Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,146	C→A	Q132K (CAG→AAG)	argP →	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,146	0	C	A	100.0%	95.2 / NA	26	Q132K (CAG→AAG)	argP	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/15);  total (11/15)

GAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAATATT  >  NC_000913/3060002‑3060281                                                                                                                                                 |                                                                                                                                        gAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGaa                                                                                                                                    >  2:200768/1‑150 (MQ=255)         cGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTgcgc                                                                                                                           <  1:95210/151‑1 (MQ=255)            ttCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGcgccg                                                                                                                         <  2:437542/150‑1 (MQ=255)               gACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCgcgg                                                                                                                      >  1:456372/1‑150 (MQ=255)                aCTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCgcggcg                                                                                                                    >  2:37667/1‑151 (MQ=255)                            ctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGgcg                                                                                                        <  2:454873/151‑1 (MQ=255)                            ctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGgc                                                                                                         <  2:174655/150‑1 (MQ=255)                             tttCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGgcg                                                                                                        >  1:63446/1‑150 (MQ=255)                                 aCTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTg                                                                                                   <  2:217199/151‑1 (MQ=255)                                      cGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTAt                                                                                              <  2:405446/151‑1 (MQ=255)                                         tCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTc                                                                                            <  1:184684/150‑1 (MQ=255)                                               ccGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATc                                                                                      <  1:457905/150‑1 (MQ=255)                                                     gTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGgcg                                                                                 >  2:179545/1‑149 (MQ=255)                                                              cGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGttg                                                                      >  1:379752/1‑151 (MQ=255)                                                                       ttCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGa                                                             >  1:91041/1‑151 (MQ=255)                                                                                tGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTgg                                                    <  2:282597/151‑1 (MQ=255)                                                                                   cTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTg                                                  <  1:294317/150‑1 (MQ=255)                                                                                        gtgtTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAATGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCg                                            >  2:196836/1‑151 (MQ=255)                                                                                              gCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTAt                                       <  2:487682/150‑1 (MQ=255)                                                                                               cTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATc                                      <  2:488889/150‑1 (MQ=255)                                                                                                       ccTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTc                              <  1:92821/150‑1 (MQ=255)                                                                                                                  cAACTTGCAGGTATAGGATGAAACCCGCACTAAGGAGCGGCTGCTCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTTTTGTCGAGAAGCTTGGTGCGATCGACTATCTGTTCGTCAGCTCAAAAcc                   <  1:423514/150‑1 (MQ=255)                                                                                                                                 ataTGAAACCCGCACTAAGAAACGTCTGCGACGAGGCAAAATGGTCGGACCGGTGAGCATTCAACATCAGGCGCTGCCGAGGTGTCTTGTCGATAAACTTGGTGCACTCGACTATATGTTCGTGAGCTCACAACCCTTTGCCGAAAAatat   >  1:169312/3‑151 (MQ=255)                                                                                                                                  gaTGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAGAACCCTTTGCCGAAAAATAtt  >  1:498920/1‑151 (MQ=255)                                                                                                                                       aaCCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGgcgc                                                                                >  2:160220/1‑68 (MQ=255)                                                                                                                                       aaCCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGgcgc                                                                                <  1:160215/68‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                        GAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAATATT  >  NC_000913/3060002‑3060281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑