Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	4,303,525	T→C	75.3%	N250D (AAT→GAT)	alsK ←	allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	4,303,525	0	T	C	75.3%	145.5 / 52.2	81	N250D (AAT→GAT)	alsK	allose kinase
Reads supporting (aligned to +/- strand):  ref base T (8/12);  new base C (26/35);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

AACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACC  >  NZ_LN832404/4303238‑4303807                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                           aaCTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCCGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATcca                                                                                                                                                                                                                                                                                          >  2:377847/1‑290 (MQ=255)  acgtgcATTGCAGAAAATAAGATGATTCTTTTTAGGTGTTTATTATATAGATGGTACCGGTCGTTGGCACAGCGGTACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCATTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATccat                                                                                                                                                                                                                                                                                         <  1:755569/285‑1 (MQ=255)    tagtaTTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATAGTGTACCGGTGGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATccatca                                                                                                                                                                                                                                                                                       <  1:387744/286‑1 (MQ=255)     aTCATTGCATAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCAc                                                                                                                                                                                                                                                                                      <  1:871694/290‑1 (MQ=255)           taaaaaaaTAAGATGAGTCGTTTTATTGTTTTATTATATTGATTGTACCGGTCGTTGGCACAGCGGGACATTGCGCTACTGGGCCAACG‑TGTTACTAATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCAcc                                                                                                                                                                                                                                                                                <  2:1008831/286‑1 (MQ=255)           gCAGAAAATAATATGAGTCGTGTTAGGTGTGTATTATATATATGGTACCTTGCGTTGGCAAAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑GGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCAcc                                                                                                                                                                                                                                                                                <  1:851393/290‑1 (MQ=255)            cAGATAATAATATGATTGTTTTTAGTTGTTTTTTATAGATATTGTACCTGTCGTTGGACCAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACATAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGTCATATCCATCACGCCACCg                                                                                                                                                                                                                                                                               <  2:60928/290‑1 (MQ=255)             aGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGc                                                                                                                                                                                                                                                                              >  1:61747/1‑290 (MQ=255)             aGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGACACCGc                                                                                                                                                                                                                                                                              >  1:963069/1‑290 (MQ=255)              gAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGcc                                                                                                                                                                                                                                                                             >  1:1047865/1‑290 (MQ=255)               aaaaTAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGccc                                                                                                                                                                                                                                                                            <  1:827905/290‑1 (MQ=255)                aaaTAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCAAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTACAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCCAGCGTGGGAAGGCGGGCCAAGCCATAACGCCACCGCCCa                                                                                                                                                                                                                                                                           >  2:940169/1‑290 (MQ=255)                     atatGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATc                                                                                                                                                                                                                                                                      >  1:1066191/1‑290 (MQ=255)                             cGTGTTATGGGATTATTATATATATTGTACCTGTCGGTGGCACGGGTGGAACGTGCGGGACTGTGCCAACG‑TTTTACTCATGGGGCTTGAGCACAGAACTG‑GGGCAAAAAACGTGGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATc                                                                                                                                                                                                                                                              <  2:990759/290‑1 (MQ=255)                              tttttttttGTTTATTATATATCTTGTACTGTTCGTTTGTACCGCGGTCCTTTGCGCTCCGTTGCTCACT‑TTTTTCTCATGGGGCTGTAGCACATAACGT‑TGGCAAAAAACGTTGCGTCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAATATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCg                                                                                                                                                                                                                                                             <  2:729165/289‑1 (MQ=255)                                                    atTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGACGGAGGGCATATCCATCACGCCACCGCCAAGGATCACCGCATCGGGATCGAACCGATTAATGAtgg                                                                                                                                                                                                                                       >  1:942725/1‑290 (MQ=255)                                                    atTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGCACAGATTAATGCtgg                                                                                                                                                                                                                                       >  1:839516/1‑290 (MQ=255)                                                       gTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATTGGGGTCGGACAGCTTAAAGCtggggg                                                                                                                                                                                                                                    >  2:229051/1‑290 (MQ=255)                                                         aaccgTCGTGGGAACAGCGGGACATTGCGCTACTGTGCCAAGG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCa                                                                                                                                                                                                                                  <  2:297929/286‑1 (MQ=255)                                                             gTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATgg                                                                                                                                                                                                                              >  1:152842/1‑290 (MQ=255)                                                             gTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAAGGCTGGTCGCACTgg                                                                                                                                                                                                                              >  1:22575/1‑290 (MQ=255)                                                               cGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCGAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGcc                                                                                                                                                                                                                            >  1:304202/1‑290 (MQ=255)                                                                gTTGGCAGCGCGGGACATTGCGCTACTGTCCCATCG‑TTGTACTCAGGGTGCTTTATCCCAG‑ACTGTTGGCAAAAACCTTTGATGCCCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGccc                                                                                                                                                                                                                           <  2:113845/290‑1 (MQ=255)                                                                gTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGccc                                                                                                                                                                                                                           >  2:90646/1‑290 (MQ=255)                                                                    gCACAGCGGGACATGGCGCTACTGGTACAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑GGGCAAAAAACGTTGATGCGCCAATATTGCGGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGc                                                                                                                                                                                                                       <  2:279108/290‑1 (MQ=255)                                                                              aCATTGCGCTACTTTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCa                                                                                                                                                                                                             <  1:312010/290‑1 (MQ=255)                                                                              aCATTGCGCTACTGTGCCAACG‑TGTGAGGAAGGGGTCTGTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGttttaa                                                                                                                                                                                                             >  1:894111/1‑288 (MQ=255)                                                                               cATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCCACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAAAAGATTCATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCaa                                                                                                                                                                                                            >  1:672828/1‑290 (MQ=255)                                                                                agtgggcTACTGTGCGAACT‑TTTTACTCATGGGGCTGTAGCACAGAACTG‑TGGCAAAAAACGTGGATGCGCCAATATTGCTGCACCCTGAGCGCCAGTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAg                                                                                                                                                                                                           <  1:463635/285‑1 (MQ=255)                                                                                 agtgcggaatggtgcAACGTTTTTACGCATGGGGCTTTAGCACAAAAAGG‑TGGCAAAAAAAGTGTATGAGCCAATATGGCTGCACCCTGAGCGCCAATAAAGTCAGAAGAGGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAg                                                                                                                                                                                                           <  2:119486/276‑1 (MQ=255)                                                                                     gcTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGAAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGCTTAATGCTGGTGGCAATGGCCCGTGCCGAGATTTCAAGAAGAc                                                                                                                                                                                                      >  1:256052/1‑290 (MQ=255)                                                                                            tggtaaCG‑TTTTACTCAAGGGGCTTGAGAACAGAACTG‑GGGAAAAAAACTGTGATGGGCCCATATTGCTGCACCCTGCGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGTGCAGGTACTTTTGGGTCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGAc                                                                                                                                                                                               <  2:212138/286‑1 (MQ=255)                                                                                             gCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACg                                                                                                                                                                                              >  1:855893/1‑290 (MQ=255)                                                                                                  cG‑TTTTACTCAGGGGGTGTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAgg                                                                                                                                                                                         <  1:632332/290‑1 (MQ=255)                                                                                                   g‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAggg                                                                                                                                                                                        <  1:229051/290‑1 (MQ=255)                                                                                                     ttgtACTCGTGTGTCTTTGTCAAAGAACTG‑GGGTAAAAAACTTGGATGCGCCAATATTGTTGCACCCTGAGGGGCAGTAAAGTCAGAAGATGGGGCGGCAATAAAGCGAACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAgggg                                                                                                                                                                                       <  2:731316/287‑1 (MQ=255)                                                                                                      tttACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGc                                                                                                                                                                                      <  2:800078/290‑1 (MQ=255)                                                                                                         aCTCAGGGGGCGTTAGCACATAACTG‑TGGCAAAAAACGTTGATGCGCCAATATGGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGtt                                                                                                                                                                                   <  1:432416/290‑1 (MQ=255)                                                                                                                        gaaaaGAACGG‑TGGCAAAAAACGTGGAGCCGCCAAAATGGCTGCACCCTGAGCGCCATTAAAGGCAGAAGATGAGGCGGCAATAAAGCGAACGACCTGATGCGGCAGTGGACGGCGCAGGTACTGTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGaa                                                                                                                                                                    <  2:315311/286‑1 (MQ=255)                                                                                                                         cacaGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGCTCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTAAAGCAGACTCTGGACGAAAGGGGCGTTTTCCACATGGACGaaa                                                                                                                                                                   >  1:1047083/1‑290 (MQ=255)                                                                                                                           caGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGCAGGCGGGCATAACAATCACGCAACCGCCCCGGCTAACCGCCTCGGGATCACACAGCTTCATGATGGTTGCAATGGACCGTGCCCGGTATTCAAGCCAACACTGGCCAGCAGGGGCGTTTTACGCATGGAAGATAAg                                                                                                                                                                 >  2:811430/1‑290 (MQ=255)                                                                                                                             gAAGTG‑TGGCAAAAAACGTTGATGCGCCAATATTGGTGCACCCTGTGCGCCATTAAAGTCGGAAGATGGGGCGGCAAGAAAGCGCACGACCGGAGGCGGCAGGGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGAt                                                                                                                                                               <  2:616957/290‑1 (MQ=255)                                                                                                                                cTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGACGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGACAGGGGCGTTTTCCGCATGGACGAACACATcgc                                                                                                                                                            >  2:664952/1‑290 (MQ=255)                                                                                                                                 tg‑tgGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATcgcg                                                                                                                                                           >  1:454282/1‑290 (MQ=255)                                                                                                                                  g‑tgGCAAAAAACGTTGATTCGCCAATAGTGCTGCAACCTGAGCGCCAGTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCgcgc                                                                                                                                                          <  1:543993/290‑1 (MQ=255)                                                                                                                                   ttgGCAAAAAACGGTGATGTGCCAATATTGGTGCACCCGGAGCGCCAGTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCgcgc                                                                                                                                                          <  2:808619/289‑1 (MQ=255)                                                                                                                                    tgGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACCCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCa                                                                                                                                                         <  1:90646/290‑1 (MQ=255)                                                                                                                                      gCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGGGGGAAGGCGGGCATATTCATAACGCCACCGCCCAAGCTCACCGACTCGGGAGCGAACAGCTTCATGCTAGTCGCAATGGCCCTGGCCGCGTTTTCATGCATACTCAGGACGCACACGCCGTTTTCCTCGTGTACTAAACGacagagcacc                                                                                                                                                       >  2:908256/1‑281 (MQ=255)                                                                                                                                               gTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAAttt                                                                                                                                              >  1:236133/1‑290 (MQ=255)                                                                                                                                                   aggcgcAAATATGGGAGCACCCGGGGCGCCAAGAAAGGAAGAAGATGAGGCGGCAATAAAGCGAACGACCTGATGCGGCAGTGGAGGGCGCGGGTACTTGTGGGTAATGGCAACGAGAGTCTCGCGTGGGAAGGAGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCggg                                                                                                                                          <  2:959932/288‑1 (MQ=255)                                                                                                                                                   aTGCGCCAAGATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCggg                                                                                                                                          <  1:646467/290‑1 (MQ=255)                                                                                                                                                              ttGCAGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCg                                                                                                                               <  1:116170/290‑1 (MQ=255)                                                                                                                                                                           aGCGGCATTAAAGGCAGAAGAGGAGGCGGCAATAAAGCGCAGGACCGGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGGCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCa                                                                                                                  <  2:855893/290‑1 (MQ=255)                                                                                                                                                                              gcCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTTGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAgcg                                                                                                               >  1:173229/1‑290 (MQ=255)                                                                                                                                                                              gcCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAgcg                                                                                                               <  1:57207/290‑1 (MQ=255)                                                                                                                                                                                 aTTAAAGTCAGAAGAGGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCa                                                                                                            <  1:1034152/290‑1 (MQ=255)                                                                                                                                                                                         cAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGCACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCcgag                                                                                                    >  1:250891/1‑290 (MQ=255)                                                                                                                                                                                             agagGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAt                                                                                                <  1:65058/290‑1 (MQ=255)                                                                                                                                                                                             agaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAt                                                                                                >  1:687822/1‑290 (MQ=255)                                                                                                                                                                                              gaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCCTGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAtt                                                                                               >  1:494626/1‑290 (MQ=255)                                                                                                                                                                                                ggagagggaaaTAAAGAGCAGGCCCGGCTGGGGCATGGGACGTGGAAGGTACTTTTGGGTCATGGCAAGGAGAGTCGCGCGTGGGAAGGCGGGCAAATCCATACCGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTgg                                                                                             <  2:810581/281‑1 (MQ=255)                                                                                                                                                                                                  gggcggcAATAAAGCGCACGACCGGATGCGGCAGGGGAGGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGtt                                                                                           <  2:149342/289‑1 (MQ=255)                                                                                                                                                                                                         aaTAAAGAGCACGAGTGGATGCGGGAGGGGACGGTGCAGTGTGTATTGGGTGTGGGCAACGAGAGCCTGGCGTGGGAAGGCGTGAATATGCAGCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAAGGCTGGTGGCAATGGTCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGc                                                                                    <  2:695004/290‑1 (MQ=255)                                                                                                                                                                                                            aaaGCGCCCGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGTGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCAcc                                                                                 <  1:416748/290‑1 (MQ=255)                                                                                                                                                                                                             aggcgaaggACTTGATGCGGCAGTGGACGGCGAAGGTACTTTTGGGTCATGGCAACGAGGGGTGCGCGGGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCAccc                                                                                <  2:914281/282‑1 (MQ=255)                                                                                                                                                                                                                cgcACGACCGGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAgg                                                                             <  1:975868/290‑1 (MQ=255)                                                                                                                                                                                                                   aaggccTGATGGGGCAGGGGACGGCGCAGGTACGTTTGGGTCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGAAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGAtt                                                                          <  2:570109/286‑1 (MQ=255)                                                                                                                                                                                                                   aCGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCACGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGACAAGATCGCGCACTGGCTAATTTCGGGGCTGTTTTTCGGACCAGCGTCTTAGCCCCCTTCCAGCGCCATTGGTTTCGAGGCAccgcgcacg                                                                          >  2:82688/1‑283 (MQ=255)                                                                                                                                                                                                                     gACCTGATGCGGCAGTGGACGGCGCAGGTACTTGTGGGGCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGc                                                                        <  1:600979/290‑1 (MQ=255)                                                                                                                                                                                                                      acctgtgggggCAGGGGACGGAGCAGGGACTTTTGGGTCAGGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTGTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGcc                                                                       <  2:687822/281‑1 (MQ=255)                                                                                                                                                                                                                        cTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGcccc                                                                     >  1:953859/1‑288 (MQ=255)                                                                                                                                                                                                                          gtggcgtgggTGGATGGGGCTGGTGCTTTGGGGTCCTGGCAACGCGAGGCTCGCGGTGGAAGTCTGTCATAGCCAGTACGCCACCTCCCAGGATCACCGCATCGGGAGCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCacac                                                                   <  2:616007/281‑1 (MQ=255)                                                                                                                                                                                                                           aTGCGCCAGTGGCCGGCGCAGGTACTTTTGGGTCATTGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCGCCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAcg                                                                  <  1:732801/290‑1 (MQ=255)                                                                                                                                                                                                                               ggCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCgcgcc                                                              >  1:971723/1‑289 (MQ=255)                                                                                                                                                                                                                               ggCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCa                                                              <  1:283194/290‑1 (MQ=255)                                                                                                                                                                                                                                 cAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCGGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAgt                                                            <  1:325138/290‑1 (MQ=255)                                                                                                                                                                                                                                 cAGTGGACGGCGAAGGTACGTGTGGGTCATGGCAACTGTGGCCTCGCGGGGGAAGGCGGGCCTATCCATCACGCCACCGCCCAGGATCACCGCCTCGGGGTCGAACAGAGTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAgt                                                            <  2:464642/290‑1 (MQ=255)                                                                                                                                                                                                                                        cGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGt                                                     <  1:377847/290‑1 (MQ=255)                                                                                                                                                                                                                                         cgcggaaggacgtatGGGGCAAGGCAAGTAGAGGCTCGAGTGGGAAGGCAGGAAGATCCAGCAAGCCAACGCCCAGGATCACCGCATCGGGATCGAACAGAATAATTGTGGTGGAAAGGGCCCGTGCCGCGTGTGCAAGCAGACGCTGGACGAAAGGGGCGGTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTGCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTc                                                    <  2:293982/276‑1 (MQ=255)                                                                                                                                                                                                                                           cgcAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGCTTGCCACACGCGCAGTGTTGGGTCat                                                  >  1:967219/1‑290 (MQ=255)                                                                                                                                                                                                                                             cAGGTACTGTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCCAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCatat                                                <  1:465356/290‑1 (MQ=255)                                                                                                                                                                                                                                              aGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGACAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCACTTGGTTTCCAGCCACCAAGGATGACCAACCGGCCATTGTTGGGTCatacc                                               >  1:1064135/1‑288 (MQ=255)                                                                                                                                                                                                                                                  tctcttggtgcATGGTAACGGGAGCCTCTCGTGGGACGGCGGGCATCGTCACCACGCCACCTCCCAGGATCACCGCATCGGGATCGAACAGATGAATGCTGGTGGCAATGGCCCGGGCCGCGTTTTCAAGCAGACGCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTccc                                           <  2:870954/280‑1 (MQ=255)                                                                                                                                                                                                                                                          gTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                                   >  1:663149/1‑290 (MQ=255)                                                                                                                                                                                                                                                            cATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGatat                                 <  1:543172/290‑1 (MQ=255)                                                                                                                                                                                                                                                                  aaCGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCCTTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAAGCGCAGTGTTGGGTCATATCGCCCAGGGGGATATGACGCa                           >  1:270562/1‑290 (MQ=255)                                                                                                                                                                                                                                                                      agagTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATAACCCAGTTc                       >  1:806654/1‑290 (MQ=255)                                                                                                                                                                                                                                                                      agagTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTc                       <  1:187434/290‑1 (MQ=255)                                                                                                                                                                                                                                                                           ctcGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCACTGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCAATTCCACAGCAATTGGTTTCCAGGCCCCACGGCTTGCCCCACGCCCCGTGTGCAGTCCAATCCACACGGGGGATATGACCCCGtcagccag                  >  2:889847/1‑283 (MQ=255)                                                                                                                                                                                                                                                                                   ggAAGGCGGGCATCTCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCg          <  1:222647/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aaGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGCGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCCGGGTTGGGTCATATCCCCCAGGGGGCTTGGACCACGTTCGCCTGCCCCACCgtg        >  2:386077/1‑290 (MQ=255)                                                                                                                                                                                                                                                                                      aGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCCCACCgttt       >  1:91685/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                      aGGCGGGCAGATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgtgt       <  1:831510/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                           tgCAGATCCATAACGCCACCTCCCAGGATATCCGCTGCGGGATCGAACGAGTTAAGGCGGGTGGCAATGGCCGGGGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGTGCGGTGTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGCTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAGTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAcc  <  2:837607/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                           AACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACG‑TTTTACTCATGGGGCTTTAGCACAGAACTG‑TGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACC  >  NZ_LN832404/4303238‑4303807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑