Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	4,303,694	C→T	89.6%	L193L (CTG→CTA)	alsK ←	allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	4,303,694	0	C	T	89.6%	227.0 / 11.1	76	L193L (CTG→CTA)	alsK	allose kinase
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base T (43/25);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 5.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGAGCTTATCGGC  >  NZ_LN832404/4303406‑4303978                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               aGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGcc                                                                                                                                                                                                                                                                                          >  1:443287/1‑295 (MQ=255) aGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGGCTTAGCGcc                                                                                                                                                                                                                                                                                          >  1:658278/1‑295 (MQ=255) aGCGACGTTAAAGTCAGAAGATGGTGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGcc                                                                                                                                                                                                                                                                                          <  2:586768/295‑1 (MQ=255)    gcCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGGGCCGCGGTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGAAGAAAAGGACGCGCAATGGGTGATTTCGGGGCTGGTGTTCGTACCAGGGTCGTAGCGCCAtt                                                                                                                                                                                                                                                                                       >  2:293903/1‑295 (MQ=255)         gaaaGGCAGAAGATGAGGGGGCAATAAAGGGCAGGACCTGATGCGGCAGTGGACGGAGCAGGGACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCaga                                                                                                                                                                                                                                                                                  <  2:598149/294‑1 (MQ=255)              tCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAt                                                                                                                                                                                                                                                                             >  1:743279/1‑295 (MQ=255)              tCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGGAAGTTCGGGGCTGGTTTTCGTACCAGCGTCTTAGCGCCATTCCaggccaaa                                                                                                                                                                                                                                                                             >  2:139773/1‑289 (MQ=255)                 gaagaTGGGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTgg                                                                                                                                                                                                                                                                          <  1:146077/295‑1 (MQ=255)                  aagaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAAGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGt                                                                                                                                                                                                                                                                         <  1:739264/295‑1 (MQ=255)                   agaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGtt                                                                                                                                                                                                                                                                        >  1:221630/1‑295 (MQ=255)                       gAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGAAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGCCGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCa                                                                                                                                                                                                                                                                    >  1:721203/1‑295 (MQ=255)                                aTAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGGCCGTGCCGAGTTTTCAAGCAGACTCTGGACGAAAGGGGAGGTTTCCGCATGGCCGAAAAACTAGCGCACTGGGTAATTTCGGGGCTGTTGTTTGTACCAGCGTCCCACAGCCCTCCCAGAGCCACTGGTTTCACGGCCCCCac                                                                                                                                                                                                                                                           >  2:251592/1‑294 (MQ=255)                                       gcACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCGTCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGcc                                                                                                                                                                                                                                                    <  2:154645/295‑1 (MQ=255)                                        cACGACCTGATGCGGCAGTGGACGGCGCATTTCCTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCca                                                                                                                                                                                                                                                   >  1:693483/1‑295 (MQ=255)                                            aCCTGATGCTGCAGGGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAcg                                                                                                                                                                                                                                               <  1:637747/295‑1 (MQ=255)                                            aCCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAcg                                                                                                                                                                                                                                               <  1:293903/295‑1 (MQ=255)                                                     ggCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGAACGCGCAAGTGTTAATTTCGGGGATGTTGTTCGTACCCGCGTTCTAGCGCCCATCACGGATAATTGGTTTCCAGGACCCCAGGGTGCCCCCACTCGC‑Agcgta                                                                                                                                                                                                                                     >  2:555736/1‑294 (MQ=255)                                                        aGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCAACCGCGC‑AGTGTTggg                                                                                                                                                                                                                                  >  1:36632/1‑295 (MQ=255)                                                           ggACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGACATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCACAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGCGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTTTTGGGTCa                                                                                                                                                                                                                               >  1:627762/1‑295 (MQ=255)                                                               gtcgcAGGGACTTTTGGGTCATGGCAACGGGAGTCTGGGGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATAtc                                                                                                                                                                                                                           <  2:71941/293‑1 (MQ=255)                                                               ggCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGTGGCTGTTGTTCGTACCAGCGGCTCAGCGCCATTCCAGAGCAATTGGTTTCCGGGCCCCCAGGAGTTCCCCCAGCGCAAGTGTT‑GGTCatatg                                                                                                                                                                                                                           >  2:396477/1‑294 (MQ=255)                                                                  gcAGGGACTGTTGGGTGCTGGCAACGAGGGGCTCGCGTGGGAAGGCGGGCACATTCAGCACGCCACCGCCCAGGATAACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTcc                                                                                                                                                                                                                        <  2:358621/295‑1 (MQ=255)                                                                   ctggTACTTTTGGGGCATGGCAACGGGGGTCTAGGGTGGGAAGGCGGGAATATCCATGCCGCCACCGCCCAGGATCACCGCATCGGGATCGAACGGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTccc                                                                                                                                                                                                                       <  2:351206/293‑1 (MQ=255)                                                                    aGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCa                                                                                                                                                                                                                      >  1:90648/1‑295 (MQ=255)                                                                           tttGGGTAATGGCAACGAGAGTCTCACGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAAAAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGat                                                                                                                                                                                                               <  1:91169/295‑1 (MQ=255)                                                                              gggTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATg                                                                                                                                                                                                            <  1:139773/295‑1 (MQ=255)                                                                               ggTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTGATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGa                                                                                                                                                                                                           <  1:240503/295‑1 (MQ=255)                                                                                    ggggaaCGTGAGTCTGGCGTGGGAAGGCGGGCAGATCCATCACGCAACCGCCCAGGATCACCTCGTCGGGAGCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAg                                                                                                                                                                                                      <  2:768881/291‑1 (MQ=255)                                                                                      gCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt                                                                                                                                                                                                    >  1:294717/1‑295 (MQ=255)                                                                                        aaCGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTAACAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCAACACGCGA‑AGTGTTGGGACATACACCCCAGGGGGATATTACCCAGTTct                                                                                                                                                                                                  >  2:170279/1‑294 (MQ=255)                                                                                         aCGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCCC‑AGTGTTGGGTCATATCTCCAAGGGGGATATTACCCAGTTCGc                                                                                                                                                                                                 >  1:705838/1‑295 (MQ=255)                                                                                                   cgcgTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑cc                                                                                                                                                                                      <  1:555736/295‑1 (MQ=255)                                                                                                    gcgTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCCATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTGAGCGCCATTCCAGCGCCATTGGTTTCCCGGAACACCGGCATCCCACACGCGC‑ATTGCTAGGTCATCACTCCCAACGGGATGTGACACAGCTccacccacaccacc                                                                                                                                                                                      >  2:588100/1‑282 (MQ=255)                                                                                                     cgTGGGAAGGCGGGCCTATGCTGCACGCCACCGCCCAGGGTCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCgt                                                                                                                                                                                    <  2:36632/295‑1 (MQ=255)                                                                                                       tGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACC‑CCgtgt                                                                                                                                                                                  >  1:76007/1‑295 (MQ=255)                                                                                                                           tcaCTCCACCGCCCAGGATCACCGCATCGGGATCGAACATATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCAcc                                                                                                                                                              <  1:588825/295‑1 (MQ=255)                                                                                                                           tcaCGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCCTTCCAGAGCACTTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑CGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCCAC‑CCGTGTGCACCCGCCCGCGGCGCAcc                                                                                                                                                              >  2:582709/1‑295 (MQ=255)                                                                                                                            cgtcactccGCCCATGCTTGCCGGATCTGGATCGGAGTGAGTAGTGCGGGGTGCAAGTGCCCGTGCCGCGGTTTCAAGCAGTCTCTGGACTAAAGTGTCGGTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAAGTTCGTGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCTGAGCAATTGGTTTCCAGGCACCCAGGATTGCACCACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACT‑CCGTGTGCACTCGTCCAGGGCGCACCg                                                                                                                                                             <  2:673767/288‑1 (MQ=255)                                                                                                                            caCGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACC‑CCGTGGGCCCCCGTCCACGgcggcccg                                                                                                                                                             >  1:83157/1‑290 (MQ=255)                                                                                                                                  aCCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATc                                                                                                                                                       >  1:97712/1‑295 (MQ=255)                                                                                                                                    cGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATAACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCCTCca                                                                                                                                                     >  1:544786/1‑295 (MQ=255)                                                                                                                                        catgATCACCGCTTCTGGATCGAACAGATCAATGCTTGTGGCAATGTCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGTGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACt                                                                                                                                                 <  1:398257/292‑1 (MQ=255)                                                                                                                                         aGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAAAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCGCGC‑AGTGTTGGGTCATACCTCCACGGGGGATCTGCCCCAGTTCGCCTG‑CACACCCGTTTGCCCCCCTCCCCGGCCCCCCGTTccccccacccg                                                                                                                                                >  2:279690/1‑285 (MQ=255)                                                                                                                                           gATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCg                                                                                                                                              >  1:137206/1‑295 (MQ=255)                                                                                                                                              accgccaccgggcgcGAAAAGTTTAATGCGGGGGGAAATGCCCCTTGCCGGGTTTTAAAGCAGACTTGGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGGGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc                                                                                                                                           <  2:386284/281‑1 (MQ=255)                                                                                                                                                    aTCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACACAGTTCGCCTGCCACA‑CAGTGTGCACCCGTCCCCGGCGCCCCGTCTCTCCACACCGCACACCCCATc                                                                                                                                     >  1:675559/1‑295 (MQ=255)                                                                                                                                                      cGGGATCGAACAGATGAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATccc                                                                                                                                   <  1:331058/295‑1 (MQ=255)                                                                                                                                                       gggATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCCCCGTTCATCCACCCGGCGACCCCCATcccc                                                                                                                                  >  1:265977/1‑295 (MQ=255)                                                                                                                                                         gATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGCCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCAACGTTCATCCACACTGCGAACCCCATACACGt                                                                                                                                >  1:738160/1‑295 (MQ=255)                                                                                                                                                         gATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTAGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGGCATATATCCCAGGGGGATATGGACCAGTTCGCCTGGCCCA‑CCGTGTGCACCCGTGCACGGCGCACCGTGCATCCCCACCGCGCAACCCAAccccct                                                                                                                                >  2:105340/1‑293 (MQ=255)                                                                                                                                                                      aaggttgggggCAATGTCCGGGGCGTGGTGTTAAAGCAGGTCCTGGAGAATAGGGGCTTTGTCCGAATGGGCGAAAAGATGGCGCAAGGGGTAATTTCGGTGCTGTTGTTCGTACCAGCGTCTCAGCGCCATGCCAGATCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGAAATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgcc                                                                                                                   <  2:454502/290‑1 (MQ=255)                                                                                                                                                                       aTGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgccg                                                                                                                  >  1:653582/1‑295 (MQ=255)                                                                                                                                                                                    tGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATAACTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCCCGGCGCACCGTTCGTACACACTGCCACCCCCATCCCCGCACCCAGATAGGCCCCCAGAAcacgctg                                                                                                     >  2:173909/1‑289 (MQ=255)                                                                                                                                                                                    gcgtctggtgcaggTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgttg                                                                                                     <  1:77856/282‑1 (MQ=255)                                                                                                                                                                                          gTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGACCCCCATCCCCGTACCGAGATAGGCCGCCCACACCAGTTGTTGCGt                                                                                               >  1:14485/1‑295 (MQ=255)                                                                                                                                                                                             ccGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAg                                                                                            <  1:279690/295‑1 (MQ=255)                                                                                                                                                                                             ccGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTGGCGTAAg                                                                                            >  1:325637/1‑295 (MQ=255)                                                                                                                                                                                              cgcgTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAgg                                                                                           >  1:544831/1‑295 (MQ=255)                                                                                                                                                                                                 gTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCgg                                                                                        >  1:189186/1‑295 (MQ=255)                                                                                                                                                                                                    tcacaGCAGACGTGGGACGAAAGGGGGGTTTTCCGGAGGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTTTTGTTCTTACCAGCGTCTTAGCGCCATTCCAGGGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGttt                                                                                     <  2:97712/291‑1 (MQ=255)                                                                                                                                                                                                          cAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCCCGGCACACCGTCACTCCACACTTCGAACCCCCTCCCCGCACCGACAAATCACGCCAAACCCAGTTGCTACGGAAGGCCGTTTTctcct                                                                               >  2:737926/1‑295 (MQ=255)                                                                                                                                                                                                           aGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctacta                                                                              <  1:374599/295‑1 (MQ=255)                                                                                                                                                                                                                     cGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGCTAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTCCCACGTACCAGga                                                                    >  2:745230/1‑295 (MQ=255)                                                                                                                                                                                                                       aaaGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATATCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCCCGGCGCACCGTTCATCCACACACCGAACCCCATTCACGTACCGCGCTAGGCCCCCAGCACCCGTTCTCGCGCAACCCGGTTCTCCCCTACgcctcgggtac                                                                  >  2:180747/1‑285 (MQ=255)                                                                                                                                                                                                                         aGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTTCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGt                                                                >  1:15441/1‑295 (MQ=255)                                                                                                                                                                                                                         aGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTCATACGTCCCAGGAGAGt                                                                >  1:317646/1‑295 (MQ=255)                                                                                                                                                                                                                           gggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTg                                                              >  1:336823/1‑295 (MQ=255)                                                                                                                                                                                                                            ggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGCACCGCGAAAGGCCGCAAGAACCCGTTGTTGCCGCAGGCGGTTTTGCACTACGTCACAGGAGCGttcc                                                             >  2:545781/1‑293 (MQ=255)                                                                                                                                                                                                                              cGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAg                                                           <  1:21684/295‑1 (MQ=255)                                                                                                                                                                                                                                    ccGCATGGACGAAAAGATCGCGCAATGGGAAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCAAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTCAc                                                     >  1:577844/1‑295 (MQ=255)                                                                                                                                                                                                                                            aCGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACACCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTACGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCggg                                             >  1:365012/1‑295 (MQ=255)                                                                                                                                                                                                                                               aaaaGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGACCCAGTTTTTGCGTAAGGCGGTTTTCCACTACGTCCCAGGCAGGTTGCAGGTTAACGGCGCGGGaaa                                          >  2:253416/1‑295 (MQ=255)                                                                                                                                                                                                                                                caaGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCCCCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGaaaa                                         >  1:28941/2‑295 (MQ=255)                                                                                                                                                                                                                                                   gATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACCCTGCGCACCCCATCCCCCTACCCAGATAGGCCGCCCGAACCACTGCATGCCTAAGGCGGGTTTCCACTACTCCCCAGGAGAATTCCCGGTTAACGTCGCGGGAACACta                                      >  2:490935/1‑294 (MQ=255)                                                                                                                                                                                                                                                          cattGGGTAATTGCGGGGCTGTTGTTCGTCCCGGCGTCTTAGCTCCATTCCAGGGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGTTCATATCTCCCAGGGGGTTATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGa                               <  2:294717/292‑1 (MQ=255)                                                                                                                                                                                                                                                              gggTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAAt                           <  1:253416/295‑1 (MQ=255)                                                                                                                                                                                                                                                                   aTTTCGGGTCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATGGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGc                      <  1:270597/295‑1 (MQ=255)                                                                                                                                                                                                                                                                     ttCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAATCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGt                    >  1:246640/1‑295 (MQ=255)                                                                                                                                                                                                                                                                       cGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTAt                  <  1:105340/295‑1 (MQ=255)                                                                                                                                                                                                                                                                           gCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTACGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCCGGTTCACGTCGCGGGAAAACTCAACCGGACCATTCAGCGTATTTTc              >  2:119438/1‑295 (MQ=255)                                                                                                                                                                                                                                                                            cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCg             <  1:109375/295‑1 (MQ=255)                                                                                                                                                                                                                                                                             tgctggtCGCACCAGCGTCGTAGCGCCCTTCCAGAGAAATTGTTTTCCAGGCCCCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCGCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGa            <  2:653582/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                tgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGAGCt         >  1:80112/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                       aCCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAAAACCAGTTGTTCCGTAAGGCGGTTTTATACTACGTCCCAGGAGAGTTTCAGGTTAACGTCGCGGGAAACCTCAACCGGACAATTCAGCGTATTTTCGATCTTATCgca  >  1:340849/1‑293 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               AGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGC‑AGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACA‑CCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGAGCTTATCGGC  >  NZ_LN832404/4303406‑4303978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑