| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 1,154,267 | 2 bp→CC | 100% | coding (865‑866/2544 nt) | mdoH → | glucans biosynthesis glucosyltransferase MdoH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 1,154,267 | 0 | T | C | 91.1% | 162.0 / 6.9 | 56 | L289P (TTG→CCG) | mdoH | glucans biosynthesis glucosyltransferase MdoH |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base C (28/23); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NZ_LN832404 | 1,154,268 | 0 | T | C | 91.1% | 164.4 / 6.7 | 56 | L289P (TTG→CCG) | mdoH | glucans biosynthesis glucosyltransferase MdoH |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base C (28/23); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTA > NZ_LN832404/1153984‑1154556 || ccTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGc > 1:534573/1‑295 (MQ=255) gttccctttttggttACTGTTCTTTTGGGGTTCCGCCGTATTCTGGACGGCGTTAATGGGCTTCCTTCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTc < 2:322467/281‑1 (MQ=255) gATCCTCTTTGCGGGCTGGTTTTGTTGTGTGTCCGCCGGATTCTTGACGGCGTTAATGGGCTTCCTGCAACTGATTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGAACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTc < 2:328156/295‑1 (MQ=255) ccTCTTTGCGTTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCa < 1:28274/295‑1 (MQ=255) tACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCCACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACCGTTATAACCCGGAAATCTGAGTAGCTGAGCaacacgc > 2:531859/1‑290 (MQ=255) ggTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACAATTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCtt > 1:477856/1‑295 (MQ=255) gtgtCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTa > 1:171328/1‑295 (MQ=255) gtCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATc < 1:57563/295‑1 (MQ=255) ggATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAg > 1:190581/1‑295 (MQ=255) ttCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGCGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCg > 1:460160/1‑295 (MQ=255) aatctgctcttgtttGGGCTTCCAGCAAATGCTTATTGGTCGCGATAAATACAGTAGATCTGCGTCAACAGGTGGCGATGAACCATGAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGt < 1:388807/281‑1 (MQ=255) gACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGACAAACACTTTGATGTATACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCCGAGCAAACAGCCTGGATGGAGCTTATCGCTGAAGTCGGTgga > 1:403677/1‑294 (MQ=255) cGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAgg < 1:189290/295‑1 (MQ=255) aTGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGa > 1:245269/1‑295 (MQ=255) ggCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGAttt > 1:90309/1‑295 (MQ=255) cAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGCAGTCGGTGGCGAAGATCGGaagagcacacgtc > 1:45301/1‑283 (MQ=255) gCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATcgccgccgc < 1:385460/295‑1 (MQ=255) tATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGCTATCTGCGTCGGCGAGCAAACAGCCTGGATGGAGCTTATCGATAAAGTCGGTGGCGAGAGTCAGATTGTCTATCgaccccgccct > 2:213287/1‑293 (MQ=255) aTTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTc < 1:53266/295‑1 (MQ=255) tGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTcgc < 1:441018/295‑1 (MQ=255) aaTACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTCTAACCCGGATATATGCGTCGCAGAGCAAAAAGCCTGGGTGGTGCTTAGCGCTGAAGGCGGTGGCGAAGGTCAGATTTTCTTACGCCGCCGCCGTCGGCGGGGGAcgcg > 2:431383/1‑295 (MQ=255) aCAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTTACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGACTGGATGGCACTTATCGCTGACGTCGGTTGCGAAGGGCCGGTTTTCCCTCCGCGCCTCCGTccccggtgcaagcgaaa > 2:426474/1‑283 (MQ=255) tatCGGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCgg < 1:697360/295‑1 (MQ=255) tCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCCGAGCAACAAGCCTGGATGGCGCTTATCGCTGAAGTCGGTGGCTAAGGTCAGATTTTCTATCCCCGCCCCCGTCGCCGCGTGCAGCGCAACAGCggga > 2:136465/1‑293 (MQ=255) cTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTaa < 1:482613/295‑1 (MQ=255) gTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATc < 1:522864/295‑1 (MQ=255) aaCAGGTGGGGATGAACCATTAAACCCGGAGAATCGCAGGGGGTGGATCATGCCTAGCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGAt < 2:283234/295‑1 (MQ=255) ggCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCt > 1:628426/1‑295 (MQ=255) cGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAACCACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCAGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGAATTCTGc > 1:195300/1‑295 (MQ=255) aaCCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCg > 2:365869/1‑295 (MQ=255) aTCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAg < 1:350822/295‑1 (MQ=255) aTCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAg > 2:117115/1‑295 (MQ=255) cGGCGTTGCTCATGCCTATCTGGAACGAAGACGTGAACCGTGTTTTTGCGTGCCGGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACAt < 2:612031/295‑1 (MQ=255) gTTTATCATGCCTGTCTGTAACGAATACGTGAACCTTGTTTGTGCTGGCCTGCGTGCAACGTGTGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATGTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACAtggtg < 2:776104/295‑1 (MQ=255) tATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCt < 1:156697/295‑1 (MQ=255) gTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCAGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGACAGTCCAGCTACATGGTGGTGATGGATGATGAACCGGTAAAGACCg > 1:548568/1‑295 (MQ=255) gtgtTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGCttg > 1:734788/1‑295 (MQ=255) tttttGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGAttgttt < 1:217793/295‑1 (MQ=255) gCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCCGCCAGTACAGCTACCTGGGGGTGCTGGATGCTGACTCGGTAATGACCGGGGTTGGTTGGTGct > 2:53162/1‑294 (MQ=255) ggCGTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGc < 1:754767/295‑1 (MQ=255) cTGCGTGAAACGTGGGAATAAGTAAAAGCAACCGGGAATGCCAAACACTTTGAGGTCTACATTCTTA‑GTGACAGGTATAACCCGGATATCTGCGTCGCAGGGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTgg < 2:729050/295‑1 (MQ=255) gTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGCCCGGTTGTTGTTTGTGCGGGCTGGTgcg > 2:652522/1‑295 (MQ=255) gCAACCTGTGATTCCGTAAAAGCCACCGGTAATGCCCAACACTTGTATTTCTGCATTCGTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGcc < 2:772122/295‑1 (MQ=255) aCGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGa > 1:340439/1‑295 (MQ=255) gTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTTCGCCTGATg > 1:465286/1‑295 (MQ=255) tGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg > 1:272818/1‑295 (MQ=255) gggAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGa > 1:72421/1‑295 (MQ=255) cGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCggg < 1:507255/295‑1 (MQ=255) gtaaagCCCAACACTTTG‑TGTTTGTATTGTTATGTTACATTTATACCCTTGTGATCTGCGTCGCAGAGCAAAAAGCCTGGATGGCGCTTATCTCTTAATTGGGTTGCGAAGGTCAGATTTTCTTTCGCCGCCGCCGTCGCCGCGTGAAGCGGAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGAt < 2:548568/290‑1 (MQ=255) cacTTTGATGTCTACATTCTTA‑GTGACAGATATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgtcgt > 1:571186/1‑295 (MQ=255) tgtgttttACATGCTTA‑TTGACATTGTGAACTTGGATATCGTCGTGGCAGAGCAAAAAGCCTGGATGGGGCTTATCGCTGAAGTCGGTGGCGAAGGTCTGATTTTCTATCGCCGCCGCCGTCGCCTCGTGAAGCGTAAAAGCGGTATTATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCg < 2:90309/288‑1 (MQ=255) cTACATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGCCTCGGTAATGACCGGTGATTGTTTTTGCGGGCTGGTGCGCCTGCTGGAAGCCAACCCGAACGCCGGGGTCATTCGGTCCTCGCCGaacaag > 2:689814/1‑291 (MQ=255) aCATTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGACGCCAACCCGAACGCCGGGAGCATTCAGTAGTCGCGGCAGGCGTc > 2:326533/1‑295 (MQ=255) cATGCTTA‑GTGACAGTTATACCCCGGATATCTGCTTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTcc < 1:478307/295‑1 (MQ=255) aTTCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACCGCTACATGGTGGTGCTGGATGCTGACTCGGTACTGACCGGTGATTGTTTGTGCGGGCGGGTGCGCCTGCTGGCAGCCAACGCGCACGCCGGTATCCTTCAGGCGGCGccagaacgtgtcg > 2:226894/1‑284 (MQ=255) tCTTA‑GTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTTATTGTTTGTGCGGGCTGGTGCGCCTGATTGACGGCAACCCGACAGCCGGGCTTCTTCAGTCGTCCCCGAACGTGTCCGGt > 2:735013/1‑295 (MQ=255) gTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGa < 1:365869/295‑1 (MQ=255) gTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGCTTCTTACTCGGTAATGACCGGTGCTTGTTTGTGCGGGCTGGTGCGCCTGATGGATGCCAACCCGAAAGCCGGGATCCTTACGTCTTCGCCGGACTCGTCCGGtctaggtcagct > 2:383476/1‑284 (MQ=255) ataACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGGGATTGTTTGTTCGGGCTGGTGCGCCAGGTGGAAGCCAACCCGGACGCCGGGATCATTGCGTCGCCGCCGGACGGGGCCGGTTTTGGTACGCCGtt > 2:82496/1‑294 (MQ=255) || CCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTA‑GTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTA > NZ_LN832404/1153984‑1154556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |