New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3505613	74 (0.720)	4 (0.040)	4/438	8.8	5.8%	coding (408/1086 nt)	yhfS	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
	?	NC_000913	3505821 =	62 (0.650)					coding (200/1086 nt)	yhfS	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

CGCCGGAGTAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGCACCAGCGCCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3505351‑3505613 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcaccagcgccgcaGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACAT  >  NC_000913/3505821‑3506069                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    CGCCGGAGTAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGC                                                                                                                                                                                                                                                                       <  1:173346/251‑1  gacggaagagAGCGTGGC‑CGAGATACGGTTGATAACTTCAGCGTCTCCGACCCCGGCACCGTCCCCCACGCGCCCAAATAGCTGGAAGCAGGAAAAAGTCTAGCCATTCGCGACACATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCAGCGGTTAACGCTGGAACCCCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCTTGCGGCT‑GCTGGCGCGTATGCTGCA                                                                                                                                                                                                                                                                      <  2:625790/241‑1  GCCGGAGTAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGCA                                                                                                                                                                                                                                                                      <  1:1012072/251‑1      GAGTAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAACTAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTCCATCACCCCATAGTTGTCATCGGTTAACGTTGGAACACCACCCGCGCCCAACGTTGCCAGCACACCTGCAATCCCGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGCACCca                                                                                                                                                                                                                                                                  >  2:44956/1‑249        GTAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCCTCGCCGACCACTGCACCAACACTCTCTGGTCTAACTTGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATACACAGCCGATTAGCGCCACCTTCACTACCGCATAGTTGTCATCGGTTACCGCTGGAACACTTGACGCGCGCAACCTTGCCAGCCTATCTTCCCGCACGTAGCTGTCCTGCCGCT‑GCTGGCGCGTACGCTGTACCCGCG                                                                                                                                                                                                                                                                >  2:1081356/1‑251         TAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACACCCGATTCGACCCACCTTCATCAACGCATAGTTGCCATCGGTTAACGCCGGAACACCCGCCGCGCGCAAAGTTTCCCGCCAATCCGCCAGCACGTAGCCGTCCTCCGGCTCACGGGCGCGCA‑GCTGCCCCAGCcc                                                                                                                                                                                                                                                               >  2:1038300/1‑249         TAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGACCAAATAGCTTGGAGCATGAAAATGTCGAGACATTCCCGCCGCATTCATAGTCGATTCCAGACACCTTCATCACCGCATACTTGTCATCGGTTAACGCTTGAAGACCGCCTGCGCGCCAAGTTGCCAGCCCATCTGCCAGCACGTACCTGTCCTTCCGCG‑CCCGGTGCGGATGATGCAGCAGCcc                                                                                                                                                                                                                                                               >  2:514366/1‑249                                                                                        AAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGCAC                                                                                                                                                                                                                                                                     >  1:230398/1‑167                                                                                                                                               TCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATG                                                                                                                          >  1:774763/1‑251                                                                                                                                                         ATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTG                                                                                                                                                                                                                                                                        <  2:974059/99‑1                                                                                                                                                                                             GCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCACCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGCTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGTAAATGGCGAAAAATGCTATCCACCAGCGCCAACTGCTTTGGCTGCGCCTCAATAATCGTCA                                                                            >  1:96468/1‑251                                                                                                                                                                                                                                   ccttgGGCT‑GCTGGCGCGTATGCTGGCCCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGACAGCACCGTTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGAGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTT                                      <  2:774901/246‑1                                                                                                                                                                                                                                              gataagggccaGCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGTGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAAC                            <  1:634189/240‑1                                                                                                                                                                                                                                                            gaACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTAGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTACTTAAGCCGGAACGAACAGGCCAAGCC              <  1:377319/249‑1                                                                                                                                                                                                                                                                 AGCGCCGCAGCCTGTGCGTGGAATGCATCGGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGAAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAA         <  1:302962/251‑1                                                                                                                                                                                                                                                                  GCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCGAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAA        <  1:774652/251‑1                                                                                                                                                                                                                                                                  GCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAACCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAA        <  1:408232/251‑1                                                                                                                                                                                                                                                                    GCCGAAGGCTGTGCGTGAAATGCGGCAGCCAGCACCTGTGCCAAACGCTGGGTAACGGGCGGTTGATTCAGGCGTGGCTTTAAGCCTAAATCACCGCCAGTAAGAAACTCGATGGCGGGGAAAGGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCTCCTCAATATTCGTCAGGCTATGCAGAGGAAACGTCTTCATAGGTGCCTTCCTAAAGCCGGAACGAACAGGCCAAGCCAGTAAAGA      <  2:777669/251‑1                                                                                                                                                                                                                                                                      CGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAAC    <  1:971471/251‑1                                                                                                                                                                                                                                                                       GCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACA   <  1:211394/251‑1                                                                                                                                                                                                                                                                       GCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCGATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGTCGGAACGAACAGGCCAAGCCAGGCAAGAACA   >  2:1025163/1‑251                                                                                                                                                                                                                                                                        CAGCCCGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGAAAACTGCTTTTGCTGCGCCCCAATAATCCTCAGCCTTTTCATCGGAAACGTCTTCTTAGGGTCCTTCCTTGTTCCGCAAAGCACTGGCAAAGCCAGGCAAAAACAc  >  2:822825/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    CGCCGGAGTAAAGCGTGGCGCGA‑ATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCT‑GCTGGCGCGTATGCTGCACCAGCGCCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3505351‑3505613 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcaccagcgccgcaGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACAT  >  NC_000913/3505821‑3506069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.