Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	1,639,067	G→A	25.7%	P145P (CCC→CCT)	EK12AG100_RS08165 ←	tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	1,639,067	0	G	A	25.7%	156.3 / 57.0	82	P145P (CCC→CCT)	EK12AG100_RS08165	tail fiber protein
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base A (6/15);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 7.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-02

TTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAG  >  NZ_LN832404/1638775‑1639360                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      ttgttgTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCggg                                                                                                                                                                                                                                                                                                     <  1:843322/295‑1 (MQ=255)   gtggtgGTCATAATCCCCGCGTGTAGATTTGAAATGGCTGTATAACTATAAGGGAAGAAGCTCGATATAGTTACAACAAATGTACCAGAACTCTTGGATTGTGGGGGACCGGAACTATTAGCTGTCCCGCTAATACTATGGGTATGCGCCTCGTTGTTATTCGTAGATTTGTTTCCGTAATCAAACGACGATGAGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCt                                                                                                                                                                                                                                                                                                   <  2:621385/292‑1 (MQ=255)     tgtgCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGTGCTTg                                                                                                                                                                                                                                                                                                 >  1:430162/1‑295 (MQ=255)          tCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGCTTTAATGCCGTCCTGTTCCTGAGATAATCGGGCACGACCACTGGAGGCCTTGCCCta                                                                                                                                                                                                                                                                                            >  2:764843/1‑294 (MQ=255)            aTAATCCCCGCGCTTAGATTTGAAATCGCTGTATAACCATTAGGGAAAATGCTCGGGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGAGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAAGACGACCCGACCACTGGCGGGCTTGCCCTTaa                                                                                                                                                                                                                                                                                          <  1:457326/295‑1 (MQ=255)                    cgcgCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGCTTTAATGCCGTCCTGTTCCTGAGATACTCCGGCCCGACCACTGGCGGGCTTACCCTCACACGTCCAg                                                                                                                                                                                                                                                                                  >  2:573021/1‑295 (MQ=255)                       gcTTAGATTCGACATTGGGGTAACACCATTAGGGAGATTGCTCGTGTTCGTTCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCa                                                                                                                                                                                                                                                                               <  2:551619/295‑1 (MQ=255)                         ttAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCACAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTACTGCCGTCCTGTTCCTGAGATAATACGGCCCGACCCCTGGCGGGCTTGCCCTTCATCGTCCCGCCACg                                                                                                                                                                                                                                                                             >  1:404020/1‑295 (MQ=255)                              ttttAAATCGCGGTATAACCTTAGGGGAAAAGGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGGACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCatat                                                                                                                                                                                                                                                                        <  2:748535/295‑1 (MQ=255)                                 tgaattgggttctaACCATTAGGGAACTTGCTCGGTGTCCTGCCCCCAAATGCCCCGGAACCCTTGGTTTGGTGCGCACCGGAACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAg                                                                                                                                                                                                                                                                     <  2:185085/283‑1 (MQ=255)                                        cgGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCAc                                                                                                                                                                                                                                                              >  1:12444/1‑295 (MQ=255)                                        cgGTAGAACCATTAGGGAAAATGCGCGTGGTCGTGCTACCAAATGCACCGGAACTGTTGTTTTGGTGCGCACCGGCACTAGTTGCGGTCCCGTTAATACTATGGGTATGCTCTCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACTATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCAc                                                                                                                                                                                                                                                              <  2:681841/295‑1 (MQ=255)                                          gTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGCGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGc                                                                                                                                                                                                                                                            >  1:765857/1‑295 (MQ=255)                                            ataACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCTTGAGATGATACGGCCCGACCACTGGCGGGCTTGCGCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCt                                                                                                                                                                                                                                                          >  1:678321/1‑295 (MQ=255)                                               aCCATTAGGGAAAATGCTCGTGGTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGTGCACCGGCACTATTTGCGGTCCCGCTAATACTATGTGTATGCGCCCCGGTGTTATTCGTGTATTTGGTTGCGGAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGTTGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGAc                                                                                                                                                                                                                                                       <  1:873549/295‑1 (MQ=255)                                                   tttgggAAAATGCTCGGGTCCGGGCCACCGCATGCCCCGGAACTCTTGTGTTGGTGCGCACCGGCACGATTTGCGGTCCTGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGAt                                                                                                                                                                                                                                                   <  2:12444/292‑1 (MQ=255)                                                      gggAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCTAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATCATACGGCCCGACCACTGGCGGGCTTGCCCTTTATCGTCCAGACACGCCTAACAGGGACTAGGCCTGGAGGATAAg                                                                                                                                                                                                                                                >  2:663562/1‑295 (MQ=255)                                                           aaTGCTCGTGTTCGTGCCACGAATGGGACCGGATGTCTTGTGGTGGTGCGCGCCGGCACTATTTGCGGTGCCGCTATTACTATGGGTATGCGCCCCGGTGTTATTCGTGTATTTGGTTCCGTAATCTAACGACGATGTGGTTTCCGTCCCCGAATCCGT‑ACTGGATGCGCTGGCGCTATGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACt                                                                                                                                                                                                                                           <  2:105627/295‑1 (MQ=255)                                                                       cGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCggg                                                                                                                                                                                                                               <  1:419856/295‑1 (MQ=255)                                                                         gtcaagcacaTGCACCGGAACTCTGGGGTTGGTGCGCAGCGGCACTATTTGGGGTGCCGCTAATACTTTGGGTATGCGCCGCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGGTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTa                                                                                                                                                                                                                             <  2:613094/286‑1 (MQ=255)                                                                          gCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGTTAATACGGCCCGACCACTGGCGGGCTTGCACTTACTCGTCCAGGCACGCATATCCGGGACCACGCCTGCCGGACACGCTCCTGAACGTTTCGGGTAt                                                                                                                                                                                                                            >  2:264735/1‑295 (MQ=255)                                                                               caAATGGACGGGAGCTCTGGGGTGGGTGGTCACCGGCAGTATTTGCGGGCCCGCTAATACTATGGGTATGGGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGa                                                                                                                                                                                                                       <  2:75189/295‑1 (MQ=255)                                                                                aaaTGCATCGGAATTCTTGTGTTGGTGCGAACCGGAACTATTTGGGGTCCCGCTAATACTACTGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGAt                                                                                                                                                                                                                      <  2:585291/295‑1 (MQ=255)                                                                                 aaTGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCTGGGATCACGCCTGAAGGATAAGCAACTGCAAGTTTCGGGTATGCAGAtt                                                                                                                                                                                                                     >  1:207729/1‑295 (MQ=255)                                                                                      aCCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGCTTTGTCa                                                                                                                                                                                                                >  1:848165/1‑295 (MQ=255)                                                                                          gAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAg                                                                                                                                                                                                            <  1:134267/295‑1 (MQ=255)                                                                                               ctTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGAGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATAGTCCAGCCACGACTAGCCGGGACCACGCCTGACGGATAAGCCACACAAAGTTTCGGGTATGCAGATTTGCAACAAGTATGc                                                                                                                                                                                                       >  2:86318/1‑295 (MQ=255)                                                                                                  gtgtTGGTGCGCACCGTCACTAGTTGCGGTCCCGGTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGAGGTGGTTTCCGGCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGcccc                                                                                                                                                                                                    <  2:543348/295‑1 (MQ=255)                                                                                                    gtTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCCCGCATATCAGGGATCACGCCTGGCGGGTAAGCCACTGCAAGTTTTGGGGAAGAGGATTTGTCACAAGTCCGcacccg                                                                                                                                                                                                  >  2:391148/1‑293 (MQ=255)                                                                                                         ggccccccggtcgtATTTGCGGTCCTGCTACTACGTTGGGTATGCGCCCCGGTGTTGTTCGTGGTTTTTGTTCCGTAGTCAAACGCGGATGTGGTTTCCGTCGCCAAATCCGT‑ACTGTATGCGCTGGCGCTGTGGGTGGGCGATTTAGTGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAAGCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCa                                                                                                                                                                                             <  2:720088/282‑1 (MQ=255)                                                                                                            gcACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAggg                                                                                                                                                                                          >  1:270173/1‑295 (MQ=255)                                                                                                                   cACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCACTGAGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGACTTGTCAAAAGTCAGCCCATGCATCCGGGCATAAcc                                                                                                                                                                                   >  1:584758/1‑295 (MQ=255)                                                                                                                    aCTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCa                                                                                                                                                                                  >  1:181916/1‑295 (MQ=255)                                                                                                                       gtttgtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGAc                                                                                                                                                                               >  1:252414/11‑295 (MQ=18)                                                                                                                           gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                                                                                                                           <  1:563859/289‑1 (MQ=18)                                                                                                                           gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                                                                                                                           <  1:320091/289‑1 (MQ=18)                                                                                                                            tagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGacc                                                                                                                                                                          >  1:710068/6‑293 (MQ=18)                                                                                                                                     tAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCCCGCCTGCCGGCTAAGCAACTGCAAGTTTCGGGTATGCAGATTCTTCAACAATCTGCCCCTGCACACGGGCTTACCCAGCCCGAAAGGtctcccc                                                                                                                                                                 >  2:473709/1‑292 (MQ=255)                                                                                                                                      cacaCTGTGTGTATGTGCCCCGGTGTTCTTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                <  2:450406/292‑1 (MQ=18)                                                                                                                                      aaTACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                >  1:181198/1‑295 (MQ=255)                                                                                                                                          ctgtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGcc                                                                                                                                                            <  1:123077/289‑1 (MQ=18)                                                                                                                                            gtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCAc                                                                                                                                                          <  1:601053/291‑1 (MQ=18)                                                                                                                                             tGGGTATGCGCTACGGTGTTATTCGGGGATTTGGTTCCGTAATCAAACGACGATGTGGTATCCGTCCCCAAATCCGT‑ACGGGATGCGCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGACCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATAACAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACg                                                                                                                                                         <  1:853155/295‑1 (MQ=255)                                                                                                                                               tgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGAAAGATTCGGGTACGCGGATTTGTCAAAGGCACGCCCCTGCATCAGGGCATAAGCAGACAGAGCGGGCTACGGTGGCCACggg                                                                                                                                                       >  2:540262/2‑295 (MQ=18)                                                                                                                                                ggaTGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGGAATCAAACGACGAGGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCATGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGa                                                                                                                                                      <  1:881723/293‑1 (MQ=255)                                                                                                                                                 tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                <  2:719929/284‑1 (MQ=18)                                                                                                                                                 tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                >  1:719929/1‑284 (MQ=18)                                                                                                                                                 gttgtgcCCCGGTGTTATGCTGGTATTTCGTGCAGTAATCAAACGACTATGTGGTTTATGTCCCCAAATCTGT‑ACTGGATGCGCTGGGGCTGTTGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCATGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGAt                                                                                                                                                     <  2:778982/290‑1 (MQ=255)                                                                                                                                                       cgccGGTTTTAGTTGTGGATTGGGTTCCGTAATCAAAGGACGTTGTGGTTTCCGTCCCCAAATCCGT‑ACTTGATTCTCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCTGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                                                                                                               <  2:207729/293‑1 (MQ=255)                                                                                                                                                       ccccGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAGTCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                                                                                                               <  1:337688/295‑1 (MQ=18)                                                                                                                                                          cGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCACGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCCTCAGGGCATAACCCGACGGAACGGTATCTGATTGCCACCGGATTgggcagac                                                                                                                                            >  2:530580/1‑289 (MQ=18)                                                                                                                                                             tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATCTCAGGGATCACGCCTGACGGATAAGCAACTGCCAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGACGGACCGGTCTCTGATGTCCACGGGGTTGGTGCCCCgct                                                                                                                                         >  2:759117/1‑293 (MQ=255)                                                                                                                                                             tgtTATTCGTGGATGTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                                                                         <  1:624734/295‑1 (MQ=18)                                                                                                                                                             tggtATTCGTGGATTTAGTTCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCCATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                                                                         <  1:641686/292‑1 (MQ=18)                                                                                                                                                                tggtCGTGGGGTTGGTTCCGTAATCAAACGGCGATGTGGTTTCCGTCCCCAACTCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                                                                                                                      <  2:63647/292‑1 (MQ=255)                                                                                                                                                                tATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                                                                                                                      >  1:748749/1‑295 (MQ=255)                                                                                                                                                                   tCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGCCGGAACGGTATCTGATGGCCACGGGGTTGGTGAACCGACTgggaa                                                                                                                                   >  2:415851/1‑292 (MQ=255)                                                                                                                                                                   tCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTa                                                                                                                                   <  1:814240/295‑1 (MQ=255)                                                                                                                                                                     gTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGGCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagc                                                                                                                                 <  1:259196/295‑3 (MQ=18)                                                                                                                                                                       ggATTTAGGGCCGTAGTCGAACGACGATGTGGTTTTCGTCCCCAAA‑CGGTGGCTGGATGGGCGGGGGCTGTGGGTGTGCGATTTAAAACCGTCCTGTTCCTGAAGCAAGACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAGGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctt                                                                                                                               <  2:371027/295‑5 (MQ=255)                                                                                                                                                                         atgtGGTGCCGTAAGTAAACGACGAGGTGGTTTCCGACCCCAGAGCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAGTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTGAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActc                                                                                                                             <  2:848165/292‑1 (MQ=255)                                                                                                                                                                          tttGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActct                                                                                                                            >  1:206335/1‑295 (MQ=255)                                                                                                                                                                            tagtgccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGAGGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTGCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctttctgg                                                                                                                          <  1:610552/290‑10 (MQ=18)                                                                                                                                                                             ggTTCCGTAAGCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCa                                                                                                                         <  1:880980/295‑1 (MQ=255)                                                                                                                                                                                 ccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTTCCCCTGCATCAGGGCATAACCAGACGTAACGGTATCTGATGGCCACGGGATTGGTGCGCCGATTGGGTagctttctggtggaa                                                                                                                     >  1:491521/1‑281 (MQ=18)                                                                                                                                                                                   tgtataaaatgagcaTGTGGTTTGCGTCCCAAGTGCCAT‑ACTGGATTCGCTAGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATAACGCCCGACCACTGGCGTGCGTGCCCTTAATCGTCCAGCCACGCATATCAGGGATAACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAggagga                                                                                                                   <  2:881442/281‑1 (MQ=255)                                                                                                                                                                                              acgaTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATCTTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCCGATTTGTCAAAAGTCTGCCCCTGCCTCCGGGCATAACCAGACGGAACGGTATCCGCTGGCCACGGGATTTGTGCACCCACCGGCACCAAATCTGACGGAGGATGAGCCGAggg                                                                                                        >  2:636801/1‑294 (MQ=255)                                                                                                                                                                                                 aTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACTCATACCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTATGCACCTGCATCAGGTCATGAACAGACGGAACGGTATCTGATGGCCACGGGTTCGGTGCCCCCGCTGCATAAAACGTTGCTGGAGGACGCGCCGATGGGTa                                                                                                     >  2:106023/1‑295 (MQ=255)                                                                                                                                                                                                     ggTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAAATCTGCAGGAGGATGAGCCGGGGTGGAAAGc                                                                                                 >  1:150801/1‑295 (MQ=255)                                                                                                                                                                                                            gTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGGCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGccc                                                                                          <  1:224281/295‑1 (MQ=255)                                                                                                                                                                                                                 gcaaTCCGG‑AGTGGGTGCGTGGGGGGTGTGGGTGTGCGATTGAAGGCCGTCCTGCTCCTGGGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGc                                                                                     <  2:301438/293‑1 (MQ=255)                                                                                                                                                                                                                         ggtcTGGATGCGCTGGCGCGGTGGGTGTGCGTGTTAATGCCGTCCTGTTCCGGCGATAATACGGCCCGACCCCTGGCGGGCTTTCCCTTAAACGTCCAGCCAGGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGt                                                                              <  2:605009/292‑1 (MQ=255)                                                                                                                                                                                                                                aTGCGCTGTGTCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAAGACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtc                                                                       <  1:264735/295‑1 (MQ=255)                                                                                                                                                                                                                                  gcgctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGGGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcgg                                                                     <  1:859721/295‑1 (MQ=255)                                                                                                                                                                                                                                    gcttgcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGTCTTGCCCTTAATCGTCCAGCCACGCATATCAGGAATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtc                                                                   <  1:86318/295‑1 (MQ=255)                                                                                                                                                                                                                                    gctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTAGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGTGATCACGCCTGACGAATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtc                                                                   <  1:869581/295‑1 (MQ=255)                                                                                                                                                                                                                                     ctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATACAACTCTGCAGGAGGCTGAGCCGAGGTGTAAAGCTGCCCCCACGGCCACCAGTTTGCgtcggtcg                                                                  >  2:595226/1‑295 (MQ=255)                                                                                                                                                                                                                                           ctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATccc                                                            >  1:29904/1‑295 (MQ=255)                                                                                                                                                                                                                                                   gtgCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTCAAGCTGCGCCCACGGCGACCAGTTTGCGCCGGTCGTATCCcgtcggtaa                                                    >  2:609423/1‑291 (MQ=255)                                                                                                                                                                                                                                                    tacGATTTAATGCCGGCCTGTCCCTGAGACACTACGGCCCGACGACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAc                                                   <  1:404928/293‑1 (MQ=255)                                                                                                                                                                                                                                                          ttAATGCCGGCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCATGCCTGACGGATAAGCAACTGCAATTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATaa                                             <  1:573021/295‑1 (MQ=255)                                                                                                                                                                                                                                                           tAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATaaa                                            <  1:636801/295‑1 (MQ=255)                                                                                                                                                                                                                                                                   tCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGAACACGCCTGACAGATAAGCCACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCCTAACCAAACGGAACGGTATCTGATGGCTACGGGATTGGTGCACCGCATGGATAAAACCCTGACGGACGACGCGCCGAGGGGTAACGCCGCGCCCCAGGACCCCAGTTTGCGTCGGTCGACTCCCGTCGTGGACGAAAACATACCGGAg                                    >  2:843097/1‑295 (MQ=255)                                                                                                                                                                                                                                                                               gaTAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGCCTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCCGCTCCCACGGCGACCAGTTTGCGTCGGTCTTCTCCCGGCGTGAACGAATAAATGCCGGACCCTGAGCAcccg                        >  2:389316/1‑293 (MQ=255)                                                                                                                                                                                                                                                                                                  tGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCGGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGAt     >  1:290430/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                     cGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAg  >  1:527509/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      TTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAG  >  NZ_LN832404/1638775‑1639360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑