| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 4,023,947 | G→A | 40.8% | intergenic (+246/+135) | EK12AG100_RS19960 → / ← fadA | NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 4,023,947 | 0 | G | A | 40.8% | 20.8 / 21.9 | 22 | intergenic (+246/+135) | EK12AG100_RS19960/fadA | NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA |
| Reads supporting (aligned to +/- strand): ref base G (10/3); new base A (4/5); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
CCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGATGACCCAGCGCGATCGCGCCA > NZ_LN832404/4023671‑4024238 | ccTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcg > 1:359295/1‑296 (MQ=18)ccTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcg > 1:725445/1‑296 (MQ=18) tGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgcc > 1:360762/1‑296 (MQ=18) tttGGCGATGCTATCGCATTTAGCTGGGATAGAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGGTATTCAGTGGCATTTAGATCTATGACGTATCTGGCGAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgccgc < 2:449977/296‑1 (MQ=17) gCGATGCGTTTGCATTTAGCTTAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACGAAACTGGTATTCAGTGGCAGTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCGGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑AGT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATcc < 1:479416/296‑1 (MQ=17) gttgtcATTTATCTGGGATAACAAAAACCCCGCCCCTGACGTGCGGGAAGAACGGCAACTAACCTGTGATTCAGTGGCATTTAGATCTATGACGTACCTGGCAAACCATGCCCGATGCGACGCGGTCGCGTCTGATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGAGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCaagt < 2:180999/291‑3 (MQ=14) ttGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTgg > 1:796293/1‑296 (MQ=17) gtGCATTTAACTGGGATATAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTgg < 1:111429/295‑1 (MQ=17) tATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGGGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATT‑GGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaacc < 1:208737/296‑7 (MQ=14) tCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCAATTGGTGCATGATGCCTGATGCGACGCCGGCGCGTCTTCTCAGGCCTAC‑ATT‑GGTACCGGCTCGGTAGACCGGCAAAGGCGTTCCCGCCCCATCCGGCaagcggttaaacccg > 2:293229/1‑283 (MQ=17) cTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTTGTGC‑CG‑GCTCGGTAGACCGGATCAGGCGGTTCCGCCGCATCCGGCCAGTGGTTCaccccgc > 2:35177/1‑290 (MQ=17) gtatcagtcaaaccatgCCCGATGCGACGCTGTCGCGTCTGTTCGGGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGa < 2:417838/282‑1 (MQ=255) ctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCCCAACACCCTCACCCCGACCGATACACATCCTCCCCCGACCCACCTGACCGGCTTTGCCTTCCCCCACATTcc > 2:351348/11‑295 (MQ=18) aaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCagcagc > 1:433954/6‑296 (MQ=255) tgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGCACGTCTTTGCGTTCCATCAGATTCAGCAGCgtggtg > 1:754063/1‑296 (MQ=255) tgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCgtggtg > 1:99895/1‑296 (MQ=255) tgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCCCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCCAACCGAACGTCTTTGCCTTCAATCAGCTTCAGCAGCgcggtg > 2:526412/1‑296 (MQ=255) cgATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGCCCGACACCCATCGTCCCCCGACCAAACCGACCGTCCTTGCCTTCCATCAGCTTCAGCACAGTGGTGCtcc > 2:820045/2‑294 (MQ=25) cgATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGa < 1:654197/295‑1 (MQ=255) aCGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACg‑cgc > 1:54881/1‑296 (MQ=255) cgtcgttgcgggCCTACAAAC‑AGC‑CC‑GAACCGTAGGCAGGGTAAGGCGTTTACGCCTCATCCGGCAATTTGTGCAGGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGaaca < 2:449904/285‑1 (MQ=255) tCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTCAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGCTACACATCGTCGCCAGCCCAAACTGAACGCCTTTGCGTTACACCAGCTCCAGCACCGTGGCGCGGATCCG‑CGCACCgtccccac > 2:771717/1‑289 (MQ=25) ggccgacgatgaggcc‑GAACCGTGGGCCGGTTAAGGCGTTTACGCCGCAGCCGGCAATTGGGGCAGGATGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGa < 2:725445/282‑1 (MQ=255) aCAAAG‑AGT‑CC‑GGACCGTGGGCCGGATACGGGGTTTACGCCGCATCCGGAAATTGGGGCATGAGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGATGAcc < 2:360762/296‑1 (MQ=255) t‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGATGACCCAGCGCGa < 1:841998/296‑1 (MQ=255) gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGATGACCCAg < 2:390889/288‑1 (MQ=255) gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGATGACCCAg > 1:390889/1‑288 (MQ=255) cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGG‑GCCGAATCGCCCCCCCGGACCAACCAAGCGGATGACCCacccggaccgccca > 1:254561/1‑283 (MQ=37) ggaGGGCGGAGAATGGGGTTACGCCGCACCCGTGAATTGGTGCATGTTGCCTGATGCGACGTGGGGGCGTCTTAGCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACGTCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGATGACCCAGCGCGATCGCGCCa < 2:774318/294‑1 (MQ=255) | CCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAT‑AGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACG‑CGCACCGGAACAACCCAGCGGATGACCCAGCGCGATCGCGCCA > NZ_LN832404/4023671‑4024238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |