Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,822,043	T→G	I72L (ATC→CTC)	osmE ←	osmotically‑inducible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,822,043	0	T	G	100.0%	191.1 / NA	54	I72L (ATC→CTC)	osmE	osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/23);  total (31/23)

CTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCC  >  NC_000913/1821811‑1822265                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGt                                                                                                                                                                                                                               >  2:733757/1‑235 (MQ=255)       aTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTg                                                                                                                                                                                                                        >  2:93866/1‑236 (MQ=255)            gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCaa                                                                                                                                                                                                                    >  1:705571/1‑235 (MQ=255)                  ataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACcg                                                                                                                                                                                                              <  1:400614/235‑1 (MQ=255)                       tCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCa                                                                                                                                                                                                        <  1:663089/236‑1 (MQ=255)                            aaGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGa                                                                                                                                                                                                    >  1:278511/1‑235 (MQ=255)                                   ttAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCt                                                                                                                                                                                             <  1:129940/235‑1 (MQ=255)                                         aCTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGaggtaggt                                                                                                                                                                                                                           >  1:614235/1‑199 (MQ=255)                                         aCTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGaggtaggt                                                                                                                                                                                                                           <  2:614235/199‑1 (MQ=255)                                         aCTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGc                                                                                                                                                                                                                      <  2:760530/204‑1 (MQ=255)                                         aCTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGc                                                                                                                                                                                                                      >  1:760530/1‑204 (MQ=255)                                         aCTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                                                                                                                                                      >  1:82012/1‑236 (MQ=255)                                             aGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACg                                                                                                                                                                                  >  1:730110/1‑236 (MQ=255)                                               gCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGc                                                                                                                                                                                                         >  1:8742/1‑211 (MQ=255)                                               gCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGc                                                                                                                                                                                                         <  2:8742/211‑1 (MQ=255)                                                         aCCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGCTTACCCg                                                                                                                                                                      >  1:175910/1‑236 (MQ=255)                                                                     gCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgtg                                                                                                                                                             >  2:96456/1‑233 (MQ=255)                                                                                   ttACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCt                                                                                                                                             >  1:410010/1‑235 (MQ=255)                                                                                      cTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATg                                                                                                                                         >  2:440329/1‑236 (MQ=255)                                                                                                           aTTACTTCGCTGCCTATGGATCAGGTGCGGAATCAGCACAGGTCTGAAAACCGGAGTGGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTcacc                                                                                                                    <  2:652503/236‑1 (MQ=255)                                                                                                           aTTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTcacc                                                                                                                    <  1:96456/236‑1 (MQ=255)                                                                                                               cTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAg                                                                                                                <  1:326394/236‑1 (MQ=255)                                                                                                                         tgtgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑a                                                                                                         >  1:161898/1‑232 (MQ=255)                                                                                                                           tgGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACt                                                                                                   <  1:440329/236‑1 (MQ=255)                                                                                                                                   tgtCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTggacgtt                                                                                            >  2:349916/1‑230 (MQ=255)                                                                                                                                     tCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGCCTTTGGt                                                                                         >  1:626012/1‑236 (MQ=255)                                                                                                                                       gTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGt                                                                                         <  1:93866/234‑1 (MQ=255)                                                                                                                                       gTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTa                                                                                        <  1:4249/235‑1 (MQ=255)                                                                                                                                       gTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTa                                                                                        <  2:82012/235‑1 (MQ=255)                                                                                                                                       gTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTa                                                                                        <  2:161898/235‑1 (MQ=255)                                                                                                                                             aGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCAt                                                                                 <  1:733757/236‑1 (MQ=255)                                                                                                                                               cacaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATaa                                                                               >  1:439032/1‑236 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                                                                                                                                                            <  1:294178/139‑1 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                                                                                                                                                            >  2:294178/1‑139 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCttt                                                                                                                        <  2:246381/191‑1 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCttt                                                                                                                        >  1:246381/1‑191 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTa                                                                                        <  1:461632/222‑1 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTa                                                                                        >  2:461632/1‑222 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCg                                                                           >  1:185142/1‑235 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGt                                                                          >  1:202594/1‑236 (MQ=255)                                                                                                                                                    gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGt                                                                          >  1:740013/1‑236 (MQ=255)                                                                                                                                                                 aGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGACTTTGGTACGATCATAAGCCGTACAACCCGCCAGc                                                             >  2:472108/1‑236 (MQ=255)                                                                                                                                                                       tgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGtt                                                       <  2:439032/236‑1 (MQ=255)                                                                                                                                                                           aTGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACg                                                                                                                                                                                  <  1:721309/110‑1 (MQ=255)                                                                                                                                                                           aTGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACg                                                                                                                                                                                  >  2:721309/1‑110 (MQ=255)                                                                                                                                                                            tGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGc                                                             <  1:410869/225‑1 (MQ=255)                                                                                                                                                                                   tATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTGTTTCACCACAGGCTGT‑ACAAACTCGTCTTTGGTACGATCATAAGCCGAACAACCTCCCAGccagatta                                                      >  2:410869/1‑218 (MQ=255)                                                                                                                                                                                       gtcTAACGCGAAAAAGTAGGTTTCTGCTTAACCTGCGCTTTGCCCCGGGAGTTAGGTCGGGCAATTACCGGGAGCGTGGATCTTGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCACCCTGCGCCCGGCTCATGCCTTTTTGGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACACCCCGCCAGCATGGTTAATACCGCCGCTGCa                                        <  2:740013/234‑1 (MQ=255)                                                                                                                                                                                        tcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACt                                      >  1:500206/1‑236 (MQ=255)                                                                                                                                                                                        tcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACt                                      >  1:337521/1‑236 (MQ=255)                                                                                                                                                                                                gACAAGGTAGTTTACTGCTTAACCAACACGTTCACCCAGGAGTTAGGTCGGGAAAGTCCCGGGATCATGAACCGTGCTCCCTCAAGACGAAGTTTTACCCACAATTTGGCCACCCTGCGCCCGCCCCTTGCTTTTTTGGCCGTCTTCCACCACGGCCTGA‑AAAACCTGGTTTTTGGTACGATCAAAAGCCGTACACCCCGCAAGCATGGTTAATACCGCCGCTGCACTCAGAATTc                              <  2:175910/236‑1 (MQ=255)                                                                                                                                                                                                                         tCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCATTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTACTGCCAAATTCTTATTCATAtttc      >  2:709477/1‑233 (MQ=255)                                                                                                                                                                                                                           aCGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTc       >  1:627816/1‑232 (MQ=255)                                                                                                                                                                                                                            cGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCCCTGCATTCAGAATTCCTGCCTAATTCTGTTTCTATTTCCGTcc  >  2:346488/1‑236 (MQ=255)                                                                                                                                                                                                                            cGTTCACCGAGGAGTTATGTCGGGAAAGTACCGCGACCAGGAATCATGCTCACTGCAGAGGAGGTTTGCCCCGCATTTGGTGACCCCGCCGCCGGGCTAAT‑CCTTTTTTGAACTTTTTCCCCCACGGCTTTAAAAAACGGGTTTTTGGTACGATAAAAACCCGTACACCCCCCCAGCATGGTAAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTcc  <  2:627816/236‑1 (MQ=255)                                                                                                                                                                                                                                gACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTcct           >  1:360837/1‑221 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                CTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGT‑ACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCC  >  NC_000913/1821811‑1822265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑