BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,012	1	.	G	59.7%	264.7 / 222.6	129	intergenic (‑131/+57)	frmR/yaiO	regulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand): ref base . (21/31); new base G (56/21); total (77/52)
Fisher's exact test for biased strand distribution p-value = 4.47e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 152 total aligned reads displayed.

ATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTA  >  NC_000913/379913‑380111

ataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑Gaa‑a                                                                                                          <  1:3650511/100‑1 (MQ=255)

  aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑gg                                                                                                      <  1:2569491/100‑1 (MQ=255)

   ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑ggg                                                                                                     >  1:3293893/1‑100 (MQ=255)

   ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑ggg                                                                                                     >  1:3257273/1‑100 (MQ=255)

   ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑ggg                                                                                                     >  1:4008768/1‑100 (MQ=255)

     ggggTATTCTATATGTCAATGCATAACCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑ggggg                                                                                                   <  2:1387960/100‑1 (MQ=255)

      gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑gggggg                                                                                                  >  1:1361983/1‑100 (MQ=255)

      gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑gggggg                                                                                                  >  1:3256007/1‑100 (MQ=255)

       ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑gggggg‑‑g                                                                                               <  2:317441/100‑1 (MQ=255)

        gTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATGTATCCGGAGAATGTGGTCGGATAACAAAGAG‑GAA‑A‑‑gggggg‑‑gg                                                                                              >  2:697279/1‑100 (MQ=255)

          aTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑gggggg‑‑ggg‑g                                                                                           >  2:1791880/1‑100 (MQ=255)

           ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑gggggg‑‑ggg‑gg                                                                                          >  1:559000/1‑99 (MQ=255)

            tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑gggggg‑‑ggg‑ggc                                                                                         >  1:2592981/1‑98 (MQ=255)

              tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGCATAAAATTTCTCCGGCGAATGTGGTCCGAAAACACAGCG‑GCA‑A‑‑GGGGGG‑‑GGG‑GCCaa                                                                                       >  2:773447/1‑100 (MQ=255)

                 atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCg                                                                                    <  1:426787/100‑1 (MQ=255)

                  tGTCAATGCATCCCCCCGTATAGTATATAACACTGGAGAATAAAATTTATCGGGGGAATTTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGGTGCTAATCg                                                                                    <  2:1209751/100‑1 (MQ=255)

                   gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCgg                                                                                   >  1:1147638/1‑100 (MQ=255)

                   gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCgg                                                                                   >  2:2110103/1‑100 (MQ=255)

                   gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGc                                                                                  >  2:3571237/1‑100 (MQ=255)

                   gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGc                                                                                  >  2:1291383/1‑100 (MQ=255)

                    tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCa                                                                                 <  1:2987917/100‑1 (MQ=255)

                     cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAg                                                                                >  1:2703701/1‑100 (MQ=255)

                      aaTGCATACCCCCCTATAGTATATAACACTGGTGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCAAATCGGCAg                                                                                >  2:4340089/1‑100 (MQ=255)

                      aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAg                                                                                <  1:708148/100‑1 (MQ=255)

                      aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAg                                                                                >  1:3169601/1‑100 (MQ=255)

                         gCATCCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGan                                                                            <  1:4118677/100‑2 (MQ=255)

                          naTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAg                                                                           >  2:4718545/2‑100 (MQ=255)

                          cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGaa                                                                            >  2:4961349/1‑100 (MQ=255)

                          cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAg                                                                           >  2:2742710/1‑100 (MQ=255)

                          cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAg                                                                           <  2:295100/100‑1 (MQ=255)

                             aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGgc                                                                         >  2:1419277/1‑100 (MQ=255)

                              ccccccTATAGTATATAACACTGGAGAATAAGATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGgcc                                                                        >  1:819017/1‑100 (MQ=255)

                              ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGgc                                                                         >  1:4792664/1‑100 (MQ=255)

                              ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGgc                                                                         >  1:2283491/1‑100 (MQ=255)

                              ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGgc                                                                         >  2:4176201/1‑100 (MQ=255)

                                 cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGgccgcc                                                                     >  2:4210647/1‑100 (MQ=255)

                                  ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGcccc                                                                   <  2:2566445/100‑1 (MQ=255)

                                   cgataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCg                                                                  <  1:3610722/98‑1 (MQ=255)

                                      taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                               <  1:1328014/100‑1 (MQ=255)

                                         tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                             >  1:1222872/1‑100 (MQ=255)

                                         tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGCCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                            <  2:1552290/100‑1 (MQ=255)

                                          atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                             >  1:1155983/1‑100 (MQ=255)

                                           tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                           >  2:2445379/1‑100 (MQ=255)

                                           tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                          >  2:908991/1‑100 (MQ=255)

                                           tagaaCACCGGATAATAAAATTTATGAGCGTAATGTGGTCGGAATACAAAGGGTGAA‑G‑‑GGGGGG‑‑GGG‑TCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                           <  2:4426342/97‑1 (MQ=255)

                                             taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                         >  2:3803539/1‑100 (MQ=255)

                                             taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                         >  2:1429254/1‑100 (MQ=255)

                                             taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCGAATCGGCTGGGAAGGCCCGCCCGGAAAacggg                                                         >  1:4581507/1‑100 (MQ=255)

                                              aaCACTGGAGAATAAAATTTATCCGGTGAAGGGGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                       <  1:3201995/100‑1 (MQ=255)

                                               acacTGGAGAATAAAATTTATCCGGTGAGCGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGCTAgcgggcg                                                       >  2:2923820/1‑100 (MQ=255)

                                               acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                       >  1:3769599/1‑100 (MQ=255)

                                                cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                      >  1:4499988/1‑100 (MQ=255)

                                                 attGGAAAATAAAATTTATCCGGTAAATGTGGTCGGAAAACAAGGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     <  1:3811499/98‑1 (MQ=255)

                                                 acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     <  1:2764134/100‑1 (MQ=255)

                                                   tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                                   >  1:4682774/1‑100 (MQ=255)

                                                   tGGAGAATAAAATTTAGCCGGTAAATGTGGGCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGA‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                                  <  1:3336598/100‑1 (MQ=255)

                                                     gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                <  2:2009315/100‑1 (MQ=255)

                                                     gagaATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                <  2:441225/100‑1 (MQ=255)

                                                      agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGGGCGGG‑GCTAATCGGCAGAGAAGGCCCCCCCGGAGAGCGGGCGGCAGaa                                                 >  2:1056355/1‑100 (MQ=255)

                                                       aaaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                <  1:4229501/99‑1 (MQ=255)

                                                         aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGagatcg                                             >  1:4461335/1‑95 (MQ=255)

                                                         aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                             >  1:4951186/1‑100 (MQ=255)

                                                          tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                           >  1:5093993/1‑100 (MQ=255)

                                                          tAAAATTTATCCGGTGAATGGGGGCGGAAAACAAAGGG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                            <  1:4038536/100‑1 (MQ=255)

                                                           aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                          >  2:4675067/1‑100 (MQ=255)

                                                           aaaaTTTATCCGGTGAATGTGGGCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                           <  2:1129395/100‑1 (MQ=255)

                                                            aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                           >  2:3799826/1‑100 (MQ=255)

                                                             aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                         >  1:5037555/1‑100 (MQ=255)

                                                               tttATCGGTTGAATGGGGACGGAAAAAAAAGGG‑GAAGG‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                       <  1:2758915/100‑1 (MQ=255)

                                                                ttATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                     <  1:923618/100‑1 (MQ=255)

                                                                 tATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt                                    >  1:3062997/1‑100 (MQ=255)

                                                                     cGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                  >  1:2053532/1‑100 (MQ=255)

                                                                        tGAAGGTGGGCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                              <  2:428841/100‑1 (MQ=255)

                                                                          aaTGTGGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGCTAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                            >  1:2185279/1‑100 (MQ=255)

                                                                             gtgGTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCAAATCGGCAGGAAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGaa                         >  2:1905617/1‑99 (MQ=255)

                                                                              tgGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                       >  1:4507023/1‑100 (MQ=255)

                                                                               ggTCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGAGAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg                        >  2:4964671/1‑100 (MQ=255)

                                                                               ggTCGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                       >  2:3239243/1‑100 (MQ=255)

                                                                                ntCGGAAAACAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCGGGGAAGGCCGCCCCGGATAGCGGGGGGCGGAAGGAATCAGAATTTCCAGGTCAGACgg                       >  1:419421/2‑100 (MQ=255)

                                                                                  cGGAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggtct                    >  1:300697/1‑97 (MQ=255)

                                                                                    gAAAACAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                  <  1:1440532/100‑1 (MQ=255)

                                                                                    gAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                 <  2:2673084/100‑1 (MQ=255)

                                                                                      aaaCAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                >  1:2181141/1‑100 (MQ=255)

                                                                                        agagagag‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt              <  2:4032358/96‑1 (MQ=255)

                                                                                        aCAAAGAG‑GAA‑AGGGGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg               >  1:2401058/1‑100 (MQ=255)

                                                                                        aCAAAGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt              >  2:2949638/1‑100 (MQ=255)

                                                                                         cAAAGCG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg            <  2:2175366/100‑1 (MQ=255)

                                                                                          aaaGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg            >  2:1375621/1‑100 (MQ=255)

                                                                                           nagag‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa          >  1:4433634/2‑100 (MQ=255)

                                                                                           aaGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc           >  1:1430758/1‑100 (MQ=255)

                                                                                           aaGAG‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc           >  1:1664254/1‑100 (MQ=255)

                                                                                            agag‑GAA‑G‑‑AGGGGG‑‑CGA‑ACTAATCAGCAAGTAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg         <  1:5165064/100‑1 (MQ=255)

                                                                                            agag‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa          >  1:2636628/1‑100 (MQ=255)

                                                                                            agag‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg         <  1:1744287/100‑1 (MQ=255)

                                                                                              ag‑GAA‑AG‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa        <  1:1400715/100‑1 (MQ=255)

                                                                                              ag‑GAA‑A‑‑‑GGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc      <  2:2007058/100‑1 (MQ=255)

                                                                                               g‑gAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc      >  1:1642858/1‑100 (MQ=255)

                                                                                               g‑gAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc      >  2:297933/1‑100 (MQ=255)

                                                                                                 gAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGCCCg     <  2:1754355/100‑1 (MQ=255)

                                                                                                     a‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTa  <  2:3964411/100‑1 (MQ=255)

ATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAG‑GAA‑A‑‑GGGGGG‑‑GGG‑GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTA  >  NC_000913/379913‑380111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`