Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	650,553	T→G	A77A (GCA→GCC)	citD ←	citrate lyase, acyl carrier (gamma) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	650,553	0	T	G	100.0%	174.1 / NA	50	A77A (GCA→GCC)	citD	citrate lyase, acyl carrier (gamma) subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/33);  total (17/33)

GGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTT  >  NC_000913/650322‑650780                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     ggcgggcgGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCAc                                                                                                                                                                                                                                  <  1:519723/236‑1 (MQ=255)     ggcggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑a                                                                                                                                                                                                                              <  1:102461/235‑1 (MQ=255)                   cACGGATTGCTACGGAGTCTTCGATGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCCCCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTg                                                                                                                                                                                                               <  2:529840/236‑1 (MQ=255)                                ggAGTCGTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCAt                                                                                                                                                                                                   <  1:311062/235‑1 (MQ=255)                                     cTTCGAGGTCAACCTGCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACa                                                                                                                                                                                              <  2:757696/235‑1 (MQ=255)                                        ccacatCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCAACGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACa                                                                                                                                                                                              <  1:119109/227‑1 (MQ=255)                                                    tCAGGGCATACGCCGGGTAGATGAAGGAGTTGCTGACCATTGCGGCATTTGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGGAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgccgc                                                                                                                                                                              <  1:267311/236‑1 (MQ=255)                                                                         tGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCgaga                                                                                                                                                          <  1:257847/235‑1 (MQ=255)                                                                              gAGTTGCTAGCCATCGCGGAATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTCGGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTc                                                                                                                                                     <  2:437796/235‑1 (MQ=255)                                                                               aGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCa                                                                                                                                                   <  1:597876/236‑1 (MQ=255)                                                                                   gCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGATGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGaa                                                                                                                                                <  1:286689/235‑1 (MQ=255)                                                                                            cgcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTTTTGTAGGGAAGCGGGAAACATTGGCGATCCTCCCCTAGGAGAGGCGGGATACCGCCGGCGCGTGCCAGCCGGGGTTCCCACCGGGGCCGTGAAAATACAGGCCCGGGGGCCCTTGAC‑TCGACATTTACCAGGTCGCCGCGCACGCTGGGACGGGCGGGAAAGTCCAGaacaagggtgcg                                                                                                                                      >  2:552705/1‑226 (MQ=255)                                                                                                  aTTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTg                                                                                                                                 <  1:476086/235‑1 (MQ=255)                                                                                                                        aTGCTGCGGCTGTTGCGAGTTTTACGTTGTTGCAGCGAAGCGTAAATCATTGGCAATCCTCCCATGTCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACg                                                                                                          <  2:418005/236‑1 (MQ=255)                                                                                                                                         aGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAGCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCa                                                                                          <  1:734626/235‑1 (MQ=255)                                                                                                                                                   tgttgAAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATcc                                                                               <  1:363034/236‑1 (MQ=255)                                                                                                                                                    gttgCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCt                                                                              <  2:362412/236‑1 (MQ=255)                                                                                                                                                    gttgCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCt                                                                              <  1:405060/236‑1 (MQ=255)                                                                                                                                                     ttgCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTTTCGGGCGCGAACGTCCAGAATGGTGGTGCGAACTGCATCGCCTAACTGATTCCTAACGCTGCTATCGATTTGCAAGTCGATATCCTg                                                                             >  2:453751/1‑236 (MQ=255)                                                                                                                                                         aGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGt                                                                          <  1:670562/235‑1 (MQ=255)                                                                                                                                                         aGCGAAGCGGAAATCAGTTGCAATCCTCCCAGGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGGCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTa                                                                         <  2:51444/236‑1 (MQ=255)                                                                                                                                                           cGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACGGTTTCTCAACGCGGCTATTGATTTGCAGGTCGATATCCTGCGCATc                                                                       >  2:267814/1‑236 (MQ=255)                                                                                                                                                               gCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGt                                                                   >  2:765928/1‑236 (MQ=255)                                                                                                                                                               gCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGt                                                                   >  1:709189/1‑236 (MQ=255)                                                                                                                                                               gCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGGCGATATCCTGCGTATCGAGt                                                                   >  1:593020/1‑236 (MQ=255)                                                                                                                                                               gCGGAAATCATGGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAg                                                                    <  1:282979/235‑1 (MQ=255)                                                                                                                                                                    aaTCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTggg                                                                <  1:81597/234‑1 (MQ=255)                                                                                                                                                                             cAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGCATTGTGCGATGCGTa                                                      >  1:315367/1‑235 (MQ=255)                                                                                                                                                                             cAATCCTCCCATGGCAGAGCCGGGACTCCGCTGTCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTa                                                      <  2:593020/235‑1 (MQ=255)                                                                                                                                                                              aaTCCTCCCATGGCAGCGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGG‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTatc                                                    <  2:645315/236‑1 (MQ=255)                                                                                                                                                                                tcctccCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTatcat                                                  >  1:392326/1‑236 (MQ=255)                                                                                                                                                                                        gggCAGGGCCGGGATCCCGCTGGCGCGTTCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATccc                                          <  1:356604/235‑1 (MQ=255)                                                                                                                                                                                         ggCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATcccc                                         <  1:144543/236‑1 (MQ=255)                                                                                                                                                                                         ggCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAAGGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATcccc                                         <  2:594532/236‑1 (MQ=255)                                                                                                                                                                                          gCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCa                                                                                          >  1:520708/1‑186 (MQ=255)                                                                                                                                                                                          gCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCa                                                                                          <  2:520708/186‑1 (MQ=255)                                                                                                                                                                                           cAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGt                                                                          <  2:334486/201‑1 (MQ=255)                                                                                                                                                                                           cAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGt                                                                          >  1:334486/1‑201 (MQ=255)                                                                                                                                                                                           cAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATcaca                                              >  2:356604/1‑229 (MQ=255)                                                                                                                                                                                              agCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACt                                    >  1:168895/1‑236 (MQ=255)                                                                                                                                                                                              agCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGGTTCTCAACGCTGATATTGATTTGCAGGTCGATATCCTGCGTATCCACTGGGGCGATGGCTATCATCACATtccctggcc                                    >  2:371507/1‑231 (MQ=255)                                                                                                                                                                                               gCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTggg                                                                >  2:269817/1‑207 (MQ=255)                                                                                                                                                                                               gCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTggg                                                                <  1:269817/207‑1 (MQ=255)                                                                                                                                                                                                  gggATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGAc                                     <  1:552705/231‑1 (MQ=255)                                                                                                                                                                                                         cGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGCATGGGGCGCTGCGTATCATCACATCCCCGAACTCAACGGAGCCt                         >  2:397095/1‑236 (MQ=255)                                                                                                                                                                                                              gcgcGTGCCAGCAGGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAAc                    >  2:588609/1‑236 (MQ=255)                                                                                                                                                                                                                      cagcagGGCTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGc             <  1:588609/235‑1 (MQ=255)                                                                                                                                                                                                                              cTTCCAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCCCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCGCGTTTAtt    >  2:756749/1‑236 (MQ=255)                                                                                                                                                                                                                                 ccAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTAtttt  <  2:213481/235‑1 (MQ=255)                                                                                                                                                                                                                                 ccAGTCGGGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTAtttt  <  1:397095/235‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     GGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGT‑AAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGTTGTAGCGGGCGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTT  >  NC_000913/650322‑650780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑