Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,861	(T)8→7	intergenic (‑101/‑45)	dapA ← / → gcvR	dihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,854	0	T	.	100.0%	210.1 / NA	45	intergenic (‑94/‑52)	dapA/gcvR	dihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/23);  total (22/23)

GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAG  >  NC_000913/2599704‑2599997                                                                                                                                                       |                                                                                                                                                gacaTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGt                                                                                                                                                 >  2:374501/1‑151 (MQ=255)  acaTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGtt                                                                                                                                                >  2:215915/1‑151 (MQ=255)                       ggAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGAGTCTCATTGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGGTTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaa                                                                                                                          >  2:225332/1‑151 (MQ=255)                        gAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaa                                                                                                                          >  2:469936/1‑150 (MQ=255)                        gAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaa                                                                                                                          <  1:83292/150‑1 (MQ=255)                           tAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaacaac                                                                                                                      <  2:579525/151‑1 (MQ=255)                                    cgACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCtt                                                                                                             >  2:633200/1‑151 (MQ=255)                                       cAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTaaa                                                                                                          >  1:641471/1‑151 (MQ=255)                                       cAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTaaa                                                                                                          <  2:27510/151‑1 (MQ=255)                                        aaTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAg                                                                                                         <  1:65038/151‑1 (MQ=255)                                        aaTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAg                                                                                                         >  1:40123/1‑151 (MQ=255)                                         aTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAgg                                                                                                        <  2:446231/151‑1 (MQ=255)                                         aTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAgg                                                                                                        <  1:470652/151‑1 (MQ=255)                                         aTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAgg                                                                                                        <  2:9399/151‑1 (MQ=255)                                         aTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAgg                                                                                                        <  2:335679/151‑1 (MQ=255)                                          tACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGa                                                                                                       >  1:219234/1‑151 (MQ=255)                                                    gAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGtt                                                                                             >  1:438393/1‑151 (MQ=255)                                                    gAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGtt                                                                                             >  1:147408/1‑151 (MQ=255)                                                          gggCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGaca                                                                                        >  1:309926/1‑150 (MQ=255)                                                           ggCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGacac                                                                                       <  2:464787/150‑1 (MQ=255)                                                                    ctGTGCAAACAAGTTTCTCAATATTACGTTTGGTATGTCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTGTTTATGCTTTCCTTAAGAACAGCTCACCCCTTAAAGGAATAGCCAGTTTGACACTGTCATctct                                                                             <  2:79931/151‑4 (MQ=255)                                                                         cAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACAt                                                                         <  2:220147/150‑1 (MQ=255)                                                                             cAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTAt                                                                      >  2:109237/1‑149 (MQ=255)                                                                             cAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCt                                                                    <  2:591247/151‑1 (MQ=255)                                                                                   tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGa                                                               <  2:8357/150‑1 (MQ=255)                                                                                   tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGAt                                                              >  1:141017/1‑151 (MQ=255)                                                                                   tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGAt                                                              >  2:264543/1‑151 (MQ=255)                                                                                   tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGAt                                                              >  2:338749/1‑151 (MQ=255)                                                                                   tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGAt                                                              >  1:376128/1‑151 (MQ=255)                                                                                        aTGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCa                                                            >  1:301419/1‑148 (MQ=255)                                                                                          ggTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCg                                                       >  1:597420/1‑151 (MQ=255)                                                                                            tACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGtt                                                     <  1:490480/151‑1 (MQ=255)                                                                                               gTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTggg                                                  >  1:346709/1‑151 (MQ=255)                                                                                                  tGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGc                                               <  2:493188/151‑1 (MQ=255)                                                                                                     tATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGa                                            <  1:137948/151‑1 (MQ=255)                                                                                                           aTTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGcc                                       >  2:449952/1‑150 (MQ=255)                                                                                                              aaaaGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTgg                                   >  1:99896/1‑151 (MQ=255)                                                                                                                   caagcaGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAAt                                <  2:4885/149‑1 (MQ=255)                                                                                                                   caagcaGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAAt                                <  2:499547/149‑1 (MQ=255)                                                                                                                   caagcaGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATtg                              <  2:157701/151‑1 (MQ=255)                                                                                                                        agacagaACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGaa                          >  1:98300/1‑150 (MQ=255)                                                                                                                              aaCCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAacac                       <  2:99893/147‑1 (MQ=255)                                                                                                                                  gTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCAcc                >  2:566685/1‑150 (MQ=255)                                                                                                                                   ttCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGaa                                 >  2:167542/1‑132 (MQ=255)                                                                                                                                   ttCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGaa                                 <  1:167545/132‑1 (MQ=255)                                                                                                                                        aTTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATg         <  1:53826/151‑1 (MQ=255)                                                                                                                                               tATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagtag  <  1:590464/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAG  >  NC_000913/2599704‑2599997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑