Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	50.0%	82.7 / 69.2	92	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/41);  new base T (46/0);  total (51/41)
Fisher's exact test for biased strand distribution p-value = 1.14e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206285‑3206583                                                                                                                                                       |                                                                                                                                                      cAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                                       <  2:1322311/151‑1 (MQ=255)   gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                     <  1:782599/151‑1 (MQ=255)     gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                                   <  1:216714/151‑1 (MQ=255)       aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGTGTTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                                                 <  1:1443353/151‑1 (MQ=255)       aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAc                                                                                                                                                  >  1:9787/1‑150 (MQ=255)          tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaaa                                                                                                                                               <  1:1543572/150‑1 (MQ=255)          tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaaa                                                                                                                                               <  2:1446548/150‑1 (MQ=255)           cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACaaaa                                                                                                                                              >  2:88606/1‑149 (MQ=255)           cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                             <  2:102969/151‑1 (MQ=255)            gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                            <  1:286744/151‑1 (MQ=255)              aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATaaa                                                                                                                                           >  1:389254/1‑148 (MQ=255)               aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAaaaaaaaaa                                                                                                                                          >  1:1270225/1‑145 (MQ=255)                 aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                                       <  2:1426931/151‑1 (MQ=255)                 aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                                       <  1:1058833/151‑1 (MQ=255)                  cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                                      <  1:693796/151‑1 (MQ=255)                    ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAAATg                                                                                                                                    >  1:625874/1‑151 (MQ=255)                        cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAAAAAAAAATAGA‑Gt                                                                                                                               >  1:237030/1‑151 (MQ=255)                        cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAATTGA‑gg                                                                                                                               >  1:395421/1‑150 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑Gtt                                                                                                                              <  1:442081/151‑1 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAAGTTTTTCCGACCAAAACaaaaaaatggggt                                                                                                                               >  1:1433310/1‑143 (MQ=255)                             ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGAGGTTGcc                                                                                                                           >  2:39270/1‑151 (MQ=255)                                gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCatg                                                                                                                        <  2:545272/150‑1 (MQ=255)                                       cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGCCATGATGAGCgg                                                                                                                >  2:1415104/1‑150 (MQ=255)                                       cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAACTTGA‑GGTGCCCTGATGAGCgg                                                                                                                >  2:880782/1‑150 (MQ=255)                                       cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGAAAAAAAAAAAAAATGGA‑GTTGGCATGATGAgggg                                                                                                                >  1:910076/1‑148 (MQ=255)                                         cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGaaa                                                                                                              <  1:1265770/151‑1 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGaaa                                                                                                              >  2:895798/1‑150 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAg                                                                                                             <  2:596984/151‑1 (MQ=255)                                           atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGt                                                                                                            <  1:1318527/151‑1 (MQ=255)                                               tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGtaat                                                                                                         <  2:1509262/150‑1 (MQ=255)                                                cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGtaata                                                                                                        <  2:426194/150‑1 (MQ=255)                                                 attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATTGAGGTTGCC‑TGATGAGGGGAAGtaataa                                                                                                       >  1:17634/1‑150 (MQ=255)                                                       aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                 <  2:957698/150‑1 (MQ=255)                                                          ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAAATGGA‑GGTGCCATGATGAGCGGAAGTAAAAAACaaaaagc                                                                                              >  2:366962/1‑145 (MQ=255)                                                          ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                              <  1:1386163/150‑1 (MQ=255)                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAATGA‑GTTGCCATTATGAGCGGAAGTAATAAGAAACAAGCAGt                                                                                           >  2:1028761/1‑151 (MQ=255)                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                           <  2:984968/151‑1 (MQ=255)                                                             gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                          <  1:1477608/151‑1 (MQ=255)                                                               gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                        <  2:1550116/151‑1 (MQ=255)                                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAATAGA‑GTTGGCATGGTGAGAGAAAAAAATAAACAACAAGCAGGGAAt                                                                                       >  1:176263/1‑150 (MQ=255)                                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATAAAAAAAAAGGA‑GTTGCCATGATGAGGGAAAGTAAAAAACAAAAAGCAGTGAATaa                                                                                     >  1:293084/1‑150 (MQ=255)                                                                      cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                                 <  2:514997/151‑1 (MQ=255)                                                                       aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                <  2:942509/151‑1 (MQ=255)                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGA‑GTTGCCATGATGAGGGAAAGAAATAAACAAAAAGCAGTGAATAAATTGACag                                                                             >  2:1133370/1‑148 (MQ=255)                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAATACAAAAAATGGA‑GTTGTCATGATGAGCGGAAGAAATAAACAAAAAGCAGGGAATAAGTTGAAag                                                                             >  1:1441511/1‑148 (MQ=255)                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                       <  1:1295846/150‑1 (MQ=255)                                                                                  aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                     >  2:172369/1‑151 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGA‑GGTGCCATGATGAGCGAAAGGAATAAACAAAAGGCAGTGAATAAGATGACAGAGATTGTCGc                                                                   >  2:1522836/1‑151 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                    <  2:1516906/150‑1 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                    <  1:649828/150‑1 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                    <  2:1280849/150‑1 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGACAAAAAAAAAAAATTGA‑GGTGTCATGATGAGGGAAAGTAATAAAAAAAAAGCAAGGAATAAATTGAAAGAGAtttttgc                                                                   >  1:535946/1‑146 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGATTTTTTTAACCAAAAACAAAAAATGGA‑GGTGGCATGATGAGCGGAAGAAAAAAAAAACAAGCAGTGGAAAAGTTGGCagaggatgacgc                                                                   >  1:892050/1‑143 (MQ=255)                                                                                     aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGA‑GTTTGCATGATGAGCGGAAGTAATAAACAAAAAGCAGGGAAAAAATTGACAGAGATTGTCGCt                                                                  >  2:1507158/1‑151 (MQ=255)                                                                                     aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAAGGA‑GGTTGCATGATGGGGGGAAAAAAAAAAAAAAAAGCAGTGAATAAAATTACAGAGAATGTTGCt                                                                  >  1:870652/1‑151 (MQ=255)                                                                                      cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATAGA‑GTTGCCATGATGGGGGGAAGGAATAAAAAACAAGGAGGGAATAAAATGACAGAGAATGTCGCt                                                                  >  1:878987/1‑150 (MQ=255)                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGA‑GTTGCCATGATGAGCGAAAGAAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                 >  2:1583912/1‑150 (MQ=255)                                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                              <  2:894302/151‑1 (MQ=255)                                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                              <  1:524574/151‑1 (MQ=255)                                                                                          ttCATGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAAATGGA‑GTTGCCATGATGAGGGGAAGAAATAAACAAAAAGCAGTGGAAAAGTTGAAAGAGATTTTTGCTAACtt                                                             >  2:1423455/1‑151 (MQ=255)                                                                                           tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                             <  1:252376/150‑1 (MQ=255)                                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGA‑GTTGCCCTGATGAGCGGAAGGAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACtttt                                                           >  2:365433/1‑150 (MQ=255)                                                                                              cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGA‑GTTGCCATGATTAGGGGAAGAAATAAACAAAAAGCAGTGAAAAAATTTACAGAGAATGTTGCTAACTTTaac                                                         >  2:641071/1‑149 (MQ=255)                                                                                               ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                        <  2:273829/151‑1 (MQ=255)                                                                                                 tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAATGG‑GTTGGCATGGTGAGGGGAAATAAAAAACAAAAAGCAGTGAAAAAAATGAGAGAGAATGTTGCTAACATTTCCGcc                                                      >  1:1430443/1‑151 (MQ=255)                                                                                                  ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                      <  2:269822/150‑1 (MQ=255)                                                                                                     aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGa                                                  <  2:720731/151‑1 (MQ=255)                                                                                                     aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAAATTTTTCAAAAAAAAAAAAAAAAAGGA‑GTTGTCATGATGAGCGGAAGTAAAAAACAAAAAGCAGTGGAAAAGTTGACCGAGAGTGTTGCTAAATTTACCCCCCTg                                                   >  1:1532081/1‑150 (MQ=255)                                                                                                        aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATAAAAAAAATTGA‑GGTGCCCTGATGAGGGGAAGTAATAAACAAAAAGCAGTGAATAAAATTACAGAGATTGTTGCTAACTTTTACCCCCTGAttt                                               >  2:116004/1‑151 (MQ=255)                                                                                                            ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGA‑GTTGCCCTTATTAGGGGAAGAAATAAACAAAAAGGAGGGGAAAAATTTACAGAGAATGTTGCTAACTTTTCAGCCATGATTTTTAc                                           >  2:1412644/1‑151 (MQ=255)                                                                                                               aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCCACAAATAAAAAAAATGGA‑GGTGCCCTGATGAGCGGAAATAATAAACAAAAAGCAGTGAATAAATTTACAGAGATTTTCGCTAACTTTTCCGCCCTGAtttttttccc                                        >  2:776709/1‑144 (MQ=255)                                                                                                                    acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAATGGG‑GGTGTCATGATGAGCGAAAGGAATAAACAAAAAGCAGTGAAAAAGATGACAGAGATTTTCGCTTACTTTACCGCCATGATTTTTTCCCCAAAGc                                   >  2:513547/1‑151 (MQ=255)                                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GGTGCCATGATGAGCGGAAGGAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGcc                                  >  2:733725/1‑151 (MQ=255)                                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGA‑GTTGTCCTGGTGAGGGGAAGGAAAAAAAAAAAAGGAGTGAATAAATTGAAAGAGAATGTCGCTAAATTTATCGCCCTGATTTTTTCCCCAATGcc                                  >  1:36797/1‑151 (MQ=255)                                                                                                                         tttAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAAAAAAAATGGG‑GGTGGCCTGGTGAGGGGAAGGAATAAAAAAAAAGCAGGGAATAAATTGACAGAGATTTTTGCTAACTTTTACGCCATGATTTTTTCCCCAAAGCCcgat                              >  1:302691/1‑151 (MQ=255)                                                                                                                         tttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGA‑GGTGCCCTGATGAGCGGAAAAAAAAAACAAAAGGCAGGGAATAAATTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTACCCGAATGCCtgat                              >  2:1054008/1‑151 (MQ=255)                                                                                                                           tAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                             <  2:931096/150‑1 (MQ=255)                                                                                                                           tAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                             <  2:561050/150‑1 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGA‑GGTGGCACGGTGAGGGGAAGTAATAAGCAAAAGGCAGCGGATAAATTGACAGAGAAGGTTGCTAACTTTTCCGCCACGATTTTTCCCCGAAAGGCtgtggac                           >  1:163655/1‑146 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAACAAAAAAAGGA‑GGTGCCATGATGAGCGGAAGTAAAAAACAACAGGCAGGGAATAAATTGACAGAGATTGTTGCCAACTTTTTCCCCCTGATTTTTACCCCAAAGCCtgctga                            >  2:1474158/1‑150 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAATAAAAAAAATGGA‑GTTGCCATGGTGAGCGAAAGTAAAAAACAACAAGCAGGGAATAAATTGAAAGAGAATTTCGCTAACTTTACCGCCCTGATTTTTACCCCAAAGCCTGATGAc                           >  2:1148484/1‑151 (MQ=255)                                                                                                                             gTGGTATTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg                          <  1:1502514/151‑1 (MQ=255)                                                                                                                                 tAGTTTGTGGCGGGGAATTTTTTCCGACAAAAACAAAAAATGGA‑GTTGCCCTGATGAGGGAAAGTAATAAGCAACAGGGAGGGAATAAATTGACAGAGATTGTTGCTAACTTTACCGCCATGATTTCTACCCGAAAGCCCGATGACggggg                      >  1:767757/1‑150 (MQ=255)                                                                                                                                   gTTTGTGGCGGTGAATTTTTTCAGAACAAAAAAAAAAAAGGG‑GGTGGCATGATGAGGGAAAAAAAAAAACAACAAGCAGTGAATAAGTTGATAGAGAATGTTGCTAAATTTTTCCCCCTGATTTTTTCCCCAATACCTGAGGAAGgggtgg                    >  1:1203196/1‑151 (MQ=255)                                                                                                                                        tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                >  2:1088677/1‑150 (MQ=255)                                                                                                                                         ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc              <  2:499381/151‑1 (MQ=255)                                                                                                                                           cGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACATGGTGGATAAACt             >  2:1098536/1‑150 (MQ=255)                                                                                                                                             gTGAATTTTTCCAGACAAATACAAAAACGGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa          <  2:389273/151‑1 (MQ=255)                                                                                                                                              tGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa          >  2:1208685/1‑150 (MQ=255)                                                                                                                                                 aTTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  1:1042511/1‑149 (MQ=255)                                                                                                                                                 aTTTTTCCAGACAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      <  1:853302/151‑1 (MQ=255)                                                                                                                                                    tttCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    >  2:422700/1‑150 (MQ=255)                                                                                                                                                      tCCAGACAAATACAAAAACGGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  <  2:235114/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206285‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑