BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,361,316	Δ1 bp	coding (99/1206 nt)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,361,316	0	C	.	98.3%	820.7 / 9.5	178	coding (99/1206 nt)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base . (90/83); total (93/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

Only 100 of 182 total aligned reads displayed.

GTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGG  >  NC_000913/2361221‑2361410

 tGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑Gccccc                                                                                          <  1:654361/100‑1 (MQ=255)

  gCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCt                                                                                         <  1:5527014/100‑1 (MQ=255)

     aaGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGt                                                                                      >  1:2837342/1‑100 (MQ=255)

      aGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTc                                                                                     >  1:2799907/1‑100 (MQ=255)

       gAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCg                                                                                    <  1:2278977/100‑1 (MQ=255)

       gAACGGTAATGCCAAATGACTGGCGTCACTGCCCGTAATCGCGGCATCTACTCATCGGGGTGGCAATAGGACTGTCTATCCAGTG‑TTTGCCCCCtcgtcg                                                                                    >  2:309151/1‑100 (MQ=255)

        aaCGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGt                                                                                   <  2:3400344/100‑1 (MQ=255)

           gTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGAt                                                                                >  1:974013/1‑100 (MQ=255)

            ttAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATa                                                                               >  2:1545986/1‑100 (MQ=255)

            ttAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATa                                                                               <  2:1011117/100‑1 (MQ=255)

             tAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATa                                                                               >  1:3412728/1‑100 (MQ=255)

             tAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATaa                                                                              <  2:2834587/100‑1 (MQ=255)

              aaTGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAAt                                                                             <  1:1877583/100‑1 (MQ=255)

                   cAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCAccg                                                                        >  1:4298904/1‑100 (MQ=255)

                    aaaTGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCAccgc                                                                       >  1:2899465/1‑100 (MQ=255)

                     aaTGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCAccgcc                                                                      >  2:3576091/1‑100 (MQ=255)

                      aTGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCAccgccg                                                                     <  1:4353556/100‑1 (MQ=255)

                        gACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCgccgcc                                                                   <  2:1563539/100‑1 (MQ=255)

                        gACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCgccgcc                                                                   >  1:4476676/1‑100 (MQ=255)

                        gACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCgccgcc                                                                   <  2:455555/100‑1 (MQ=255)

                         aCTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCa                                                                  >  1:4171871/1‑100 (MQ=255)

                          cTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAg                                                                 >  1:4041157/1‑100 (MQ=255)

                               gTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCt                                                            >  2:2614942/1‑100 (MQ=255)

                                 gACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCttt                                                          >  1:1049284/1‑100 (MQ=255)

                                  aCTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCtttt                                                         <  2:4936635/100‑1 (MQ=255)

                                   cTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCttttt                                                        >  1:1460857/1‑100 (MQ=255)

                                   cTGCTCGTAATCGCGGTATTTACTCATCGGGGTGACAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCttttt                                                        <  2:2261241/100‑1 (MQ=255)

                                    tGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAGTATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc                                                       <  1:5253571/100‑1 (MQ=255)

                                    tGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCttttcc                                                       <  2:5090129/100‑3 (MQ=255)

                                    tGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc                                                       >  1:5367374/1‑100 (MQ=255)

                                    tGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc                                                       <  1:571889/100‑1 (MQ=255)

                                    tGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc                                                       <  1:4242863/100‑1 (MQ=255)

                                    tGCTCGTAATCGCGGCATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc                                                       <  2:2795672/100‑1 (MQ=255)

                                     gCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTct                                                      >  2:3956745/1‑100 (MQ=255)

                                      ctctTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctg                                                     >  2:5040458/1‑100 (MQ=255)

                                      cgcGTACTTGCGGTATTTACACCGCGCGGTGGTGATCTGATAGTCTATCCAGCGGTT‑GCCTCCTGGTCGTGATAATCACGGCCGCCCGCGCCTTTTTctg                                                     <  2:4334634/98‑1 (MQ=255)

                                      cTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctg                                                     <  2:3652801/100‑1 (MQ=255)

                                      cTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctg                                                     >  1:2636771/1‑100 (MQ=255)

                                      cTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctg                                                     <  1:2640658/100‑1 (MQ=255)

                                        cGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgct                                                   >  1:3191111/1‑100 (MQ=255)

                                         gTAAGCGCGGTATTTACTCATCTTGACGGACACATCATCGTGTGTCCAGTGATT‑GCATCCTGCTAGTGATAATCAGCGCCCACCCCGCCTTTTTctgacg                                                  >  2:4657895/1‑97 (MQ=255)

                                          tAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGt                                                 >  2:715771/1‑100 (MQ=255)

                                            aTCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCg                                               >  2:2956047/1‑100 (MQ=255)

                                              cgcgGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCa                                             >  2:5400066/1‑100 (MQ=255)

                                               gcgGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                            >  2:2656544/1‑100 (MQ=255)

                                               gcgGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                            >  1:3842095/1‑100 (MQ=255)

                                                cgGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAcc                                           <  2:852273/100‑1 (MQ=255)

                                                   tATTTAGTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GTCCCCCGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGGACCGcc                                        <  2:5173389/100‑1 (MQ=255)

                                                   tATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGcc                                        <  1:1026485/100‑1 (MQ=255)

                                                   tATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGcc                                        <  1:2408810/100‑1 (MQ=255)

                                                   tATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGcc                                        <  1:3727474/100‑1 (MQ=255)

                                                     tttACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAg                                      <  2:2679543/100‑1 (MQ=255)

                                                      ttACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGt                                     >  2:3240396/1‑100 (MQ=255)

                                                       tACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTa                                    >  2:2078465/1‑100 (MQ=255)

                                                          tCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAc                                 >  1:810354/1‑100 (MQ=255)

                                                          tCATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAc                                 <  2:51966/100‑1 (MQ=255)

                                                           cATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAcc                                >  2:2508762/1‑100 (MQ=255)

                                                           cATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAcc                                >  1:3610598/1‑100 (MQ=255)

                                                           cATCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAcc                                <  2:3020521/100‑1 (MQ=255)

                                                            aTCGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCa                               >  1:1264307/1‑100 (MQ=255)

                                                              cGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAgg                             >  1:1676367/1‑100 (MQ=255)

                                                              cGGGGTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAgg                             >  1:3365665/1‑100 (MQ=255)

                                                                gggTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcg                           >  2:2904344/1‑100 (MQ=255)

                                                                  gTGGCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGcgcg                         <  2:776567/100‑1 (MQ=255)

                                                                     gCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAc                      <  2:2734427/100‑1 (MQ=255)

                                                                     gCAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCC‑TTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAcc                     <  1:3572815/100‑1 (MQ=255)

                                                                      cAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAcc                     <  2:49602/100‑1 (MQ=255)

                                                                      cAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAcc                     >  2:1744587/1‑100 (MQ=255)

                                                                      cAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAcc                     <  2:3342210/100‑1 (MQ=255)

                                                                      cAATATGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTCCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAcc                     >  1:4195361/1‑100 (MQ=255)

                                                                        atatGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCt                    >  2:3852926/1‑100 (MQ=255)

                                                                           tGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                <  1:3821214/100‑1 (MQ=255)

                                                                           tGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                >  1:1398107/1‑100 (MQ=255)

                                                                           tGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                >  1:1442377/1‑100 (MQ=255)

                                                                           tGATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                <  1:2970743/100‑1 (MQ=255)

                                                                            gATCGTCTGTCCAGTGA‑TGGCCCCCTGGTCGTTATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCTAGTAAACCAGGCGCGAATCTGTTTa               <  2:1008670/100‑1 (MQ=255)

                                                                            gATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTa               >  1:1357099/1‑100 (MQ=255)

                                                                            gATCGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTa               >  2:1449111/1‑100 (MQ=255)

                                                                               cGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAtt            <  1:1312085/100‑1 (MQ=255)

                                                                               cGTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAtt            >  1:4796919/1‑100 (MQ=255)

                                                                                gTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAttt           >  1:5028063/1‑100 (MQ=255)

                                                                                gTCTATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAttt           >  1:3075048/1‑100 (MQ=255)

                                                                                   tATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg        <  2:3302936/100‑1 (MQ=255)

                                                                                   tATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg        <  1:3806494/100‑1 (MQ=255)

                                                                                   tATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg        <  1:2005348/100‑1 (MQ=255)

                                                                                   tATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg        <  1:1797845/100‑1 (MQ=255)

                                                                                   tATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg        <  2:1317777/100‑1 (MQ=255)

                                                                                   tATCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg        <  1:3469464/100‑1 (MQ=255)

                                                                                   tATCCAGTGATT‑GCCCCCTGGTCGTGAAAATCACCGCCGCCAGCGCCTTTTTCTCCTGTTGCACCGCCATTAAACCAGGCGCGACCCTGTTTAATTTTTg        <  1:4839193/100‑1 (MQ=255)

                                                                                    aTCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTgg       <  1:1092354/100‑1 (MQ=255)

                                                                                    aTCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTgg       >  2:4406119/1‑100 (MQ=255)

                                                                                     tCCAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGt      >  2:1107960/1‑100 (MQ=255)

                                                                                      ccAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTa     <  2:375022/100‑1 (MQ=255)

                                                                                      ccAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTa     >  1:2966717/1‑100 (MQ=255)

                                                                                       cAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAg    >  2:1229389/1‑100 (MQ=255)

                                                                                       cAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAg    >  1:4598087/1‑100 (MQ=255)

                                                                                       cAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAg    >  1:246243/1‑100 (MQ=255)

                                                                                       cAGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAg    <  1:3275173/100‑1 (MQ=255)

                                                                                        aGTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAgg   <  1:2833168/100‑1 (MQ=255)

                                                                                         gTGATT‑GCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAggg  <  1:4912807/100‑1 (MQ=255)

GTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGG  >  NC_000913/2361221‑2361410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`