BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	97.6%	424.1 / ‑0.5	125	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (58/64); total (61/64)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 127 total aligned reads displayed.

TCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560206‑3560698

tcttctTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAAACGAACTGGAAGGCAGCGCCGCCCCGGCATGAT‑GG‑cg                                                                                                                                                                                                                                                      >  1:1123762/1‑250 (MQ=255)

   gcttTTACCATCAACGTGTTAGCAACGTTTAGTTTGTTGGTGACAATGCGCAAATTGCTGTGATTGTGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cgcag                                                                                                                                                                                                                                                   <  2:1486134/250‑5 (MQ=255)

       gtataagagacagtttAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATATACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCAATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGAGTGTTAACCGAACTGAAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATc                                                                                                                                                                                                                                               <  2:1501787/237‑1 (MQ=255)

                aCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCATCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                      >  1:333937/1‑251 (MQ=255)

                     ttAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGc                                                                                                                                                                                                                                 <  2:829582/251‑1 (MQ=255)

                       aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCa                                                                                                                                                                                                                               <  2:1123517/251‑1 (MQ=255)

                               tgtgatttatgGTGACAATGCGCAAATTGCTGTGATTGAGATGCGCGTGCGCTACCGCTTGCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                       <  2:1492022/242‑1 (MQ=255)

                                aggaTTTTTGGGGACAATGCGCAAATTGCTGTGATGGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATACACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTAGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                                                                                                                                                                                      <  2:683717/249‑1 (MQ=255)

                                      gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                >  2:422064/1‑251 (MQ=255)

                                          gTGACAATGCGCAAATTGCTTTTATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                            <  1:1325794/251‑1 (MQ=255)

                                           tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACTGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTTGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                                                                                                                                           >  2:1246151/1‑251 (MQ=255)

                                           tGACAATGCGCAAATTGCTGTGATTGAGCAGCGCGTGCGCTACCGCTTCCGGCGTGGTCCCGATATCGATAAACAGCGTCGAGCCAATGGGGATTTGCTCCGCGACCTTGCGGGCGATGCGCGATTTTTATTGGTTCTGGGTGGCCGTGAGATCGTGCCACGGCGAGTTAACCGAACTGGACGGCAGCGGCGCAGCGCTATTAT‑GGAC‑CAGGCTCAGGCTTTGCTCCGCCAGCTCATTCAgtctgaggcgt                                                                                                                                                                                                           >  1:1094962/1‑241 (MQ=255)

                                            gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                                                                                                                                          >  1:77562/1‑251 (MQ=255)

                                                aggaggcaaTTGCTGGGTTTGTGCAGTGCGGGCGCTACCGGTCCCGGCGTGGTGCCGATAACGATAAACAGCGTCGGGCCATTCGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCGTGTTCTGCGGTCGGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                                                                                                                                      <  1:1482046/244‑1 (MQ=255)

                                                  gcgcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATc                                                                                                                                                                                                                                               <  2:502326/208‑1 (MQ=255)

                                                   cgcAAATTGCTGTGATTTAGCAGTGCGTGCGCTCCCCCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGTGCCATTCGGGTTTTGCCCCGCCACTTTTCGCGCGACCCCCCCTTCTTCTTAGGTCTGGGTGGCCTTGCGATCTTGCCACGCCGTGTTCACTGCACTGGAAGGCAGTGCCCCACTGACATGAC‑GG‑CGCATGATCCGGTTTTGCTCCGTCATCCCATTGACGTCCCGGCGAAtcatttg                                                                                                                                                                                                   >  1:950859/1‑245 (MQ=255)

                                                   cgcAAATTGCTGTGATTGCGCAGTGCGTGAGCTACCGCTTCCGGAGTGGTGCCGATATCGATAAAAAGCGTCGGGCAATTGGGGATTCGCTACGCCACTTTGCGGGCGCCGCGCCATTCTTCTTCGGTCTGCGTGGACTCGCGATCGTGCCAAGGCGTGATAACCGAACTGGAAGGCAGCGCCGCACCGCAACGAT‑GGTG‑CAGGTCAAGGTACTGCACCGCCACCGCATTggaggcgaggagaagaggacg                                                                                                                                                                                                   >  1:1537722/1‑231 (MQ=255)

                                                   cgcAAATTGCTGTGATTGAGCGGTGCGTGCGCTACCGCTTCCGGCGTGGGGCCGCTAGCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                                                                                                                                   <  1:367205/251‑1 (MQ=255)

                                                   cgcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGACGCGCTCTTTTTCATCGGTCTGGGTGGCCTTGCGATCGTGCCAAGGCGTGTTAACCGCACTGGAAGGCATCGCCGCAACGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTACTCCAGCTCACTTAGGTCGGGGCGAAGAGTCTg                                                                                                                                                                                                   >  2:1543281/1‑251 (MQ=255)

                                                      agaggcaggtgATTGAGCAGGGCGGGCGCTGCCGCTGCCGGCGGGGGGCCGATATCGGTAAGCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGGGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                                <  1:65792/243‑1 (MQ=255)

                                                        aTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                              <  2:1399302/251‑1 (MQ=255)

                                                          tGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCTACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTTTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGACGCATCGCCATGAT‑GG‑CGCAGTATACGGGTTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCCGCggggct                                                                                                                                                                                            >  1:683893/1‑248 (MQ=255)

                                                              gtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTCGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCGGCGGGCTGACgg                                                                                                                                                                                        >  1:526398/1‑251 (MQ=255)

                                                              gtgATTGAGCAGTGCGTGCGCTACCGCTTCCGCCGTGGTGCGGCTCTCGATAAATAGCGTCGAGCCATTGGGGATTTGCTCCACCAGTTTGAGGGCCCTGCCCTCCTTTTTCTCGCTTTGGGTGGCCTTTATATTTTGTTACGGTGTGTTATGCTCACTGGTAGGCATCGTCGCACTCACTTAATCGT‑C‑CTGCTTCCGTTTATGCTCCCCCACTTCATTGAGGACGCGGCGAAACTTCAGCGTGCTGAcca                                                                                                                                                                                        >  1:929519/1‑249 (MQ=255)

                                                                 aTTGGGCAGTGCATGCACTACCGCTTCCGGCGTGGTCCCGCTATCGATAAACGGCGTCTCGTCATTGGGGATTTGCTCCGCCAGTTTGCGGGCGCTGCGATCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGAAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                                                                                                                                     <  1:422184/251‑1 (MQ=255)

                                                                      gCAGTGCGTGCGCTACCACTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAGTTGGGATTTGCTCCGCCACTTTGCGTGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTTGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                                                                                                                <  1:724008/251‑1 (MQ=255)

                                                                         gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATc                                                                                                                                                                                                                                               <  2:1326273/185‑1 (MQ=255)

                                                                           gcgtgccCTACCGCTTCCGGTGTGCTGCCGATATCGTTAAACAGATTCGCGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCCCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGCATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                                                                                                                           <  2:1284901/251‑1 (MQ=255)

                                                                            cttgcgCTCCCGCTTCCGGCGTAGTGCCGATATCGATAAACAGCGTCGAGCCATTTGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCAAGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                          <  2:804985/249‑1 (MQ=255)

                                                                            cttgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCAATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCAATCGTGCGACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                          <  2:1397485/249‑1 (MQ=255)

                                                                                    aCCGCTTCCGGCGAGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCAGCCAGCTCATCGAGGTGGCGGCGAATAGTCTGAGGGCGTCCGGCGAAATgatagacaagcctttc                                                                                                                                                                  >  1:958338/1‑236 (MQ=255)

                                                                                      cGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGTCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCTCGGCGAATAGTCTGAGGGCTGACGGAGAAATGCTCTATCAGCTCTTAgg                                                                                                                                                                >  2:621498/1‑251 (MQ=255)

                                                                                      cGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                                                                                                                                      <  2:42169/213‑1 (MQ=255)

                                                                                      cGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                        >  2:65730/1‑243 (MQ=255)

                                                                                       gCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                               >  2:1484810/1‑251 (MQ=255)

                                                                                       gCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGt                                                                                                                                                               >  2:1420824/1‑251 (MQ=255)

                                                                                        catCCGGCGTGGTGCCGATATCGATAAGCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                              <  2:52652/249‑1 (MQ=255)

                                                                                        cTTCCGGCTTCGTGCCGATATCTATCAAAAGGGTCTAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑AG‑CGCAGGATCGGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                              <  2:1094722/251‑1 (MQ=255)

                                                                                        cTTCCGGCGTGGTGCCGATATCGATAAACGGCGGCGGCGCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTGTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                              <  1:140529/251‑1 (MQ=255)

                                                                                        cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                              <  1:1165045/219‑1 (MQ=255)

                                                                                        cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                              >  2:723827/1‑251 (MQ=255)

                                                                                        cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCAATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                              <  2:1310319/251‑1 (MQ=255)

                                                                                        cTTCCGGCGTGGGGCCGTCTCCGTTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCCGTTTGCGGGCGGTGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCTGTCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                              <  1:1084957/251‑1 (MQ=255)

                                                                                         ttCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAg                                                                                                                                                                                                       >  2:549760/1‑209 (MQ=255)

                                                                                         ttCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGACGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACTGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                             >  2:788121/1‑251 (MQ=255)

                                                                                         gtcgggcgtggagccttgttcgtgaaaCAGCTTGGGGCCATTGGGGGTTTGTTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGAGTGGCCTTGGGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGAACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                             <  1:1426211/227‑1 (MQ=255)

                                                                                            cGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTCCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTCGCGATCGTGCCACGGCGTGTTAACCGCACTGGACGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGAACAGGTTTTGCCCCGCCAGCTCATGGAGGGCGCGGCGAATAGTCCGCGGGCTGCCGGGGAAATGCCCTACCATCTCTTCTGTACtta                                                                                                                                                          >  1:1557232/1‑249 (MQ=255)

                                                                                                 tggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGAT‑GG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCACATTGAGGTCGCGGCGAATAGTCTGCGGCCTGACGGAGAAATGCTCTCCCCGCTCTTCGGTACTGGCA‑Tac                                                                                                                                                    >  1:504843/1‑250 (MQ=255)

                                                                                                 tggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCCCTTCGGTCCTGACC‑Taa                                                                                                                                                    >  1:1039317/1‑251 (MQ=255)

                                                                                                 tggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCCGCTCATTg                                                                                                                                                                                                                       >  1:1326532/1‑185 (MQ=255)

                                                                                                 tggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                         >  2:1385307/1‑199 (MQ=255)

                                                                                                 tggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                         >  2:549698/1‑199 (MQ=255)

                                                                                                 tggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                         >  2:873487/1‑199 (MQ=255)

                                                                                                     gCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCct                                                                                                                                                <  2:1039091/251‑1 (MQ=255)

                                                                                                     gCCGAGATCGATAAACAGCTTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGTTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCct                                                                                                                                                <  2:1381342/251‑1 (MQ=255)

                                                                                                     ccctagaacggtAAACAGCGTCGGACCATTTGGGAGTTGCTCCGCAACTTTGCGGGCGAAGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGAGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCct                                                                                                                                                <  2:481744/240‑1 (MQ=255)

                                                                                                     accGATAGCGATAAACAGCGGCGCGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCct                                                                                                                                                <  1:1485096/250‑1 (MQ=255)

                                                                                                     acacatagcggttaactgcttggAGCCATTGTGGTTTTGCTCCGCCTCTGTGCGTGCGATTCGCTCTTTTTCGTCGGTCTTGGTGCCCTTGCGCTCTTTCCACGGCGTTTTGACCTGACTGGAAGGCAGCGCCGCTCCGCCATGAT‑GG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCA‑TAACCct                                                                                                                                                <  1:288615/229‑1 (MQ=255)

                                                                                                          tatCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGCACTGACA‑TAACCCTGCTGt                                                                                                                                           >  1:1331599/1‑251 (MQ=255)

                                                                                                          tatCGATAAACAGCGTCGAGCCATTGGAGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGt                                                                                                                                           <  2:516487/251‑1 (MQ=255)

                                                                                                             cGATAAGCGGCGACGAGCCATTGGGGATTTGCGCCGCCACTTTGCGGGCGATGCGCTCTGTTTCTTCGGGCTTGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCACCATGAT‑GG‑CGAAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTa                                                                                                                                        <  1:770093/251‑1 (MQ=255)

                                                                                                                    cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGtt                                                                                                                                 <  2:715903/251‑1 (MQ=255)

                                                                                                                      gggTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGGGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCg                                                                                                                               <  2:708583/249‑1 (MQ=255)

                                                                                                                       cGTCAAGCCGTTGGGGAATTGCTCCGCCACTTTGCGAGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTGCTGACA‑TAACCCTGCTGTTTAACCAGTTCGa                                                                                                                              <  2:1242239/251‑1 (MQ=255)

                                                                                                                                    gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATAACGTtgtg                                                                                                                 >  2:1568975/1‑251 (MQ=255)

                                                                                                                                    gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                                                 >  1:142186/1‑251 (MQ=255)

                                                                                                                                    gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCAGCGGGCTGCCGGAGAACTGCCCTACCAGCTCTTCGGTACTTACACTAACCC‑GCCGTTTAACCCGTCCGATAATCCCGAtgta                                                                                                                 >  1:1075508/1‑250 (MQ=255)

                                                                                                                                      gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                                                                                                                                        >  2:246489/1‑211 (MQ=255)

                                                                                                                                       aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                              <  2:142120/251‑1 (MQ=255)

                                                                                                                                       aTTTGCGCCGCCACTTTGCGGGCGATGCGCTCTTTGTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                              <  2:958125/251‑1 (MQ=255)

                                                                                                                                         ttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCTGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                            <  1:188142/251‑1 (MQ=255)

                                                                                                                                         ttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCGACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGttt                                                                                                                                         >  1:552095/1‑222 (MQ=255)

                                                                                                                                               cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTTCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCCGGATCAGGTTTCGCTCCACCAGCTCATTGAGGTCCACGCGAATCGTCGGCGGGCCGACGGAGACATGCTCTACCAGACCTTCGCTACTGACA‑TAAACCTCCTGTTTAACCAATCCGATAATATCTTTTGGACCTTgtgtgt                                                                                                      >  1:1084676/1‑249 (MQ=255)

                                                                                                                                                 gcccgttGCGGGCGGTGCGCTCTTTTTCTTCGGTGTGGGTGGCCTTGGGGTCGTGCCAGGGCGTGTTGACCGAACTGTAAGGCAGCGCCGCACCGCCGTGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAGATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                    <  1:1114636/246‑1 (MQ=255)

                                                                                                                                                      ttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGGTGTGTTTTTTTCAt                                                                                               >  2:1099428/1‑251 (MQ=255)

                                                                                                                                                              gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTTTTACGTTGTGTTTGTTTCATTTTTAAAt                                                                                       >  1:1313802/1‑251 (MQ=255)

                                                                                                                                                                   gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCAGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                                                  >  2:657945/1‑251 (MQ=255)

                                                                                                                                                                      ctTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGaa                                                                               <  2:869826/251‑1 (MQ=255)

                                                                                                                                                                         tttCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                <  1:961109/120‑1 (MQ=255)

                                                                                                                                                                            cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                          >  2:661143/1‑155 (MQ=255)

                                                                                                                                                                                      ggTTGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGAACAGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgc                                                               <  1:666403/251‑1 (MQ=255)

                                                                                                                                                                                              tGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCTCACCGCCATGAT‑GG‑CGCAGGATCAGGTCTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTCTATTTATAAATACCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                       >  2:1581083/1‑251 (MQ=255)

                                                                                                                                                                                              tGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                       >  2:1165210/1‑251 (MQ=255)

                                                                                                                                                                                                cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                     <  2:1313543/251‑1 (MQ=255)

                                                                                                                                                                                                 gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                                    <  2:77500/251‑1 (MQ=255)

                                                                                                                                                                                                             gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGCCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGTATTGAGCGAACCAACAAAAGc                                        >  2:336584/1‑251 (MQ=255)

                                                                                                                                                                                                                  ttAACCGAACTGGAAGGCAGTGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGGGAATAGTCTGCGGGCTTACGGATAAATGCTCTACCAGCTCTTTGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGCGTTTGGTCCATTTATACATCCCCGGAATTCTTTTCGTTTTCGGGCATTTCACGAATCAACAAAAGcccagg                                   >  1:814743/1‑247 (MQ=255)

                                                                                                                                                                                                                  ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                                   >  1:441823/1‑251 (MQ=255)

                                                                                                                                                                                                                  ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                                   >  2:690679/1‑251 (MQ=255)

                                                                                                                                                                                                                    aaCCGAGCTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTGGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                                 <  1:690861/251‑1 (MQ=255)

                                                                                                                                                                                                                       cGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                              <  2:672372/251‑1 (MQ=255)

                                                                                                                                                                                                                           cgggAAGGCAGGACCGCACCGCGATGAG‑GG‑CGGAGGCTCGAGTTTTGCTCCGCCAGCTCATTCAGGTCGCGGCGAATTGTCTGCTGGCTGACGGAGAAAAGCTCTGGCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTATACATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCa                          <  1:740597/249‑1 (MQ=255)

                                                                                                                                                                                                                                   cAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCCGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTaaa                  >  1:670595/1‑250 (MQ=255)

                                                                                                                                                                                                                                       gccgcACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCgg              >  2:166186/1‑251 (MQ=255)

                                                                                                                                                                                                                                             ccGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                     <  1:22831/206‑1 (MQ=255)

                                                                                                                                                                                                                                              cGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtg       >  2:456608/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 cATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc    >  2:68132/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   tgat‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTc  >  1:207298/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   tgat‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTc  >  2:539641/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   tgat‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCGCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                                    <  1:225764/185‑1 (MQ=255)

TCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA‑TAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560206‑3560698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`