| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 458,201 | 0 | C | T | 27.5% | 90.1 / 12.4 | 51 | S259F (TCC→TTC) | clpX | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease |
| Reads supporting (aligned to +/- strand): ref base C (8/29); new base T (14/0); total (22/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTAT > NC_000913/457976‑458436 | aTGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTCCGGGCTGGATAAAGTGG‑TTTTCCACCGGGTa > 1:39169/1‑236 (MQ=255) tGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTGTTTCGGTGTGGTGGAAATGA‑TTTCTCACCgtgtt > 2:685546/1‑234 (MQ=255) tGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGATTATTTGTGGCGGTGTGGTTTCCGGTCTTGGTCAAGTGAGTTTTCCAcgcgga > 2:442333/1‑230 (MQ=255) aaGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTc < 1:376413/236‑1 (MQ=255) gATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCAc < 1:471610/217‑1 (MQ=255) tttCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGc < 2:6143/236‑1 (MQ=255) ctcGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑t < 1:670063/235‑1 (MQ=255) aGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCTGGATAAAGAGA‑TTTTCCACCGTGTAGAAAACGGCTCCGGCATTTG‑GTTTTgtg > 1:584077/1‑234 (MQ=255) ccATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGCTTGTCGGGCTGGGTAAACAGA‑TTTTCCACCGCGTTGACAACGGCTCCGGCA‑TTGTGTTTTGG‑GCGACggcgcaa > 1:147194/1‑231 (MQ=255) ttgcccGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑g < 1:157816/233‑1 (MQ=255) gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGGCTAGATAAAGTGA‑TTTTCCACCGTGTAGAAAACCGCTCCGGCA‑TTG‑GTTTTTGTGTGACCGTCAAAGC‑GTATTcc > 2:662577/1‑236 (MQ=255) gaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCGGTCTTGATAAAGTGA‑TTTCCCACCCGGTAGAAACCGGCTCCGGCA‑TTG‑GGTTTTGCGCGACGGTAAAAGC‑GAAATCCgc > 1:160491/1‑235 (MQ=255) agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGTCGGGCTGGGTAAAGTGA‑TTTTCCACCGTGGAGAAACCGGCTCCGGCC‑TTG‑GTTTTTGCGCGACGGTTAAAAC‑GAAGTCCGCCAAA‑GCAAGCGAAggggg > 1:149782/1‑226 (MQ=255) aaCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGt < 1:469773/232‑1 (MQ=255) agggTACGGTAGCAGCTGTTCCACCGCAAGGTGGGCGTAAACATCAGCAGCAGGAATTCTTGCAGGTTGATACCTATAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt < 2:719001/234‑1 (MQ=255) cGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGATAAAGTGC‑TTTTCCCCCCGGTTGAAACCGGGTCCGGCC‑CTG‑GGTTTTGCGGCGCGGGAAAAAC‑GAAGGCCCACAAACAAAAGCG‑AGGCGGACTGCTGGGGCTGGTTTGACTGGAAGaaac > 2:549590/1‑229 (MQ=255) ggTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt < 1:187446/234‑1 (MQ=255) ctgctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt < 1:449510/235‑1 (MQ=255) tgctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTAGTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGttt < 2:565309/235‑1 (MQ=255) gTTCCACCGCAAGGTTGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt < 1:129883/236‑1 (MQ=255) tCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATc < 1:704824/223‑1 (MQ=255) tCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGGTAAAGTGA‑TTTTCCACCCTGTAGAAAACGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAAC‑GAAGTCCGACAAA‑GCAAAGGAAGAGGGGCGGCTGGGGCGGGGTGAACAGGGAGAACTGTTCAAGATTggggtg > 2:631359/1‑231 (MQ=255) aCCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATc < 1:662577/235‑1 (MQ=255) ccGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGTGTTTGCCGGTCTGGATAAAGTGA‑TTTTCCCCCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGGGCAGGGTGAACCGGAGGAACTGATCAAGCTTGGGCTCTTccc > 2:158525/1‑236 (MQ=255) ccGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGTGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc < 1:158525/236‑1 (MQ=255) acgtaaGGGGGGCGTGAACCTTCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc < 1:652902/232‑1 (MQ=255) gCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTg < 1:262912/236‑1 (MQ=255) ccGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCCGGTCTGGATAAAGTGA‑TTTTCCACCGTGTAGAAACCGGCTCCGGCC‑TTG‑GTTTTTGCGCGACGGTAAAAGC‑GGAGGCCGACAAA‑ACAAGCGAAGGCGGGCTGCTGGCGCAGGTTGAACCGGGAGAACTGATCAAGATTTGTCTTATTCCTCA‑GGTTTTTTGTAGGCTGcc > 2:588697/1‑236 (MQ=255) cagaagGAATGCTGGCAGGTTGATCCCTCTAAGATCCTGTTTAGTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGt < 2:149782/236‑1 (MQ=255) cagGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTCCGGTCTGGGTAAAATGA‑TTTTCCACCGTGGAGAAAACGGCGCCCGCA‑TTG‑GTTTTTGTGGGGCGGGAAAAACGGAAGACC‑ACAAA‑GCAAACGAAGGCGGGGGGCTTGCGACGGGTGTACAGGAAGAATACAGCAAGTTTTGTCTGATACCTCAGGTTTAtgtgcggttg > 2:572366/1‑219 (MQ=255) gAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATccc < 1:516619/206‑1 (MQ=255) tCTTGCAGGTTGATACCTCTAAGATCCTGTTTAGTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAAc < 1:588697/235‑1 (MQ=255) cTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGttt < 2:168442/190‑1 (MQ=255) cTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGGTGAACCGGAAGATCTGATCAAGttt > 1:168442/1‑190 (MQ=255) tGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCTGGGTAAAATGA‑TTTCCCACCGGGGAGAAACCGGCTCCGGCC‑TTG‑GTTTTGGCGCGACGGTAAAAAC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGGGGTGCTGGCGCCGGTTGGACCGGGAGATGTGATCAAGGTTGGTCTTATTCCTGA‑Ggtt > 2:516619/1‑206 (MQ=255) atccttctAAGTTCCTGTTTATTGGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaatgaa < 1:146869/228‑1 (MQ=255) aCCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAg < 1:631359/236‑1 (MQ=255) ctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGGTAAAGTGA‑TTTTCCACCGGGTAGAAAACGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGGAAAAGC‑GAAGGCCGACAAA‑GCAAGCGAAGGCGAGGTGCTGGCGCCGGGTGAAACGGGAGAGCTGATTAAGATTGGGCTTATCCCTCA‑GTTTATTGGTGGTGTGCCGGTTGTTGCGACGTTGTATGAACTGataggt > 2:391999/1‑231 (MQ=255) aaGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCgg < 1:15903/154‑1 (MQ=255) tCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGct < 2:584077/236‑1 (MQ=255) tCCTGTTTATTTGTGGCGGTGCGTTGGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGTCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCTGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGct < 1:282097/236‑1 (MQ=255) cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAATGA‑TTTTCCACCGTGTAGAAAACCGCTCCGGCA‑TTG‑GGTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGGTTTGGCTTATCCCTTA‑GTGTATTGGTCGTCTGCCGGTTGGCGCCACGTTTAATTAAACTAGCGAAGAAGgtct > 2:419735/1‑236 (MQ=255) aTTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt < 1:201427/171‑1 (MQ=255) ttGTGGCGGTGGGGTTGCCGGTCTGGATAAAATGA‑TTTTCCACCGTGTAGAAAACGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGGTGAACCGGGAGATATGATCAAGTTTGGGCTTATTCCTGA‑GGTTATTGGGCGTCTGCCCGTTGTCTGAACGTTGAATGAACTGAGCGaacaagacgtggattc > 2:255611/1‑224 (MQ=255) tttGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGc < 1:391999/235‑1 (MQ=255) tAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGa < 1:419735/236‑1 (MQ=255) tttCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGAc > 2:644406/1‑229 (MQ=255) tttCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCa > 1:582207/1‑235 (MQ=255) tttCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAg > 1:87508/1‑236 (MQ=255) tttCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAg > 2:693550/1‑236 (MQ=255) ttCCCACCGTGTAGAAACCGGCTACGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCNNCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCa < 1:583162/234‑1 (MQ=255) tgcccACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAg < 1:172848/230‑1 (MQ=255) cccACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTAt > 2:499194/1‑236 (MQ=255) | ATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGA‑TTTCCCACCGTGTAGAAACCGGCTCCGGCA‑TTG‑GTTTTGGCGCGACGGTAAAAGC‑GAAGTCCGACAAA‑GCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGA‑GTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTAT > NC_000913/457976‑458436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |