Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	1,638,976	C→T	21.2%	E176K (GAA→AAA)	EK12AG100_RS08165 ←	tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	1,638,976	0	C	T	21.2%	198.0 / 44.4	87	E176K (GAA→AAA)	EK12AG100_RS08165	tail fiber protein
Reads supporting (aligned to +/- strand):  ref base C (33/35);  new base T (6/11);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

CGCGCCTGCGCTTGCAGCAGTGCCGGACAGCGAGTGGGTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGC  >  NZ_LN832404/1638683‑1639265                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   cggggtcgggcgttcagcagTGCCGGACAGCGAGTGGGTATGAGCACCATCTGATGATGTCGTCCCTGCATTACTAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTcc                                                                                                                                                                                                                                                                                                   <  2:241009/281‑1 (MQ=255)         cgcTTGCAGCAGTGCCGGACAGCGAGTGGGTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCaa                                                                                                                                                                                                                                                                                           >  1:789408/1‑295 (MQ=255)            tagcggcagGGCCGGTCGGCGAGGGGGTATGAGCCCCATCTGATGATGTCTTCCCTGCATTACGAGGCTGGCCATTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATCACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATc                                                                                                                                                                                                                                                                                        <  2:699425/290‑1 (MQ=255)                aggagTGCCGGGAAGCGAGTGGGTAGGAGCACCATCTGAGGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGGTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑a                                                                                                                                                                                                                                                                                   <  1:302965/292‑1 (MQ=255)                 gcagTGCCGGACAGCGAGTGGGTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCCTTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTCTGCGCCCCGGTGTTATTCGTCGATTTGGTTCCGTACACAACCGACGTTGTGTGTTACGCCCCCTAATccttcc                                                                                                                                                                                                                                                                                   >  2:869581/1‑291 (MQ=255)                    gTGCCGGACAGCGAGTGGGTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTgg                                                                                                                                                                                                                                                                               >  1:301438/1‑295 (MQ=255)                     tGCCGGACAGCGAGTGGGTATGAGACCCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGa                                                                                                                                                                                                                                                                              <  1:52996/295‑1 (MQ=255)                             aGCGAGTGGGTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATgcgctgg                                                                                                                                                                                                                                                                      <  1:656040/295‑1 (MQ=255)                             aGCGAGTGGGTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATgcgctgg                                                                                                                                                                                                                                                                      <  1:819949/295‑1 (MQ=255)                             aGCGAGTGGGGATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATgcgctgg                                                                                                                                                                                                                                                                      <  1:695213/295‑1 (MQ=255)                              ggagctGGGTCTGAGCACCATCTGATGCGGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCTCCAAATCCGT‑ACTGGATgcgctggc                                                                                                                                                                                                                                                                     <  2:53262/290‑1 (MQ=255)                                    gggTACTAGCACCATCTTGTGATGTCTTCCCGGCCTTACGGGTCTGGCCACTACCGCTGGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCtgtg                                                                                                                                                                                                                                                               <  2:184008/295‑1 (MQ=255)                                      gTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTggg                                                                                                                                                                                                                                                             >  1:536709/1‑295 (MQ=255)                                              aCCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAt                                                                                                                                                                                                                                                     >  1:551619/1‑295 (MQ=255)                                               ccATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTTTGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGGTGTGGTTTCCGTCCACAAATCCGT‑ACTGGATGCGCTGGAGTTGTGGGTGTGCGAtt                                                                                                                                                                                                                                                    >  1:307519/1‑295 (MQ=255)                                               ccATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAtt                                                                                                                                                                                                                                                    <  1:37703/295‑1 (MQ=255)                                                 aTCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGAACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTa                                                                                                                                                                                                                                                  >  1:681841/1‑295 (MQ=255)                                                      atgatgTCTTCCCTGAATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCACCGCGCTTAGATTTGAAATAGCGGTATAACCATTAGGGAAAATGATCGTGTTCGTGCCACCAAATGCACCGGAACTCGTGTGTTGGTGCGCACTGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCACCGGTGTTATTCGTGGATTTGGTTACGTAATAAAACGAAGATGTGGTTTCCGTCGTCAAGTACGT‑ACTGAATGCGCTGGCCCTGTGGGTCTGCTGTTTCATgtg                                                                                                                                                                                                                                             >  2:352773/1‑293 (MQ=255)                                                          tgTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTcc                                                                                                                                                                                                                                         <  1:770243/295‑1 (MQ=255)                                                                cccTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTcc                                                                                                                                                                                                                                   >  1:543348/1‑295 (MQ=255)                                                                      aTTCCGAGGCTGGCCACTCCCGCTTGTTGTGCTCATAATCCCCGTGTTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGat                                                                                                                                                                                                                             <  2:789408/295‑1 (MQ=255)                                                                        tACTAGTCGGGCCCCTTCCGCTGGTGGGGCTCATAACCCCCTCGCTTAGATTGGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCTTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGataa                                                                                                                                                                                                                           <  2:589704/295‑1 (MQ=255)                                                                          cGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGataata                                                                                                                                                                                                                         <  1:884450/295‑1 (MQ=255)                                                                           gAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTTCTGAGataattc                                                                                                                                                                                                                        >  1:185085/1‑293 (MQ=255)                                                                                 ggCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCg                                                                                                                                                                                                                  <  1:894102/295‑1 (MQ=255)                                                                                      cTACCGCTGTGTGGGCTCAGAATCCCGTCGCTTAGATTTGAAATGGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCAc                                                                                                                                                                                                             <  2:872760/295‑1 (MQ=255)                                                                                             ttgttgTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCggg                                                                                                                                                                                                      <  1:843322/295‑1 (MQ=255)                                                                                               gtggtgGTCATAATCCCCGCGTGTAGATTTGAAATGGCTGTATAACTATAAGGGAAGAAGCTCGATATAGTTACAACAAATGTACCAGAACTCTTGGATTGTGGGGGACCGGAACTATTAGCTGTCCCGCTAATACTATGGGTATGCGCCTCGTTGTTATTCGTAGATTTGTTTCCGTAATCAAACGACGATGAGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCt                                                                                                                                                                                                    <  2:621385/292‑1 (MQ=255)                                                                                                 tgtgCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGTGCTTg                                                                                                                                                                                                  >  1:430162/1‑295 (MQ=255)                                                                                                      tCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGCTTTAATGCCGTCCTGTTCCTGAGATAATCGGGCACGACCACTGGAGGCCTTGCCCta                                                                                                                                                                                             >  2:764843/1‑294 (MQ=255)                                                                                                        aTAATCCCCGCGCTTAGATTTGAAATCGCTGTATAACCATTAGGGAAAATGCTCGGGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGAGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAAGACGACCCGACCACTGGCGGGCTTGCCCTTaa                                                                                                                                                                                           <  1:457326/295‑1 (MQ=255)                                                                                                                cgcgCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGCTTTAATGCCGTCCTGTTCCTGAGATACTCCGGCCCGACCACTGGCGGGCTTACCCTCACACGTCCAg                                                                                                                                                                                   >  2:573021/1‑295 (MQ=255)                                                                                                                   gcTTAGATTCGACATTGGGGTAACACCATTAGGGAGATTGCTCGTGTTCGTTCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCa                                                                                                                                                                                <  2:551619/295‑1 (MQ=255)                                                                                                                     ttAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCACAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTACTGCCGTCCTGTTCCTGAGATAATACGGCCCGACCCCTGGCGGGCTTGCCCTTCATCGTCCCGCCACg                                                                                                                                                                              >  1:404020/1‑295 (MQ=255)                                                                                                                          ttttAAATCGCGGTATAACCTTAGGGGAAAAGGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGGACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCatat                                                                                                                                                                         <  2:748535/295‑1 (MQ=255)                                                                                                                             tgaattgggttctaACCATTAGGGAACTTGCTCGGTGTCCTGCCCCCAAATGCCCCGGAACCCTTGGTTTGGTGCGCACCGGAACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAg                                                                                                                                                                      <  2:185085/283‑1 (MQ=255)                                                                                                                                    cgGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCAc                                                                                                                                                               >  1:12444/1‑295 (MQ=255)                                                                                                                                    cgGTAGAACCATTAGGGAAAATGCGCGTGGTCGTGCTACCAAATGCACCGGAACTGTTGTTTTGGTGCGCACCGGCACTAGTTGCGGTCCCGTTAATACTATGGGTATGCTCTCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACTATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCAc                                                                                                                                                               <  2:681841/295‑1 (MQ=255)                                                                                                                                      gTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGCGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGc                                                                                                                                                             >  1:765857/1‑295 (MQ=255)                                                                                                                                        ataACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCTTGAGATGATACGGCCCGACCACTGGCGGGCTTGCGCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCt                                                                                                                                                           >  1:678321/1‑295 (MQ=255)                                                                                                                                           aCCATTAGGGAAAATGCTCGTGGTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGTGCACCGGCACTATTTGCGGTCCCGCTAATACTATGTGTATGCGCCCCGGTGTTATTCGTGTATTTGGTTGCGGAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGTTGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGAc                                                                                                                                                        <  1:873549/295‑1 (MQ=255)                                                                                                                                               tttgggAAAATGCTCGGGTCCGGGCCACCGCATGCCCCGGAACTCTTGTGTTGGTGCGCACCGGCACGATTTGCGGTCCTGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGAt                                                                                                                                                    <  2:12444/292‑1 (MQ=255)                                                                                                                                                  gggAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCTAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATCATACGGCCCGACCACTGGCGGGCTTGCCCTTTATCGTCCAGACACGCCTAACAGGGACTAGGCCTGGAGGATAAg                                                                                                                                                 >  2:663562/1‑295 (MQ=255)                                                                                                                                                       aaTGCTCGTGTTCGTGCCACGAATGGGACCGGATGTCTTGTGGTGGTGCGCGCCGGCACTATTTGCGGTGCCGCTATTACTATGGGTATGCGCCCCGGTGTTATTCGTGTATTTGGTTCCGTAATCTAACGACGATGTGGTTTCCGTCCCCGAATCCGT‑ACTGGATGCGCTGGCGCTATGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACt                                                                                                                                            <  2:105627/295‑1 (MQ=255)                                                                                                                                                                   cGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCggg                                                                                                                                <  1:419856/295‑1 (MQ=255)                                                                                                                                                                     gtcaagcacaTGCACCGGAACTCTGGGGTTGGTGCGCAGCGGCACTATTTGGGGTGCCGCTAATACTTTGGGTATGCGCCGCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGGTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTa                                                                                                                              <  2:613094/286‑1 (MQ=255)                                                                                                                                                                      gCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGTTAATACGGCCCGACCACTGGCGGGCTTGCACTTACTCGTCCAGGCACGCATATCCGGGACCACGCCTGCCGGACACGCTCCTGAACGTTTCGGGTAt                                                                                                                             >  2:264735/1‑295 (MQ=255)                                                                                                                                                                           caAATGGACGGGAGCTCTGGGGTGGGTGGTCACCGGCAGTATTTGCGGGCCCGCTAATACTATGGGTATGGGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGa                                                                                                                        <  2:75189/295‑1 (MQ=255)                                                                                                                                                                            aaaTGCATCGGAATTCTTGTGTTGGTGCGAACCGGAACTATTTGGGGTCCCGCTAATACTACTGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGAt                                                                                                                       <  2:585291/295‑1 (MQ=255)                                                                                                                                                                             aaTGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCTGGGATCACGCCTGAAGGATAAGCAACTGCAAGTTTCGGGTATGCAGAtt                                                                                                                      >  1:207729/1‑295 (MQ=255)                                                                                                                                                                                  aCCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGCTTTGTCa                                                                                                                 >  1:848165/1‑295 (MQ=255)                                                                                                                                                                                      gAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAg                                                                                                             <  1:134267/295‑1 (MQ=255)                                                                                                                                                                                           ctTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGAGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATAGTCCAGCCACGACTAGCCGGGACCACGCCTGACGGATAAGCCACACAAAGTTTCGGGTATGCAGATTTGCAACAAGTATGc                                                                                                        >  2:86318/1‑295 (MQ=255)                                                                                                                                                                                              gtgtTGGTGCGCACCGTCACTAGTTGCGGTCCCGGTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGAGGTGGTTTCCGGCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGcccc                                                                                                     <  2:543348/295‑1 (MQ=255)                                                                                                                                                                                                gtTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCCCGCATATCAGGGATCACGCCTGGCGGGTAAGCCACTGCAAGTTTTGGGGAAGAGGATTTGTCACAAGTCCGcacccg                                                                                                   >  2:391148/1‑293 (MQ=255)                                                                                                                                                                                                     ggccccccggtcgtATTTGCGGTCCTGCTACTACGTTGGGTATGCGCCCCGGTGTTGTTCGTGGTTTTTGTTCCGTAGTCAAACGCGGATGTGGTTTCCGTCGCCAAATCCGT‑ACTGTATGCGCTGGCGCTGTGGGTGGGCGATTTAGTGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAAGCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCa                                                                                              <  2:720088/282‑1 (MQ=255)                                                                                                                                                                                                        gcACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAggg                                                                                           >  1:270173/1‑295 (MQ=255)                                                                                                                                                                                                               cACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCACTGAGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGACTTGTCAAAAGTCAGCCCATGCATCCGGGCATAAcc                                                                                    >  1:584758/1‑295 (MQ=255)                                                                                                                                                                                                                aCTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCa                                                                                   >  1:181916/1‑295 (MQ=255)                                                                                                                                                                                                                   gtttgtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGAc                                                                                >  1:252414/11‑295 (MQ=18)                                                                                                                                                                                                                       gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                            <  1:320091/289‑1 (MQ=18)                                                                                                                                                                                                                       gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                            <  1:563859/289‑1 (MQ=18)                                                                                                                                                                                                                        tagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGacc                                                                           >  1:710068/6‑293 (MQ=18)                                                                                                                                                                                                                                 tAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCCCGCCTGCCGGCTAAGCAACTGCAAGTTTCGGGTATGCAGATTCTTCAACAATCTGCCCCTGCACACGGGCTTACCCAGCCCGAAAGGtctcccc                                                                  >  2:473709/1‑292 (MQ=255)                                                                                                                                                                                                                                  cacaCTGTGTGTATGTGCCCCGGTGTTCTTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                 <  2:450406/292‑1 (MQ=18)                                                                                                                                                                                                                                  aaTACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                 >  1:181198/1‑295 (MQ=255)                                                                                                                                                                                                                                      ctgtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGcc                                                             <  1:123077/289‑1 (MQ=18)                                                                                                                                                                                                                                        gtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCAc                                                           <  1:601053/291‑1 (MQ=18)                                                                                                                                                                                                                                         tGGGTATGCGCTACGGTGTTATTCGGGGATTTGGTTCCGTAATCAAACGACGATGTGGTATCCGTCCCCAAATCCGT‑ACGGGATGCGCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGACCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATAACAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACg                                                          <  1:853155/295‑1 (MQ=255)                                                                                                                                                                                                                                           tgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGAAAGATTCGGGTACGCGGATTTGTCAAAGGCACGCCCCTGCATCAGGGCATAAGCAGACAGAGCGGGCTACGGTGGCCACggg                                                        >  2:540262/2‑295 (MQ=18)                                                                                                                                                                                                                                            ggaTGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGGAATCAAACGACGAGGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCATGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGa                                                       <  1:881723/293‑1 (MQ=255)                                                                                                                                                                                                                                             tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                 <  2:719929/284‑1 (MQ=18)                                                                                                                                                                                                                                             tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                 >  1:719929/1‑284 (MQ=18)                                                                                                                                                                                                                                             gttgtgcCCCGGTGTTATGCTGGTATTTCGTGCAGTAATCAAACGACTATGTGGTTTATGTCCCCAAATCTGT‑ACTGGATGCGCTGGGGCTGTTGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCATGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGAt                                                      <  2:778982/290‑1 (MQ=255)                                                                                                                                                                                                                                                   cgccGGTTTTAGTTGTGGATTGGGTTCCGTAATCAAAGGACGTTGTGGTTTCCGTCCCCAAATCCGT‑ACTTGATTCTCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCTGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                <  2:207729/293‑1 (MQ=255)                                                                                                                                                                                                                                                   ccccGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAGTCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                <  1:337688/295‑1 (MQ=18)                                                                                                                                                                                                                                                      cGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCACGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCCTCAGGGCATAACCCGACGGAACGGTATCTGATTGCCACCGGATTgggcagac                                             >  2:530580/1‑289 (MQ=18)                                                                                                                                                                                                                                                         tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATCTCAGGGATCACGCCTGACGGATAAGCAACTGCCAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGACGGACCGGTCTCTGATGTCCACGGGGTTGGTGCCCCgct                                          >  2:759117/1‑293 (MQ=255)                                                                                                                                                                                                                                                         tgtTATTCGTGGATGTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                          <  1:624734/295‑1 (MQ=18)                                                                                                                                                                                                                                                         tggtATTCGTGGATTTAGTTCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCCATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                          <  1:641686/292‑1 (MQ=18)                                                                                                                                                                                                                                                            tggtCGTGGGGTTGGTTCCGTAATCAAACGGCGATGTGGTTTCCGTCCCCAACTCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                       <  2:63647/292‑1 (MQ=255)                                                                                                                                                                                                                                                            tATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                       >  1:748749/1‑295 (MQ=255)                                                                                                                                                                                                                                                               tCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGCCGGAACGGTATCTGATGGCCACGGGGTTGGTGAACCGACTgggaa                                    >  2:415851/1‑292 (MQ=255)                                                                                                                                                                                                                                                               tCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTa                                    <  1:814240/295‑1 (MQ=255)                                                                                                                                                                                                                                                                 gTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGGCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagc                                  <  1:259196/295‑3 (MQ=18)                                                                                                                                                                                                                                                                   ggATTTAGGGCCGTAGTCGAACGACGATGTGGTTTTCGTCCCCAAA‑CGGTGGCTGGATGGGCGGGGGCTGTGGGTGTGCGATTTAAAACCGTCCTGTTCCTGAAGCAAGACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAGGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctt                                <  2:371027/295‑5 (MQ=255)                                                                                                                                                                                                                                                                     atgtGGTGCCGTAAGTAAACGACGAGGTGGTTTCCGACCCCAGAGCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAGTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTGAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActc                              <  2:848165/292‑1 (MQ=255)                                                                                                                                                                                                                                                                      tttGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActct                             >  1:206335/1‑295 (MQ=255)                                                                                                                                                                                                                                                                        tagtgccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGAGGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTGCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctttctgg                           <  1:610552/290‑10 (MQ=18)                                                                                                                                                                                                                                                                         ggTTCCGTAAGCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCa                          <  1:880980/295‑1 (MQ=255)                                                                                                                                                                                                                                                                             ccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTTCCCCTGCATCAGGGCATAACCAGACGTAACGGTATCTGATGGCCACGGGATTGGTGCGCCGATTGGGTagctttctggtggaa                      >  1:491521/1‑281 (MQ=18)                                                                                                                                                                                                                                                                               tgtataaaatgagcaTGTGGTTTGCGTCCCAAGTGCCAT‑ACTGGATTCGCTAGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATAACGCCCGACCACTGGCGTGCGTGCCCTTAATCGTCCAGCCACGCATATCAGGGATAACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAggagga                    <  2:881442/281‑1 (MQ=255)                                                                                                                                                                                                                                                                                          acgaTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATCTTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCCGATTTGTCAAAAGTCTGCCCCTGCCTCCGGGCATAACCAGACGGAACGGTATCCGCTGGCCACGGGATTTGTGCACCCACCGGCACCAAATCTGACGGAGGATGAGCCGAggg         >  2:636801/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                             aTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACTCATACCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTATGCACCTGCATCAGGTCATGAACAGACGGAACGGTATCTGATGGCCACGGGTTCGGTGCCCCCGCTGCATAAAACGTTGCTGGAGGACGCGCCGATGGGTa      >  2:106023/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                 ggTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAAATCTGCAGGAGGATGAGCCGGGGTGGAAAGc  >  1:150801/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   CGCGCCTGCGCTTGCAGCAGTGCCGGACAGCGAGTGGGTATGAGCACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGC  >  NZ_LN832404/1638683‑1639265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑