| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 4,168,395 | C→A | 26.3% | intergenic (‑68/‑135) | EK12AG100_RS25840 ← / → EK12AG100_RS20635 | paraslipin/UDP‑N‑acetylmuramate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 4,168,395 | 0 | C | A | 26.3% | 128.4 / 62.3 | 88 | intergenic (‑68/‑135) | EK12AG100_RS25840/EK12AG100_RS20635 | paraslipin/UDP‑N‑acetylmuramate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (39/26); new base A (10/13); total (49/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01 | |||||||||||
TTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATG > NZ_LN832404/4168108‑4168683 | tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCAGGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCAt < 1:33606/290‑1 (MQ=17) ttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGccta > 1:556749/1‑287 (MQ=17) gaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgccataaactgccaggaattaattccCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCCGGCatt < 1:595915‑M1/177‑2 (MQ=255) gCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTCGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGCGGGTGGCGGGCAGGCACG‑CCGCCATAAACTGCCAGGCaacca > 2:44727/1‑286 (MQ=12) gcgcGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGc > 1:149560/1‑290 (MQ=32) tCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGAc > 1:125839/1‑290 (MQ=32) cctagACCCGAGGCCGAACCCGAAAGTGGAAGGCGGTAGGTCCGATGGGGGGGT‑GGGGTCTCCCGAGTGGGGAGTAGGAAACGGCCAGGAATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTGGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGAt < 2:431629/286‑1 (MQ=11) aCCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGAt < 1:915293/290‑1 (MQ=32) gCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCCAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCg > 1:800643/1‑290 (MQ=17) aGAAGTGAAACGCCGTAGCGCCGAAGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTaaca > 1:512613/1‑287 (MQ=21) aaGGGAAAGGCTGTAGCGGCGA‑GGTTGGGTGGGGGTGTCCCCATGGGAGGGAAGGAAACTGCCAGGAATCAAATAAAACGAAAGGCTCAGTAGAAAGACTGGGCTTTTCGTTTTAGCGGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAAc < 2:932616/290‑1 (MQ=11) aagccgcggtggtacGATGGAAGGGT‑GGGTGACCCTCATGCGAGTGTCGGGAACTGCCTGGAAGCAAATAAACCGAAAGGCTCAGTCGAAAGACTGGGCCTTTTGTTTTATCGGTTGTTTGTCGTTGAACGCGCTCTGGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTc < 2:363712/276‑1 (MQ=11) gcCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCctat > 1:388548/1‑290 (MQ=255) gcCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTACTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCACGCCATAAACTGCCAGGCATCAAAGTAAGCAGAAGGCCATCCTAACGGATGGCATTTTTGCGTTTCTACACACCTTTCCTTTCCTCatca > 2:264617/1‑288 (MQ=11) ctcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTCAGAAGCAACGGCACGGCGGGTGGCGGGCAGG‑ACTCCCGCCATAACCTGCCCGGCACCAAATTAAGCAGCAAGACCTCCTGACGAATGGCCTTTTTGCTTTTCTACAAACTCCTCCCTTCGTCATTTCTCCCACCCCTcacccccc > 2:1046640/1‑288 (MQ=11) gagaGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCCGACGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTCGTCAACTCTCCCAGCCCTCCCCCCCCAGCTCCGGTa > 2:714251/1‑290 (MQ=14) aattcccaattccccaggcatcaaataaAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaa < 1:39159‑M2/277‑99 (MQ=255) tAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCCACCTTACGGAAGGCCTTTTTGCGTTTCTACCAACTCTTCCTTTCCTCATTCACACAATCCATCCCCCCACCAATCCCGTCAACTa > 2:456962/1‑290 (MQ=14) gggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCCTATCTACAAGCCATCCACCCACAGATAAGGTAACCTCGc > 1:219871/1‑290 (MQ=255) aattccccaggcatCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATTAAttccccaggcatcaaataaaacgacaggctcagtcgaacgactgggcatttcgttttatctgttgtttgtcggtgaacgctctccttagtaggacaactccgccg < 1:960768‑M2/284‑106 (MQ=255) aattccccaggcatCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttagttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg < 1:622759‑M2/284‑106 (MQ=255) cAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcgg > 1:878950‑M2/1‑178 (MQ=255) cAGGCATCAAATAAAACGAAAGGCCAAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcgg > 1:417279‑M2/1‑178 (MQ=255) aGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaacgactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggc < 1:562332‑M2/290‑114 (MQ=255) aaTAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCCGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACCCTTCATGTCGTCATCTCTACAAGCCATCCCCCCACAGATACGGTAACCTTGCCTCGTTTTTGCATCgggc > 2:627660/1‑289 (MQ=255) aaTAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTGATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacg > 1:967198‑M2/1‑169 (MQ=255) aTAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGaa < 1:186251/290‑1 (MQ=255) aaaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCGGTCGTCCTTTCTACAAGCCATCCCCCCACAGATAAGGTAAACTCGCCTCGTTTTTGCATCTGGAAagc > 1:308891/1‑290 (MQ=255) aaaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcctcaaatcaaacgaacagctcagccgacagactgggccgttcggtttctctggtgtttgtcggtgacggctctcctgagtaggacaagcacgccgggagcgtgcttgcacgtttc < 2:611913‑M2/290‑126 (MQ=255) aaGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtcggacaaatccgccgggagcggatttgaacgttgcgaagca < 1:185676‑M2/290‑132 (MQ=255) aGGCTCAGTGGAAAGACTGGGCCTTTCGTTTTATCTTTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaa > 1:888965‑M2/1‑158 (MQ=255) gcacAGTGGAAAGACTGGGCTTTGGGTTTAATAGGTGGTGTGTGGGTGAACGC‑GTCCCGAGGGAGGACAAGTCCGCGGGGAGGGGATTGGAAGGTGGGGAAGCAACGGCCGGGGGGGGGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGaa < 2:202996/287‑1 (MQ=21) gCTCAGTCGAAAGACTGTGCCTTTCGTGTTATCGGTTGTTTGTCGGGTAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGaa < 1:578924/290‑1 (MQ=255) gCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggccaaatccgccgggagcggatttgaacgttgcgaagcaacg < 1:910719‑M2/290‑135 (MQ=255) gCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacg < 1:212467‑M2/290‑135 (MQ=255) cTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAAc < 1:284053/290‑1 (MQ=255) cTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaaagaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacgg < 1:499698‑M2/290‑136 (MQ=255) tCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAAcc < 1:218779/290‑1 (MQ=255) aagaccTGGGCCTTTCTTTTTATCGTTTGTTTGTCGCGGAACGCTCCCCGGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCtt < 2:502427/285‑1 (MQ=255) aaaTACTGGGCCTTTCGTGTTATCGGTTGTTTGTCGTTGAACGCTCTCCTGA‑GTAGGACATATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagg > 1:6548‑M2/1‑147 (MQ=255) aaaGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAAATACGGTAAACTAGCCTCGTTTTTTCATCAGGAAAGCCACCATGAACCAATCCtt > 2:102026/1‑290 (MQ=255) aCTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTCCAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATTAGGAAAGCAGCTATGAACCACTCCTTaaac > 1:357813/1‑289 (MQ=255) cTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAAc > 1:70630/1‑290 (MQ=255) gCCTTTCTTTTTAGCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAACCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggc > 1:414349‑M2/1‑138 (MQ=255) gCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaagctcagtcgaaacaccgggcctttcggtttaacctgtgtttgtcggtgaccactctcctgattaagcaaaatccaccgggagcggaattcaacgttccgacgcaacgggccggagggtgacagga < 2:447908‑M2/290‑153 (MQ=255) gTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAAcaca > 1:863432/1‑290 (MQ=255) gTTTTATCGGTTGTTTGGCTGTGAACGCCCTCCAGA‑GAAGGACAAATCCGCGGGGGGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGAAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgc > 1:706164‑M2/1‑131 (MQ=255) aTCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcagggcgcccgcc < 1:531233‑M2/290‑165 (MQ=255) aTCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcacgcc < 1:400569‑M2/290‑165 (MQ=255) cctGTTGTTTGTCGTTGAACTCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgcca > 1:224941‑M2/2‑125 (MQ=255) gttgttTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTAATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGACAACATTTGGCAtt > 1:962559/1‑290 (MQ=255) gttgttTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcccgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaaaggcgcggagggtggcgggcaggacgcccgccagaa < 1:309280‑M2/290‑169 (MQ=255) gtttgtCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACCAGCCATCCCCCCACAGATACGGTAAACTAGCCCCGTTTTTGACTACGGAAAGCAGCTATGAACCACTCCTTGAAACCCTGGAACCCATTTTGCATTgta > 2:465743/1‑288 (MQ=21) ttgtCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTACCCTCGTTTTTGCATCAGGACAGCAGCTCTGAACCCCACCTTCAAACCCTGGAACACAattagatttgcaca > 2:966198/1‑279 (MQ=21) ggTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCCCCGATACGGTAAACTAGCCTCGTTTTTTCACCAGGAAAGCCGCTATAGACACCTCCTTAAACACCTGGAACCACTTTGGCATTTATCCTAAtt > 2:690520/1‑289 (MQ=21) gAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgccataaactgcccggcatt < 1:595915‑M2/290‑182 (MQ=255) aaCGCGTTCCTGA‑GAAGGAAAAATCCGCCGGGGGCGGATTTGAACGTGGGGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgccataaactgccaggaatta > 1:233901‑M2/1‑108 (MQ=255) aCGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAg > 1:275820/1‑290 (MQ=255) aCGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTAGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCATGGAACACATTTGGCATTGATCATAATGCTCAg > 1:767477/1‑290 (MQ=255) tctctcCTGA‑GTAGGACAAATCCGCGGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAACCTGCCAGGAATTAAttccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgccataaactgccaggaattgggg > 1:192500‑M2/2‑105 (MQ=255) ctctCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCCAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATACACAAGCCATACCCCCACAGATACGGTAAACTAGCCCCGTTTTTGCATCAGGAAAGCAGCTATGAAACACTCCATAACAACCTGGAACCCATTTAGAACTGATCAACATGCTCAacac > 2:1041740/1‑290 (MQ=21) ctctCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATAATAATGCTCAGcac > 1:453457/1‑290 (MQ=255) ctctCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAACCCATCCCCCCACAGATCCGGTAAACTAGCCTCGTTTTTGCATCCGGAAAGCAGCTCTGAACCACCCCTTAAAACCCTGGAACCCCTTTGGCATTTATCAGaaatactacgca > 2:16435/1‑280 (MQ=21) cTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATAAACCACTCCTTAAAACCCTGGAACACATTTGGCATTAATCATACTGCTAAGCACA‑tttt > 2:854519/1‑288 (MQ=255) gTAGGAAAAATCCGCCGGGAGCGGATTGGAACGTTGCGAAGCAACGGCACGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTAtgt < 1:296795/290‑1 (MQ=255) aaaTCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAg > 1:741881/1‑290 (MQ=255) agcggcgggggggggATTTGAACGTGGGGAGGAAAGGCCCCGGGGGGGTGGGGGCAGG‑AGCCCGGCCATAAACGGCCAGGCATCAAAGTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCGTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGAc < 2:961968/277‑1 (MQ=38) ccgccgGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAagaaga > 1:316182/1‑290 (MQ=255) gggAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTCGCCTCGTTTTTGCATCATGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACACTTTGCATTCATCATAATGCTCCACACA‑CTGTATTTTCCgacgaccgaccac > 2:489222/1‑283 (MQ=25) gggAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTTCATCAGGAAAGCAGCTATGAACCACTCCTTAAAAACCTGGAACACATTTGGCATTGATCATCATGCTCAGCACA‑TTGTATTTGCCGAATATGaacaac > 1:156094/1‑290 (MQ=255) ggAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCAAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTAATATCTACAAGCCATCCACCCACAGATACGGTAAAATAGCCTCGTTTTTACATCAGGACAGCAGGAATGAAACACTCATTAAACACCTGGAACACATTTGGCATTGATCATATTGCACAG‑ACACTTTTATATGCCGagatccaacaaaa > 2:984187/1‑278 (MQ=21) aaaCGTGGCAAAGAAAGGGCGCGGGGGGTGGGGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATg < 2:407950/289‑1 (MQ=255) aaCGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCTGAAGACGAACAACAATTACTCAATGc < 1:136070/290‑1 (MQ=255) aCGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACC‑TTGTATGTGCCGAAGACGACCAACAATTACTCAATGcc > 1:1027872/1‑290 (MQ=255) gTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTCTGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTg > 1:256380/1‑290 (MQ=255) gTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTg < 1:293125/290‑1 (MQ=255) gTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCACCACA‑TTGTATGTGCCAAAGACTAACAAAAATTACTCAACACCTg > 2:707839/1‑290 (MQ=255) ttGCGAACAAACGGCCCGGAGGGTGGCGGGCAGG‑AGGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTgg < 1:588612/290‑1 (MQ=255) gcaaaGCAACGGCCCGGGGGGTGGCGGGCAGG‑CCGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCa < 2:70630/287‑1 (MQ=255) aaGCAACGGCCGGGGGGGGGGCTGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTa < 2:1004790/290‑1 (MQ=255) aGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTAt < 1:68986/290‑1 (MQ=255) ggCCCGGAGGGTGGCGGGCAGG‑CCGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAAcc < 1:114933/290‑1 (MQ=255) ggCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAAcc < 1:456962/290‑1 (MQ=255) ggCCCGGAGGGTGGCGGGCAGG‑ACGCCCACCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAAcc < 1:359165/290‑1 (MQ=255) gCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTACAACCCTGGAACCCATTTGGCATTGATCCTAAAGCTCAGCACA‑TTGTATGTGCCGAAGACCACAAACAATTAATAAATGACTGGGAGTATCGAACCGACGTAGGACAAc > 2:251997/1‑290 (MQ=38) aCGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTa < 1:477886/290‑1 (MQ=255) gcccgccATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTAtt > 1:459323/1‑290 (MQ=255) cccgccATAATAGGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCATCCGTTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTc < 2:375886/290‑1 (MQ=255) ccATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTggg < 1:275390/290‑1 (MQ=255) aaaCTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTgaa > 1:255772/1‑290 (MQ=255) aaaCTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGACGAAGACGAACACCAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTgaa > 1:954136/1‑290 (MQ=255) aCTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGCAGACGAACACCAATTACTCAACGCCTGGCAGTCTGCAACCGCAGAAGGACAACCCGTTCTTATTCATGGTgagcg > 2:21239/1‑287 (MQ=255) aCTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTgaagg > 1:558117/1‑290 (MQ=255) gCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTgaaggaag < 1:465825/290‑1 (MQ=255) cAGGCATCAAAGTAAACAGAAGGCCAGCCTGCCGGATGGCCTTGTTGCGGTTCTACAAACTCGTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTa < 2:303286/290‑1 (MQ=255) gCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACCATTACTCAATGCCTGGCAGTAAGCAACCGCCGCATGACAACCCGTTCTTATTCTTGGTGAAGGAAGTAAtt > 2:963358/1‑289 (MQ=255) | TTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT‑GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA‑GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑ACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACA‑TTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATG > NZ_LN832404/4168108‑4168683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |