Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,713	A→C	N553K (AAT→AAG)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,713	0	A	C	100.0%	257.9 / NA	73	N553K (AAT→AAG)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/38);  total (35/38)

GTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAAC  >  NC_000913/4285480‑4285941                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       gTCAAGGTACCGCCCGGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCtt                                                                                                                                                                                                                                      <  1:214928/236‑1 (MQ=255)    gAGGTATCGCCCAGTTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgt                                                                                                                                                                                                                                    <  1:575938/235‑1 (MQ=255)         tcccccgtgtttGCTGGTAGCGGCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGa                                                                                                                                                                                                                               <  2:519171/225‑1 (MQ=255)          tCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAAt                                                                                                                                                                                                                             >  2:259473/1‑236 (MQ=255)           cGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAAt                                                                                                                                                                                                                             <  1:277549/235‑1 (MQ=255)               gaGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTGTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGa                                                                                                                                                                                                                               <  1:93909/228‑1 (MQ=255)                  gTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctacta                                                                                                                                                                                                                     >  1:677969/1‑236 (MQ=255)                       tGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggc                                                                                                                                                                                                                 >  2:613150/1‑235 (MQ=255)                            tCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGa                                                                                                                                                                                                                               >  2:285481/1‑216 (MQ=255)                            tCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGa                                                                                                                                                                                                                               <  1:285481/216‑1 (MQ=255)                                ccgccgCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGCATACCTACTACGGCGGCTTCgg                                                                                                                                                                                                         >  1:97005/1‑234 (MQ=255)                                   ccgcAATTTGGCGCAGAATCCGGCGAATAATTTGGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCgg                                                                                                                                                                                                         <  2:637817/231‑1 (MQ=255)                                         ttttGCGCAGAATTCGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGa                                                                                                                                                                                              <  1:761885/236‑1 (MQ=255)                                                cAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATAc                                                                                                                                                                                                                           >  1:455564/1‑200 (MQ=255)                                                cAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATAc                                                                                                                                                                                                                           <  2:455564/200‑1 (MQ=255)                                                       cGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgcg                                                                                                                                                                                 <  1:613150/235‑1 (MQ=255)                                                       cGGCACATGATTTGCCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGGCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCATGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgcgc                                                                                                                                                                                <  2:708953/236‑1 (MQ=255)                                                             aTAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACt                                                                                                                                                                           >  1:507657/1‑235 (MQ=255)                                                                  tttGCCGGAGCGGGTTTTAGGCAGGGACTCGGTCCAGGACAGCACGTCTGGCGTCGCCCGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAtct                                                                                                                                                                     <  2:507657/236‑1 (MQ=255)                                                                                                ggTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑g                                                                                                                                       >  1:113103/1‑235 (MQ=255)                                                                                                  tCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTc                                                                                                                                    <  1:547203/236‑1 (MQ=255)                                                                                                      gTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAAAC‑GTTCAGc                                                                                                                                 >  1:653635/1‑235 (MQ=255)                                                                                                            caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGa                                                                                                                                                                                              <  1:398486/169‑1 (MQ=255)                                                                                                            caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGa                                                                                                                                                                                              >  2:398486/1‑169 (MQ=255)                                                                                                            caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATTCGCCACCAGCGCCGACCCAATATCTGCCGTCCCCACACCGTGACCGGAGAC‑GTTccacaacacg                                                                                                                           >  2:414663/1‑226 (MQ=255)                                                                                                            caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCAcgtcg                                                                                                                           >  2:334277/1‑235 (MQ=255)                                                                                                            caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCAcgtcg                                                                                                                           >  1:151441/1‑235 (MQ=255)                                                                                                                 cTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTGCCCAGACGGTGACCGGAGAC‑GGTCTGCATGTCTGCcac                                                                                                                      >  2:391602/1‑235 (MQ=255)                                                                                                                 cTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCcac                                                                                                                      >  1:502877/1‑235 (MQ=255)                                                                                                                     cGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGcc                                                                                                                 <  2:113103/236‑1 (MQ=255)                                                                                                                     cGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGcc                                                                                                                 <  1:334277/236‑1 (MQ=255)                                                                                                                         gCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCgg                                                                                                              <  2:369435/235‑1 (MQ=255)                                                                                                                          ccAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGc                                                                                                                                                                 >  1:278221/1‑184 (MQ=255)                                                                                                                          ccAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGc                                                                                                                                                                 <  2:278221/184‑1 (MQ=255)                                                                                                                            aGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTAt                                                                                                          <  1:259473/236‑1 (MQ=255)                                                                                                                                  ccAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTTACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATc                                                                                                         <  1:324405/231‑1 (MQ=255)                                                                                                                                    aaTCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCGCACGCCCGGTTATCCAGTaa                                                                                                   <  2:151441/235‑1 (MQ=255)                                                                                                                                     aTCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTAACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATa                                                                                                 >  1:170064/1‑236 (MQ=255)                                                                                                                                                 aCCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCAt                                                                                      >  1:152922/1‑235 (MQ=255)                                                                                                                                                           ggACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAcg                                                                               <  1:414663/232‑1 (MQ=255)                                                                                                                                                             aCTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACg                                                                                                                   <  1:614583/194‑1 (MQ=255)                                                                                                                                                             aCTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACg                                                                                                                   >  2:614583/1‑194 (MQ=255)                                                                                                                                                             aCTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGAGCCACCAGCGCCGACTCAATCTCTGCCGTCCCC‑GACCGGGGACGGGGACAGTTCAGCAGCTGGTCCTCCGCCCCGGTTTTCCAGGAATAGCAATCTTCATCCCGActggtca                                                                          >  2:140905/1‑229 (MQ=255)                                                                                                                                                                 cTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCCGACGGGTGCCGGAGAC‑GGTCAGCACGCCGTCCCCACGCCCGGTGATCCCGGAAAAGCCATCTTCACTGCGAGCAGCGCCG‑Tc                                                                     >  2:195439/1‑235 (MQ=255)                                                                                                                                                                                     ggTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCCCGTCGTCCACCCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACCCCCGCCG‑TCGCCGCTGAACTACATAttttt                                                >  1:339222/1‑236 (MQ=255)                                                                                                                                                                                            ccccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTg                                         >  1:128751/1‑236 (MQ=255)                                                                                                                                                                                                gTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTg                                         <  1:394090/232‑1 (MQ=255)                                                                                                                                                                                                 tGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATTCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa                                    >  2:642231/1‑236 (MQ=255)                                                                                                                                                                                                 tGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa                                    >  1:151003/1‑236 (MQ=255)                                                                                                                                                                                                  taTTAAGCGTGACGGAGGCGTAGATCGCCTGACCTTTAATCTTGGGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAg                                   <  2:677969/235‑1 (MQ=255)                                                                                                                                                                                                       aGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGt                              <  1:304175/236‑1 (MQ=255)                                                                                                                                                                                                       aGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGGTATCCAGTAATAGCCATCTTCGTAGCGACGCGCCCCGCACGCCG‑TGCACTACAAATTTTTGAAgggtgggaagg                                  >  2:394090/1‑223 (MQ=255)                                                                                                                                                                                                        gCGTTACGTGGGCGTAGCTCCCATGACCTAGAATCTGGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGt                              <  2:97005/235‑1 (MQ=255)                                                                                                                                                                                                        gCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTc                             <  2:151003/236‑1 (MQ=255)                                                                                                                                                                                                           tGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCcaca                                                                                                                     >  1:621416/1‑146 (MQ=255)                                                                                                                                                                                                           tGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCcaca                                                                                                                     <  2:621416/146‑1 (MQ=255)                                                                                                                                                                                                             aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGTTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGaa                                             >  2:709784/1‑215 (MQ=255)                                                                                                                                                                                                             aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGTTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGaa                                             <  1:709784/215‑1 (MQ=255)                                                                                                                                                                                                              cGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGt                                          >  1:664264/1‑217 (MQ=255)                                                                                                                                                                                                              cGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGt                                          <  2:664264/217‑1 (MQ=255)                                                                                                                                                                                                                aaGGCGTAGTTCGCCTGACCTTTAATCTTGTCCGGAATCCCTACTACGGCGGCTTCGGCAATCGTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCaa                      <  2:44875/234‑1 (MQ=255)                                                                                                                                                                                                                        gATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt             <  1:488611/236‑1 (MQ=255)                                                                                                                                                                                                                        gATCGCCGGACCTTTAAGCTTGTGCGGAATACCTACCACGGCGGCTTCGGCAATCTTCGGATGCACCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt             <  1:352495/236‑1 (MQ=255)                                                                                                                                                                                                                         aTCGCCTTACCTTTAATCTTGTGCGGAATGCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt             <  1:752287/235‑1 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑Tcgccg                                                                 <  2:738407/179‑1 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑Tcgccg                                                                 >  1:738407/1‑179 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGa                                              >  1:107701/1‑198 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGa                                              <  2:107701/198‑1 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCg        >  1:298972/1‑236 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCg        >  1:764111/1‑236 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGGAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGAttg        >  1:596474/1‑234 (MQ=255)                                                                                                                                                                                                                             ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACCCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGCAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGAtat        >  1:244701/1‑234 (MQ=255)                                                                                                                                                                                                                                   cTTTAATCTTGTGCGGAATTCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAAc  <  2:152922/236‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       GTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAC‑GTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCG‑TCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAAC  >  NC_000913/4285480‑4285941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑