Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	1,639,159	T→C	27.6%	T115A (ACT→GCT)	EK12AG100_RS08165 ←	tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	1,639,159	0	T	C	27.6%	137.5 / 48.5	75	T115A (ACT→GCT)	EK12AG100_RS08165	tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (30/24);  new base C (6/15);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 4.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

CTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACT  >  NZ_LN832404/1638869‑1639452                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                       ctTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGAGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATAGTCCAGCCACGACTAGCCGGGACCACGCCTGACGGATAAGCCACACAAAGTTTCGGGTATGCAGATTTGCAACAAGTATGc                                                                                                                                                                                                                                                                                                    >  2:86318/1‑295 (MQ=255)    gtgtTGGTGCGCACCGTCACTAGTTGCGGTCCCGGTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGAGGTGGTTTCCGGCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGcccc                                                                                                                                                                                                                                                                                                 <  2:543348/295‑1 (MQ=255)      gtTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCCCGCATATCAGGGATCACGCCTGGCGGGTAAGCCACTGCAAGTTTTGGGGAAGAGGATTTGTCACAAGTCCGcacccg                                                                                                                                                                                                                                                                                               >  2:391148/1‑293 (MQ=255)           ggccccccggtcgtATTTGCGGTCCTGCTACTACGTTGGGTATGCGCCCCGGTGTTGTTCGTGGTTTTTGTTCCGTAGTCAAACGCGGATGTGGTTTCCGTCGCCAAATCCGT‑ACTGTATGCGCTGGCGCTGTGGGTGGGCGATTTAGTGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAAGCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCa                                                                                                                                                                                                                                                                                          <  2:720088/282‑1 (MQ=255)              gcACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAggg                                                                                                                                                                                                                                                                                       >  1:270173/1‑295 (MQ=255)                     cACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCACTGAGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGACTTGTCAAAAGTCAGCCCATGCATCCGGGCATAAcc                                                                                                                                                                                                                                                                                >  1:584758/1‑295 (MQ=255)                      aCTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCa                                                                                                                                                                                                                                                                               >  1:181916/1‑295 (MQ=255)                         gtttgtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGAc                                                                                                                                                                                                                                                                            >  1:252414/11‑295 (MQ=18)                             gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                                                                                                                                                                                                                        <  1:563859/289‑1 (MQ=18)                             gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                                                                                                                                                                                                                        <  1:320091/289‑1 (MQ=18)                              tagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGacc                                                                                                                                                                                                                                                                       >  1:710068/6‑293 (MQ=18)                                       tAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCCCGCCTGCCGGCTAAGCAACTGCAAGTTTCGGGTATGCAGATTCTTCAACAATCTGCCCCTGCACACGGGCTTACCCAGCCCGAAAGGtctcccc                                                                                                                                                                                                                                                              >  2:473709/1‑292 (MQ=255)                                        cacaCTGTGTGTATGTGCCCCGGTGTTCTTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                                                                                                             <  2:450406/292‑1 (MQ=18)                                        aaTACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                                                                                                             >  1:181198/1‑295 (MQ=255)                                            ctgtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGcc                                                                                                                                                                                                                                                         <  1:123077/289‑1 (MQ=18)                                              gtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCAc                                                                                                                                                                                                                                                       <  1:601053/291‑1 (MQ=18)                                               tGGGTATGCGCTACGGTGTTATTCGGGGATTTGGTTCCGTAATCAAACGACGATGTGGTATCCGTCCCCAAATCCGT‑ACGGGATGCGCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGACCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATAACAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACg                                                                                                                                                                                                                                                      <  1:853155/295‑1 (MQ=255)                                                 tgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGAAAGATTCGGGTACGCGGATTTGTCAAAGGCACGCCCCTGCATCAGGGCATAAGCAGACAGAGCGGGCTACGGTGGCCACggg                                                                                                                                                                                                                                                    >  2:540262/2‑295 (MQ=18)                                                  ggaTGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGGAATCAAACGACGAGGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCATGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGa                                                                                                                                                                                                                                                   <  1:881723/293‑1 (MQ=255)                                                   tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                                                                                                             >  1:719929/1‑284 (MQ=18)                                                   tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                                                                                                                                                                             <  2:719929/284‑1 (MQ=18)                                                   gttgtgcCCCGGTGTTATGCTGGTATTTCGTGCAGTAATCAAACGACTATGTGGTTTATGTCCCCAAATCTGT‑ACTGGATGCGCTGGGGCTGTTGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCATGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGAt                                                                                                                                                                                                                                                  <  2:778982/290‑1 (MQ=255)                                                         cgccGGTTTTAGTTGTGGATTGGGTTCCGTAATCAAAGGACGTTGTGGTTTCCGTCCCCAAATCCGT‑ACTTGATTCTCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCTGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                                                                                                                                                                                                            <  2:207729/293‑1 (MQ=255)                                                         ccccGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAGTCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                                                                                                                                                                                                            <  1:337688/295‑1 (MQ=18)                                                            cGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCACGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCCTCAGGGCATAACCCGACGGAACGGTATCTGATTGCCACCGGATTgggcagac                                                                                                                                                                                                                                         >  2:530580/1‑289 (MQ=18)                                                               tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATCTCAGGGATCACGCCTGACGGATAAGCAACTGCCAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGACGGACCGGTCTCTGATGTCCACGGGGTTGGTGCCCCgct                                                                                                                                                                                                                                      >  2:759117/1‑293 (MQ=255)                                                               tgtTATTCGTGGATGTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                                                                                                                                                                      <  1:624734/295‑1 (MQ=18)                                                               tggtATTCGTGGATTTAGTTCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCCATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                                                                                                                                                                      <  1:641686/292‑1 (MQ=18)                                                                  tggtCGTGGGGTTGGTTCCGTAATCAAACGGCGATGTGGTTTCCGTCCCCAACTCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                                                                                                                                                                                                                   <  2:63647/292‑1 (MQ=255)                                                                  tATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                                                                                                                                                                                                                   >  1:748749/1‑295 (MQ=255)                                                                     tCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGCCGGAACGGTATCTGATGGCCACGGGGTTGGTGAACCGACTgggaa                                                                                                                                                                                                                                >  2:415851/1‑292 (MQ=255)                                                                     tCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTa                                                                                                                                                                                                                                <  1:814240/295‑1 (MQ=255)                                                                       gTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGGCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagc                                                                                                                                                                                                                              <  1:259196/295‑3 (MQ=18)                                                                         ggATTTAGGGCCGTAGTCGAACGACGATGTGGTTTTCGTCCCCAAA‑CGGTGGCTGGATGGGCGGGGGCTGTGGGTGTGCGATTTAAAACCGTCCTGTTCCTGAAGCAAGACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAGGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctt                                                                                                                                                                                                                            <  2:371027/295‑5 (MQ=255)                                                                           atgtGGTGCCGTAAGTAAACGACGAGGTGGTTTCCGACCCCAGAGCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAGTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTGAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActc                                                                                                                                                                                                                          <  2:848165/292‑1 (MQ=255)                                                                            tttGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActct                                                                                                                                                                                                                         >  1:206335/1‑295 (MQ=255)                                                                              tagtgccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGAGGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTGCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctttctgg                                                                                                                                                                                                                       <  1:610552/290‑10 (MQ=18)                                                                               ggTTCCGTAAGCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCa                                                                                                                                                                                                                      <  1:880980/295‑1 (MQ=255)                                                                                   ccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTTCCCCTGCATCAGGGCATAACCAGACGTAACGGTATCTGATGGCCACGGGATTGGTGCGCCGATTGGGTagctttctggtggaa                                                                                                                                                                                                                  >  1:491521/1‑281 (MQ=18)                                                                                     tgtataaaatgagcaTGTGGTTTGCGTCCCAAGTGCCAT‑ACTGGATTCGCTAGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATAACGCCCGACCACTGGCGTGCGTGCCCTTAATCGTCCAGCCACGCATATCAGGGATAACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAggagga                                                                                                                                                                                                                <  2:881442/281‑1 (MQ=255)                                                                                                acgaTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATCTTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCCGATTTGTCAAAAGTCTGCCCCTGCCTCCGGGCATAACCAGACGGAACGGTATCCGCTGGCCACGGGATTTGTGCACCCACCGGCACCAAATCTGACGGAGGATGAGCCGAggg                                                                                                                                                                                                     >  2:636801/1‑294 (MQ=255)                                                                                                   aTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACTCATACCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTATGCACCTGCATCAGGTCATGAACAGACGGAACGGTATCTGATGGCCACGGGTTCGGTGCCCCCGCTGCATAAAACGTTGCTGGAGGACGCGCCGATGGGTa                                                                                                                                                                                                  >  2:106023/1‑295 (MQ=255)                                                                                                       ggTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAAATCTGCAGGAGGATGAGCCGGGGTGGAAAGc                                                                                                                                                                                              >  1:150801/1‑295 (MQ=255)                                                                                                              gTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGGCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGccc                                                                                                                                                                                       <  1:224281/295‑1 (MQ=255)                                                                                                                   gcaaTCCGG‑AGTGGGTGCGTGGGGGGTGTGGGTGTGCGATTGAAGGCCGTCCTGCTCCTGGGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGc                                                                                                                                                                                  <  2:301438/293‑1 (MQ=255)                                                                                                                           ggtcTGGATGCGCTGGCGCGGTGGGTGTGCGTGTTAATGCCGTCCTGTTCCGGCGATAATACGGCCCGACCCCTGGCGGGCTTTCCCTTAAACGTCCAGCCAGGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGt                                                                                                                                                                           <  2:605009/292‑1 (MQ=255)                                                                                                                                  aTGCGCTGTGTCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAAGACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtc                                                                                                                                                                    <  1:264735/295‑1 (MQ=255)                                                                                                                                    gcgctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGGGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcgg                                                                                                                                                                  <  1:859721/295‑1 (MQ=255)                                                                                                                                      gcttgcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGTCTTGCCCTTAATCGTCCAGCCACGCATATCAGGAATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtc                                                                                                                                                                <  1:86318/295‑1 (MQ=255)                                                                                                                                      gctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTAGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGTGATCACGCCTGACGAATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtc                                                                                                                                                                <  1:869581/295‑1 (MQ=255)                                                                                                                                       ctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATACAACTCTGCAGGAGGCTGAGCCGAGGTGTAAAGCTGCCCCCACGGCCACCAGTTTGCgtcggtcg                                                                                                                                                               >  2:595226/1‑295 (MQ=255)                                                                                                                                             ctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATccc                                                                                                                                                         >  1:29904/1‑295 (MQ=255)                                                                                                                                                     gtgCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTCAAGCTGCGCCCACGGCGACCAGTTTGCGCCGGTCGTATCCcgtcggtaa                                                                                                                                                 >  2:609423/1‑291 (MQ=255)                                                                                                                                                      tacGATTTAATGCCGGCCTGTCCCTGAGACACTACGGCCCGACGACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAc                                                                                                                                                <  1:404928/293‑1 (MQ=255)                                                                                                                                                            ttAATGCCGGCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCATGCCTGACGGATAAGCAACTGCAATTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATaa                                                                                                                                          <  1:573021/295‑1 (MQ=255)                                                                                                                                                             tAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATaaa                                                                                                                                         <  1:636801/295‑1 (MQ=255)                                                                                                                                                                     tCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGAACACGCCTGACAGATAAGCCACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCCTAACCAAACGGAACGGTATCTGATGGCTACGGGATTGGTGCACCGCATGGATAAAACCCTGACGGACGACGCGCCGAGGGGTAACGCCGCGCCCCAGGACCCCAGTTTGCGTCGGTCGACTCCCGTCGTGGACGAAAACATACCGGAg                                                                                                                                 >  2:843097/1‑295 (MQ=255)                                                                                                                                                                                 gaTAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGCCTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCCGCTCCCACGGCGACCAGTTTGCGTCGGTCTTCTCCCGGCGTGAACGAATAAATGCCGGACCCTGAGCAcccg                                                                                                                     >  2:389316/1‑293 (MQ=255)                                                                                                                                                                                                    tGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCGGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGAt                                                                                                  >  1:290430/1‑295 (MQ=255)                                                                                                                                                                                                       cGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAg                                                                                               >  1:527509/1‑295 (MQ=255)                                                                                                                                                                                                         ggCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTa                                                                                             >  1:446369/1‑295 (MQ=255)                                                                                                                                                                                                               cccTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTTACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCAc                                                                                       <  1:663562/295‑1 (MQ=255)                                                                                                                                                                                                                ccTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCAcc                                                                                      <  1:759117/295‑1 (MQ=255)                                                                                                                                                                                                                  ttAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCCCCGCTTGTACACCTCCCGCCGATGacacacccaccct                                                                                    >  2:174280/1‑283 (MQ=255)                                                                                                                                                                                                                            aGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGCGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTTAACGAATAACTGCCGGAGCATGGGAACCGCTCGTACCACTCCCGCCGACGAGTACATCCCCTTccc‑ccagcgc                                                                         >  2:147540/1‑289 (MQ=255)                                                                                                                                                                                                                                     tatCAGGGATCACGCCTAACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGTAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACACTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATccc                                                                <  1:106023/295‑1 (MQ=255)                                                                                                                                                                                                                                          gggATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTCCCCCTCCCGCCGATGAGTAACTCACCATCGCACACCGACT‑TCATCCCCTTCa                                                           >  2:699374/1‑295 (MQ=255)                                                                                                                                                                                                                                          aggATAACGCCTGGCGGATAAGCAACTGGAAGTTTCCGGTATGCAGATTTTGCAAAAGGCTGCCCCTTGATTAGGGCATAAACAGACGGAACGGTATCTGATGGGCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCa                                                           <  2:270173/294‑1 (MQ=255)                                                                                                                                                                                                                                           ggATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACAGCGGTCATCCCTTTCAg                                                          >  1:80679/1‑295 (MQ=255)                                                                                                                                                                                                                                              tCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTg                                                       <  1:391148/295‑1 (MQ=255)                                                                                                                                                                                                                                                    cTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGat                                                 >  1:298418/1‑295 (MQ=255)                                                                                                                                                                                                                                                     tGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGAACAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGACGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCCGTCATCCCGCTCGGGTGAATGata                                                >  2:650863/1‑295 (MQ=255)                                                                                                                                                                                                                                                           aTAAGCAACTGCAAGTTTCGGGAATGCAGTATTATCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGGTGGCCACGGGATTGGTGCACCGACTGGATAAAACCCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTAGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCa                                          <  1:64046/295‑1 (MQ=255)                                                                                                                                                                                                                                                              aGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGTATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGCATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACAGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCACTTTCAGGCGAATGATATTTCCATaa                                       >  1:620545/1‑294 (MQ=255)                                                                                                                                                                                                                                                                 aaCTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCt                                    >  2:840173/1‑295 (MQ=255)                                                                                                                                                                                                                                                                  aCTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTg                                   >  1:265758/1‑295 (MQ=255)                                                                                                                                                                                                                                                                   cTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGACTGATATTTCCATACGCTGt                                  >  1:563803/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                     cAGGTTTGTCAAAAGTCGTCACGTGCATCAGGACAGAACCAGACGGAACGGTATCGGAGGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTtata                <  2:584758/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                     cAGATTTGTCAAAGTGCTGCCCGGGCACCAGGGCATGACCAGACGGAACGGTATCTGATGGCCACGGGATTGGGGCACCGACTGGATAAAACTCTGCAGGACGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTtata                <  2:536709/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                         tttGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACAcc            >  1:497559/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                          ttGTCAACAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCt           <  1:764843/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                               aaaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTCTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACATCGaat      >  1:565729/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                aaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTAGCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGtata     <  2:748749/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   gTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CACCGGCTGTCATCCCTTTCAGGGGAATGATATTTCCATACGCTGTTGGCTACACGTTGTTAATCAACCCGTATAACt  >  2:479409/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                       CTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGAATAAATGCCGGAGCATGAGCACCGCTTGTACCACTCCAGCCGATGAGTAACTCACCTTCGC‑CAACGGCTGTCATCCCTTTCAGGTGAATGATATTTCCATACGCTGTTGGATATCCGTTGTTATACACCTCGTATAACT  >  NZ_LN832404/1638869‑1639452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑