTGTGATTATCGCCGGTTCTCAACTGTTCACCGGACGCACACTGTTTCTCACCTTTCGGGTCAAAGCGTGCCTCATCATCCAcccgccactct                                                                                           >  2:262092/1‑285 (MQ=255)                                                                                                                                                                                                            gatagttgggCGGTTATCGTTGCGGGCATTGCCAGCCTGTCGGCAAATAACCCGCGTGGCTTTTGTGTCAGCTCCGCCATGGCGGGCGCGTATGTTGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATcc                                                                                  <  2:565148/286‑1 (MQ=255)                                                                                                                                                                                                            ataaG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCt                                                                                 >  1:75733/1‑295 (MQ=255)                                                                                                                                                                                                                  tgtgCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTTGTATCGCCTTAAAGCTGTTGATTATCGCCGGTCCTGAACTGGTCATCGGACACACCATGTTTCTCACCTTTGGGGTAACAGCTGGCAGCATGCGCCACGGGCATATGCGGGCAAGCGcgccgc                                                                            >  2:321122/1‑295 (MQ=255)                                                                                                                                                                                                                        gCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAAGAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACct                                                                      <  1:291186/295‑1 (MQ=255)                                                                                                                                                                                                                          tAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACctgg                                                                    >  1:348396/1‑295 (MQ=255)                                                                                                                                                                                                                                  ggcgcCTTGCACGCCTTTCGGAAAGTATCCGCCTCTGCTTTGGGGTCTGCTCCGCCATGGCGGACGCTTAAGTGTGTCTTTGGGTCATCCTGATTTTCCCGCTCGGTTA‑TTTGCTCGAACCATCCTTACTCCCTTTAGTTATTGGCTCGACCTTTGGTATCGCCTTGACGCTTGTGATTATCTCCGGTTCTGAACTATTCACCGAACACACCATGTTCCTCGCCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTACCGCAAACCTGGCTGGGTaa                                                            <  2:428130/294‑1 (MQ=255)                                                                                                                                                                                                                                  cgcgctgtccctgCCTGTCGGCCAGTAACGCGCTCGGCTTTTGGGTCGGCTCCGCCTTGGCGGGTGCGTATGTGGGTCTTGTGTTCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTaa                                                            <  2:78953/283‑1 (MQ=255)                                                                                                                                                                                                                                   gcgcATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAAc                                                           >  1:324451/1‑295 (MQ=255)                                                                                                                                                                                                                                                     gggaaaTAACCCTGTCGGCGTTTTGGTCAGTTCCGCCATGGCGGGGGCGTATGTGTGGCTTGGGATCATCCTTATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATGATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTc                                         <  2:102383/292‑1 (MQ=255)                                                                                                                                                                                                                                                                      gCTTTTGGGTCAGCTCTGCCTTTGCGGGCGCGTATGTGGGTCTGTGGATCATCCTGATTTTCACTCTCGGTAA‑TTTGCTCGTTCCATCCGTACGCCCTTTGGTGATGGGCTCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAg                        <  2:175512/295‑1 (MQ=255)                                                                                                                                                                                                                                                                       cTTTTGGGTCAGCTCCGCCATGGCGGGCGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGc                       <  1:97650/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ggcgggcgCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCctgctg  <  1:38599/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                   TTTCCGTCAAACATTACGAAGCGCGAGTGAAAGACGGCGTGGTGCAGCTGCGCGGTTAATGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATAATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAG‑TGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTTTTGGGTCAGCTCCGCCATGGCGGACGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAA‑TTTGCTCGATCCATCCGTACGCCCTTTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTG  >  NZ_LN832404/3493331‑3493907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑