Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,861	(T)8→7	intergenic (‑101/‑45)	dapA ← / → gcvR	dihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,854	0	T	.	100.0%	399.1 / NA	82	intergenic (‑94/‑52)	dapA/gcvR	dihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (40/42);  total (40/42)

GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTT  >  NC_000913/2599704‑2599999                                                                                                                                                       |                                                                                                                                                  gacaTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGt                                                                                                                                                   <  2:380056/151‑1 (MQ=255) gacaTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGt                                                                                                                                                   <  2:905301/151‑1 (MQ=255)  acaTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGtt                                                                                                                                                  <  1:46536/151‑1 (MQ=255)   caTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGttt                                                                                                                                                 >  2:1265772/1‑151 (MQ=255)     ttACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGttttt                                                                                                                                               >  2:107189/1‑151 (MQ=255)     ttACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGttttt                                                                                                                                               >  1:194190/1‑151 (MQ=255)      tACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGtttttt                                                                                                                                              >  2:754654/1‑151 (MQ=255)       aCCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGttttttt                                                                                                                                             >  2:195970/1‑151 (MQ=255)        ccTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGttttttta                                                                                                                                            <  1:1323339/151‑2 (MQ=255)            tttCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGtttttttatgc                                                                                                                                         <  2:21880/150‑5 (MQ=255)            tttCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCt                                                                                                                                       >  1:342125/1‑151 (MQ=255)            tttCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCt                                                                                                                                       >  1:1305530/1‑151 (MQ=255)              tCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCtt                                                                                                                                      <  2:445275/150‑1 (MQ=255)              tCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCtt                                                                                                                                      <  1:1141333/150‑1 (MQ=255)                 tccatcGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCt                                                                                                                                  >  2:1087210/1‑151 (MQ=255)                  ccatcGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCtt                                                                                                                                 <  2:691475/151‑1 (MQ=255)                  ccatcGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCtt                                                                                                                                 <  2:946963/151‑1 (MQ=255)                      cGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGa                                                                                                                             <  1:920858/151‑1 (MQ=255)                         aGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaac                                                                                                                           >  1:1196638/1‑150 (MQ=255)                          gTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaacaa                                                                                                                         <  2:1035557/151‑1 (MQ=255)                          gTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaaca                                                                                                                          >  2:983003/1‑150 (MQ=255)                            aaCAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACt                                                                                                                       <  2:575012/151‑1 (MQ=255)                            aaCAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACt                                                                                                                       >  2:260528/1‑151 (MQ=255)                              cAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTc                                                                                                                      >  2:1092384/1‑150 (MQ=255)                              cAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTc                                                                                                                      >  1:531769/1‑150 (MQ=255)                                   gcgACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCt                                                                                                                >  2:505490/1‑151 (MQ=255)                                       cAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTaaa                                                                                                            <  1:478452/151‑1 (MQ=255)                                        aaTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTaa                                                                                                             >  2:60458/1‑149 (MQ=255)                                         aTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAgg                                                                                                          >  1:130592/1‑151 (MQ=255)                                              tCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATa                                                                                                      <  2:260665/150‑1 (MQ=255)                                               cccGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATaa                                                                                                     <  1:1237962/150‑1 (MQ=255)                                                 cGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAAcc                                                                                                   >  1:1221914/1‑150 (MQ=255)                                                 cGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAAcc                                                                                                   >  1:1129599/1‑150 (MQ=255)                                                 cGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑‑TTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAg                                                                                                 <  1:1205662/151‑1 (MQ=255)                                                     aaCATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGttt                                                                                              >  1:1138987/1‑151 (MQ=255)                                                      aCATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTg                                                                                             <  2:156677/151‑1 (MQ=255)                                                      aCATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTg                                                                                             >  2:558581/1‑151 (MQ=255)                                                        aTGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGac                                                                                           <  2:1095989/151‑1 (MQ=255)                                                         tGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGac                                                                                           >  2:1280439/1‑150 (MQ=255)                                                           ggCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACt                                                                                        <  1:794781/151‑1 (MQ=255)                                                               aTCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCa                                                                                    <  2:1305041/151‑1 (MQ=255)                                                               aTCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCa                                                                                    <  1:1026814/151‑1 (MQ=255)                                                                 ccTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCAt                                                                                   <  2:964830/150‑1 (MQ=255)                                                                 ccTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATc                                                                                  <  1:188194/151‑1 (MQ=255)                                                                  ctctGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCg                                                                                 >  2:999836/1‑151 (MQ=255)                                                                      gtgCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCaa                                                                              <  1:1129889/150‑1 (MQ=255)                                                                        gCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACAt                                                                           >  1:603302/1‑151 (MQ=255)                                                                         cAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACAtt                                                                          >  1:96146/1‑151 (MQ=255)                                                                          aaaCAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTa                                                                         <  2:247053/151‑1 (MQ=255)                                                                            aCAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATc                                                                       <  2:1101121/151‑1 (MQ=255)                                                                                    ctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATc                                                               >  2:1103731/1‑151 (MQ=255)                                                                                      cAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATc                                                               <  1:540979/149‑1 (MQ=255)                                                                                      cAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCa                                                              <  2:565134/150‑1 (MQ=255)                                                                                          ggTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGc                                                          <  1:711073/150‑1 (MQ=255)                                                                                          ggTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCg                                                         <  1:150501/151‑1 (MQ=255)                                                                                             aCGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTg                                                      <  2:269688/151‑1 (MQ=255)                                                                                               gTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTggg                                                    >  1:834649/1‑151 (MQ=255)                                                                                                tttGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGt                                                   >  2:1207195/1‑151 (MQ=255)                                                                                                 ttGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTg                                                  >  2:565672/1‑151 (MQ=255)                                                                                                      aTGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGa                                              <  1:1368750/150‑1 (MQ=255)                                                                                                      aTGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGAt                                             <  1:499692/151‑1 (MQ=255)                                                                                                        ggCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATc                                            <  1:195980/150‑1 (MQ=255)                                                                                                        ggCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATc                                            <  2:917201/150‑1 (MQ=255)                                                                                                         gCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGc                                          >  1:731198/1‑151 (MQ=255)                                                                                                          cATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGcc                                         >  1:1252018/1‑151 (MQ=255)                                                                                                             tAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCt                                       <  2:242408/150‑1 (MQ=255)                                                                                                              aaaaGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTgg                                     >  1:352504/1‑151 (MQ=255)                                                                                                              aaaaGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTgg                                     >  1:985620/1‑151 (MQ=255)                                                                                                                 agcaagcaGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAAt                                  <  1:244123/151‑1 (MQ=255)                                                                                                                   caagcaGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATtg                                >  1:282066/1‑151 (MQ=255)                                                                                                                   caagcaGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATtg                                >  1:739294/1‑151 (MQ=255)                                                                                                                     ggcaGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATtgtg                              <  2:281814/150‑1 (MQ=255)                                                                                                                        agacagaACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAac                           <  1:137834/151‑1 (MQ=255)                                                                                                                        agacagaACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAac                           >  2:322143/1‑151 (MQ=255)                                                                                                                            agaACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACAcc                        >  2:434566/1‑150 (MQ=255)                                                                                                                              aaCCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATc                     >  1:1138090/1‑151 (MQ=255)                                                                                                                                ccGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCAc                   >  1:1211997/1‑151 (MQ=255)                                                                                                                                  gTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCAccc                 >  1:865628/1‑151 (MQ=255)                                                                                                                                   ttCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCg                >  2:465345/1‑151 (MQ=255)                                                                                                                                       gATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTc              <  2:850996/149‑1 (MQ=255)                                                                                                                                       gATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTc              <  2:194180/149‑1 (MQ=255)                                                                                                                                       gATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCAt            >  2:775796/1‑151 (MQ=255)                                                                                                                                         ttgttgTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGt          >  1:1363879/1‑151 (MQ=255)                                                                                                                                          tgttgtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGt          <  1:327166/150‑1 (MQ=255)                                                                                                                                          tgttgtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGt          <  2:1190382/150‑1 (MQ=255)                                                                                                                                          tgttgtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTc         >  1:1285870/1‑151 (MQ=255)                                                                                                                                          tgttgtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTc         >  2:842851/1‑151 (MQ=255)                                                                                                                                             tgtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCag       <  2:138760/150‑1 (MQ=255)                                                                                                                                              gtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagta     <  2:624515/151‑1 (MQ=255)                                                                                                                                                aTGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAATACCATCACCCGTCATGTCagtag    <  2:351107/150‑1 (MQ=255)                                                                                                                                                aTGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagtag    <  1:555024/150‑1 (MQ=255)                                                                                                                                                 tGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGtt  >  2:886839/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                  GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTT  >  NC_000913/2599704‑2599999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑