Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	48.8%	109.4 / 25.3	80	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (9/32);  new base G (39/0);  total (48/32)
Fisher's exact test for biased strand distribution p-value = 1.67e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAA  >  NC_000913/1097436‑1097731                                                                                                                                                   |                                                                                                                                                         cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCg                                                                                                                                                      <  1:22098/150‑1 (MQ=31) cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCg                                                                                                                                                      <  1:1107317/150‑1 (MQ=31)     gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAAccc                                                                                                                                                 <  2:728140/151‑1 (MQ=34)       gTTCCCGGGCTGTCCAACTGGCTGTCGCCCGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAAccccc                                                                                                                                               <  2:726900/151‑1 (MQ=255)           aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATa                                                                                                                                           <  1:1001373/151‑1 (MQ=39)             ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAccccccgaacg                                                                                                                                         >  1:886761/1‑145 (MQ=255)             ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACg                                                                                                                                         <  1:528369/151‑1 (MQ=40)                  gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCACACGTTGcc                                                                                                                                    >  1:1021864/1‑151 (MQ=255)                  gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGtt                                                                                                                                       <  1:945964/148‑1 (MQ=255)                     catcTGGCTTTCTCCAGTTGTCGAACCCCGGTCGGTGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGta                                                                                                                                 <  2:420358/148‑1 (MQ=255)                     cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCCATACGTTGCCGt                                                                                                                                  >  1:1149028/1‑150 (MQ=255)                           gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCCATACGTTTCCCTATaaac                                                                                                                            >  1:1059373/1‑150 (MQ=11)                                   cAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCCCTACGTTGCCGTATACAC‑AC‑ctttc                                                                                                                   >  1:1264697/1‑147 (MQ=14)                                   cAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAg                                                                                                                 <  1:1097316/151‑1 (MQ=255)                                    aGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAgg                                                                                                                <  1:430746/151‑1 (MQ=255)                                        gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTg                                                                                                            <  1:882266/151‑1 (MQ=255)                                               cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACGCTGCCGGTTACAC‑AC‑TTTCCCGGCGGGCTCCTTc                                                                                                     >  2:515/1‑151 (MQ=17)                                               cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAAACCCCCATACGTTTCCGTATACAC‑AC‑TTTCCCGGCGTGCTCCTTc                                                                                                     >  1:144737/1‑151 (MQ=17)                                                ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCTCAACCCCCCTAACTTTCCGTATTCACAAC‑TTTCC‑GGCGTGCTCCTTcc                                                                                                    >  2:249339/1‑150 (MQ=11)                                                ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCa                                                                                                    <  2:811286/151‑1 (MQ=255)                                                 cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCCCACCTTTCCGTATACAA‑AC‑CTTTCCGGCGTGGTCCtttcc                                                                                                   >  2:146445/1‑148 (MQ=14)                                                       gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTc                                                                                             <  1:1341194/151‑1 (MQ=255)                                                         gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTAAGTTTCCGCATACAC‑AC‑TTTCCAGGCGGGGCCCTTCCGCCACCCgg                                                                                           >  2:1060000/1‑151 (MQ=14)                                                            tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCATACCTTGCCCTTTAAAA‑AC‑TTTCCAGGCGGGCCCCTTCAGCCCCCCgggc                                                                                         >  1:422369/1‑148 (MQ=17)                                                               cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCATACGTTTCCGTATAAAC‑AC‑CTTTCACGCGTGCTCCTTCAGcccccccggcaccc                                                                                     >  1:1317931/1‑139 (MQ=17)                                                               cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCt                                                                                     <  1:216877/151‑1 (MQ=255)                                                                aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTACGTTGCCGGATACAC‑AC‑CTTCCCGGCGGGCTCCTTCCGCCACTCCGGCACCTc                                                                                    >  1:192649/1‑151 (MQ=17)                                                                      ccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCCCACCTTGTCGTATAAAA‑AA‑TTTTCAGGGGTGGTCCCTCCGCCACCCGGGCACCCCACCaa                                                                               >  2:1307160/1‑150 (MQ=17)                                                                      ccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGTTTGAAACCCCCATACGTTTCCGTATAAAC‑AC‑CTTTCCGGCGGGCTCCCTCAGCCCCTCCGACACCCCCCCaa                                                                               >  1:510304/1‑150 (MQ=17)                                                                        ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAtt                                                                            <  1:981551/151‑1 (MQ=255)                                                                         gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCGCTACGTTGCCGTATACAC‑AC‑TTTCCCTGCGTGCTCCTTCCGCCACCCGGACACCTCACCAAAttt                                                                           >  1:222888/1‑150 (MQ=14)                                                                          tgtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACGTTGCCGTATAAAC‑AC‑TTTTCAGGCGGGCTCCCTCAGCCACCCCGGCACCCCCCCaaaattt                                                                          >  2:385680/1‑147 (MQ=14)                                                                          tgtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCCATACGTTGCCGTATACAA‑AC‑TTTTCACGCGTGCTCCTTCAGCCCCTCCGACACCTCACCaaaattt                                                                          >  1:994523/1‑147 (MQ=14)                                                                            tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACGTTGCCGTATAAAC‑AC‑TTTCCAGGCGTGCTCCCTCAGCCACTCGGACACCTCCCCAAATTTTCg                                                                        >  1:945566/1‑151 (MQ=14)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAAACCCCCCTACGTTTCCGTATACAC‑AC‑TTTTCCGGCGGGCCCCCTCAGCCACCCGGACACCCCCCCCAAATTTCGt                                                                       >  1:1116572/1‑151 (MQ=14)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGt                                                                       <  2:1207595/151‑1 (MQ=255)                                                                                cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCGATACGTTGCCGGATACAC‑AC‑CTTTCAGGCGGGCTCCTTCAGCCACTCGGGCACCTCACCAAAATTTCGTTcc                                                                    >  1:144746/1‑151 (MQ=14)                                                                                cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                                                                    <  2:984737/151‑1 (MQ=255)                                                                                      aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCCATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACCCCGACACCCCACCAAATTTTCGTTCCTTTCtt                                                              >  1:590709/1‑151 (MQ=14)                                                                                         aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACGTTGCCGTATACAC‑AC‑TTTTCAGGCGGGCTCCTTTCGCCACTCGGACACCTCACCAAAATTTCGTTCCTGTCTTTCt                                                           >  1:1226168/1‑151 (MQ=14)                                                                                         aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCCGGCGTGCTCCTTCAGCCACTCGGACACCCCACCAAATTGTCGTTCCTGTCTTGCt                                                           >  1:873110/1‑151 (MQ=255)                                                                                             aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCTAACCCCCCATCCCTTTACGTATTAAC‑AC‑TTTTCTGGCGTGCTCCTTCCGCCCCCCCGGCACCCCACCCCATTTTCGTTCCtttttttcttga                                                        >  2:470217/1‑139 (MQ=2)                                                                                              aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCATACGTTTCCGGATACAC‑AC‑TTTTCAGGCGGGCTCCTTCCGCCACCCCGGCACCCCACCAAATTTTCGTTTCTTTCTTTCTggga                                                       >  1:544983/1‑148 (MQ=14)                                                                                               aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                     <  1:670496/151‑1 (MQ=255)                                                                                                  cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgc                                                  <  1:808839/151‑1 (MQ=255)                                                                                                    cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTCGAACCCCCCCTACGTTTGCGGTTAAAC‑AC‑CTTCCACGGGTGGGCCTTCCGCCCCCCCGGCACCCCCCCCAAATTTCGGTCCTCTCTTTCTTGAAAgggggg                                                >  2:128726/1‑148 (MQ=2)                                                                                                    cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCATACGTTGCCGGATACAC‑AC‑TTTTCCCGCGGGCTCCTTCAGCCCCCCCGACACCCCCCCCAATTTTTGTTTCCGTCTTTCTGGAAAGGgcgc                                                >  1:1323601/1‑151 (MQ=9)                                                                                                     gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCt                                               <  1:600629/151‑1 (MQ=255)                                                                                                     gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCt                                               <  2:1148797/151‑1 (MQ=255)                                                                                                       aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTACGTTTTCCTATACAC‑AA‑TTTTCAGGCGGGGGCCTTCACCCACCCGGACACCCCCCCAAAATTTCGTTCCTGTCTTTCTGGAACGGgcgcca                                              >  2:970866/1‑148 (MQ=9)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAACCCCCCATAAGTTTCCGTATAAAA‑AC‑TTTTCAGGCGGGCTCCTTCAGCCACTCCGACACCCCACCCAAATTTCGTTCCTGTCTTTCTGGAACGGGCGCTAAttt                                          >  1:70079/1‑151 (MQ=17)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAtt                                           <  1:685656/150‑1 (MQ=255)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAt                                            <  2:888424/149‑1 (MQ=255)                                                                                                            cGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTACGTTTCCCGATACAC‑ACATTTCCA‑GCGGGCTCCTTCAGCCACCCCGACACCCCACCAAAATGTCGTTTCTTTCTTTCTGGAAAGGGCGCTCAtttt                                         >  1:671338/1‑149 (MQ=9)                                                                                                             gTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCGATACGTTTCCGGATACAA‑AC‑TTTTCAGGCGTGGTCCCTCAGCCCCTCGGAAACCTCCCCAAAATGTCGTTTCCGTTTTGCTGGGACGGGGGCGAATTTTgg                                       >  1:1078933/1‑151 (MQ=14)                                                                                                               aCGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTCGAACCCCCCATACGTTTCCGGTTTAAC‑AC‑TTTTCAGGGGGGGTCCTTCAGCCACCCGGACACCTCCCCCAAATTTTGTTTCTGTCTTTCTGGAACGGGCGCCAATTTTgggg                                     >  2:796602/1‑150 (MQ=9)                                                                                                               aCGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAggg                                      <  2:9898/150‑1 (MQ=255)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGGACCCCCCCTACGTTTCCGTATACAC‑AC‑TTTTCAGGCGTGGTCCTTCAGCCCCCCCGGCACCCCCCCCAAATTTCGTTCCTGTCTTTCTGGAACGGGCGCTCAtttttggga                                    >  2:755001/1‑144 (MQ=9)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTTGACCCCCCATACGTTGCCGTATACAC‑AC‑TTTCCAGGGGTGGTCCTTCAGCCACCCGGGCCCCCCACCAAAATGTCGTTCCTGTCTTTCTTGGACGGGCGCTAATTTTGGGaa                                    >  2:805131/1‑150 (MQ=17)                                                                                                                    ctctTCTTTAAATATGGCGGGGG‑GGGGGGGGGTTGAACCCCCCCTACGTTTCCGTATACAA‑AC‑TTTCCAGGCGGGGTCCTTCCGCCACCCGGGCACCCCCCCAAATTGTTGTTTCTGTCTTTCTGGGACGGGCGCCCATTTTGGGgaaaa                                 >  2:1056588/1‑148 (MQ=9)                                                                                                                     tcttctTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTTCTGGAACGGGCGCTAATTTAGGGAAATCAt                               >  1:156835/1‑151 (MQ=255)                                                                                                                      cttcttTAAATATGGCGGTGA‑GGGGGGGGTTCTAAACCCCCCTACGTTTCCGTATACAC‑AC‑CTTTCCGGGGGGCTCCCTCCGCCCCCCGGGCACCCCACCAAAATTTCGTTCCTGTCTTGCTGGGACGGGCGCCAAtttttgggaaacatt                              >  2:666522/1‑139 (MQ=12)                                                                                                                       ttcttTAAATATGGCGGTGAGGGGGGGGGTTGGAACCCCC‑ATACGTTGCCCGATACAC‑AC‑CTTTCAGGCGTGGCCCTTCCGCCACTCCGACACCCCACCAAATTTTCGTTTCTGTCTTTCTGGAACGGGCGCTAATTTTGGGGAAACATg                              >  1:428941/1‑150 (MQ=12)                                                                                                                          tttAAATATGGCGGTGA‑GGGGGGGGTTTGTAACCCCCCTACGTTTGCGTATACAC‑AC‑TTTCCCGGCGGGCGTCTTCAGCCCCTCGGACACCCCACCCAAATTTTGTTTCTGTCTTTTTGGGAAGGGCGCCCATTTAGGGGAAACATGAACt                          >  2:501953/1‑151 (MQ=9)                                                                                                                              aaTATGGCGGTGA‑GGGGGGGGTTCGGACCCCCCCAACGTTTGCGTATACAC‑CC‑TTTTCAGGCGGGGGCCTTCCGCCCCCCGGACACCTCCCCAAAATTTTGGTCCTGTCTTTCTGGAACGGGCGCCCATTTAGGGAAAACATGACCTGAgg                      >  2:419621/1‑151 (MQ=12)                                                                                                                               atatGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGt                     >  1:1277826/1‑151 (MQ=255)                                                                                                                               atatGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGt                     <  2:623501/151‑1 (MQ=255)                                                                                                                                 atGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAgggc                    >  1:1012738/1‑148 (MQ=255)                                                                                                                                   ggCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                 <  2:287022/151‑1 (MQ=255)                                                                                                                                   ggCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                 >  1:662307/1‑151 (MQ=255)                                                                                                                                      ggTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACaaa              <  1:1039183/151‑1 (MQ=255)                                                                                                                                        tGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACt            >  1:223337/1‑151 (MQ=255)                                                                                                                                        tGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACt            >  2:381753/1‑151 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt           <  1:1113160/151‑1 (MQ=255)                                                                                                                                            gggggggATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt         <  1:131766/151‑1 (MQ=255)                                                                                                                                            gggggggATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt         >  1:691560/1‑151 (MQ=255)                                                                                                                                              gggggATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg       <  2:1021671/151‑1 (MQ=255)                                                                                                                                               ggggATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg       >  1:832301/1‑150 (MQ=255)                                                                                                                                                gggATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaa     <  2:286339/151‑1 (MQ=255)                                                                                                                                                  gATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa   <  2:582344/151‑1 (MQ=255)                                                                                                                                                   aTTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaaa  <  1:536338/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                         CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑AC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAA  >  NC_000913/1097436‑1097731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑