| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,782,913 | A→C | 22.0% | V250G (GTG→GGG) | gpsA ← | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,782,913 | 0 | A | C | 22.0% | 96.7 / 10.0 | 73 | V250G (GTG→GGG) | gpsA | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Reads supporting (aligned to +/- strand): ref base A (28/28); new base C (3/14); total (31/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGC > NC_000913/3782678‑3783149 | tAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCa < 2:1162383/239‑1 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCCCC‑ACCGGGTGTCGG‑GTCCGGGAAGCACCAGaaccccc > 1:1037945/1‑233 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑CCCGGTTGTCGG‑TTCCGGGAAGCACCACATCG‑ccc > 1:505674/1‑238 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCG‑CCCGGGTTTTGG‑TACCGGTAAACACCCGATTG‑ccc > 1:616706/1‑238 (MQ=255) cAATGCTGCGTGGCGGTCTTTTTTCCCGAAATATAAACCTTGAAAATTTCCCTGGTTTATTGGCATTCCAACGCCG‑AGCCGTGCCCCCGTTTCGCGGCTTTCTTTCGTATTGCGGTACCCTCCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACACCCAGGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCCCCAGATCG‑cc < 2:616706/237‑1 (MQ=255) cAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCa < 1:812018/238‑1 (MQ=255) tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCC‑ACTGGTTGTCGG‑GACAGGTAAGAACCCAAACG‑CCa‑aac > 1:484379/1‑236 (MQ=255) ccggccgtgTTTTTCCCGCAATATAATCCTTGAAAAATTTCCTGTGTTTTTGGCATTTCAGCGCCGAACCGATGCCCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGGGCATCATGCCAAAACGGCGGTTCCGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCAt < 2:944065/231‑1 (MQ=255) cgcgTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGGTTAGCGCCCGGGTTTTGGG‑GACGGGAAAGCCCCAAAAGC‑CCA‑AACCCCCCCATGccc > 2:147341/1‑239 (MQ=255) gTTTTTCCCGAAATAAAATCCTTAAAAATTTTCCTCGGTTATTGGCATTTCAACTCCGAAGCGATGCCCCAG‑TTCGCGGCCTTCTTTCGTATGGCGGTAGCCTTCCCCCCCCGGACCAATCTTCCCCGGCGCGCTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATaa < 2:1037945/239‑1 (MQ=255) atatAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGGAAGAACCAGATAG‑CCC‑AGCCCCCCCCCGCCCCTACAAGTGTGAGGGt > 2:155776/1‑239 (MQ=255) tataATACTTGAAAATTTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCAt < 1:1051276/218‑1 (MQ=255) tagaataCTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCAt < 1:536292/215‑1 (MQ=255) ataatcCTTGATAATTTCCCCCGTTTTTTGACATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTACCCTTCCCCCCCCTGCCCAATCTTCTCCTGCGCGTTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCCCCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑Cg < 2:484379/239‑1 (MQ=255) acccTTGATAATTTCCCATGGTTTTTGGCTTTACAACGCCGAAGCGATGCCCCTG‑TTCGCGGACTTCTTTCGTTTTGCGGTAGCCTTCCCCCACCGGCCCAATCTTCCCCGGCGCGTTTTGTACCTCCATCCCCTGCCCGGGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAAACCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCa < 2:669049/236‑1 (MQ=255) tcTTGATAATTTCCCTCGGTTGTTGCCATTTCACCCCCGAAGCGATCCCCCAG‑TTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGCCCAATCTTCCCCTGCGCGCTTTGTACACCCATGCCCTGCCCGACCATCATGCCAAACCGGCGGTTCCGCG‑CTTGTTTGTCGG‑TACAGGTAAGCCCCAGATCC‑CCA‑ACCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCAcc < 2:176200/238‑1 (MQ=255) aCTAGATAATTTGCCTCGGTTTATGGCGTTTCACCGCAGAACCGATGCGCCAG‑TTGGCGGCCTTCTTTCGTATTGCGGTACCCTCCCCCCCCCTGCCCAATCGTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGCCCAAAACGGCGGTTACGCA‑ACTGGTTGTCGG‑TACGGGTAAGCACCAGACCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCAcc < 2:176547/239‑1 (MQ=255) ttGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCC‑ACTGGGTGTCGG‑TACAGGGAAGCACCCGATCG‑CCA‑AGACCCCCCATTCCCCCAAAGGGGGCAGGGT‑CGGCAccc > 1:837181/1‑238 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑CCTGGTTGTCGG‑GTAAGGGTAGAACCCAAACC‑CCACACCCCCCCCATCCCCCAAAAGG‑GGCAGGGT‑CGGGAccccc > 1:1022929/1‑236 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑CCTGGTTGTCGG‑GACAGGGAAGCCCCCAAACG‑CCA‑AACCCCGCCATGCCCCTACAAGTGGCAGGGG‑CGGCACCCac > 1:425071/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTTTCGG‑TACAGGGAAGCACCAGATCG‑CCC‑AACCCCGCCCTGCCCCTAAAGGGGGACGGGG‑CGGGAcccca > 1:176547/1‑236 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AACCCCGCCCATCCCCTAAAGGTGGCAGGGT‑CGGCACCCAgc > 1:858266/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACCTGGTGTCGG‑GACAGGTAAGCACCAGAAAG‑CCA‑AACCCCCCCATGCCCCTAAAGGGGGCCAGGG‑CGGGACCCAgc > 1:632862/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACCGGTTGTCGG‑TACAGGGAAGCACCAAATCG‑CCA‑AGCCCCGCCACGCCCCCAAAAGGGGCAGGGT‑CGGCACCCAgc > 1:318431/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCG‑ACTGGTTGTCGG‑GTCAGGTAAGGACCAGAACT‑CCC‑AACCCCGCCATGCCCCCCAAGAAGGCAAGGT‑CGGccgcccc > 2:468665/1‑232 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCG‑ACTGGTTGTCGG‑GACAGGGAAACCACAAAACG‑CCA‑AACCCCCCCATGCCCATAAAAGTGGCAGGGT‑CGGCACCCCgc > 1:283221/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCG‑ACTTGTTTTCGG‑GACAAGGAAGCACCCGAATG‑CCA‑AACCCCCCCCAGGCCACTAAAGTGGCAGGGG‑CGGCACCCac > 1:669049/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGGTACGCG‑ACTGGGTGTCGG‑TACAGGTAAGAACCAAAACG‑CCA‑AGACCCGCCATGGGCCTAAAAGTGGCAgggggcgggccccc > 2:365181/1‑227 (MQ=255) aaaaaTTTCCGGGGTTATTGCCATTCAAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCAGGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAgcg < 2:425071/237‑1 (MQ=255) ttCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCg < 1:802272/197‑1 (MQ=255) tATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGAcgcg < 1:365181/239‑1 (MQ=255) ttCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCG‑CCTGGTTGTCGG‑GGCAGGGAAGGACCAGAAGGCCCA‑ACCCCCCCCAGCCCCCTAAAGGGCGGAGGGTGGGGCCCCCAGCGCCCCCCAAAGCcgcacctact > 2:1133558/1‑231 (MQ=255) tCACCGCCGAAGCGATCCGCAAT‑TCCCCGGCTTTCTGCGGTATTGCGGTAGCCTTCCCCCACCGGACCAATCTTCTCCTGCGCGTTTTGTACATCCATGCCCGGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TAAAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc < 2:505674/239‑1 (MQ=255) gCGATGCCCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACgg < 1:159351/238‑1 (MQ=255) tCGGGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCCCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAgcg < 1:1241212/235‑1 (MQ=255) tCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAgcgc < 1:434305/236‑1 (MQ=255) ggATTTCGTGCGTATTGGGGTAGCCTTCCACCCCCTGACTAATCTTCCCCTGCGCGTTTTGAACTTCCAGGCCCGGACCGAGCATCAGGCCAAAACGGCGGTTACGCG‑ACGGGTTGTCGG‑TACAGGTAAGCACCAGACCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTAcg < 2:862122/238‑1 (MQ=255) gTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCAAAAACGGCGGTTCCGCG‑ACTGGTTGTCGG‑TACAGGTAAGCCCCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc < 1:468665/239‑1 (MQ=255) tATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCCCCTGCGCGTTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCCCCAGATCG‑CCA‑AGCCCCGCCATGCCCATaaa < 2:488724/151‑1 (MQ=255) tATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTATCC‑CCCGGGTGTCGG‑TACAGGTAAGCACCAAAACG‑CCA‑AACCCCCCCCTGCCCCTCAAAGGGGCCGGGG‑CGGGACCCAGCGCCGCACCCACACGCGACCTTTTATCCCGCCCACCGGCGGTCAAGGCCGATCgcgccgtcgtcccc > 2:1010800/1‑229 (MQ=255) ggTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCAcc < 1:330607/163‑1 (MQ=255) gCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTTTCGG‑GACAGGGAAGCACCAGAACG‑CCA‑AACCCCCCCCTGCCCCTAAAAGGGGCAggg > 1:488724/1‑151 (MQ=255) ccTTCCACCACCGGACCAATCTTCCCCGGCGCGTTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATg < 2:259762/132‑1 (MQ=255) ccacccCCTGCCCAATCTTCCCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGGCGG‑TACAGGTAAGCACCAGACCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAgcg < 1:438757/204‑1 (MQ=255) cccTGACCAATCTGCCCCTGCGCGCTTTGTACATCAAGGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACGGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa < 1:529693/235‑1 (MQ=255) ccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGGTGTCGG‑GACAGGGAAGCACCAGATCG‑CCA‑AGCCCCCCCCTGCCCCTAAAgg > 1:259762/1‑132 (MQ=255) tGACCAATCTTCCCCTGCGCCTTTTGTCCATCCATGCCCTGACCGAGCATCATCCCAAACCGGCGTTTCCGCG‑ACTTGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCTCCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATccc < 1:995961/239‑1 (MQ=255) cccACTCTCCACCTGCGCGCTTTGTCCATCCATGCCCTAACCGGGCTGCATGCCAAAGCGGCGGTCCCGCG‑ACTGTTTTCCGG‑TACGGGTAAGCCCCAGACCC‑CCA‑GGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCCCCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCg < 2:318431/238‑1 (MQ=255) aCCAATCTGCCCCGGGCCGTTTTGTACATCCACTCCCTACCCGACCATCATGCAAAAGCGGCGGTTCCGCG‑ACTGGTTGTCGG‑TACAGGTAAGCCCCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCg < 2:285254/239‑1 (MQ=255) aaTCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCG‑ACCTGTTTTCGG‑GACAGGTAAACACCAAAACG‑CCA‑AGCCCCGCCCTGCCCCTAAAAGGGGCACGGG‑CGGGCCCCCGCGCCCCCCCCACACGCGGAACTTCAGCCAGACCACGCGCGCTCAACCCCCGACGCGCACTCGCACCAAAACACAAACCGTCGGGCagtcccaca > 2:687014/1‑230 (MQ=255) ttttctCCTGCGCGTTTTGTCCATCCATGCCCTGCCCGACCATCATGCAAAACCGGCGGTTCCGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc < 1:824167/206‑1 (MQ=255) tcttctCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGGCAAAAGCGGGGGTTACGCG‑ACTGGTTGTGGG‑TACAGGTAAGCCCCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc < 1:588800/169‑1 (MQ=255) aTCCATGCCCTGACCGAGCATCAGGCCAAAACGGCGGTTACGCG‑ACGGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg < 1:1133558/239‑1 (MQ=255) ccATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcgcg < 1:888145/239‑1 (MQ=255) ccATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCCCCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGa < 1:989866/131‑1 (MQ=255) ccTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCCCCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCa < 1:147341/239‑1 (MQ=255) aCCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑GACAGGGAAGCACCAAAACG‑CCC‑AGCCCCCCCATGCCCCTAAAGGGGGCCGGGG‑CGGCCCCCCGCCCCCCCCCCAAACGCGGCAttt > 2:989866/1‑131 (MQ=255) aCCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGGTGTCGG‑GACAGGTAAACACCAAAACG‑CCC‑AACCCCCCCCCGCCCCTAAAAGGGGGAGGGG‑CGGCACCCCGCGCCCCCCCAAGAACCCACCTTTTAGCCCCCCCCCCGGGGAGCAGCGCCGGACGCGCCTTCGCACCCAAACCGATACCCCCGGACCTCCCCGCCCCCCTGGCCACAAAGTATTTTATCGCgccgccccgc > 2:886736/1‑234 (MQ=255) cggAGCATAACGCCAAACCGGCGGTTCCGCG‑CCT‑GTTGGCGGTTACAGGTAACCCCCAGCCCG‑CAA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg < 2:858266/237‑1 (MQ=255) cGAGCATCATGCCAAAACGGGGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg < 1:1150249/238‑1 (MQ=255) aGCATCATGCCAAAACGGCGGTTACGCG‑GCTGGTTGTCGG‑GACAGGTAAGCACCAGAACG‑CCA‑AGCCCCCCCCTGCCCCTAAAGGGGGCAGGGT‑CGGCACCCAACGCCCCCCCCAGACGCGACATTTCAGCCAGCCCCCGGGTGATCAGCGCCCTACCCGCCCTCGCACCCAAACAGCCAACCTCACAAATCCCCTCCACAATAGCCATAACGGATTTTACAGCGCCGCCaccagtca > 2:847472/1‑231 (MQ=255) gCATCATGCAAAAACGGCGGTTACGGG‑ACTGTTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg < 1:1176027/235‑1 (MQ=255) catcatGCCAAAGCGGCGGTTCCGCG‑ACTGGTTGCCGG‑TACAGGAAAGCCCCAGACCC‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACg < 1:513776/238‑1 (MQ=255) atGCCAAACGGGGGTTTCCGCG‑CCTGGTTGGCGG‑TACAGGTAAGCCCCAGATCC‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAt < 1:155776/239‑1 (MQ=255) ggccttcccgcg‑TCTGGTTGTCGG‑AACAGGAAAGCACCAGACCG‑CCA‑ACCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCCCCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa < 2:283221/231‑1 (MQ=255) gTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGa > 1:5143/1‑239 (MQ=255) cAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc > 1:1141603/1‑239 (MQ=255) cAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGc > 1:1228092/1‑238 (MQ=255) gggTAAGCCCCAGACCC‑CCC‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg < 1:1075470/238‑1 (MQ=255) aGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCgccgcc > 2:1054638/1‑187 (MQ=255) aGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCgccgcc < 1:1054638/187‑1 (MQ=255) aGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGCCATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGt > 1:542455/1‑238 (MQ=255) cACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgc > 2:486184/1‑238 (MQ=255) | TAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAG‑TTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCG‑ACTGGTTGTCGG‑TACAGGTAAGCACCAGATCG‑CCA‑AGCCCCGCCATGCCCATAAAGGTGGCAGGGT‑CGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGC > NC_000913/3782678‑3783149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |