| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,275,810 | 0 | A | T | 47.1% | 35.9 / 16.1 | 34 | I180F (ATT→TTT) | yejE | microcin C ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (6/12); new base T (16/0); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACT > NC_000913/2275666‑2275959 | gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGGCGGTCTCTTGGGGCCAAG‑GCTTTTTTaaa > 2:257483/1‑151 (MQ=255)gtgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGGAAAAACGGTCTC‑TTGGGGCAAA‑GCTTTTTTGGAg > 2:546223/1‑151 (MQ=255) tgCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAg < 2:157604/150‑1 (MQ=255) ttCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGGAAAAGGGGTCTC‑TTGGGGCAAC‑ACTTTTTTTTAGTATGGTCGGGGAt > 2:669961/1‑151 (MQ=255) ctctATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGGAAAGGCGATCTC‑TTGGGGCAAC‑GCTTTTTTGAAGTATGGGCGGGGGTgg > 2:426434/1‑150 (MQ=255) tctcGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGGTCTC‑TGGGGGCAAA‑GCTTTTTTGAAGTATGGGTGGGGGTGCCGACGCTGTTTTTTat > 2:329383/1‑151 (MQ=255) cGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGGCGATCTC‑TGGGGGCAAA‑GCTTTTTTTAAGGATGGTCGGGGGTGCCGACGCTGttttttgtttt > 2:609518/1‑145 (MQ=255) ttCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTAtttt < 1:400930/151‑1 (MQ=255) cTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTTTTACGGGGGTAAAGTCGATCTC‑TGGGGACAAC‑CCCTTTTTTAAGTATGGTGGGGGATGCCGACCCGGTTTTTTTTTAttttt > 2:72643/1‑149 (MQ=255) cTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGGTGCCGGCGCTGTTTTTTATttttttt > 2:130761/1‑149 (MQ=255) cTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGGAAAATCGGTCTC‑TTGGGGGAAA‑GCTTTTTTTGAGGATGGTGGGGGGGGCCGGCGCTGtttttttttttttttt > 1:396634/1‑149 (MQ=255) tGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGGTAAGGCGATCTC‑CTGGGGGAAA‑GCCTTTTTGTAGGATGGTCGGGGGTGCCGACGCTGGTTTTTATttttttttt > 1:95444/1‑148 (MQ=255) gTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAAACGGGCTC‑TGGGGGGAACACCTTTTTTTAAG‑ATGGGTGGGGGTGTCGACGCCGGTTTTTGTTATTTTTCtt > 1:504187/1‑151 (MQ=255) tGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTcc < 2:396635/151‑1 (MQ=255) tGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGcgt < 1:177543/151‑1 (MQ=255) aTGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAAACGAACTC‑TGGGGGCAAA‑GCTTTTTTTAAAGATGGGCGGGGGTGGCGGCGCCGttttttttttttttttttttccacggcg > 1:545558/1‑137 (MQ=255) cTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAATCGATCTC‑TGGGGGCAAC‑GCTTTTTTTGAGGATTGGCGGGGGTGCCGACGCTGTTTTTTGTTATTTTTCTTTTCCGCGGCGTa > 2:195371/1‑150 (MQ=255) tctctGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCt < 1:571523/151‑1 (MQ=255) tctctGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCt < 2:307881/151‑1 (MQ=255) ctctGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGGAAAATCGGGATC‑TTGGGGCAAC‑GCCTTATTTGAGCCTGGTCGGGGGTGGCGGCCCTGTTTTTGATTTTTTTTTTTTTCCGCGGCGGACCGccca > 1:596605/1‑149 (MQ=255) gTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGGAAAAGCGGTCTC‑CGGGGGCAAC‑CCTTTTTTTTAGGATGGGTGGGGGTGGCCGCGCTGTTTTTTGTTTTTTTTCTTTTCCGCGGCGGACAGCCCAACtt > 1:122317/1‑151 (MQ=255) ttCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACtt < 2:424277/150‑1 (MQ=255) aGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGGCGATCTC‑TGGGGGCAAC‑GCTTTTTTTAAGGATGGGCGGGGGTGCCGGCGCCGGTTTTTGTTTTTTTTCTTTTCCGCGGCGTACAGCCTAACtttttgg > 2:644546/1‑146 (MQ=255) cGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCtggtg < 1:116055/150‑1 (MQ=255) cGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAAGCGGTCTC‑TTGGGGCAAC‑GCTTTTTTGGAGGATGGGCGGGGGTGCCGGCGCTGTTTTTTATTTTTTTTCTTTTCAGCGTCGGACAGCCCAACTTCtggtgg > 2:424442/1‑151 (MQ=255) cgcTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGt < 1:183548/150‑1 (MQ=255) cTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTTTTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTg > 2:311398/1‑151 (MQ=255) cTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTTCAGCGTCGTACAGCCTAACTTCTGGTGGGTGCTGGCAATTACTGTCttttttt > 1:254056/1‑150 (MQ=255) aCAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGc < 2:384613/151‑1 (MQ=255) cggTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTc < 1:688207/151‑1 (MQ=255) tc‑tGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTgcggg > 1:44508/1‑150 (MQ=255) aC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTa < 1:324904/151‑1 (MQ=255) ttATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTa > 1:114448/1‑150 (MQ=255) ttATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTa > 1:476565/1‑150 (MQ=255) aTTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACt > 2:462291/1‑150 (MQ=255) | GTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTC‑TGGGGACAAC‑GCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACT > NC_000913/2275666‑2275959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |