Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,503,761	T→C	78.5%	N30D (AAC→GAC)	EK12AG100_RS17515 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,503,761	0	T	C	78.5%	166.5 / 44.5	84	N30D (AAC→GAC)	EK12AG100_RS17515	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (6/12);  new base C (21/44);  total (27/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

ATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGC  >  NZ_LN832404/3503474‑3504048                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                 aTCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCAt                                                                                                                                                                                                                                                                                                >  1:684526/1‑290 (MQ=255) aTCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCAt                                                                                                                                                                                                                                                                                                >  1:963895/1‑290 (MQ=255)     aCCGTCAGCGCGGTCAGCATGTGGTATACGGGCAACAGGAAAGTAAGGATAAACACGCCCCAGATAGAGCCAAGACCAAACGCCAGTAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCTGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGaaa                                                                                                                                                                                                                                                                                            <  2:598063/290‑1 (MQ=255)               cgtgcgacagcTGTGTAACTGGAAACAGGCTAGTAAGGACCAACAGGCCCCAAAAAGGTCCCAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCTAGGTAATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGAAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAgccgcc                                                                                                                                                                                                                                                                                  <  2:63745/283‑1 (MQ=255)                  cagtcacacgggttACTGGCAAAAGGCAAGCAAAGATCAACACGCCCCAGATAGACCCAAGACCAACCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAg                                                                                                                                                                                                                                                                               <  2:221236/278‑1 (MQ=255)                            gTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCa                                                                                                                                                                                                                                                                     >  1:105413/1‑290 (MQ=255)                             tttACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAg                                                                                                                                                                                                                                                                    <  1:131822/290‑1 (MQ=255)                                 cTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCAATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCTGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGc                                                                                                                                                                                                                                                                <  1:406384/290‑1 (MQ=255)                                  tgacAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCg                                                                                                                                                                                                                                                               <  1:336096/287‑1 (MQ=255)                                     cagacgtgacggatggATCAGTACGCATCAGATAGCGCACAGACTAAACGCCGTCAGTGTGTTGTGCGCATGAACGCCGAGTGTATCGGTAGAAAGAAAGCGTAACTCTGCGCTGGTCTGCCCGGTTTTCTGGGAAGAAGCGGCCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATg                                                                                                                                                                                                                                                            <  2:779419/276‑1 (MQ=255)                                        cAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGACAAACAGCCGCCGAGCGAGGCGCCAGCAGCGACGTCa                                                                                                                                                                                                                                                         >  2:353776/1‑290 (MQ=255)                                        cAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTcc                                                                                                                                                                                                                                                         >  1:979591/1‑289 (MQ=255)                                           gCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATAc                                                                                                                                                                                                                                                      >  1:623517/1‑290 (MQ=255)                                            cAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCACGGTTTTCAGGGTAAAAGAGATCCCCACCGATGCCCCCAAACCAATGCTCAGACCAAAAGCGCTACTCCCCGCATCACGACGGTTCATATCGCCTTCAATCAGTTGCCGCTGGAACCGGCGGCTGACTGTATGAATAACCACCGACGAGCGCTGCGCCAGACGccgtctcatgaa                                                                                                                                                                                                                                                     >  2:53816/1‑279 (MQ=255)                                             aaGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATAccc                                                                                                                                                                                                                                                    <  1:108136/290‑1 (MQ=255)                                               gTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCACAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTTCGGCAGGCTCGGGCGGATGCCGTACTTAAAAACAACCCCCTACAGCTGCGCCAGAACAGATGtgtaacccat                                                                                                                                                                                                                                                  >  2:220836/1‑281 (MQ=255)                                                      gacacacGCCCCAGAGAGGGCCAAGACCAAAGGCCACCAGGCTTGTGATAGCCAGGACGCGGGGGAGATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGCGACCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCAc                                                                                                                                                                                                                                           <  2:467591/287‑1 (MQ=255)                                                                 ccgaaaGCGCCACTACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAATAAAGAGTACCCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCACTGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGAt                                                                                                                                                                                                                                <  1:278484/285‑1 (MQ=255)                                                                 cAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGAt                                                                                                                                                                                                                                <  1:177089/290‑1 (MQ=255)                                                                    aTAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAAt                                                                                                                                                                                                                             <  1:880522/290‑1 (MQ=255)                                                                    aTAGAGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAAt                                                                                                                                                                                                                             <  1:210895/290‑1 (MQ=255)                                                                           cAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAata                                                                                                                                                                                                                      <  1:1062474/290‑1 (MQ=255)                                                                                         ctcggggtttttAGCGCCAGGACGCCGAGGCTATCGGGAGGAAGAAAGAGTAACCCTGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGACCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGt                                                                                                                                                                                                        <  2:101883/281‑1 (MQ=255)                                                                                            aGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCCATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAACCAGCCGCCGAGCGATGCGCATGCAGCGCTGTCATACCAGTCAGACCGGCCCCCCCGATACTCAGACTCTAAATATCCACAGTctg                                                                                                                                                                                                     >  2:201892/1‑288 (MQ=255)                                                                                               cTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAATAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGcc                                                                                                                                                                                                  <  1:1063984/290‑1 (MQ=255)                                                                                               cTGTTAATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGcc                                                                                                                                                                                                  <  1:364301/290‑1 (MQ=255)                                                                                                     aTCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTAt                                                                                                                                                                                            <  1:50193/290‑1 (MQ=255)                                                                                                      tCGCCAGGACCCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTAtt                                                                                                                                                                                           <  1:243430/290‑1 (MQ=255)                                                                                                       cGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGACGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTAttt                                                                                                                                                                                          >  1:671744/1‑290 (MQ=255)                                                                                                        gCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCCCCAGAAGCGATGCCATACCAGTCAGGCACGCCACCGCGATAATCTGAATATACAGATCCATAGTCGTGCCTTTCATTT‑c                                                                                                                                                                                        >  2:442161/1‑290 (MQ=255)                                                                                                            ggACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGAt                                                                                                                                                                                    <  1:77830/290‑1 (MQ=255)                                                                                                             gACGCCGAGGAAATCGGGAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATg                                                                                                                                                                                   <  1:9123/290‑1 (MQ=255)                                                                                                                  cGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTaa                                                                                                                                                                              <  1:926657/290‑1 (MQ=255)                                                                                                                    aGGATATCGGGAGGAAGAAAGAGTAAACATGCGTTGAGCAGCCCGGGTGTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGAACAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATc                                                                                                                                                                            <  1:519937/290‑1 (MQ=255)                                                                                                                      aatatCGGTAGGAAGAAAGATTAACCATGCTTTGAGCAGCCCGGTTGTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAATCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCTATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAg                                                                                                                                                                          <  1:956767/289‑1 (MQ=255)                                                                                                                           cGGTAGGAAGAAAGAGTAACCAGGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCg                                                                                                                                                                     <  2:291198/290‑1 (MQ=255)                                                                                                                                ggAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCCCGCCACCACGATAATCTGACTATACAGAACCCAATGCGTTCCTTTTATTTCCACAT‑GTTAATCCGCCTCGGAATc                                                                                                                                                                >  2:400469/1‑290 (MQ=255)                                                                                                                                 gaagaaAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAATAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATTGGAATcg                                                                                                                                                               <  1:600790/290‑1 (MQ=255)                                                                                                                                     aaagaGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATCTAACAATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCCGCATCGGCATcgccgg                                                                                                                                                           >  2:97592/1‑287 (MQ=255)                                                                                                                                             aCCAGGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGt                                                                                                                                                   <  1:525136/290‑1 (MQ=255)                                                                                                                                               cATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATACCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGc                                                                                                                                                 >  1:780613/1‑290 (MQ=255)                                                                                                                                                aTGCGTTGAGCCGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCt                                                                                                                                                <  1:934317/290‑1 (MQ=255)                                                                                                                                                           agcCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAgg                                                                                                                                     <  1:961221/290‑1 (MQ=255)                                                                                                                                                           agcCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAgg                                                                                                                                     <  1:951149/290‑1 (MQ=255)                                                                                                                                                               ctgTTTTCAGGTTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCaa                                                                                                                                 <  1:218119/288‑1 (MQ=255)                                                                                                                                                                 gTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAAc                                                                                                                               <  1:1005494/290‑1 (MQ=255)                                                                                                                                                                      cAGGGTAAAAGAGATCCCCACCGAGGCCAAAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACAGAAAGGCCAAACGCCAc                                                                                                                          <  1:681430/290‑1 (MQ=255)                                                                                                                                                                       agtgTAAATGAGAGCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACt                                                                                                                         <  1:153460/287‑1 (MQ=255)                                                                                                                                                                       aGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACt                                                                                                                         <  1:285168/290‑1 (MQ=255)                                                                                                                                                                           caaaaagcgcccccACCGCGGCCACAAAACCCATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCGTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAACCAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTc                                                                                                                     <  2:158998/280‑1 (MQ=255)                                                                                                                                                                            aaaaGAGATCCCCCCCGAGGCAAAAAAACCAAGGCTCAGACCAAAAGCGATACTCCCCGCCCCGCGACGGTTCATATAGCCGTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTcc                                                                                                                    <  2:940385/290‑1 (MQ=255)                                                                                                                                                                               agagATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCCGAAGCGATGTCATGCCCGTCAGGCACGCCACCACTATAATCTGAATATACAGATCCACACTTCTTCCTTTTATTT‑CAGCTGGTTAATCAGCATCGGAATCGCCATTTCGCTGTGCTCAACCGAAAGGCCAACAGCCCCTTTTCCTTc                                                                                                                 >  2:322558/1‑290 (MQ=255)                                                                                                                                                                                    tCCCCACCGATGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGa                                                                                                            <  1:696790/290‑1 (MQ=255)                                                                                                                                                                                    tCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACCTGCTCAACGGACAGGCCAACCGCCACTTTTCCTTCAGCGa                                                                                                            >  2:497204/1‑290 (MQ=255)                                                                                                                                                                                     ccccACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGAt                                                                                                           <  1:733518/290‑1 (MQ=255)                                                                                                                                                                                       ccACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCCTGTTCGACGTTCTCAACGGAAAGGCCAACCGCCACTTTTCCTTCAGCCATCa                                                                                                         >  2:521763/1‑290 (MQ=255)                                                                                                                                                                                         aCCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATACATAGTCGTGCCTTGTATTT‑CAGATGGTTAATCAGCATCGGAATCGAGTGTTCGAAGGTAGCAACGGAAAGGCCAAACGCCACTTGTACTGCAGCGagccat                                                                                                       >  2:650665/1‑285 (MQ=255)                                                                                                                                                                                              gcacacaAAACAAAGGCACAGACCAAAAGCGAGACTCCCCGCCTCGCGACGTTTCATATAGCCTTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGc                                                                                                  <  2:638713/287‑1 (MQ=255)                                                                                                                                                                                              gaccAAAAAACAACTGCTAGGACCAAAAGCGATACTCCCCGCCGTGCGACGGGTCATCGAGCATTCAATCCGTGTCGGCAGGAGCGTGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGc                                                                                                  <  2:972692/288‑1 (MQ=255)                                                                                                                                                                                                 aacaAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGat                                                                                               <  1:234427/289‑1 (MQ=255)                                                                                                                                                                                                  acaAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCAACTTTTCCTTCAGCGATCATTTTGGCGata                                                                                              >  1:621620/1‑290 (MQ=255)                                                                                                                                                                                                  aaaaaaCCAACGCCCAGCCCAAAAGCGAGACTCCCCGCCCCGCGACGGTTCAGTTAGCCTTCAATCAGTGGCGGCAGGATCGGGCGGAGGCCGTCAGGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGata                                                                                              <  2:849135/288‑1 (MQ=255)                                                                                                                                                                                                   aaaaaCCAAAGCTCAGACCAAAAGCGAGACCCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCTCCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGatat                                                                                             <  2:684526/289‑1 (MQ=255)                                                                                                                                                                                                     aaaCCAATGCTACGACTAAAGCCTATACTCCCGTCCGTGCGAGGGTTCATATAGACTTCAATCAGTTGCGGCAGGATCGGGCTGAGGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGc                                                                                           <  2:750707/290‑1 (MQ=255)                                                                                                                                                                                                              ccaagcccAAAGGCGGTACCCCCCGCCTCGCGACGGGTCAGATAGCCTTCAAGCAGTTGGGGCAGGATCGGGCGGATGACGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTc                                                                                  <  2:524690/284‑1 (MQ=255)                                                                                                                                                                                                              cTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTc                                                                                  <  1:503373/290‑1 (MQ=255)                                                                                                                                                                                                                    ccAACAGCGCTGCTCCCCGCCGCGCGACGGTTCCTATGACCTTCAAGCAGTTGCGGCGGGATCGGGCGGAGCCCGTTATGAAAAACAGCCGCCGGGCGAGGCGCCAGAAGCGATGTCATACCCGTCAGGCAAGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTa                                                                            <  2:299306/290‑1 (MQ=255)                                                                                                                                                                                                                      aaaaGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAAt                                                                          >  1:149510/1‑290 (MQ=255)                                                                                                                                                                                                                      aaaaGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CCGATGGTTAATCAGCATCGGACTCGCGTGTTCGACGTGCTCAACGGACAGGCCACACGCCACTTTTCCTTCAGCCATCATTTTGGCGATATGCCCGTATTTCCCTTTAAt                                                                          >  2:121272/1‑290 (MQ=255)                                                                                                                                                                                                                      aaaaGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAAt                                                                          >  1:724654/1‑290 (MQ=255)                                                                                                                                                                                                                       aaggCGAGACGCCCCGCCTCGTGCAGGTTCATATAGCCGTCCACCAGTTGGGGCAGGAGCGGGCGGATGCCGTAATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATg                                                                         <  2:903116/287‑1 (MQ=255)                                                                                                                                                                                                                       aaaGGGATACTCCCCGCCTCGCGACGGTTCATATAGCCGTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATg                                                                         <  1:285384/290‑1 (MQ=255)                                                                                                                                                                                                                          gCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCAC‑TTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTg                                                                     <  1:162114/290‑1 (MQ=255)                                                                                                                                                                                                                           cGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTg                                                                     >  1:576133/1‑290 (MQ=255)                                                                                                                                                                                                                           cGATACTCCCCGCCTCGCGACGGTTCATATAGCCGTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTCAGTAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTg                                                                     <  1:897928/290‑1 (MQ=255)                                                                                                                                                                                                                                            cgACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCaa                                                    <  1:627565/290‑1 (MQ=255)                                                                                                                                                                                                                                            cgACGGTTCAGATAGCCTTCAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCaa                                                    <  1:790061/290‑1 (MQ=255)                                                                                                                                                                                                                                             gACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATCTGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAAc                                                   >  1:394822/1‑290 (MQ=255)                                                                                                                                                                                                                                             gACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAAc                                                   >  1:813477/1‑290 (MQ=255)                                                                                                                                                                                                                                                ggTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACttt                                                >  1:819668/1‑290 (MQ=255)                                                                                                                                                                                                                                                            cTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTa                                    >  1:985557/1‑290 (MQ=255)                                                                                                                                                                                                                                                            cTTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTa                                    <  1:353776/290‑1 (MQ=255)                                                                                                                                                                                                                                                                   cAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGTCCGTCTTTCGCTTTACTGCCTTGTTTGGCAATGGTGCCACTTTTGTTATTACCTATCCCcgc                             >  2:14860/1‑290 (MQ=255)                                                                                                                                                                                                                                                                          ggCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCa                      >  1:599200/1‑290 (MQ=255)                                                                                                                                                                                                                                                                           ccAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCaa                     <  1:85895/289‑1 (MQ=255)                                                                                                                                                                                                                                                                             aGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGAGGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATc                   <  1:3183/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGc           <  1:97592/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGc  >  1:152927/1‑290 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                 ATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTT‑CAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGC  >  NZ_LN832404/3503474‑3504048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑