Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,894,419	A→G	76.5%	I134M (ATA→ATG)	EK12AG100_RS19335 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,894,419	0	A	G	76.5%	111.2 / 29.9	58	I134M (ATA→ATG)	EK12AG100_RS19335	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (4/9);  new base G (30/14);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 2.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

TGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGT  >  NZ_LN832404/3894127‑3894708                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   tGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGTTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGCGTGGTGATGAg                                                                                                                                                                                                                                                                                                  >  1:237069/1‑295 (MQ=255)           ggTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCAtt                                                                                                                                                                                                                                                                                        <  1:157546/295‑1 (MQ=255)                   taCATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCt                                                                                                                                                                                                                                                                                >  1:284424/1‑295 (MQ=255)                           aTTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTTCTGGCAGGTTTGGTGATCAGCAGAATCATTACCCGTCTACCCGGAt                                                                                                                                                                                                                                                                        >  2:772629/1‑295 (MQ=255)                           aTTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGAt                                                                                                                                                                                                                                                                        >  1:603453/1‑295 (MQ=255)                                 ctctTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGata                                                                                                                                                                                                                                                                  >  1:776142/1‑295 (MQ=255)                                           cGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACACGTCTAACCGGATTGGATATTTAATGTcc                                                                                                                                                                                                                                                        >  1:20942/1‑295 (MQ=255)                                              tAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGa                                                                                                                                                                                                                                                     >  1:664904/1‑295 (MQ=255)                                                                    gttgttAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAATTTAATCg                                                                                                                                                                                                                               >  1:595038/1‑295 (MQ=255)                                                                      tgttAACCGGTGTAATGGTGGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCggg                                                                                                                                                                                                                             <  1:376535/295‑1 (MQ=255)                                                                          aaCCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGAACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATAc                                                                                                                                                                                                                         <  1:13057/295‑1 (MQ=255)                                                                           aCCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACt                                                                                                                                                                                                                        <  1:712864/295‑1 (MQ=255)                                                                            gcGGTTTAATGTTTGCCTGTCTGCCTGAAAATATCTGTTCACAGAAACTTTATCGTTTTCTGGCTGGTTGCATATGTTGCTTCGTTATCACCTAGTTCGATTGTTCTGTTATTGTACATATTAATGTCATGTCTTCCTCTGAGTTTTTTGAAAACATTCTTTCTGTTTACAGTCTCGTTTTCCG‑CATCATTCCTGCATTGCTGGCATGTTTTGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACATACTCTTTAAGTTAAGCGTTATACtt                                                                                                                                                                                                                       <  1:433385/294‑1 (MQ=255)                                                                            ccGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTACGTTAAGCGGGATACtt                                                                                                                                                                                                                       >  1:518633/1‑295 (MQ=255)                                                                            ccGGTGTAATGGTTGCCTGTCGGCCGGAAAAGAGCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGGTATCACCGAGATCGATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACtt                                                                                                                                                                                                                       <  1:433369/295‑1 (MQ=255)                                                                               gtgtAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATCTTAAGGGCATGGCTTCCTCGGAGTTTTTTGAAAACATTCTTTCTGGTGACCGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATACGCAGAATCATTACCCGTCTACCCGGATTGTATTTTTCCTGTCCTGAAACAAACTCTTTAAGTTAAGCggcttcacttat                                                                                                                                                                                                                    >  2:561892/1‑285 (MQ=255)                                                                                          gCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACCGACTCTTTACGTTAAGGGGGATACTTTATCTTTTGGCTAc                                                                                                                                                                                                         >  2:622430/1‑295 (MQ=255)                                                                                               tCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCaaa                                                                                                                                                                                                    <  1:328049/295‑1 (MQ=255)                                                                                                          aGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGa                                                                                                                                                                                         >  1:766359/1‑295 (MQ=255)                                                                                                           gATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAATCAGACAGGAt                                                                                                                                                                                        >  2:325076/1‑295 (MQ=255)                                                                                                                 gTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCt                                                                                                                                                                                  >  1:150663/1‑295 (MQ=255)                                                                                                                  tCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTa                                                                                                                                                                                 >  1:41908/1‑295 (MQ=255)                                                                                                                   cacaGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTAt                                                                                                                                                                                >  1:498490/1‑295 (MQ=255)                                                                                                                       aaaaCGTTATCGGTGTTTGGTTGTTGGCATAGAGGGTGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACt                                                                                                                                                                            <  2:262901/294‑1 (MQ=255)                                                                                                                        aaaCGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGCCAGGATGTTTCTATGACTc                                                                                                                                                                           >  1:290470/1‑295 (MQ=255)                                                                                                                             ttATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATCCTTTATCTGTGGTCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAat                                                                                                                                                                      >  2:527910/1‑295 (MQ=255)                                                                                                                                tCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATc                                                                                                                                                                   <  1:622430/295‑1 (MQ=255)                                                                                                                                     gTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGcc                                                                                                                                                              <  1:67128/295‑1 (MQ=255)                                                                                                                                                         ggagccttgttCACCGGGATATATTGTGAAGTGATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGCGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATatc                                                                                                                                          <  2:237069/285‑1 (MQ=255)                                                                                                                                                            gtcgtTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATatcatc                                                                                                                                       <  1:728125/295‑1 (MQ=255)                                                                                                                                                               gtTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAACAAGACTCTTTACGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCGGACAGGAAGTTTCAAGGACGCAAATAATACGGCAGGTACCCAAACTCCAataactaccac                                                                                                                                    >  2:709927/1‑287 (MQ=255)                                                                                                                                                               gtTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCa                                                                                                                                    >  2:38347/1‑295 (MQ=255)                                                                                                                                                                 tATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATTGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAg                                                                                                                                  <  1:586281/295‑1 (MQ=255)                                                                                                                                                                          gaTCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGaa                                                                                                                        <  1:329286/295‑1 (MQ=255)                                                                                                                                                                             cTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGTTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATAACTCAAAATATCCGGCCGTTAACCCAATCCACATCTAATCACAATC‑CACTGAAAACa                                                                                                                     >  2:569118/1‑295 (MQ=255)                                                                                                                                                                               atttggCAGTTATTGTACATATTAAGGGCATGGTTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAgg                                                                                                                   <  2:164500/290‑1 (MQ=255)                                                                                                                                                                               atgttgtaGTTATTGTAAATATTAAGGTGATGGCTTTCTCTGTGTTGTTTGAAAACAGTCTTTCTGGTGACAGGTTCGTTGTCCG‑CATCATTCCTGCATTTCTGGCAGGTGTGGTGATGATCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAgg                                                                                                                   <  2:578298/288‑1 (MQ=255)                                                                                                                                                                                 tggtcAGTTATTTTAATTATTAAGGGCATGGTTTCCTCGGAGTTTGTTGAAAACATTTTTTCTGGTGACAGTCTCTTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGgcg                                                                                                                 <  2:776142/291‑1 (MQ=255)                                                                                                                                                                                   tgCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCG‑TACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCAt                                                                                                              <  1:82654/295‑1 (MQ=255)                                                                                                                                                                                              tACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGAATCAAAATATAAGGCCGTTACCCCAATTCAAATATTCTCCCAACC‑CACTGGAAACAGGCGCCTTTGAAAAGGa                                                                                                    >  2:648299/1‑295 (MQ=255)                                                                                                                                                                                                cATATTAATGCCATTCCTTCCCCGGATTTGTTTGAATCCATTCTGTCTGTGTCCCGTCTCTTTGTCCG‑CATCATTCCTTCATGCCTGGCAGTTGTGTTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAATTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATa                                                                                                  <  2:20942/295‑1 (MQ=255)                                                                                                                                                                                                  tatTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATaaa                                                                                                >  1:104427/1‑295 (MQ=255)                                                                                                                                                                                                    ttAAGGGATTGTCTTCTTCGGAGTTGTTTTAAAACATTCTTTTGGGGTACCGT‑TCGGTGTTCGTCATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCCGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAAc                                                                                              <  2:13892/295‑1 (MQ=255)                                                                                                                                                                                                      aaGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCg                                                                                            >  1:323255/1‑295 (MQ=255)                                                                                                                                                                                                                  tCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCACAC‑GGAAACAGGCACATTTGACCAGG‑TAAAAcccgaaggagtggatt                                                                               >  2:37170/1‑281 (MQ=255)                                                                                                                                                                                                                  tCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGAtt                                                                                <  1:38347/295‑1 (MQ=255)                                                                                                                                                                                                                            tgttTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGaaca                                                                      >  1:215208/1‑295 (MQ=255)                                                                                                                                                                                                                                  aaaaCATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCAAAAC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAATGCGATGGCTGTGAACAACAGAc                                                                >  2:69413/1‑295 (MQ=255)                                                                                                                                                                                                                                             tctGGTTGCAGTTCCGTGTTCCG‑ATTCATTCCTGCATTGCTGGCAGGTTTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTttatt                                                     <  2:41908/295‑1 (MQ=255)                                                                                                                                                                                                                                                 gTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTc                                                 <  1:712769/295‑1 (MQ=255)                                                                                                                                                                                                                                                   gACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCgg                                               >  1:692995/1‑295 (MQ=255)                                                                                                                                                                                                                                                    aCAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTAAGTTTATTACTCggt                                              >  1:12162/1‑294 (MQ=255)                                                                                                                                                                                                                                                           cGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTAGGGTCCCttt                                       >  1:333422/1‑295 (MQ=255)                                                                                                                                                                                                                                                            gTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCtttt                                      <  1:554755/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ggCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtctc            >  1:144066/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                        gCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATctct           >  1:79560/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                          aGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAAAAGGATAAAACCGTAGAGTGCGATTGCTGTGAAAAACAGACGTCAGTTTATTACTCGGGTCACTTTTATTGCGTTGATGAAGTTGAAAATCTCTGt         >  1:762267/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                               tggtgATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTTCGATTGCTGTGAACAACAGACGTCCGTTTATTACTCGGGTCCCTTTTATTTCTTTGATGACGTTGAACATTTCTTTcctgt    >  2:195754/1‑292 (MQ=255)                                                                                                                                                                                                                                                                                                 gtgATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtggt  >  1:365641/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   TGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGCATAGGTGGCGTCGTTATCACCGAGATCTATTGTGCAGTTATTGTACATATTAAGGGCATGGCTTCCTCGGAGTTGTTTGAAAACATTCTTTCTGGTGACAGTCTCGTTGTCCG‑CATCATTCCTGCATTGCTGGCAGGTGTGGTGATAAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGC‑CACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGT  >  NZ_LN832404/3894127‑3894708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑